Brazilian dental journal最新文献

Influence of Bioceramic Clinker particle size, radiopacifier, and liquids on their physicochemical properties. 生物陶瓷熟料粒度、放射性混浊剂和液体对其理化性质的影响。

Brazilian dental journal Pub Date : 2025-04-14 eCollection Date: 2025-01-01 DOI: 10.1590/0103-644020256326

Karina Ines Medina Carita Tavares, Airton Oliveira Santos-Junior, Jáder Camilo Pinto, Fernanda Ferrari Esteves Torres, Marco Antonio Hungaro Duarte, Juliane Maria Guerreiro-Tanomaru, Mário Tanomaru-Filho

{"title":"Influence of Bioceramic Clinker particle size, radiopacifier, and liquids on their physicochemical properties.","authors":"Karina Ines Medina Carita Tavares, Airton Oliveira Santos-Junior, Jáder Camilo Pinto, Fernanda Ferrari Esteves Torres, Marco Antonio Hungaro Duarte, Juliane Maria Guerreiro-Tanomaru, Mário Tanomaru-Filho","doi":"10.1590/0103-644020256326","DOIUrl":"https://doi.org/10.1590/0103-644020256326","url":null,"abstract":"This study evaluated the addition of calcium tungstate (CaWO4) as a radiopacifier and the manipulation with distilled water (DW) or liquid with additives (LA) on the physicochemical properties of Clinker (CL; Angelus) with different particle sizes (2 to 30 µm and <2 µm), compared to White MTA and MTA Repair HP. Initial and final setting times (ST) were evaluated according to ISO 6876:2012 and ASTM C266-03 standards. Radiopacity was assessed following ISO guidelines, and solubility was evaluated after immersion in distilled water or phosphate-buffered saline for 7 days. The pH was measured using a digital pH meter in 1, 3, 7, 14, 21, and 28 days. ANOVA and Tukey and unpaired t-tests were performed (α=0.05). CL 2 to 30 µm showed shorter initial ST and higher final ST when manipulated with LA (p<0.05). The addition of CaWO4 increased the CL radiopacity for both particle sizes (p<0.05) but did not influence ST, solubility, and pH (p>0.05). All materials presented alkaline pH (p>0.05), especially in 24 hours and 7 days. Regarding the solubility, all groups exhibited mass gain, while White MTA in distilled water demonstrated mass loss (p<0.05). CL 2 to 30 µm + CaWO4 showed greater mass gain and higher radiopacity when manipulated with LA than DW (p<0.05). CL <2 µm manipulated with LA provides a shorter final ST. Both particle sizes positively influence the alkalization capacity of CL, especially in the initial periods. The addition of calcium tungstate and manipulation using LA showed adequate physicochemical properties for clinical application.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"36 ","pages":"e246326"},"PeriodicalIF":0.0,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11996162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144057257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Individual and contextual factors associated with orofacial dysfunction in schoolchindren. 与学龄期儿童口面部功能障碍相关的个体和环境因素。

Brazilian dental journal Pub Date : 2025-04-14 eCollection Date: 2025-01-01 DOI: 10.1590/0103-644020256228

Luiza Jordânia Serafim de Araújo, Monalisa Cesarino Gomes, Ramon Targino Firmino, Edja Maria Melo de Brito Costa, Saul Martins de Paiva, Ana Flávia Granville-Garcia

{"title":"Individual and contextual factors associated with orofacial dysfunction in schoolchindren.","authors":"Luiza Jordânia Serafim de Araújo, Monalisa Cesarino Gomes, Ramon Targino Firmino, Edja Maria Melo de Brito Costa, Saul Martins de Paiva, Ana Flávia Granville-Garcia","doi":"10.1590/0103-644020256228","DOIUrl":"https://doi.org/10.1590/0103-644020256228","url":null,"abstract":"To investigate individual and contextual factors associated with orofacial dysfunction in schoolchildren. A cross-sectional study was conducted with 739 children eight to ten years of age. The children answered questionnaires addressing orofacial dysfunction and anxiety. Guardians provided information on sociodemographic characteristics, sleep disturbances, and family cohesion. Examiners investigated the presence of orofacial dysfunction, dental caries, malocclusion, and traumatic dental injuries (Andreasen criteria) (Kappa>0.80). The type of school and average monthly income of the school neighborhood were the contextual variables. Descriptive statistics were performed to characterize the sample. Unadjusted and adjusted (p <0.05) multilevel Poisson regression models were run. The prevalence of orofacial dysfunction was 33.3%. After adjustment by the contextual variables, a lower family income, a larger quantity of carious teeth, the presence of traumatic dental injury, severe/very severe malocclusion, the presence of sleep disturbances, and anxiety remained associated with orofacial dysfunction, whereas definite malocclusion was a protection factor. In terms of context, attending a public school was associated with orofacial dysfunction. Orofacial dysfunction was influenced by a lower family income, a larger quantity of carious teeth, and the presence of traumatic dental injury, sleep disturbances, and anxiety. Moreover, attending a public school was the contextual determinant that played a significant role in the outcome.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"36 ","pages":"e246228"},"PeriodicalIF":0.0,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11996160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143996932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Titanium Particles Released from Dental Implants Under Fluoride Exposure Interact with Macrophages. 氟暴露下牙种植体释放的钛颗粒与巨噬细胞相互作用

Brazilian dental journal Pub Date : 2025-04-14 eCollection Date: 2025-01-01 DOI: 10.1590/0103-644020256187

Waad Kheder, Soumya Sheela, A R Samsudin, Sausan Al Kawas, Nadia Khalifa, Ali Qabbani

{"title":"Titanium Particles Released from Dental Implants Under Fluoride Exposure Interact with Macrophages.","authors":"Waad Kheder, Soumya Sheela, A R Samsudin, Sausan Al Kawas, Nadia Khalifa, Ali Qabbani","doi":"10.1590/0103-644020256187","DOIUrl":"https://doi.org/10.1590/0103-644020256187","url":null,"abstract":"This study is designed to investigate the influence of fluoride and pH value on the release of titanium particles from Ti6Al4V dental implants with hydrophobic microrough surface produced by sandblasting and acid-etching techniques; and correlate particle size to their uptake by macrophages and expression of inflammatory cytokines. Fifteen dental implants were immersed in five test solutions with different fluoride concentrations and pH values. Three control implants were scanned using a scanning electron microscope and fifteen test implants were also scanned after their immersion in the test solutions. The immersion solutions were analyzed for titanium particles/ions size-range and amount. The uptake of titanium particles by macrophages and expression of Il-1 β and IL-8 following their exposure to titanium particles were investigated. Test solutions with high fluoride and acidity resulted in the release of micro-size titanium particles (4551.7 ± 114.5 nm and 2783 ± 101.13 nm); while those with low fluoride, neutral pH, and alkaline environment resulted in the release of nano-size titanium particles (431.2 ± 80.6 nm, 448.3 ± 112 nm, and 484.5 ± 85.3 nm respectively). There was an increase in the uptake of nanoparticles by macrophages without altering their membrane integrity. The increase in expression of IL-1β and IL-8 by M0 macrophages after exposure to titanium dioxide particles may facilitate our understanding of immune cell population-specific molecular events deriving the peri-implant inflammation in response to titanium particles. Fluoride and pH values influence the release of titanium particles from the implant's surface. The activated inflammatory mediators are key to imbalance in osteoblast-osteoclast activity and failure of implant osseointegration.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"36 ","pages":"e246187"},"PeriodicalIF":0.0,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11996161/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144048830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enamel Deproteinization with Sodium Hypochlorite: Effects on Ceramic Bracket Bond Strength. 次氯酸钠脱釉对陶瓷支架粘结强度的影响。

Brazilian dental journal Pub Date : 2025-04-14 eCollection Date: 2025-01-01 DOI: 10.1590/0103-644020256106

Priscila Cordeiro, José Guilherme Neves, Midori Tsuzuki, Anália Gabriela Ferraz Facury, Lourenço Correr-Sobrinho, Ana Rosa Costa

{"title":"Enamel Deproteinization with Sodium Hypochlorite: Effects on Ceramic Bracket Bond Strength.","authors":"Priscila Cordeiro, José Guilherme Neves, Midori Tsuzuki, Anália Gabriela Ferraz Facury, Lourenço Correr-Sobrinho, Ana Rosa Costa","doi":"10.1590/0103-644020256106","DOIUrl":"https://doi.org/10.1590/0103-644020256106","url":null,"abstract":"To assess the efficiency of different concentrations of sodium hypochlorite (NaOCl) on the bonding of ceramic brackets to dental enamel using different bonding protocols. Ceramic, a polycrystalline bracket, was bonded to 90 extracted bovine incisors using primer + Transbond XT (P+TXT); Optibond S adhesive + Transbond XT (OS+TXT), and OS + Orthocem (OS+OC). Three concentrations of NaOCl (0%, 2.5%, and 5.25%) were used for each material, resulting in nine experimental groups (n=10). The shear bond strength (SBS) assay was performed on a universal testing machine. The surface morphology of the enamel and tooth/bonding material interface was examined under a scanning electron microscope. Kruskal-Wallis and Dunn's tests were performed to compare the non-parametric data (ARI scores). SBS data were analyzed using a generalized linear model. All analyses were performed using the R program and with a significance level of 5%. No significant differences in SBS between the groups with different concentrations of NaOCl (p>0.05). OS+OC group yielded the lowest bond strength values (3.35 MPa_0%, 4.90 MPa_2.5%, 4.68 MPa_5.25%) compared to the other groups, regardless of NaOCl (p<0.05). No significant difference between the materials regarding the ARI score (p>0.05). OS+TXT-5.25% showed significantly higher scores (14.4 MPa) than OS+TXT-2.5% (9.17 MPa) (p<0.05). The surface morphology analysis revealed a change in the etching pattern for types 1 and 2 when NaOCl was used. The different concentrations of NaOCl previously applied onto the enamel surface did not enhance bond strength. OS+OC should not be recommended for bonding with fixed ceramic orthodontic devices.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"36 ","pages":"e246106"},"PeriodicalIF":0.0,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11996157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144061386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Psychometric properties of the Brazilian version of the Patient Satisfaction Questionnaire. 巴西版患者满意度问卷的心理测量特性。

Brazilian dental journal Pub Date : 2025-04-14 eCollection Date: 2025-01-01 DOI: 10.1590/0103-644020256206

Luisa Gatti-Reis, Renata Negreiros Alvarenga, Xiangqun Ju, Lisa Jamieson, Lucas Guimarães Abreu, Saul Martins Paiva

{"title":"Psychometric properties of the Brazilian version of the Patient Satisfaction Questionnaire.","authors":"Luisa Gatti-Reis, Renata Negreiros Alvarenga, Xiangqun Ju, Lisa Jamieson, Lucas Guimarães Abreu, Saul Martins Paiva","doi":"10.1590/0103-644020256206","DOIUrl":"https://doi.org/10.1590/0103-644020256206","url":null,"abstract":"This study aimed to assess the psychometric properties of the Brazilian version of the Patient Satisfaction Questionnaire (PSQ) which assesses the satisfaction of adolescents with orthodontic treatment. The PSQ is a 58-item self-report instrument presenting six subscales (doctor-patient relationship, situational aspects, dentofacial improvement, psychosocial improvement, dental function, and a residual category). The psychometric properties of the Brazilian version of the PSQ (B-PSQ) were assessed in a cross-sectional study with 111 adolescents between 11-18 years who had undergone orthodontic treatment. The sample's features and the questionnaire's characteristics (including the determination of the floor and ceiling effect) were assessed with descriptive statistics. The instrument's internal consistency was assessed by Cronbach's alpha and stability with the intraclass correlation coefficient (ICC) in test-retest reliability. The convergent and discriminant construct validity was also assessed. Data included responses from 106 adolescents, of which 58 (54.7%) were female. The percentage of individuals reaching the floor effect was 0% in all subscales. In four subscales, the percentage of individuals reaching the ceiling effect was <15%. The B-PSQ showed excellent reliability for the total score (Cronbach's alpha=0.919; ICC=0.918), ranging from good to excellent for all subscales. B-PSQ's total score exhibited a high correlation with all five subscales (ρ>0.50). Scores of young adolescents (10-14 years) were significantly higher in the dental function (p=0.018) and situational aspects (p=0.003) subscales, showing the discriminant validity of the instrument. The B-PSQ has good psychometric properties regarding stability and appears to be a valuable instrument to assess the satisfaction of Brazilian adolescents with orthodontic treatment.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"36 ","pages":"e246206"},"PeriodicalIF":0.0,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11996159/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144004418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bond strength between acrylic resin and artificial tooth treated with experimental silane with thio-urethane. 实验硅烷与硫氨基甲酸乙酯处理的丙烯酸树脂与人工牙的粘结强度。

Brazilian dental journal Pub Date : 2025-04-14 eCollection Date: 2025-01-01 DOI: 10.1590/0103-644020256136

Leonardo Guimarães Sonehara, Carmem Silvia Pfeifer, Ana Paula Piovezan Fugolin, Mario Alexandre Coelho Sinhoreti, Rafael Leonardo Xediek Consani

{"title":"Bond strength between acrylic resin and artificial tooth treated with experimental silane with thio-urethane.","authors":"Leonardo Guimarães Sonehara, Carmem Silvia Pfeifer, Ana Paula Piovezan Fugolin, Mario Alexandre Coelho Sinhoreti, Rafael Leonardo Xediek Consani","doi":"10.1590/0103-644020256136","DOIUrl":"https://doi.org/10.1590/0103-644020256136","url":null,"abstract":"This in vitro study evaluated the bond strength between thermo-activated acrylic resin and artificial tooth treated with experimental silane incorporated with thio-urethane. Artificial molar teeth were individually fixed in 50mm long cylindrical wax sticks and traditionally included in metal flasks with type III plaster coated with laboratory silicone. The tooth/wax sets were removed from the flask, the tooth separated from the wax stick, and the fitting area was cleaned with household detergent. Teeth were separated into three groups according to fitting area treatments (n=10): CON (Control, no treatment or silane application), ABR (Abrasion with diamond tip), and JAT (Blasting with 50μm aluminum oxide particles). Tooth fitting area treatment was associated with PALABOND commercial silane application (PS) or experimental silane incorporated with thio-urethane (ES). Teeth were replaced in the plaster mold, and the acrylic resin traditionally flask pressed and polymerized in a heated water bath. Tooth/resin sets were individually fixed in rigid PVC tubes with chemically activated acrylic resin, leaving a space of 1 mm between the tooth/PVC tube top, and submitted to the shear strength test in a universal test machine. Shear strength data were evaluated for normality, and subjected to one-way ANOVA and Tukey's test (5%). JAT+PS showed greater strength followed by ABR+ES. CON and JAT+ES showed lowest values and ABR+PS was intermediate. In conclusion, the artificial teeth treatments with experimental silane incorporated with thio-urethane associated with blasting or abrasion promoted different strength values when bonded to acrylic resin for denture base.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"36 ","pages":"e246136"},"PeriodicalIF":0.0,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11996154/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144059012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro evaluation of the enhancement of glass ionomer cement features by using chitosan and nanodiamond. 壳聚糖和纳米金刚石对玻璃离子水泥性能增强的体外评价。

Brazilian dental journal Pub Date : 2025-04-14 eCollection Date: 2025-01-01 DOI: 10.1590/0103-644020246215

Murilo Henrique Torres da Silva, Henrico Badaoui Strazzi-Sahyon, Renata Gallo, Ticiane Cestari Fagundes, André Luiz Fraga Briso, Victoria Tamara Perchyonok, Paulo Henrique Dos Santos

{"title":"In vitro evaluation of the enhancement of glass ionomer cement features by using chitosan and nanodiamond.","authors":"Murilo Henrique Torres da Silva, Henrico Badaoui Strazzi-Sahyon, Renata Gallo, Ticiane Cestari Fagundes, André Luiz Fraga Briso, Victoria Tamara Perchyonok, Paulo Henrique Dos Santos","doi":"10.1590/0103-644020246215","DOIUrl":"https://doi.org/10.1590/0103-644020246215","url":null,"abstract":"This study investigated the influence of chitosan and nanodiamond incorporation on the surface, optical, and mechanical properties of glass ionomer cement. Total 56 samples (5 mm diameter and 2 mm thickness) were prepared and divided into 4 groups according to the incorporation of chitosan and nanodiamond on Fuji II glass ionomer cement: Control group: no incorporation; 10%CH group: incorporation of 10% chitosan; 10%ND group: incorporation of 10% of nanodiamond; 5%CH-5%ND group: incorporation of 5% chitosan and 5% nanodiamond (n=14). Analyses of color stability, surface roughness, fluorescence intensity, microhardness, morphology, and chemical composition were investigated. Additionally, water sorption, hygroscopic expansion, contact angle, surface free energy, and total free energy of interaction were also assessed. After the initial readings, the samples were individually stored in red wine solution for 28 days. Data were subjected to ANOVA followed by Tukey´s test (α=.05). Aging in wine solution altered the optical, mechanical, and surface properties of glass ionomer cement regardless of the incorporation of the compound (P<.05). 10% chitosan-incorporated glass ionomer cement promoted higher color alteration, surface roughness, and water sorption after aging (P<.05). 10% nanodiamond-incorporated glass ionomer cement showed higher microhardness compared to the other groups before aging (P<.05), however there were no differences among them after aging (P>.05). In general, no differences between the 5% chitosan- and 5% nanodiamond-incorporated glass ionomer cement and control groups were noted on the evaluated analyses (P>.05). Thus, the incorporation of 5% chitosan and 5% nanodiamond is a satisfactory alternative for maintain the surface, optical, and mechanical properties of the glass ionomer cement.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"36 ","pages":"e246215"},"PeriodicalIF":0.0,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11996155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144065558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of wooden spatula and plastic pipette methods for caries risk assessment in infants and toddlers. 木铲法和塑料移液法在婴幼儿龋齿风险评估中的评价。

Brazilian dental journal Pub Date : 2025-04-14 eCollection Date: 2025-01-01 DOI: 10.1590/0103-644020256407

Silvina Tineo, Paulo Nelson-Filho, Thais Citolino Barbosa, José Maria Alvarez Gimenez, Raquel Assed Bezzera da Silva, Lea Assed Bezzera da Silva, Marta Estela Saravia

{"title":"Evaluation of wooden spatula and plastic pipette methods for caries risk assessment in infants and toddlers.","authors":"Silvina Tineo, Paulo Nelson-Filho, Thais Citolino Barbosa, José Maria Alvarez Gimenez, Raquel Assed Bezzera da Silva, Lea Assed Bezzera da Silva, Marta Estela Saravia","doi":"10.1590/0103-644020256407","DOIUrl":"https://doi.org/10.1590/0103-644020256407","url":null,"abstract":"The study aimed to compare two different methods (wooden spatula and plastic pipette) for the collection of unstimulated saliva for colony counting of Mutans Streptococci species (MS) (microbiological caries risk), in infants and toddlers. The children's behavior was favorable (very comfortable or comfortable) and unfavorable (uncomfortable or very uncomfortable), while the saliva collection, was also evaluated. Saliva samples were obtained from 19 children aged 1-29 months, of both sexes and seeded by both methods, obtaining the MS CFU numbers. The ANOVA test was used to statistically analyze the microbiological results, and the Z-test and chi-square test were used to analyze the behavioral assessment (α= 0,05%). 63.1% and 57.9% of children had MS in their saliva, using the saliva collection techniques with a spatula and a pipette, respectively. The number of CFUs was an average of 10.47 for saliva collected with the spatula and 7.32 for saliva collected with the pipette, however, there was no statistical difference between the methods (p=0.696653). Comparing the ages 1-6 months and 18-29 months, the older children showed higher CFU numbers, for both methods (p=0.000383). The clinical assessment of the child's behavior demonstrated a significant statistical difference between the two methods, with more positive behavior for the spatula (p<0.001). In conclusion, the wooden spatula method can be used for saliva collection and quantifying of the MS levels in infants and toddlers, since there was no significant difference in the CFUs count, furthermore is better accepted based on the child's behavior, compared to the plastic pipette technique.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"36 ","pages":"e246407"},"PeriodicalIF":0.0,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11996158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144039687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytotoxicity and bioactivity of CIMMO DTA restorative cement in human activated-dental pulp cells in vitro. CIMMO - DTA修复骨水泥对体外活化牙髓细胞的细胞毒性和生物活性研究。

Brazilian dental journal Pub Date : 2025-04-14 eCollection Date: 2025-01-01 DOI: 10.1590/0103-644020256402

Iandara de Lima Scardini, Karollyne Dos Santos Spigariol, Marcelo Dos Santos, Carla Renata Sipert, Letícia Martins Santos

{"title":"Cytotoxicity and bioactivity of CIMMO DTA restorative cement in human activated-dental pulp cells in vitro.","authors":"Iandara de Lima Scardini, Karollyne Dos Santos Spigariol, Marcelo Dos Santos, Carla Renata Sipert, Letícia Martins Santos","doi":"10.1590/0103-644020256402","DOIUrl":"https://doi.org/10.1590/0103-644020256402","url":null,"abstract":"Bioceramic materials have been introduced in dentistry due to their physical, chemical, and biological properties, which are considered advanced in clinical practice. According to the manufacturer, CIMMO DTA bioceramic restorative cement is a biological bioceramic cement and was introduced recently at a low cost compared to other bioceramic materials. However, no studies evaluated the effect of CIMMO DTA on human dental pulp cells (HDPC) activated with lipopolysaccharide (LPS). Therefore, this study aimed to evaluate the cytotoxicity and the osteo/odontogenic differentiation potential in HDPC activated with LPS exposed to CIMMO DTA compared to Hydro-C. HDPC activated or not with LPS were cultured, plated, and exposed to different dilutions of CIMMO DTA and Hydro-C extracts. At 24h, 48h, and 72h, the cells were subjected to the MTT assay. The osteo/odontogenic mineralization potential of cells was evaluated through the Alizarin red S staining assay at a 21-day time point. Statistical analysis was performed using a two-way analysis of variance (ANOVA) followed by Tukey's post-test (p<0.05). In general, the tested materials unaffected the metabolic activity and viability of cells in all dilutions evaluated. The CIMMO DTA reduced the osteo/odontogenic differentiation potential of the HDPC according to qualitative and quantitative results at dilutions of 1:8. Therefore, the results of the present study suggested that CIMMO DTA presents limited bioactive potential on HDPC in vitro.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"36 ","pages":"e246402"},"PeriodicalIF":0.0,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11996156/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144034483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Papain associated with root planing in treatment of periodontitis. 木瓜蛋白酶与牙周炎治疗中牙根刨平相关。

Brazilian dental journal Pub Date : 2025-04-07 eCollection Date: 2025-01-01 DOI: 10.1590/0103-644020255813

Luiz Eduardo Monteiro Dias da Rocha, Ricardo Guimarães Fischer

{"title":"Papain associated with root planing in treatment of periodontitis.","authors":"Luiz Eduardo Monteiro Dias da Rocha, Ricardo Guimarães Fischer","doi":"10.1590/0103-644020255813","DOIUrl":"10.1590/0103-644020255813","url":null,"abstract":"Although subgingival scaling and root planing (SRP) are the \"gold standard\" treatment for periodontitis, these processes may remove excessive cement, exposing dentin and causing hypersensitivity, or producing defects such as ridges and grooves, leaving residual calculus and preventing access to the whole of the root surface. A papain gel that removes carious dentin (Papacárie®) has been introduced and this gel may also help to remove supra and subgingival calculus, acting on its organic portion and biofilm, decreasing the consumption of cement and generating lower levels of aerosol compared to SRP. The aim of the present split-mouth, single-blind study was thus to compare the effectiveness of papain gel with subgingival root planning (RP). After receiving oral hygiene instruction, supragingival scaling, and coronary polishing, 18 periodontitis patients (6 women and 12 men, mean age of 51 ± 8 years) were included in the study. Treatment involved subgingival application of the gel for one minute, followed by RP while the control group received subgingival SRP. Treatment was conducted by three operators and a blinded examiner recorded the following clinical parameters at baseline and after three months: plaque index (PI), bleeding on probing (BoP), probing pocket depth (PPD), clinical attachment level (CAL), and gingival recession (GR). The results showed significant improvements in clinical parameters, in both the treatment and the control group, with no statistical difference between them. It was concluded that papain did not provide any additional.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"36 ","pages":"e255813"},"PeriodicalIF":0.0,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11981590/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143805266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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