Brazilian dental journal最新文献_第2页

Effect of argon plasma and dentin rehydration on bond strength of dental adhesives to etched dentin. 氩等离子体和牙本质再水化对牙本质黏合剂黏结强度的影响。

Brazilian dental journal Pub Date : 2024-12-16 eCollection Date: 2024-01-01 DOI: 10.1590/0103-644020246029

Daniela Bandeira Dos Santos, Vitaliano Gomes de Araújo Neto, Amanda Endres Willers, Carolina Bosso André, Marcelo Giannini

{"title":"Effect of argon plasma and dentin rehydration on bond strength of dental adhesives to etched dentin.","authors":"Daniela Bandeira Dos Santos, Vitaliano Gomes de Araújo Neto, Amanda Endres Willers, Carolina Bosso André, Marcelo Giannini","doi":"10.1590/0103-644020246029","DOIUrl":"10.1590/0103-644020246029","url":null,"abstract":"This study investigated the effects of nonthermal atmospheric pressure plasma (NAPP) application and dentin rehydration with water (REHY) on bond strength (BS) of adhesives. Three etch-and-rinse adhesives were tested: Scotchbond Multi-Purpose (SBM / water-based primer + adhesive resin), Gluma Bond Universal (GBU / single-bottle containing acetone as organic solvent) and Prime&Bond Universal (PBU / single-bottle containing propanol as organic solvent). Adhesives were applied: 1- to phosphoric acid-etched dentin (Control), 2- after NAPP application for 45 seconds to etched dentin or 3- after REHY with water (10 seconds) of plasma-treated etched dentin. BS was performed using human molars and microtensile test, in which specimens were tested after 24 hours or one year (n=6). BS data were analyzed by three-way mixed ANOVA and Bonferroni's test (α=0.05). The morphology of etched dentin (n=3) and the dentin-adhesive interfaces (n=3) were evaluated using SEM. NAPP and REHY did not increase the BS of GBU and PBU. The BS of SBM to etched dentin not treated with NAPP (Control) reduced significantly after one year. The PBU adhesive showed the highest bond strength to dentin when the adhesives tested were applied after REHY. Phosphoric acid demineralized dentin to a depth of 5 mm that allowed the adhesive penetration into the dentinal tubules, forming the resin tags. Interfacial structures were maintained after one year. Argon plasma treatment was not beneficial in increasing the dentin bond strength of adhesives to etched-dentin at 24 hours. However, rehydration after plasma application prevented the bond strength reduction for three-step adhesive after one year.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e246029"},"PeriodicalIF":0.0,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11654011/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dimethyl sulfoxide affects the viability and mineralization activity of apical papilla cells in vitro. 二甲亚砜对体外培养的根尖乳头细胞的活力和矿化活性有影响。

Brazilian dental journal Pub Date : 2024-12-16 eCollection Date: 2024-01-01 DOI: 10.1590/0103-644020246054

Letícia Martins Santos, Danielle Yumi Shimabuko, Carla Renata Sipert

{"title":"Dimethyl sulfoxide affects the viability and mineralization activity of apical papilla cells in vitro.","authors":"Letícia Martins Santos, Danielle Yumi Shimabuko, Carla Renata Sipert","doi":"10.1590/0103-644020246054","DOIUrl":"10.1590/0103-644020246054","url":null,"abstract":"Dimethyl sulfoxide (DMSO) is widely used as an adjuvant in dissolving insoluble compounds in an aqueous medium; however, it can induce significant molecular changes in cells. The possible damages may occur obeying a tissue-specific profile, and the effect on human apical papilla cells (hAPC) remains unknown. Therefore, this study aimed to evaluate DMSO effects on the viability and mineralization activity in hAPC cultures in vitro and to establish standards of maximum concentrations for its use in laboratory routines. hAPCs were cultured, plated, and maintained in media containing increasing concentrations of Dimethyl sulfoxide (0.1%, 0.5%, 1%, 5%, and 10%) for 24 h, 48 h, 72 h, and 7 days. At each time point, the cells were subjected to the MTT assay. The Alizarin red S staining assay was performed to evaluate the osteo/odontogenic mineralization potential of hAPC DMSO-exposed (0.1%, 0.5%, and 1%) in the 21-day time-point. Statistical analysis was performed using one-way analysis of variance followed by Tukey's post hoc test (p<0.05). In general, the 5% and 10% DMSO concentrations were shown to be cytotoxic for hAPC at all analyzed time points, and the hAPC DMSO-stimulated presented higher osteo/odontogenic mineralization potential. Therefore, the 5% and 10% DMSO concentrations should be avoided, and the mineralization activity assay should be carefully designed in order to avoid biases at in vitro assays using hAPC cultures.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e246054"},"PeriodicalIF":0.0,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11654018/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of a pre-enlargement on fracture incidence of reused reciprocating instruments: A clinical study. 预扩对重复使用往复式器械骨折发生率的影响：一项临床研究。

Brazilian dental journal Pub Date : 2024-12-16 eCollection Date: 2024-01-01 DOI: 10.1590/0103-644020246147

Gustavo De-Deus, Guilherme Alves Palhares, Emmanuel João Nogueira Leal da Silva, Felipe Gonçalves Belladonna, Diogo da Silva Oliveira, Kilber Duarte Carvalho, Erick Miranda Souza, Marco Aurélio Versiani

{"title":"Effects of a pre-enlargement on fracture incidence of reused reciprocating instruments: A clinical study.","authors":"Gustavo De-Deus, Guilherme Alves Palhares, Emmanuel João Nogueira Leal da Silva, Felipe Gonçalves Belladonna, Diogo da Silva Oliveira, Kilber Duarte Carvalho, Erick Miranda Souza, Marco Aurélio Versiani","doi":"10.1590/0103-644020246147","DOIUrl":"10.1590/0103-644020246147","url":null,"abstract":"This study assessed the impact of pre-enlarging the root canal using a size 25 K-file on the fracture incidence of three reciprocating instruments after multiple uses. After a glide path performed with a size 15 K-file, the instruments (Reciproc R25, Reciproc Blue R25, and WaveOne Gold Primary) were initially used in 936 root canals. Then, for the second and third uses (933 and 927, respectively), a pre-enlargement using a size 25 K-file at the working length was done. Periapical radiographs assessed fragment location and length, and chi-square tests compared observed and expected frequencies (α = 5%). The fracture incidence during reuse ranged from 0% to 0.64%. Reciproc did not fracture during reuse (P = 0.99), but experienced two fractures in the third use (P = 0.000). Reciproc Blue and WaveOne Gold each had one fracture during the second (P = 0.000) and third (P = 0.000) uses. Following a pre-enlargement using a size 25 K-file for the second and third uses in 1,860 canals, only 6 instruments fractured (0.32%), suggesting that this approach may prolong the lifespan of these instruments and serve as an alternative for their reuse with a reduced risk of fracture.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e246147"},"PeriodicalIF":0.0,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11653994/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Global mapping of randomized controlled trials in dentistry. 牙科随机对照试验的全球制图。

Brazilian dental journal Pub Date : 2024-12-16 eCollection Date: 2024-01-01 DOI: 10.1590/0103-644020246233

Mayara Colpo Prado, Lara Dotto, Bernardo Agostini, Rafael Sarkis-Onofre

{"title":"Global mapping of randomized controlled trials in dentistry.","authors":"Mayara Colpo Prado, Lara Dotto, Bernardo Agostini, Rafael Sarkis-Onofre","doi":"10.1590/0103-644020246233","DOIUrl":"10.1590/0103-644020246233","url":null,"abstract":"This meta-research sought to evaluate the conduct, reporting, and main characteristics of published randomized controlled trials (RCTs) in dentistry. A search was performed on PubMed for RCTs in dentistry indexed from 31 December 2016 to 31 December 2021. Two reviewers independently screened the studies for the presence of eligibility criteria. Only studies in English were considered. Journal and author data, subject, citation and publishing metrics, reporting, and details of study conduct were collected. A descriptive analysis of the data, a map depicting the number of RCTs per country, and a network graph of scientific collaboration among different countries were presented. We included 844 articles. The main research area was periodontics (16.35%). The highest number of RCTs was attributed to Brazil (16.59%). Authors from the USA established the most links with other countries. Most studies did not report the use of CONSORT (67.89%), the type of randomization (69.31%), or the mechanism used to implement the random allocation sequence (52.37%). However, most studies included \"randomized\" in the title (71.92%) and reported the method for generating a random allocation sequence (65.88%) and blinding (74.88%). RCTs are the basis for clinical decision-making. Our results provide a better view of current RCTs and identify areas that require improvement. Brazil was the country that produced the most RCTs, and the USA was the main collaborator. We emphasize the variability of reporting characteristics and study conduct.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e246233"},"PeriodicalIF":0.0,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11654338/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-surgical endodontic retreatments in Brazil's public dental services: a 15-year nationwide register-based study. 巴西公共牙科服务的非手术牙髓再治疗：一项为期15年的全国性登记研究。

Brazilian dental journal Pub Date : 2024-12-16 eCollection Date: 2024-01-01 DOI: 10.1590/0103-644020246004

Guibson da Silva Litaiff, Ricardo Barbosa Lima, Aluísio Eustáquio de Freitas Miranda-Filho, Paulo Nelson-Filho, Raquel Assed Bezerra da Silva, Léa Assed Bezerra da Silva

{"title":"Non-surgical endodontic retreatments in Brazil's public dental services: a 15-year nationwide register-based study.","authors":"Guibson da Silva Litaiff, Ricardo Barbosa Lima, Aluísio Eustáquio de Freitas Miranda-Filho, Paulo Nelson-Filho, Raquel Assed Bezerra da Silva, Léa Assed Bezerra da Silva","doi":"10.1590/0103-644020246004","DOIUrl":"10.1590/0103-644020246004","url":null,"abstract":"The objective of this study was to analyze the provision of non-surgical endodontic retreatments in Brazil's public dental services from 2008 to 2022. A time series was outlined for this purpose. The annual numbers of non-surgical endodontic retreatments were retrieved from the Brazilian National Outpatient Information System and adjusted per 100,000 inhabitants to obtain the incidence between 2008 and 2022. Comparison of the incidence between types of teeth, temporal trend estimation, and evaluation of the COVID-19 pandemic-related impacts were conducted with a significance level of 5%. In this timeframe, 1,270,182 non-surgical endodontic retreatments were carried out. A higher incidence was observed among single-rooted teeth (328/100,000) when compared to double-rooted (183/100,000) and multi-rooted teeth (112/100,000) (p <0.05). The annual incidences showed a significantly decreasing trend over the last 15 years for all types of teeth (p <0.05), as well as demonstrated a highly correlated pattern of temporal variation (p <0.05). In addition, there was no influence on this outcome when removing the period between 2020 and 2022 (all temporal trends remained decreasing). However, the monthly incidence was significantly lower in the first, second, and third years after the COVID-19 pandemic onset (p <0.05). The provision of non-surgical endodontic retreatments in Brazil's public dental services has experienced a dramatic decline over the past 15 years, including after the COVID-19 pandemic.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e246004"},"PeriodicalIF":0.0,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11653991/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological Properties of Bioceramic Sealers on Osteoblastic Cells: A Comparative Study. 生物陶瓷封口处成骨细胞生物学特性的比较研究。

Brazilian dental journal Pub Date : 2024-12-16 eCollection Date: 2024-01-01 DOI: 10.1590/0103-6440202406037

Angelita Piovezana Guerra, Danielle Gregorio, Gean Carlos Yamamoto, Nathalia Thalitha Bernardes Dos Santos, Regina Celia Poli-Frederico, Luciana Prado Maia

{"title":"Biological Properties of Bioceramic Sealers on Osteoblastic Cells: A Comparative Study.","authors":"Angelita Piovezana Guerra, Danielle Gregorio, Gean Carlos Yamamoto, Nathalia Thalitha Bernardes Dos Santos, Regina Celia Poli-Frederico, Luciana Prado Maia","doi":"10.1590/0103-6440202406037","DOIUrl":"10.1590/0103-6440202406037","url":null,"abstract":"This study aimed to assess the biological properties of two ready-to-use bioceramic sealers (EndoSequence BC Sealer - EBCS; Bio-C Sealer - BCS) on osteoblastic lineage cells. MC3T3 osteoblast-like cells were exposed to extracts of bioceramic materials. Cytotoxicity was evaluated using the MTT method, genotoxicity was assessed by the micronucleus test and the expression of BMP1, BMP2 and ALP was measured by RT-qPCR, after 1, 3 and 7 days. Wound healing was monitored at 1, 2 and 3-day intervals using the scratch test. Statistical analysis involved a two-factor ANOVA, followed by the Tukey Test for multiple comparisons (α=0.05). Cytotoxicity assessment revealed no significant differences between the materials and the control group at any of the time points, indicating that neither material exhibited cytotoxic effects. However, both cements induced greater micronuclei formation compared to the control on days 1 and 7 (p<0.05) when evaluating genotoxicity. No significant differences between the groups were observed in wound healing at any of the time intervals. Both biomaterials upregulated the expression of BMP1, BMP2 and ALP. The two bioceramic sealers exhibited comparable biological properties, including cytocompatibility, promotion of wound healing, and upregulation of bone formation-related genes in osteoblast-like cells. While these results suggest the potential for safe clinical application, the observed genotoxicity warrants caution. Therefore, while the null hypothesis-that the two bioceramic sealers exhibit analogous properties-was accepted in terms of cytocompatibility and gene expression, further investigation is essential to fully ensure their safety and efficacy in bone-related procedures.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e246037"},"PeriodicalIF":0.0,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11654336/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142856748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Does the pH of the grape seed extract interfere in the biomodification capacity of dentin collagen? 葡萄籽提取物的pH值是否会干扰牙本质胶原蛋白的生物修饰能力？

Brazilian dental journal Pub Date : 2024-12-16 eCollection Date: 2024-01-01 DOI: 10.1590/0103-6440202406048

Marcelo Victor Sidou Lemos, Gabriela Araújo Lourenço, Samuel Chillavert Dias Pascoal, Talita Arrais Daniel Mendes, Sérgio Lima Santiago

{"title":"Does the pH of the grape seed extract interfere in the biomodification capacity of dentin collagen?","authors":"Marcelo Victor Sidou Lemos, Gabriela Araújo Lourenço, Samuel Chillavert Dias Pascoal, Talita Arrais Daniel Mendes, Sérgio Lima Santiago","doi":"10.1590/0103-6440202406048","DOIUrl":"10.1590/0103-6440202406048","url":null,"abstract":"The aim of this study was to verify the biomodifying action of 6.5% grape seed extract solutions, with different pH, when applied on dentin collagen. Dentin bars (1.7 mm x 6.0 mm x 0.5 mm) were demineralized for 5 hours in 10% phosphoric acid, and distributed into the following groups: acid solution (pH=4.42); neutral (pH=6.96); alkaline (pH=11.92) and distilled water (pH=6.75). Three-point flexural test (n=10) and mass variation (n=10) were assessed at different periods (baseline, after biomodification, 7 and 14 days of remineralizing solution storage). For qualitative analysis, similar dentin bars were prepared and analyzed using Fourier transform infrared spectroscopy (FT-IR) and Raman Spectroscopy (FT-Raman) before and after 1 hour of immersion in biomodifying solution with different pH. Data were subjected to Shapiro-Wilk normality tests, followed by two-way ANOVA for repeated measures and Tukey's post-test (p<0.05). Alkaline solution was effective in increasing the modulus of elasticity, showing a decrease after 7 days and subsequent stabilization after 14 days of storage. Acid solution group showed a greater increase in the modulus of elasticity immediately after biomodification, but it was not stable over storage. Regarding mass variation, only the acid solution showed an increase in mass after biomodification and 14 days of storage. In relation to FT-IR and FT-Raman, all solutions showed interaction with collagen at some level. Therefore, pH of the solution directly influences the action of the grape seed extract, with satisfactory results being found in both acidic and alkaline solutions.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e246048"},"PeriodicalIF":0.0,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11654020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optical properties and color stability of a translucent zirconia: effects of finishing after grinding. 一种半透明氧化锆的光学性质和颜色稳定性：研磨后精加工的影响。

Brazilian dental journal Pub Date : 2024-12-16 eCollection Date: 2024-01-01 DOI: 10.1590/0103-6440202406094

Pablo Machado Soares, Camila Pauleski Zucuni, Bruna Dias Ilha, Leticia Borges Jacques, Liliana Gressler May, Luiz Felipe Valandro

{"title":"Optical properties and color stability of a translucent zirconia: effects of finishing after grinding.","authors":"Pablo Machado Soares, Camila Pauleski Zucuni, Bruna Dias Ilha, Leticia Borges Jacques, Liliana Gressler May, Luiz Felipe Valandro","doi":"10.1590/0103-6440202406094","DOIUrl":"10.1590/0103-6440202406094","url":null,"abstract":"This study evaluated the effect of grinding and finishing treatments (polishing or glaze) on the color stability, translucency and opalescence of a translucent zirconia (3Y-TZP) after immersion in red wine. Discs (Ø= 12 mm; thickness 0.8 mm) of 3Y-TZP (Zenostar T, Ivoclar AG) were randomly allocated (n= 8) according to the surface treatment factor: Ctrl - as-sintered; Gr - grinding with diamond bur #4219; Gr + Pol - grinding followed by polishing; Gr + Gl - grinding followed by glaze. Surface roughness and scanning electron microscopy were carried out. All specimens were subjected to daily immersions in red wine for 30 minutes during 18 days. The color differences (ΔE00) were measured prior, after 9 and 18 days of immersion through the CIEDE2000 formula, as same as translucency (TP00) and opalescence (OP) parameters. Kruskal Wallis, repeated measures ANOVA/Tukey, and Spearman's correlation tests were performed to evaluate the roughness, optical and correlation data, respectively. The Gr + Gl depicted the lowest ΔE00 value after 9 days. The Gr group showed the greatest ΔE00 values in all immersion times, whereas the other groups presented similar behavior after 18 days. The Gr and Gr + Pol groups had the highest Ra and Rz values. The correlation between roughness and color change was moderate and significant (p= 0.02, r= 0.42). No differences were observed for TP00 and OP. The surface treatments affected the roughness and color stability of zirconia, being glaze application more effective to reduce color alterations after the immersion protocol.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e246094"},"PeriodicalIF":0.0,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11653993/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Repopulation of a 3D simulated periapical lesion cavity with dental pulp stem cell spheroids with triggered osteoblastic differentiation. 引发成骨细胞分化的牙髓干细胞球体三维模拟根尖周围病变腔的重建。

Brazilian dental journal Pub Date : 2024-12-16 eCollection Date: 2024-01-01 DOI: 10.1590/0103-644020235847

Vítor Luís Ribeiro, Janaína A Dernowsek, Roger R Fernandes, Dimitrius L Pitol, João Paulo Mardegan Issa, Jardel F Mazzi-Chaves, Karina Fittipaldi Bombonato-Prado, Manoel Damião Sousa-Neto, Geraldo Aleixo Passos

{"title":"Repopulation of a 3D simulated periapical lesion cavity with dental pulp stem cell spheroids with triggered osteoblastic differentiation.","authors":"Vítor Luís Ribeiro, Janaína A Dernowsek, Roger R Fernandes, Dimitrius L Pitol, João Paulo Mardegan Issa, Jardel F Mazzi-Chaves, Karina Fittipaldi Bombonato-Prado, Manoel Damião Sousa-Neto, Geraldo Aleixo Passos","doi":"10.1590/0103-644020235847","DOIUrl":"10.1590/0103-644020235847","url":null,"abstract":"We established a proof-of-concept model system for the biological healing of periapical lesions using stem cell spheroids. Mesenchymal stem cells from human exfoliated deciduous teeth (SHED) were cultured in a 2D monolayer and then as 3D multicellular spheroids. An image of a periapical lesion of an upper lateral incisor tooth was obtained by computed tomography and was used as a model for photopolymer resin 3D printing to generate a negative frame of the lesion. The negative model served to prepare a positive model of the periapical lesion cavity in an agarose gel. SHED that were cultured in monolayers or as spheroids were seeded in the positive lesion mold before or after osteoblastic differentiation. The results showed that compared to cells cultured in monolayers, spheroids exhibited uniform cellularity and a greater viability within the lesion cavity, which was accompanied by a temporal reduction in the expression of CD13, CD29, CD44, CD73, and CD90 mRNAs that are typically expressed by stem cells. Concomitantly, the expression of markers that characterize osteoblastic differentiation (RUNX2, ALP, and BGLAP) increased. These results provide a new perspective for regenerative endodontics with the use of SHED-derived spheroids to repair periapical lesions.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e235847"},"PeriodicalIF":0.0,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11654009/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rho Kinases and Reactive Oxygen Species in Autophagy Regulation by Pressure in Periodontal Ligament Cells. Rho激酶和活性氧在牙周膜细胞压力自噬调节中的作用。

Brazilian dental journal Pub Date : 2024-12-06 eCollection Date: 2024-01-01 DOI: 10.1590/0103-6440202405944

Miriam Hardt, Alexandra Mayr, Eric Kutschera, Jana Marciniak, Erika Calvano Küchler, Christian Kirschneck, James Deschner, Andreas Jäger, Svenja Beisel-Memmert

{"title":"Rho Kinases and Reactive Oxygen Species in Autophagy Regulation by Pressure in Periodontal Ligament Cells.","authors":"Miriam Hardt, Alexandra Mayr, Eric Kutschera, Jana Marciniak, Erika Calvano Küchler, Christian Kirschneck, James Deschner, Andreas Jäger, Svenja Beisel-Memmert","doi":"10.1590/0103-6440202405944","DOIUrl":"10.1590/0103-6440202405944","url":null,"abstract":"Autophagy is a self-digestion mechanism of cells, which is related to cell stress. It enables cell survival by maintaining cellular homeostasis or initiates cell death. This study aimed to investigate the intracellular signaling of pressure-induced autophagy regulation in human periodontal ligament (PDL) cells and to analyze the involvement of Rho kinases (ROCK) and reactive oxygen species (ROS) in particular. Human PDL cells were treated with the ROCK inhibitor Y-27632 and the ROS scavenger N-acetylcysteine (NAC) in combination with pressure magnitudes of 2, 6, and 8 g/cm2 over 16 hours. Cells treated with rapamycin served as a positive control and untreated cells as a control group. The Cyto-ID® Autophagy Detection Kit was used for flow cytometric analysis. Statistical analysis was performed using ANOVA and post-hoc tests. The results show that the pressure-induced autophagy was affected differently by the two inhibitors (p<0.05). The application of Y-27632 led to a significant reduction in autophagy in all pressure groups. The application of NAC led to reduced autophagy at pressures of 2 g/cm2 and 6 g/cm2. At 8 g/cm2, this effect was no longer present. In the control group, autophagy was significantly reduced by Y-27632 and significantly increased by NAC. Our data suggest that both Rho-kinase and reactive oxygen species could influence pressure-induced autophagy regulation in PDL cells.","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e245944"},"PeriodicalIF":0.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11653791/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143712698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0