Brazilian dental journal最新文献

{"title":"Minimally Invasive Access in Endodontics: A National Survey of Brazilian Specialists.","authors":"Fabienne de Freitas Rodrigues Moraes, Roberta Fonseca de Castro, Emmanuel João Nogueira Leal da Silva, Francisco Montagner, Juliana Melo da Silva Brandão","doi":"10.1590/0103-644020266955","DOIUrl":"10.1590/0103-644020266955","url":null,"abstract":"<p><p>This study aims to evaluate Brazilian endodontists' perceptions of minimally invasive access cavities, characterize access-design choices, and identify the information sources informing these decisions. This cross-sectional survey randomly sampled endodontists registered with the Federal Council of Dentistry in 2021. Consenting participants completed a previously validated questionnaire. A minimum sample of 376 was calculated (assumed proportion 50%; margin of error 5%). Descriptive statistics summarized responses, and Pearson's chi-square tested associations between access type and covariates (α = 0.05). Of 378 respondents, most reported using traditional access for anterior (55.8%) and posterior teeth (66.4%). On a 0-5 scale, the modal self-rated knowledge of minimally invasive access cavities was 4 (28%). Most agreed/strongly agreed that minimally invasive access cavities: increases fracture resistance (49.5%); hinders canal location (84.4%); impairs instrument centering during preparation (73.5%); raises the risk of apical transportation (43.4%) and iatrogenic deviations (63.0%); contributes to debris accumulation (60.1%); compromises pulp-chamber cleaning (74.6%); elevates the likelihood of instrument fracture (68.3%); and increases the risk of coronal discoloration (57.6%). Scientific articles were the most frequently cited source of professional updating (44.2%). Brazilian endodontists perceived multiple procedural risks associated with minimally invasive access cavities and often believed it improves fracture resistance, a view not supported by current evidence. These findings reveal a perception-evidence gap and underscore the need to strengthen critical appraisal and evidence integration in education and clinical decision-making on access cavity design.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"37 ","pages":"e256955"},"PeriodicalIF":0.0,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13502928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148842705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of light output from light-curing units used in a public oral health service in Brazil.","authors":"Laryssa Silva da Cunha, Airin Karelys Avendaño Rondon, Maryuri Del Carmen Macias Bautista, Ianca Daniele Oliveira de Jesus, Guilherme Mendonça Benoni, Gisele Rodrigues da Silva, Richard Bengt Price, Carlos José Soares","doi":"10.1590/0103-644020267001","DOIUrl":"10.1590/0103-644020267001","url":null,"abstract":"<p><p>This observational field study aimed to evaluate the radiant power (mW), emission spectra (mW/cm²/nm), irradiance (mW/cm²), and the light tip integrity of 40 light-curing units (LCUs) used in 16 public oral health service units in the city of Uberlândia, Minas Gerais, Brazil. The radiant power and emission spectrum were measured using a spectrometer attached to an integrating sphere. Irradiance profiles were obtained using a Beam Profiler. The tips were photographed and classified in 4 categories: A. undamaged, B. contaminated by resin residue, C. damaged, and D. contaminated and damaged. Data were analyzed by one-way ANOVA (α = 0.05), and the emission spectra and beam profiles were analyzed descriptively. The LCUs tested were single-peak light-emitting diode units, and 85% of the LCUs had damaged light tips. The radiant power values were significantly different between the LCUs (P < 0.001) and ranged between 94.2 and 588.2 mW. The irradiance ranged from 255.8 to 1597.8 mW/cm². The LCU tip diameter ranged from 6.1 to 8.3 mm. Two-dimensional beam profiles show that the light distribution was not homogeneous across the tips, and an irradiance peak was often observed at the center of the LCUs with smaller tips. Only 15% of the LCU were undamaged, reflecting substantial variability in radiant power and irradiance delivered. Although no statistically significant differences were observed among tip condition categories, these conditions showed a trend toward reduced and inhomogeneous light output.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"37 ","pages":"e257001"},"PeriodicalIF":0.0,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13502927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148842690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing Gingival Smile and Muscle Strength.","authors":"Karinne Bueno Antunes, Luiz Eduardo Monteiro Dias da Rocha, Ramon Silva Dos Santos, Lucio Souza Gonçalves, Ricardo Guimarães Fischer","doi":"10.1590/0103-644020266640","DOIUrl":"10.1590/0103-644020266640","url":null,"abstract":"<p><p>A gummy smile (GS) may result from dental, skeletal, or muscular etiologies, with lip hyperactivity (LH) being the most prevalent. Currently, lip muscle strength assessment relies on empirical methods, compromising the precision of certain treatments. This study aimed to evaluate muscle strength related to smiling, establish correlations between lip movement and both individual and total muscle force, and determine the prevalence of GS etiologies. A device was developed to measure the strength of each lip muscle during smiling. A total of 89 individuals (mean age 28 ± 8.4 years; 80.9% female) were divided into four groups: G1 - Upper lip hyperactivity (9%); G2 - Dental/skeletal causes (19.1%); G3 - Control (33.7%); G4 - Hyperactivity combined with other etiologies (38.2%). Statistical analysis included ANOVA, Tukey's post hoc, and chi-square tests. LH, alone or combined, was the most prevalent etiology (68.2%) and presented 30% greater muscle strength than other groups. G4 exhibited significantly higher strength across all six muscles evaluated compared to G2 and G3. The zygomaticus major muscle in G1 was significantly stronger than in G2 and G3. Lip translation exhibited significant differences in force based on displacement (p <0.001), with the most substantial force observed from 8 mm of displacement. LH was identified as the primary etiology of GS, with individuals displaying LH showing greater muscle strength compared to other etiologies or controls.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"37 ","pages":"e256640"},"PeriodicalIF":0.0,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13502926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148842675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Torque expression in conventional and self-ligating orthodontic brackets: An in vitro study.","authors":"Martha de Abreu Avila, José Guilherme Neves, Américo Bortolazzo Correr, Milton Santamaria-Junior, Eduardo Almada, Lourenço Correr-Sobrinho, Ana Rosa Costa","doi":"10.1590/0103-644020266914","DOIUrl":"10.1590/0103-644020266914","url":null,"abstract":"<p><p>This study assessed torque expression at different angulations of conventional, interactive, and passive self-ligating brackets, with or without metal ligatures, and evaluated surface morphology and chemical composition (%). All brackets (Roth prescription, 0.022\" x 0.028\" slot) were fixed to round acrylic cylinders with cyanoacrylate adhesive. Five hundred segments of rectangular archwires (0.019\" x 0.025\") were bent and welded (20 mm x 10 mm), then fixed to the brackets either with or without 0.010\" metal ligatures in ten experimental groups (n = 10). The assembled specimens were then mounted on a universal testing machine, and a vertical force was applied to the free end of the archwire, resulting in a 10 mm displacement. Mean torque values (N·mm) at specified torque angles of 0° to 25°, as well as the mean torque angles (°) corresponding to 5 and 20 N·mm, were analyzed using ANOVA and Tukey's test (α = 0.05). Bracket design and chemical composition were examined by SEM/EDS. Slot angulation and dimensional measurements were evaluated using ImageJ. Passive self-ligating brackets, without and with ligatures, showed the smallest torque angles, statistically smaller than those of the other groups (p < 0.001). Bracket clips were composed predominantly of nickel and titanium. Passive self-ligating brackets, either ligated or not, showed the highest torque expression at both 5 and 20 N·mm. Overall, torque expression was influenced more by the dimensional characteristics of brackets and rectangular archwires than by the type of ligature used. Clinically, passive self-ligating brackets may facilitate earlier torque expression during orthodontic treatment.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"37 ","pages":"e256914"},"PeriodicalIF":0.0,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13502929/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148842702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of zinc-modified niobium nanoparticles incorporation in resinous monomers on mechanical properties.","authors":"Larissa Luri Almeida Amorim Ikejiri, Marilia Mattar de Amoêdo Campos Velo, Nair Cristina Margarido Brondino, Carlos Alberto Spironelli Ramos, Ana Paula de Melo Alves Guedes, Tatiana Rita de Lima Nascimento, Rafael Francisco Lia Mondelli","doi":"10.1590/0103-644020266930","DOIUrl":"10.1590/0103-644020266930","url":null,"abstract":"<p><p>This in vitro study evaluated the influence of the incorporation of different concentrations of zinc-modified niobium nanoparticles (Nb/Zn) on the mechanical properties of a flowable resin composite (OptiFlow II, LC/Pac-Dent) by surface hardness (SH), depth of cure (DoC), flexural strength (FS), and modulus of elasticity (E). The morphology and composition of Nb/Zn nanoparticles were evaluated by Scanning Electron Microscopy and energy-dispersive X-ray spectroscopy (EDX). The Nb/Zn nanoparticles were incorporated at 3 different concentrations (0.3%; 0.6% e 0.9%), resulting in four groups: OptiFlowII, OptiFlow II+0.3%Nb/Zn, OptiFlow II+0.6%Nb/Zn, and OptiFlow II+0.9%Nb/Zn. SH was measured at the top and bottom of the specimens (n=6/group). FS and E were conducted using a Universal Testing Machine (Instron, 50N) (n=14/group). For SH, DoC, and FS, ANOVA followed by Tukey's test was used for statistical analyses, and for E, pairwise comparisons were conducted using a sandwich covariance matrix (p<0.05). The results showed that OptiFlow II+0.6%Nb/Zn exhibited the lowest top-SH values in all comparisons (p=0.003). Regarding DoC, the data indicated a significant difference between the groups with 0.3% and 0.6% Nb/Zn (p=0.04). For E, OptiFlow II+0.3%Nb/Zn presented higher values (E=3.48±0.15)a compared to OptiFlow II+0.6%Nb/Zn (E=3.3±0.18; p=0.02)b, OptiFlowII presented higher values (E=3.56±0.27)a than OptiFlow II+0.6%Nb/Zn (p=0.01) and OptiFlow II+0.9%Nb/Zn (E=3.3±0.27; p=0.02)b. Thus, the incorporation of Nb/Zn nanoparticles at different concentrations into the OptiFlow II affects its mechanical properties, without altering the depth of cure, but reducing flexural strength. The group with the lowest nanoparticle concentration showed E similar to that of the control group and higher than that of the higher-concentration groups. Overall, the incorporation of Nb/Zn nanoparticles influenced the mechanical behavior of the flowable resin composite. Lower concentrations performed similarly to the control, while higher levels impaired properties, highlighting the need for optimized nanoparticle loading.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"37 ","pages":"e256930"},"PeriodicalIF":0.0,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13449945/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148725940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Wetting Agents on Microhardness, Color, and Gloss of a Nanohybrid Composite after Aging.","authors":"Valeria Bisinoto Gotti, Rafael Martins Chagas, Isabela Torres Resende, João Vitor da Silva Duarte, Marcella Bessa Palhares, Analia Gabriella Borges Ferraz Facury, César Penazzo Lepri, Vinícius Geraldo Martins, Ana Paula Ayres Oliveira","doi":"10.1590/0103-644020266842","DOIUrl":"10.1590/0103-644020266842","url":null,"abstract":"<p><p>This study investigated whether wetting agent application alters surface microhardness, gloss, and color stability of a nanohybrid resin composite after simulated aging by brushing or thermocycling. Sixty discs of one nanohybrid resin composite were fabricated and divided into three conditions: no wetting agent, Wetting Resin (WR), or Modeling Resin (MR). Baseline evaluations included Knoop microhardness, color using the CIE Lab* system, and 60° gloss. Aging was performed by either standardized brushing or 10,000 thermocycles (5-55 °C). Post-aging values were compared with baseline data. Statistical analysis used two-way repeated-measures ANOVA/Tukey for microhardness, and nonparametric tests for gloss and ΔE (α = 0.05). Wetting agent application lowered baseline microhardness compared to controls, with MR showing the lowest values. Thermocycling reduced gloss, while brushing increased gloss in WR and maintained it in MR. Sealants also affected color stability, with material- and method-dependent responses. In conclusion, the wetting agent influenced the mechanical and optical behavior of the nanohybrid resin composite in a manner dependent on the agent and aging protocol. These materials reduced surface hardness and produced distinct gloss and color responses to brushing versus thermocycling.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"37 ","pages":"e256842"},"PeriodicalIF":0.0,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13449965/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148722975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reactivation capacity of TiO2 nanoparticles for dental bleaching: an in vitro study.","authors":"Suely Cristina Aragão Veras Dos Santos, Tainah Oliveira Rifane, Isabel Silva do Nascimento, Lucca Reis Mesquita, Nayara Oliveira de Souza, Madiana Magalhães Moreira, Victor Pinheiro Feitosa","doi":"10.1590/0103-644020266525","DOIUrl":"10.1590/0103-644020266525","url":null,"abstract":"<p><p>This study evaluated the bleaching efficacy, pH behavior, and reuse potential of a titanium dioxide nanoparticle (TiO₂) suspension as a photocatalytic, peroxide-free alternative to carbamide peroxide (CP) for at-home bleaching. Forty bovine incisors were subjected to a 21-day at-home bleaching regimen (2 h/day) using fresh or reused agents (n = 10): fresh CP (16% CP), reapplied CP (16% CP collected and reused), fresh TiO₂ (10 wt% TiO₂ suspension), and reapplied TiO₂ (reactivated 10 wt% TiO₂ suspension). TiO₂ suspensions were UV-activated daily (385 nm, 50 min), and the reused TiO₂ was reactivated prior to each application. The pH of the bleaching agents was assessed before gel application and after completion of the at-home protocol; all experimental groups showed a similar pH trend, with values increasing from 6.0 to 7.0 throughout the procedure. Bleaching efficacy was evaluated using CIEDE2000 (ΔE₀₀) and whiteness index (ΔWID) values obtained with a digital spectrophotometer (VITA Easyshade V). Data were analyzed with one-way ANOVA and Tukey's test (α = 0.05). ΔE₀₀ analysis showed that fresh TiO₂ produced the lowest color change, with values closest to the perceptibility and acceptability thresholds, differing significantly from reapplied TiO₂, fresh CP, and reapplied CP (p < 0.05). No significant differences were observed among the remaining groups, and all other conditions exhibited ΔE₀₀ values above the acceptability threshold. ΔWID values did not differ significantly among groups (p = 0.475). The UV-activated TiO₂ suspension (100 min total exposure) was able to bleach teeth without peroxide.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"37 ","pages":"e256525"},"PeriodicalIF":0.0,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13449946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148727259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Diamond-Coated Ultrasonic Tip Finishing on Resin Cement Bond Strength to Dentin.","authors":"Leonardo Alvares Sobral Silva, Giovanni de Souza Goulart Cardoso, Lucas Tadeu Pereira Dos Santos, Clarisse Maria Luiz Silva, Matheus de Paula Costa, Milton Santamaria Junior, João Maurício Ferraz da Silva","doi":"10.1590/0103-644020266906","DOIUrl":"10.1590/0103-644020266906","url":null,"abstract":"<p><p>This study aimed to evaluate the effect of dentin surface finishing protocols using ultrasonic CVD diamond tips on the bond strength of resin cement to dentin. Seventy-two bovine incisor crowns were prepared and assigned to four dentin surface finishing protocols (Groups 1-4, n = 18), including a conventional control group and experimental groups using ultrasonic CVD diamond tips applied sequentially with increasing fineness. One specimen per group was analyzed by scanning electron microscopy to assess surface topography and smear layer characteristics. The samples were bonded using light-cured resin cement in combination with three adhesive strategies: total-etch, self-etch, and universal adhesives. After 24 hours of storage in distilled water, microshear bond strength testing was performed (0.5 mm/min). Data were analyzed using two-way ANOVA and Tukey's post hoc test (α = 0.05). Groups 3 and 4 showed significantly higher bond strength than the control group (p < 0.05), while no significant differences were observed among adhesive strategies (p > 0.05). Surface analysis revealed that these protocols produced a more homogeneous and regular smear layer, whereas intermediate finishing resulted in a more irregular surface. It can be concluded that the sequential use of ultrasonic CVD diamond tips with increasing fineness improves the bond strength of resin cement to dentin and promotes a more favorable substrate for adhesion.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"37 ","pages":"e256906"},"PeriodicalIF":0.0,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13449944/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148724205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Addition of silver nanoparticle solutions to alginate: microbiological and mechanical analysis.","authors":"Larissa Paulino de Figueiredo, Analia Gabriella Borges Ferraz Facury, Eduardo Martinelli Franco, Gilberto Antonio Borges, Roberto Nascimento Silva, Lourenço Correr-Sobrinho, José Guilherme Neves, Ana Rosa Costa","doi":"10.1590/0103-644020266966","DOIUrl":"10.1590/0103-644020266966","url":null,"abstract":"<p><p>This study assesses the antimicrobial activity and physical alterations of alginate impression material in the presence of silver nanoparticle (AgNP) solutions. The samples were assigned to five groups according to the type of alginate and solution: (JW) Jeltrate + distilled water; (JCHX) Jeltrate + 0.2% chlorhexidine digluconate; (JAgNP_0.2%) Jeltrate + 0.2% AgNP; (JAgNP_1%) Jeltrate + 1% AgNP; and (AW) Avagel + distilled water. AgNPs were synthesized from the fungus Trichoderma reesei. Fifty impressions were made (n=10) from a matrix, from which plaster models were fabricated. Surface detail reproduction and dimensional stability were analyzed under a light microscope. Surface detail reproduction was achieved by replicating a 50µm line on the plaster model. Dimensional stability was calculated from measurements of X' and X'' in the matrix and plaster models. The antimicrobial effect was assessed independently by measuring the formation of zones of inhibition around the alginate molds (n=3) in agar medium containing Streptococcus mutans and Candida albicans. Dimensional stability values were subjected to the ANOVA and Tukey's post-hoc test (α=0.05). JCHX showed the highest dimensional stability and differed significantly from the other groups (p<0.05). A 50µm line was reproduced in the plaster for all tested groups, regardless of the impression material and antimicrobial solution. AgNPs incorporated into the alginate mixture did not produce dimensional changes in the alginate mold. AW and JCHX exhibited antimicrobial potential, as evidenced by bacterial inhibition. Jeltrate or Avagel, regardless of AgNP concentration, did not demonstrate antimicrobial activity.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"37 ","pages":"e256966"},"PeriodicalIF":0.0,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13459399/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148725423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histology and immune impact in OSCC.","authors":"Andressa Fernanda Paza Miguel, Nicole Lonni, Túlio Silva Rosa, Daniella Serafin Couto Vieira, Gustavo Davi Rabelo, Elena Riet Correa Rivero","doi":"10.1590/0103-644020267030","DOIUrl":"10.1590/0103-644020267030","url":null,"abstract":"<p><p>This study investigated how tumour histopathological characteristics and immune cell phenotypes in the tumour microenvironment influence the prognosis of patients with oral squamous cell carcinoma. Thirty-one samples of oral squamous cell carcinoma were analysed using immunohistochemistry to assess tumour budding, perineural invasion, depth of invasion, tumour thickness, lymphovascular invasion, pattern of invasion, and tumour-stroma ratio. Anti-CD66b and anti-CD8 immunostaining were used to examine neutrophil and lymphocyte infiltration within the tumour core, invasive front, and overall tumour. Kaplan-Meier survival curves were employed to correlate these features with patient survival. High tumour budding was observed in lesions with perineural and lymphovascular invasion and was positively associated with tumour thickness. Dispersed tumours had the highest depth of invasion and thickness, while non-cohesive tumours exhibited more perineural invasion and greater CD66b+ cell infiltration at the invasive front. Increased CD66b+ counts, non-cohesive and dispersed patterns, were associated with shorter overall survival. A higher ratio of neutrophils to lymphocytes was observed in patients who died. In conclusion, high tumour budding, perineural and lymphovascular invasion, and increased CD66b+ infiltration at the invasive front are linked to aggressive tumour behaviour and poor prognosis. These features may serve as important predictors of patient outcomes.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"37 ","pages":"e257030"},"PeriodicalIF":0.0,"publicationDate":"2026-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13405571/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148623544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}