Brazilian dental journal最新文献_第2页

Biological Properties of Bioceramic Sealers on Osteoblastic Cells: A Comparative Study. 生物陶瓷封口处成骨细胞生物学特性的比较研究。

Brazilian dental journal Pub Date : 2024-12-16 eCollection Date: 2024-01-01 DOI: 10.1590/0103-6440202406037

Angelita Piovezana Guerra, Danielle Gregorio, Gean Carlos Yamamoto, Nathalia Thalitha Bernardes Dos Santos, Regina Celia Poli-Frederico, Luciana Prado Maia

{"title":"Biological Properties of Bioceramic Sealers on Osteoblastic Cells: A Comparative Study.","authors":"Angelita Piovezana Guerra, Danielle Gregorio, Gean Carlos Yamamoto, Nathalia Thalitha Bernardes Dos Santos, Regina Celia Poli-Frederico, Luciana Prado Maia","doi":"10.1590/0103-6440202406037","DOIUrl":"10.1590/0103-6440202406037","url":null,"abstract":"This study aimed to assess the biological properties of two ready-to-use bioceramic sealers (EndoSequence BC Sealer - EBCS; Bio-C Sealer - BCS) on osteoblastic lineage cells. MC3T3 osteoblast-like cells were exposed to extracts of bioceramic materials. Cytotoxicity was evaluated using the MTT method, genotoxicity was assessed by the micronucleus test and the expression of BMP1, BMP2 and ALP was measured by RT-qPCR, after 1, 3 and 7 days. Wound healing was monitored at 1, 2 and 3-day intervals using the scratch test. Statistical analysis involved a two-factor ANOVA, followed by the Tukey Test for multiple comparisons (α=0.05). Cytotoxicity assessment revealed no significant differences between the materials and the control group at any of the time points, indicating that neither material exhibited cytotoxic effects. However, both cements induced greater micronuclei formation compared to the control on days 1 and 7 (p<0.05) when evaluating genotoxicity. No significant differences between the groups were observed in wound healing at any of the time intervals. Both biomaterials upregulated the expression of BMP1, BMP2 and ALP. The two bioceramic sealers exhibited comparable biological properties, including cytocompatibility, promotion of wound healing, and upregulation of bone formation-related genes in osteoblast-like cells. While these results suggest the potential for safe clinical application, the observed genotoxicity warrants caution. Therefore, while the null hypothesis-that the two bioceramic sealers exhibit analogous properties-was accepted in terms of cytocompatibility and gene expression, further investigation is essential to fully ensure their safety and efficacy in bone-related procedures.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e246037"},"PeriodicalIF":0.0,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11654336/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142856748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Does the pH of the grape seed extract interfere in the biomodification capacity of dentin collagen? 葡萄籽提取物的pH值是否会干扰牙本质胶原蛋白的生物修饰能力？

Brazilian dental journal Pub Date : 2024-12-16 eCollection Date: 2024-01-01 DOI: 10.1590/0103-6440202406048

Marcelo Victor Sidou Lemos, Gabriela Araújo Lourenço, Samuel Chillavert Dias Pascoal, Talita Arrais Daniel Mendes, Sérgio Lima Santiago

{"title":"Does the pH of the grape seed extract interfere in the biomodification capacity of dentin collagen?","authors":"Marcelo Victor Sidou Lemos, Gabriela Araújo Lourenço, Samuel Chillavert Dias Pascoal, Talita Arrais Daniel Mendes, Sérgio Lima Santiago","doi":"10.1590/0103-6440202406048","DOIUrl":"10.1590/0103-6440202406048","url":null,"abstract":"The aim of this study was to verify the biomodifying action of 6.5% grape seed extract solutions, with different pH, when applied on dentin collagen. Dentin bars (1.7 mm x 6.0 mm x 0.5 mm) were demineralized for 5 hours in 10% phosphoric acid, and distributed into the following groups: acid solution (pH=4.42); neutral (pH=6.96); alkaline (pH=11.92) and distilled water (pH=6.75). Three-point flexural test (n=10) and mass variation (n=10) were assessed at different periods (baseline, after biomodification, 7 and 14 days of remineralizing solution storage). For qualitative analysis, similar dentin bars were prepared and analyzed using Fourier transform infrared spectroscopy (FT-IR) and Raman Spectroscopy (FT-Raman) before and after 1 hour of immersion in biomodifying solution with different pH. Data were subjected to Shapiro-Wilk normality tests, followed by two-way ANOVA for repeated measures and Tukey's post-test (p<0.05). Alkaline solution was effective in increasing the modulus of elasticity, showing a decrease after 7 days and subsequent stabilization after 14 days of storage. Acid solution group showed a greater increase in the modulus of elasticity immediately after biomodification, but it was not stable over storage. Regarding mass variation, only the acid solution showed an increase in mass after biomodification and 14 days of storage. In relation to FT-IR and FT-Raman, all solutions showed interaction with collagen at some level. Therefore, pH of the solution directly influences the action of the grape seed extract, with satisfactory results being found in both acidic and alkaline solutions.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e246048"},"PeriodicalIF":0.0,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11654020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optical properties and color stability of a translucent zirconia: effects of finishing after grinding. 一种半透明氧化锆的光学性质和颜色稳定性：研磨后精加工的影响。

Brazilian dental journal Pub Date : 2024-12-16 eCollection Date: 2024-01-01 DOI: 10.1590/0103-6440202406094

Pablo Machado Soares, Camila Pauleski Zucuni, Bruna Dias Ilha, Leticia Borges Jacques, Liliana Gressler May, Luiz Felipe Valandro

{"title":"Optical properties and color stability of a translucent zirconia: effects of finishing after grinding.","authors":"Pablo Machado Soares, Camila Pauleski Zucuni, Bruna Dias Ilha, Leticia Borges Jacques, Liliana Gressler May, Luiz Felipe Valandro","doi":"10.1590/0103-6440202406094","DOIUrl":"10.1590/0103-6440202406094","url":null,"abstract":"This study evaluated the effect of grinding and finishing treatments (polishing or glaze) on the color stability, translucency and opalescence of a translucent zirconia (3Y-TZP) after immersion in red wine. Discs (Ø= 12 mm; thickness 0.8 mm) of 3Y-TZP (Zenostar T, Ivoclar AG) were randomly allocated (n= 8) according to the surface treatment factor: Ctrl - as-sintered; Gr - grinding with diamond bur #4219; Gr + Pol - grinding followed by polishing; Gr + Gl - grinding followed by glaze. Surface roughness and scanning electron microscopy were carried out. All specimens were subjected to daily immersions in red wine for 30 minutes during 18 days. The color differences (ΔE00) were measured prior, after 9 and 18 days of immersion through the CIEDE2000 formula, as same as translucency (TP00) and opalescence (OP) parameters. Kruskal Wallis, repeated measures ANOVA/Tukey, and Spearman's correlation tests were performed to evaluate the roughness, optical and correlation data, respectively. The Gr + Gl depicted the lowest ΔE00 value after 9 days. The Gr group showed the greatest ΔE00 values in all immersion times, whereas the other groups presented similar behavior after 18 days. The Gr and Gr + Pol groups had the highest Ra and Rz values. The correlation between roughness and color change was moderate and significant (p= 0.02, r= 0.42). No differences were observed for TP00 and OP. The surface treatments affected the roughness and color stability of zirconia, being glaze application more effective to reduce color alterations after the immersion protocol.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e246094"},"PeriodicalIF":0.0,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11653993/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Repopulation of a 3D simulated periapical lesion cavity with dental pulp stem cell spheroids with triggered osteoblastic differentiation. 引发成骨细胞分化的牙髓干细胞球体三维模拟根尖周围病变腔的重建。

Brazilian dental journal Pub Date : 2024-12-16 eCollection Date: 2024-01-01 DOI: 10.1590/0103-644020235847

Vítor Luís Ribeiro, Janaína A Dernowsek, Roger R Fernandes, Dimitrius L Pitol, João Paulo Mardegan Issa, Jardel F Mazzi-Chaves, Karina Fittipaldi Bombonato-Prado, Manoel Damião Sousa-Neto, Geraldo Aleixo Passos

{"title":"Repopulation of a 3D simulated periapical lesion cavity with dental pulp stem cell spheroids with triggered osteoblastic differentiation.","authors":"Vítor Luís Ribeiro, Janaína A Dernowsek, Roger R Fernandes, Dimitrius L Pitol, João Paulo Mardegan Issa, Jardel F Mazzi-Chaves, Karina Fittipaldi Bombonato-Prado, Manoel Damião Sousa-Neto, Geraldo Aleixo Passos","doi":"10.1590/0103-644020235847","DOIUrl":"10.1590/0103-644020235847","url":null,"abstract":"We established a proof-of-concept model system for the biological healing of periapical lesions using stem cell spheroids. Mesenchymal stem cells from human exfoliated deciduous teeth (SHED) were cultured in a 2D monolayer and then as 3D multicellular spheroids. An image of a periapical lesion of an upper lateral incisor tooth was obtained by computed tomography and was used as a model for photopolymer resin 3D printing to generate a negative frame of the lesion. The negative model served to prepare a positive model of the periapical lesion cavity in an agarose gel. SHED that were cultured in monolayers or as spheroids were seeded in the positive lesion mold before or after osteoblastic differentiation. The results showed that compared to cells cultured in monolayers, spheroids exhibited uniform cellularity and a greater viability within the lesion cavity, which was accompanied by a temporal reduction in the expression of CD13, CD29, CD44, CD73, and CD90 mRNAs that are typically expressed by stem cells. Concomitantly, the expression of markers that characterize osteoblastic differentiation (RUNX2, ALP, and BGLAP) increased. These results provide a new perspective for regenerative endodontics with the use of SHED-derived spheroids to repair periapical lesions.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e235847"},"PeriodicalIF":0.0,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11654009/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rho Kinases and Reactive Oxygen Species in Autophagy Regulation by Pressure in Periodontal Ligament Cells.

Brazilian dental journal Pub Date : 2024-12-06 eCollection Date: 2024-01-01 DOI: 10.1590/0103-6440202405944

Miriam Hardt, Alexandra Mayr, Eric Kutschera, Jana Marciniak, Erika Calvano Küchler, Christian Kirschneck, James Deschner, Andreas Jäger, Svenja Beisel-Memmert

{"title":"Rho Kinases and Reactive Oxygen Species in Autophagy Regulation by Pressure in Periodontal Ligament Cells.","authors":"Miriam Hardt, Alexandra Mayr, Eric Kutschera, Jana Marciniak, Erika Calvano Küchler, Christian Kirschneck, James Deschner, Andreas Jäger, Svenja Beisel-Memmert","doi":"10.1590/0103-6440202405944","DOIUrl":"10.1590/0103-6440202405944","url":null,"abstract":"Autophagy is a self-digestion mechanism of cells, which is related to cell stress. It enables cell survival by maintaining cellular homeostasis or initiates cell death. This study aimed to investigate the intracellular signaling of pressure-induced autophagy regulation in human periodontal ligament (PDL) cells and to analyze the involvement of Rho kinases (ROCK) and reactive oxygen species (ROS) in particular. Human PDL cells were treated with the ROCK inhibitor Y-27632 and the ROS scavenger N-acetylcysteine (NAC) in combination with pressure magnitudes of 2, 6, and 8 g/cm2 over 16 hours. Cells treated with rapamycin served as a positive control and untreated cells as a control group. The Cyto-ID® Autophagy Detection Kit was used for flow cytometric analysis. Statistical analysis was performed using ANOVA and post-hoc tests. The results show that the pressure-induced autophagy was affected differently by the two inhibitors (p<0.05). The application of Y-27632 led to a significant reduction in autophagy in all pressure groups. The application of NAC led to reduced autophagy at pressures of 2 g/cm2 and 6 g/cm2. At 8 g/cm2, this effect was no longer present. In the control group, autophagy was significantly reduced by Y-27632 and significantly increased by NAC. Our data suggest that both Rho-kinase and reactive oxygen species could influence pressure-induced autophagy regulation in PDL cells.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e245944"},"PeriodicalIF":0.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11653791/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Economic Evaluation of Community Water Fluoridation in an Upper-Middle-Income Country.

Brazilian dental journal Pub Date : 2024-12-06 eCollection Date: 2024-01-01 DOI: 10.1590/0103-644020245994

Lorrayne Belotti, Paulo Frazão

{"title":"Economic Evaluation of Community Water Fluoridation in an Upper-Middle-Income Country.","authors":"Lorrayne Belotti, Paulo Frazão","doi":"10.1590/0103-644020245994","DOIUrl":"10.1590/0103-644020245994","url":null,"abstract":"The aim was to estimate the cost-effectiveness and cost-benefit of CWF for schoolchildren according to different population sizes served in Brazil. The economic evaluation was conducted from a societal perspective. Total costs were estimated using four variables across different population sizes: capital cost of initial installation, chemical product costs, system operational costs, and monitoring costs. The effect of CWF was analyzed in the context of the widespread use of fluoridated toothpaste, based on studies with Brazilian population groups. The total cost of dental treatment was estimated, including both direct and indirect costs, with a discount rate of 3.5%. A one-way sensitivity analysis was conducted to test the robustness of the results based on measured parameter values. The costs averted due to CWF were US$174.40 and US$85.67 for children aged 5-8 and 3-12 years, respectively, and US$46.66 for those aged 7-12 years, according to the average effectiveness of CWF for each age group. The cost-effectiveness and cost-benefit ratio results were favorable in all scenarios where the population served was 6,000 or more inhabitants. Scenarios unfavorable to CWF were observed only in populations of up to 2,000 inhabitants. The economic evaluation of CWF in an upper-middle-income country proved to be a cost-effective oral health intervention and more economically advantageous, especially in larger areas for both deciduous and permanent dentition.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e245994"},"PeriodicalIF":0.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11654337/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Frontal Sinus Dimensions According to the Skeletal Malocclusion in German Teenagers.

Brazilian dental journal Pub Date : 2024-12-06 eCollection Date: 2024-01-01 DOI: 10.1590/0103-644020245964

Maria Beatriz Carvalho Ribeiro de Oliveira, Guido Artemio Marañón-Vásquez, Isabela Ribeiro Madalena, Eva Paddenberg-Schubert, Svenja Beisel-Memmert, César Penazzo Lepri, Ângela Graciela Deliga Schroder, Peter Proof, Christian Kirschneck, Erika Calvano Küchler, Maria Angélica Hueb de Meneze-Oliveira

{"title":"Analysis of Frontal Sinus Dimensions According to the Skeletal Malocclusion in German Teenagers.","authors":"Maria Beatriz Carvalho Ribeiro de Oliveira, Guido Artemio Marañón-Vásquez, Isabela Ribeiro Madalena, Eva Paddenberg-Schubert, Svenja Beisel-Memmert, César Penazzo Lepri, Ângela Graciela Deliga Schroder, Peter Proof, Christian Kirschneck, Erika Calvano Küchler, Maria Angélica Hueb de Meneze-Oliveira","doi":"10.1590/0103-644020245964","DOIUrl":"10.1590/0103-644020245964","url":null,"abstract":"The present study explored the association between the craniofacial sagittal skeletal patterns and frontal sinus dimensions in Germans. This cross-sectional study included orthodontic patients with age ranging from 11 to 18 (80 males, 82 females). Lateral cephalograms were used in the cephalometric and frontal sinus analysis. Cephalometric analysis was performed to measure SNA, SNB and ANB angles. Frontal sinus measurements were performed to obtain the frontal sinus height, antero-posterior dimension and frontal sinus lateral projection area. The frontal sinus dimensions were compared between genders with Mann Withney test. Kruskal Wallis and Dunn's post-hoc tests were used to compare the mean differences between the skeletal malocclusions. Spearman's correlation test and linear regression model were also performed. Statistical significance was set at 5%. Regarding skeletal malocclusion, 71 patients presented class I, 81 class II and 10 class III skeletal malocclusion. The frontal sinus height (p=0.009), frontal sinus antero-posterior dimension (p=0.001) and frontal sinus lateral projection area (p=0.007) were bigger in males than in females. The frontal sinus antero-posterior dimension was significantly greater in the class III when compared to the class I (p=0.010) and class II (p=0.027). Frontal sinus lateral projection area was also bigger in class III than class I (p=0.039). In the linear regression model a significant association was observed between frontal sinus lateral projection area and class III (skeletal class I as a reference). In conclusion, our result suggests that the frontal sinus lateral projection area might present morphometric differences in German teenagers with skeletal class III malocclusion.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e245964"},"PeriodicalIF":0.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11653665/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of Maxillary Sinus Reactions and Periapical Pathology in the Maxillary Posterior Teeth: Evaluation Using Cone Beam Computed Tomography.

Brazilian dental journal Pub Date : 2024-12-06 eCollection Date: 2024-01-01 DOI: 10.1590/0103-644020245973

Alba Elizabeth do Nascimento Gomes, Carlos Eduardo da Silveira Bueno, Alexandre Sigrist De Martin, Carolina Pessoa Stringheta, Carlos Eduardo Fontana, Daniel Guimarães Pedro Rocha, Ana Grasiela Limoeiro, Wayne Martins Nascimento, Marilia Fagury Videira Marceliano-Alves, Rina Andrea Pelegrine

{"title":"Association of Maxillary Sinus Reactions and Periapical Pathology in the Maxillary Posterior Teeth: Evaluation Using Cone Beam Computed Tomography.","authors":"Alba Elizabeth do Nascimento Gomes, Carlos Eduardo da Silveira Bueno, Alexandre Sigrist De Martin, Carolina Pessoa Stringheta, Carlos Eduardo Fontana, Daniel Guimarães Pedro Rocha, Ana Grasiela Limoeiro, Wayne Martins Nascimento, Marilia Fagury Videira Marceliano-Alves, Rina Andrea Pelegrine","doi":"10.1590/0103-644020245973","DOIUrl":"10.1590/0103-644020245973","url":null,"abstract":"This study aimed to investigate the association between maxillary sinus reactions and periradicular pathology in maxillary posterior teeth using cone-beam computed tomography. The maxillary posterior images of 395 teeth were examined for the presence of periradicular pathology, and the maxillary sinus disease. The proximity between the root apex and the cortical bone of the maxillary sinus using the linear measurement (mm) tool was used (RadiAnt, DICOM viewer, Poznan, Poland). The frequency found from maxillary sinus assessment was subjected to the Kolmogorov-Smirnov normality test, the Kruskal-Wallis test, the chi-square test with Bonferroni correction and a logistic regression analysis. A significance level of 5% was assumed. About 46.6% of the teeth showed a periapical pathology associated with an inflammatory reaction of the maxillary sinus. Among them, thickening of the maxillary sinus mucosa > 3 mm is the most common finding (59.3%). There was no difference between the types of maxillary sinus reactions and the variables studied (p >0.05). Periapical lesions on palatal roots were 2.17 times more likely to develop an inflammatory reaction than other roots (p < 0.05). Women were 2.04 times less likely to develop maxillary changes than men (p < 0.05). The distance between apex and floor and the presence or absence of endodontic treatment were not significantly associated with sinusitis. Periapical pathology could be related to maxillary inflammatory reaction of the sinus.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e245973"},"PeriodicalIF":0.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11653666/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Different Bleaching Techniques Using 6% Hydrogen Peroxide: Penetration Inside the Pulp Cavity, Bleaching Efficacy and Toxicity.

Brazilian dental journal Pub Date : 2024-12-06 eCollection Date: 2024-01-01 DOI: 10.1590/0103-644020245975

Gabrielle Gomes Centenaro, Michael Willian Favoreto, Taynara de Souza Carneiro, Michel Wendlinger, Christiane Philippini Ferreira, Lívia Câmara de Carvalho Galvão, Alessandro Dourado Loguercio

{"title":"The Effect of Different Bleaching Techniques Using 6% Hydrogen Peroxide: Penetration Inside the Pulp Cavity, Bleaching Efficacy and Toxicity.","authors":"Gabrielle Gomes Centenaro, Michael Willian Favoreto, Taynara de Souza Carneiro, Michel Wendlinger, Christiane Philippini Ferreira, Lívia Câmara de Carvalho Galvão, Alessandro Dourado Loguercio","doi":"10.1590/0103-644020245975","DOIUrl":"10.1590/0103-644020245975","url":null,"abstract":"This in vitro study aimed to quantify the penetration of hydrogen peroxide (HP), bleaching efficacy (BE) and toxicity in larvae in different bleaching techniques using 6% HP. Sixty maxillary premolars were divided in six groups (n = 10): Pola Luminate (PL), VivaStyle Paint On PIus (VS), PolaDay (PD), White Class (WC) and Whiteness HP Automixx (AM). A group not exposed to bleaching agents was evaluated as a control group (CG). Previously, the initial HP concentration in the bleaching agents was determined via titration and pH was measured with a digital pH meter. The concentration (µg/mL) of HP inside the pulp cavity was assessed using UV-Vis spectrophotometry. The BE (ΔE* ab and ΔE00) was evaluated with a digital spectrophotometer. The toxicity was evaluated in larvae model. Data from HP penetration and color change were evaluated for a one-way ANOVA and the Tukey's test, as well as Kaplan-Meier death curve for toxicity (α = 0.05). VS, PD and AM had an initial concentration lower than that the manufacturers reported. VS had a more acidic pH. PL and WC showed a significantly lower HP amount inside the pulp cavity when compared to VS (p = 0.001). Regarding BE, no significant difference was observed for ΔE* ab (p = 0.38), and ΔE00 (p = 0.42). No toxicity was observed when all 6% HP was compared to GC (p > 0.05). All 6% HP products tested showed bleaching efficacy, low penetration into the pulp chamber and were non-toxic to Tenebrio molitor larvae.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e245975"},"PeriodicalIF":0.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11653758/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial Effects of Cannabidiol (CBD)-infused Lozenges against Streptococcus mutans in Oral Health.

Brazilian dental journal Pub Date : 2024-12-06 eCollection Date: 2024-01-01 DOI: 10.1590/0103-644020245988

Jila Torabi, Henrique Pedro Soares Luis, Gohar Mkrtchyan, Shohreh Derakhshan Alavijeh, Sameen Dezfoli, Michelle Hurlbutt

{"title":"Antimicrobial Effects of Cannabidiol (CBD)-infused Lozenges against Streptococcus mutans in Oral Health.","authors":"Jila Torabi, Henrique Pedro Soares Luis, Gohar Mkrtchyan, Shohreh Derakhshan Alavijeh, Sameen Dezfoli, Michelle Hurlbutt","doi":"10.1590/0103-644020245988","DOIUrl":"10.1590/0103-644020245988","url":null,"abstract":"Cannabidiol presents several benefits, including but not limited to its analgesic, antioxidant, anti-inflammatory, antimicrobial, anti-pruritic, and anti-cancer properties. In this clinical trial, the antimicrobial impact of CBD-infused lozenges on Streptococcus mutans was examined using quantitative polymerized chain reaction (qPCR) bacterial analysis. This clinical trial involved 30 dental hygiene and nursing students who met the inclusion criteria participated in the study and were divided into two groups: experimental and control. The experimental group was given CBD-infused lozenges for 15 days, while the control group received sugar-free candy. Participants consumed one CBD-infused lozenge (300 mg) daily for 15 days, allowing it to dissolve slowly in the mouth for gradual absorption. The study focused on measuring changes in the salivary levels of Streptococcus mutans using quantitative polymerized chain reaction (qPCR) tests. Saliva samples were collected, and DNA extracted for qPCR analysis, assessing the bacterial load. The results, analyzed using a t-test, showed a significant decrease in Streptococcus mutans levels in the experimental group compared to the control group, with a statistically significant difference (p=0.0299).These findings suggest that cannabidiol may effectively reduce Streptococcus mutans in saliva, thus potentially helping to lower the risk of tooth decay as a multifactorial disease. This study underscores the potential of cannabidiol in enhancing oral health and calls for further research to explore its therapeutic applications in dental care.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e245988"},"PeriodicalIF":0.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11653757/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0