Brazilian dental journalPub Date : 2024-03-22eCollection Date: 2024-01-01DOI: 10.1590/0103-6440202405665
Ellen Dionélia Alencar Rodrigues Rosa, Larissa Fernandes Vieira Da Silva, Paula Fernanda Damasceno Silva, André Luís Faria E Silva
{"title":"Color matching and color recovery in large composite restorations using single-shade or universal composites.","authors":"Ellen Dionélia Alencar Rodrigues Rosa, Larissa Fernandes Vieira Da Silva, Paula Fernanda Damasceno Silva, André Luís Faria E Silva","doi":"10.1590/0103-6440202405665","DOIUrl":"10.1590/0103-6440202405665","url":null,"abstract":"<p><p>This study assessed the color-matching ability and color recovery of unprepared teeth when using single-shade composites and a universal composite in large restorations. Buccal and palatine surface colors of molars were measured with a spectrophotometer (CIELAB) before preparing round cavities (6 mm in diameter, 2 mm in depth). The cavities were randomly filled with a single-shade composite (Omnichroma, Diamond One, or Vittra APS Unique) or a universal composite (Filtek Universal). Color measurements of the restored cavities were taken, and overall color differences (ΔEab and ΔE00) and differences in the whitening index for dentistry (ΔWID) from baseline were calculated. Additionally, visual assessments of a color match to the surrounding enamel were performed by forty evaluators (laypersons and undergraduate students of dentistry) in a viewing booth under illuminant D65, with rating scores from 0 (no color mismatch) to 4 (not acceptable). Data were analyzed using RM or one-way ANOVA (α = 0.05). Results showed that the restorations generally exhibited whiter colors (WID ranged from 27.9 to 41.3) than the unprepared teeth (WID ranged from 15.9 to 19.3). The composite Filtek Universal demonstrated the lowest color discrepancy (ΔWID = 8.6; ΔE00 = 10.8; and ΔE00 = 6.2), and no significant differences were observed among the evaluated single-shade composites. Furthermore, all composites showed similar and adequate color matches to the surrounding enamel. However, it is important to note that despite their ability to match the surrounding enamel reasonably, none of the composites evaluated in large restorations fully recovered the color observed in unprepared teeth.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e245665"},"PeriodicalIF":0.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10976307/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140308475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Brazilian dental journalPub Date : 2024-03-22eCollection Date: 2024-01-01DOI: 10.1590/0103-6440202405611
Hermano Camelo Paiva, Elaine Faga Iglecias, Laila Gonzales Freire, George Táccio de Miranda Candeiro, Basílio Rodrigues Vieira, Frederico Barbosa de Sousa, Giulio Gavini
{"title":"Root canal curvature influences uncontrolled removal of dentin and cleaning efficacy after ultrasonic activation.","authors":"Hermano Camelo Paiva, Elaine Faga Iglecias, Laila Gonzales Freire, George Táccio de Miranda Candeiro, Basílio Rodrigues Vieira, Frederico Barbosa de Sousa, Giulio Gavini","doi":"10.1590/0103-6440202405611","DOIUrl":"10.1590/0103-6440202405611","url":null,"abstract":"<p><p>This study evaluated the correlation between root canal curvature and the effects of ultrasonic irrigation in the following parameters: volume of uncontrolled dentin removal (UDRVol), maximum depth of dentin defects, removal of accumulated hard tissue debris (AHTD), and canal transportation in prepared curved root canals. Twenty-four human permanent mandibular molars were divided into two groups according to root canal curvature: moderate curvature (MC: mean 25°); and severe curvature (SC: mean 48°). The specimens were scanned using an X-ray microcomputed scanner (Skyscan 1172) before and after cleaning and shaping and after the final irrigation protocol with ultrasonic irrigation. There was a moderate correlation between the degree of root canal curvature and the volume of remaining AHTD (p<0.05) and between the degree of root canal curvature and maximum depth of defects due to uncontrolled removal of dentin (p<0.05). The teeth in the SC group had a greater maximum depth of defects on the dentin wall in the apical third than the teeth in the MC group (p <0.05). Both groups had a significant reduction of AHTD in all canal thirds, but the amount of remaining AHTD in the middle and apical thirds and the whole canal was significantly greater in the SC than in the MC group (p <0.05). Canal transportation was not influenced by the canal curvature in all thirds (p >0.05). This study concluded that root canal curvature affects significantly the uncontrolled removal of dentin and remaining AHTD volume after the final irrigation protocol with ultrasonic irrigation.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e245611"},"PeriodicalIF":0.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10976300/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140308479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Brazilian dental journalPub Date : 2023-12-22eCollection Date: 2023-01-01DOI: 10.1590/0103-6440202305582
Patricia Milagros Maquera-Huacho, Gabriel Garcia de Carvalho, Miguel Jafelicci Júnior, Elcio Marcantonio Junior, Denise Madalena Palomari Spolidorio
{"title":"Physical-chemical influences and cell behavior of natural compounds on titanium dental surfaces.","authors":"Patricia Milagros Maquera-Huacho, Gabriel Garcia de Carvalho, Miguel Jafelicci Júnior, Elcio Marcantonio Junior, Denise Madalena Palomari Spolidorio","doi":"10.1590/0103-6440202305582","DOIUrl":"10.1590/0103-6440202305582","url":null,"abstract":"<p><p>The present study evaluated the influence of carvacrol, terpinene-4-ol, and chlorhexidine on the physical-chemical properties of titanium surfaces, cell viability, proliferation, adhesion, and spreading of fibroblasts and osteoblasts in vitro. Titanium surfaces (Ti) were treated with Carvacrol (Cvc), Terpinen-4-ol (T4ol), Chlorhexidine (CHX), DMSO, and ultrapure water (Control group). Physical-chemical modifications were evaluated by surface wettability, the surface free energy (SFE) calculated from the contact angle values using the Owens-Wendt-Rabel-Kaeble (OWRK) equation, scanning electron microscopy (SEM) and energy dispersive spectrometry probe (EDS) system. Cells were seeded onto Ti-treated surfaces and incubated for 24 h and 72 h, then evaluated by Alamar blue assay and fluorescence microscopy. Surfaces treated with Cvc and T4ol showed the presence of Na, O, and Cl. All surfaces showed hydrophilic characteristics and SFE values between 5.5 mN/m and 3.4 mN/m. On the other hand, EDS peaks demonstrated the presence of O and Cl after CHX treatment. A reduction of cell viability and adhesion was noted on titanium surfaces treated with CHX after 24 and 72h. In conclusion, the results indicate that the decontamination with Cvc and T4ol on Ti surfaces does not alter the surface proprieties and allows an adequate interaction with cells involved in the re-osseointegration process such as fibroblasts and osteoblasts.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"34 5","pages":"53-62"},"PeriodicalIF":0.0,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10759949/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138833948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Brazilian dental journalPub Date : 2023-12-22eCollection Date: 2023-01-01DOI: 10.1590/0103-6440202305214
Alexandra Mena-Serrano, María G Granda-Albuja, Jenny Naranjo, Eliana Aldás Fierro, Michael Willian Favoreto, Alessandro D Loguercio, Alessandra Reis
{"title":"Effects of the application of sodium ascorbate after in-office bleaching on the penetration of hydrogen peroxide, color change, and microtensile bond strength.","authors":"Alexandra Mena-Serrano, María G Granda-Albuja, Jenny Naranjo, Eliana Aldás Fierro, Michael Willian Favoreto, Alessandro D Loguercio, Alessandra Reis","doi":"10.1590/0103-6440202305214","DOIUrl":"10.1590/0103-6440202305214","url":null,"abstract":"<p><p>This study aimed to evaluate the effects of the application of 10% sodium ascorbate (SA) after in-office bleaching on the penetration of hydrogen peroxide (HP) into the pulp chamber, color change, and microtensile bond strength (µTBS) to the resin-enamel interface. Thirty premolars and thirty molars were randomly divided into three groups (n = 20 each). One group was exposed to deionized water (negative control). The other two groups were bleached with 35% HP in a single session for 3x15 minutes for each application. However, in only one of them, SA was applied for 10 minutes after bleaching. After, the concentration (µg/mL) of HP in each pulp chamber was evaluated by UV-Vis spectrophotometry. Color changes (ΔEab, ΔE00, and ΔWID) were evaluated with a digital spectrophotometer before and in the first week after bleaching. After treatment, molars were restored and sectioned to obtain resin-enamel interface sticks for µTBS at a crosshead speed of 1 mm/min until failure. The HP concentration and µTBS data were analyzed using one-way ANOVA and Tukey tests, and color changes were analyzed by t-tests (α = 0.05). SA application significantly improved the µTBS values and reduced the HP concentrations within the pulp chambers (p < 0.0001). The application of SA significantly interfered with the color changes after bleaching when compared to the control group (p < 0.05). Application of 10% SA after in-office bleaching successfully reduced the penetration of HP into the pulp chamber; however, it decreased color change.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"34 5","pages":"87-94"},"PeriodicalIF":0.0,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10759951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138833943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Brazilian dental journalPub Date : 2023-12-22eCollection Date: 2023-01-01DOI: 10.1590/0103-6440202305264
Natália Batista Daroit, Lucas Nunes Martins, Alan Ballardin Garcia, Alex Nogueira Haas, Fábio Luiz Dal Moro Maito, Pantelis Varvaki Rados
{"title":"Oral cancer over six decades: a multivariable analysis of a clinicopathologic retrospective study.","authors":"Natália Batista Daroit, Lucas Nunes Martins, Alan Ballardin Garcia, Alex Nogueira Haas, Fábio Luiz Dal Moro Maito, Pantelis Varvaki Rados","doi":"10.1590/0103-6440202305264","DOIUrl":"10.1590/0103-6440202305264","url":null,"abstract":"<p><p>Studies have reported changes in the epidemiological profile of patients with oral cancer in recent decades, especially regarding gender and age. This study aimed to evaluate a historical series of oral malignant lesions prevalence over six decades and define characteristics associated with the occurrence, mainly, of oral squamous cell carcinoma (OSCC). A retrospective review of histopathological records from 1953 to 2019 was conducted in three oral pathology laboratories in South Brazil about age, sex, anatomical site, clinical aspect, and histopathological diagnosis. Descriptive and analytical analyses were performed comparing the histopathological diagnoses with other variables. Multivariable logistic regression was applied to determine the associated predictors of OSCC. Of the 53,065 records available in the institutions, 986 were oral malignant tumors (including all malignant neoplasms), representing 1.86% of all diagnoses. The occurrence of OSCC in the 1960's was 80.0%, decreasing over time reaching the lowest percentage of cases in the 1990's (75.8%) and significantly increasing to 88.7% in the 2010s. Females had a lower chance than males of having OSCC independently of the decade (odds ratio=0.30, p<0.001). This was the same for older individuals compared to those younger than 40 years. No interactions between sex, age, and decade were observed. The number of diagnoses of oral malignant lesions increased over time, and the occurrence of OSCC varied. Older individuals and males had higher chances of having OSCC independently of the decade. Therefore, in this study sample, no changes were observed in the epidemiological profile over the years concerning these predictors.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"34 5","pages":"115-124"},"PeriodicalIF":0.0,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10759960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138833947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Toothbrushing effects on the surface roughness and cement volume loss of bonded enamel-ceramic interface.","authors":"Luísa de Almeida Vieira Marins, Vitaliano Gomes Araújo-Neto, Beatriz Ometto Sahadi, Carolina Bosso André, Marcelo Giannini","doi":"10.1590/0103-6440202305459","DOIUrl":"10.1590/0103-6440202305459","url":null,"abstract":"<p><p>This study evaluated the effect of toothbrushing on enamel-cementing material-ceramic bonded interfaces, using different cementing materials.</p><p><strong>Materials and methods: </strong>Thirty enamel and thirty ceramic blocks were bonded with cementing materials to produce the samples that were bonded with three types of cementing materials: 1- RelyX Ultimate resin cement (REXU), 2- RelyX Unicem 2 self-adhesive resin cement (REU2) and 3- heated Z100 restorative composite (60°C). Bonded interfaces of the samples were toothbrushed and the surfaces of the 3 cementing materials were evaluated for roughness (RG, in µm), roughness profile (RP, in µm), and volume loss (VL, in µm3) (baseline and after 20,000 and 60,000 toothbrushing cycles). Data were evaluated by Generalized Linear Analysis (two factors: \"material\" and \"toothbrushing cycle\") and Bonferroni test (α=0.05).</p><p><strong>Results: </strong>REXU and Z100 exhibited lower RG than that presented by REU2, except after 60,000 toothbrushing cycles when only Z100 differed from REU2. The increase in toothbrushing cycles increased the RG and RP for all materials. REU2 also showed higher RP than those showed by REXU and Z100 when it was analyzed regarding the enamel. The VL of Z100 was the lowest with 20,000 toothbrushing cycles, regarding the enamel and ceramic. For 60,000 cycles, REXU showed the lowest VL regarding the ceramic, and REU2 had the highest VL regarding the enamel and ceramic.</p><p><strong>Conclusion: </strong>In general, REXU and Z100 showed the best results regarding the evaluations performed and the REU2 exhibited the highest RG, RP, and VL.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"34 5","pages":"63-71"},"PeriodicalIF":0.0,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10759952/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138833952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Brazilian dental journalPub Date : 2023-12-22eCollection Date: 2023-01-01DOI: 10.1590/0103-6440202305346
Mariane Carolina Faria Barbosa, Ana Luiza Peres Baldiotti, Náyra Santos Braga, Camila Takáo Lopes, Saul Martins Paiva, Ana Flávia Granville-Garcia, Fernanda de Morais Ferreira
{"title":"Cross-cultural adaptation of the Digital Health Literacy Instrument (DHLI) for use on Brazilian adolescents.","authors":"Mariane Carolina Faria Barbosa, Ana Luiza Peres Baldiotti, Náyra Santos Braga, Camila Takáo Lopes, Saul Martins Paiva, Ana Flávia Granville-Garcia, Fernanda de Morais Ferreira","doi":"10.1590/0103-6440202305346","DOIUrl":"10.1590/0103-6440202305346","url":null,"abstract":"<p><p>The present study aimed to perform the cross-cultural adaptation of the Digital Health Literacy Instrument (DHLI) for native Brazilian Portuguese-speaking adolescents (DHLI-BrA). Cross-cultural adaptation consisted of the following steps: translation, assessment, and adjustments by the expert committee to ensure cultural equivalence; back-translation, and synthesis of back-translations. Cognitive testing was then performed in a pretest with adolescents using cognitive interviews with probing questions on the item's understanding interpretation and response options. Cronbach's alpha coefficient and McDonald's omega were used to estimate the instrument's reliability. Forty-two Brazilian adolescents participated in the study (mean age: 16.0 ± 2.0 years; range: 13 to 19 years). Items that were difficult to understand were adapted to the context of Brazilian adolescents. Cronbach's alpha coefficient and McDonald's omega for the 21 items of the DHLI-BrA were, respectively, 0.79 and 0.80. Cronbach's alpha coefficient for the subscales of the self-report instrument was 0.53-0.79 (range), demonstrating good reliability in the total instrument and moderate reliability in the subscales. This study provides the cross-cultural adapted version of the Digital Health Literacy Instrument (DHLI), which is an instrument for measuring digital Health literacy, for use in Brazilian adolescents (DHLI-BrA).</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"34 5","pages":"104-114"},"PeriodicalIF":0.0,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10759948/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138833941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Brazilian dental journalPub Date : 2023-12-22eCollection Date: 2023-01-01DOI: 10.1590/0103-6440202305508
Cibele Sales Rabelo, Juliana Marinho Ramos de Oliveira, Isabelly de Carvalho Leal, Flávia de Miranda Leão Leite Costa, Nágila Maria Pontes Silva Ricardo, Vanara Florêncio Passos
{"title":"The potential of galactomannan from Caesalpinia ferrea on erosive dentin wear reduction in vitro.","authors":"Cibele Sales Rabelo, Juliana Marinho Ramos de Oliveira, Isabelly de Carvalho Leal, Flávia de Miranda Leão Leite Costa, Nágila Maria Pontes Silva Ricardo, Vanara Florêncio Passos","doi":"10.1590/0103-6440202305508","DOIUrl":"10.1590/0103-6440202305508","url":null,"abstract":"<p><p>Gels containing juca seed galactomannan (JSG) were evaluated for their potential to prevent the progression of dentin erosive wear in an in vitro study with four experimental groups (n = 9). The treatments included distilled water (DW), 0.05% stannous fluoride (121 ppm F), and 0.5% or 1% JSG. The specimens underwent a cycle (3 times/day) consisting of immersion in 1% citric acid (5 minutes), treatment (5 minutes), and artificial saliva exposure (2 hours/overnight) for 5 days. Surface changes were assessed using mechanical profilometry (wear), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDS). The data were analyzed using ANOVA followed by Tukey's post-test (p < 0.05). The negative control group exhibited the highest wear (6.0 µm ± 3.5), significantly differing from the group treated with 0.05% stannous fluoride gel (p = 0.007), which showed less dentin loss. The groups treated with 0.5% and 1% JSG showed results similar to the negative control (p = 0.661; p = 0.212, respectively) and the stannous fluoride group (p = 0.103; p = 0.379, respectively). In the SEM images, the specimen treated with stannous fluoride showed obliterated tubules, while the JSG gels formed crystals on the dentin surface, as confirmed by the presence of oxygen and calcium in the EDS analysis. Although the JSG gels showed similar results to the stannous fluoride, did not exhibit superior efficacy at the tested concentrations.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"34 5","pages":"72-78"},"PeriodicalIF":0.0,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10759959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138833951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Brazilian dental journalPub Date : 2023-12-22eCollection Date: 2023-01-01DOI: 10.1590/0103-6440202305496
Kaíssa da Cunha Lima, Rocio Geng Vivanco, Pedro Roberto Braz Rodrigues, Ana Lúcia Pereira Caetano, Fernanda de Carvalho Panzeri Pires-de-Souza
{"title":"Long-term effect of firing protocols on surface roughness and flexural strength of lithium disilicate glass-ceramic.","authors":"Kaíssa da Cunha Lima, Rocio Geng Vivanco, Pedro Roberto Braz Rodrigues, Ana Lúcia Pereira Caetano, Fernanda de Carvalho Panzeri Pires-de-Souza","doi":"10.1590/0103-6440202305496","DOIUrl":"10.1590/0103-6440202305496","url":null,"abstract":"<p><p>This study evaluated the effect of different firing protocols on the surface roughness and flexural strength of CAD/CAM lithium disilicate glass-ceramic (LD) after aging methods. Forty-two LD bars of 16 x 4 x 2 mm (IPS e-max CAD, Ivoclar) were randomly separated into two groups according to firing protocols: Single firing-Staining, glazing, and crystallization in a single step; Multiple firings-Crystallization+First staining+Firing+Second staining+Firing+Glazing+Firing. After protocols, initial surface roughness readings were taken (Surfcorder SE1700, Kosakalab). Samples were then randomly separated into three groups (n=7) according to the aging methods they were submitted: Thermomechanical cycling (TMC, ER System, Erios, 1,200,000 cycles, 0.3 MPa, 2 Hz and 5°C/37°C/55°C, 30 s swell time); Simulated toothbrushing (STB, Pepsodent, MAVTEC, 73,000 cycles), and Control (no aging). Final surface roughness readings were done, and samples were submitted to a three-point bending test (OM100, Odeme Dental Research) and fractographic analysis by scanning electron microscopy (EVO-MA10, ZEISS). Data were analyzed (2-way ANOVA, (α=.05). There was no difference (p>.05) in the flexural strength between the firing protocols, regardless of the aging method. STB decreased the flexural strength of samples submitted to multiple firings, different from control (p<.05). Without aging (Control), before TMC, and after STB, LD had lower surface roughness when submitted to multiple firings than to single firing (p<.05). The firing protocols did not affect the flexural strength or the surface roughness of the lithium disilicate glass-ceramic, even after aging. However, toothbrushing negatively affected the flexural strength and smoothed the surface of the ceramic submitted to multiple firings.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"34 5","pages":"79-86"},"PeriodicalIF":0.0,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10759956/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138833946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Brazilian dental journalPub Date : 2023-12-22eCollection Date: 2023-01-01DOI: 10.1590/0103-6440202305036
Ondina Karla Mousinho Rocha Torres, Luiz Arthur Barbosa da Silva, Roseana de Almeida Freitas, Éricka Janine Dantas da Silveira, Márcia Cristina da Costa Miguel
{"title":"Immunoexpression of HSP27 does not seem to influence the prognosis of oral tongue squamous cell carcinoma.","authors":"Ondina Karla Mousinho Rocha Torres, Luiz Arthur Barbosa da Silva, Roseana de Almeida Freitas, Éricka Janine Dantas da Silveira, Márcia Cristina da Costa Miguel","doi":"10.1590/0103-6440202305036","DOIUrl":"10.1590/0103-6440202305036","url":null,"abstract":"<p><p>New methods of early detection and risk assessment have been studied aiming to predict the prognosis of patients and directing a specialized treatment of the oral tongue squamous cell carcinoma (OTSCC). In this context, several molecular biomarkers have been investigated for this purpose, and, among them, the heat shock protein 27 (HSP27) can be named. The study aimed to analyze whether heat shock protein 27 (HSP27) exerts any influence on OTSCC, correlating its immunoexpression with clinicopathological parameters, and patient survival. The sample comprised 55 OTSCC cases and 20 normal oral mucosa specimens. The malignancy grading systems proposed by the WHO in 2005, Brandwein-Gensler et al., and Almangush et al. were applied in a histomorphological study. HSP27 expressions were evaluated through the Immunoreactivity Score System (IRS). Significant values were considered at p <0.05 for all statistical tests. Higher IRS results were observed for normal oral mucosa specimens when compared to OTSCC cases (p <0.001). No significant associations between HSP27 immunostaining, the analyzed clinicopathological parameters and patient survival were observed. The results of the present study indicate lower HSP27 expression in OTSCC cases compared to normal oral mucosa specimens. Thus, HSP27 expression does not seem to influence patient prognosis.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"34 5","pages":"125-133"},"PeriodicalIF":0.0,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10759947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138833945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}