Brazilian dental journal最新文献

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Evaluation the stress distribution in root canals by 3D finite element analysis after the materials used in reattaching the vertically root fractured fragments. 通过三维有限元分析,在使用材料重新连接垂直根折的碎片后,评估根管中的应力分布。
Brazilian dental journal Pub Date : 2024-03-22 eCollection Date: 2024-01-01 DOI: 10.1590/0103-6440202405833
Senem Yiğit Özer, Salih Danişman, Törem Özer
{"title":"Evaluation the stress distribution in root canals by 3D finite element analysis after the materials used in reattaching the vertically root fractured fragments.","authors":"Senem Yiğit Özer, Salih Danişman, Törem Özer","doi":"10.1590/0103-6440202405833","DOIUrl":"10.1590/0103-6440202405833","url":null,"abstract":"<p><p>The aim was to evaluate the effect of stress distribution on vertical, horizontal, and oblique forces on the tooth model after reattaching the fragments of the maxillary incisor with vertical root fracture (VRF) using different materials, by 3D finite element analysis (FEA). Tooth with a root canal, spongious, and cortical bone models were designed. VRF was modeled on a tooth with 4 different re-attachment models: Group 1: dual-cure cement (DC)+fiber reinforced composite (FRC), Group 2: DC+polyethylene fiber, Group 3: DC+glass fiber, and Group 4:DC. 100 N force was applied in 3 different directions. Maximum principal stresses (σmax) of dentin, and re-attachment materials were evaluated on colored images. The highest σmax values ​​were on the repair materials under vertical forces for Groups 1 and 4, respectively; Groups 2 and 3 showed similarity. The highest σmax values in repair materials under horizontal and oblique forces were observed in Group 3 however the lowest σmax values in repair materials under oblique and horizontal forces were observed in Group 1. The stress values ​​on repair materials gradually increased respectively starting from horizontal to vertical. As the elasticity modulus of the repair materials increased, the stress values ​​on root dentin increased. Through all force directions, except vertical forces, lower stress values were observed with FRC. The fracture resistance was bigger when using solely FRC or dual-cure resin cement in comparison to fiber-supported designs. Adding polyethylene fiber to re-restorations decreased stress values ​​compared to glass fiber addition. Therefore, when adding fibers, polyethylene fiber will be advantageous.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e245833"},"PeriodicalIF":0.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10976308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140308464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Ceramic Thickness on the Bond Strength to Resin-Luting Agents before and after Thermal Cycling. 陶瓷厚度对热循环前后树脂洗脱剂粘接强度的影响
Brazilian dental journal Pub Date : 2024-03-22 eCollection Date: 2024-01-01 DOI: 10.1590/0103-6440202405619
Carolina Rodrigues de Souza, Ana Rosa Costa, Lincoln Pires Silva Borges, Analia Gabriella Borges Ferraz, Rafael Leonardo Xediek Consani, Rafael Rocha Pacheco, Américo Bortolazzo Correr, Lourenço Correr-Sobrinho
{"title":"Effect of Ceramic Thickness on the Bond Strength to Resin-Luting Agents before and after Thermal Cycling.","authors":"Carolina Rodrigues de Souza, Ana Rosa Costa, Lincoln Pires Silva Borges, Analia Gabriella Borges Ferraz, Rafael Leonardo Xediek Consani, Rafael Rocha Pacheco, Américo Bortolazzo Correr, Lourenço Correr-Sobrinho","doi":"10.1590/0103-6440202405619","DOIUrl":"10.1590/0103-6440202405619","url":null,"abstract":"<p><p>This study investigated microshear bond strength (µSBS) of two (2) dual-cured resin-luting agents (RelyX™ Ultimate and RelyX™ U200) when photoactivated through varying thicknesses of lithium disilicate, with or without thermal cycling. Discs of IPS e.max Press of 0.5, 1.5, and 2 mm in thickness were obtained. Elastomer molds (3.0 mm in thickness) with four cylinder-shaped orifices 1.0 mm in diameter, were placed onto the ceramic surfaces and filled with resin-luting agents. A Mylar strip, glass plate, and load of 250 grams were placed over the filled mold. The load was removed and the resin-luting agents were photoactivated through the ceramics using a single-peak LED (Radii Plus.) All samples were stored in distilled water at 37oC for 24 h. Half of the samples were subjected to thermal cycling (3,000 cycles; 5ºC and 55ºC). All samples were then submitted to µSBS test using a universal testing machine (Instron 4411) at a crosshead speed of 0.5 mm/min. Data were submitted to three-way ANOVA and Tukey post-hoc test (α=0.05). The mean µSBS at 24 h was significantly higher than after thermal cycling (p<0.05). No statistical difference was found between resin-luting agents (p > 0.05). The mean µSBS for groups photoactivated through 0.5 mm ceramic were significantly higher than 1.5 mm and 2.0 mm (p < 0.05). In conclusion, increased ceramic thicknesses reduced the bond strength of tested resin-luting agents to lithium disilicate. No differences were found between resin-luting agents. Thermal cycling reduced the bond strength of both resin-luting agents.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e245619"},"PeriodicalIF":0.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10976309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140308477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of Access Cavity Design on Fracture Resistance of Endodontically Treated Maxillary First Premolar: In Vitro. 牙髓治疗后上颌第一前磨牙通路腔设计对抗折性的影响:体外试验
Brazilian dental journal Pub Date : 2024-03-22 eCollection Date: 2024-01-01 DOI: 10.1590/0103-6440202405676
Anju Daniel, Abdul Rahman Saleh, Anas Al-Jadaa, Waad Kheder
{"title":"Impact of Access Cavity Design on Fracture Resistance of Endodontically Treated Maxillary First Premolar: In Vitro.","authors":"Anju Daniel, Abdul Rahman Saleh, Anas Al-Jadaa, Waad Kheder","doi":"10.1590/0103-6440202405676","DOIUrl":"10.1590/0103-6440202405676","url":null,"abstract":"<p><p>This study was designed to investigate the impact of access cavity designs on fracture resistance of endodontically treated maxillary first premolars. The study sample consisted of 72 intact maxillary first premolars, randomly divided into six groups (n = 12). A standardized proximal cavity preparation was prepared for all samples using standard bur. Groups I: control group with only standard proximal cavity and no endodontic access, group II: Truss access cavity, group III: Separated access to buccal and palatal canals without removal of dentine in between, group IV: Access to buccal and palatal canals with removal of dentine in between, group V: Traditional access cavity, group VI: Mesio-occlusal-distal cavity (MOD). For groups I and VI, only composite restoration was used to restore the proximal cavity, while for groups II- V, the access was prepared and endodontic treatment was performed on all teeth, then composite restoration was placed. The root canals were instrumented using nickel-titanium files, irrigated with sodium hypochlorite, and filled with AH plus sealer and gutta-percha using warm vertical condensation. All samples were then placed in an acrylic mold and underwent thermal aging for 10,000 cycles between 5 and 55°C. The samples were fixed in a universal testing machine with the long axis of the roots positioned at 20° to a load applied at a crosshead speed of 1 mm/min using a stainless steel semi-spherical indenter (Ø = 3 mm) until fracture occurred to determine the fracture resistance force in Newton. The normality test (Shapiro-Wilk) showed that data are normally distributed. Group II exhibited the highest mean fracture resistance, and group VI was the least likely to resist the fracture. No statistically significant differences between tested groups (p-value = 0.237). The MOD group showed a more unfavorable mode of fracture compared to other groups. No significant difference in fracture resistance between conservative and traditional access cavities. The missing marginal ridges, such as in MOD cavities played an important role in decreasing the fracture resistance of endodontically treated teeth.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e245676"},"PeriodicalIF":0.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10976331/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140308465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preheated acid associated with silane and electric current in the adhesion of the resin cement to ceramic. 在树脂水泥与陶瓷的粘合过程中,预热的酸与硅烷和电流相关联。
Brazilian dental journal Pub Date : 2024-03-22 eCollection Date: 2024-01-01 DOI: 10.1590/0103-6440202405641
Gustavo Belmiro Casaburi, Marcos Henrique Ramos da Silva, Lourenço Correr-Sobrinho, Rafael Leonardo Xediek Consani
{"title":"Preheated acid associated with silane and electric current in the adhesion of the resin cement to ceramic.","authors":"Gustavo Belmiro Casaburi, Marcos Henrique Ramos da Silva, Lourenço Correr-Sobrinho, Rafael Leonardo Xediek Consani","doi":"10.1590/0103-6440202405641","DOIUrl":"10.1590/0103-6440202405641","url":null,"abstract":"<p><p>This study verified the effect of the combination of preheated hydrofluoric acid/silane/electric current in the adhesion of the resin cement to ceramic. IPS E.max Press ceramic discs embedded in PVC rigid tubes were divided into four groups associating preheated hydrofluoric acid and silane applied with electrical current (n=10): Ha+S (Heated acid + silane); Ha+S+Ec (Heated acid + silane + electrical current); A+S (Acid + silane) and A+S+Ec (Acid + silano + electrical current). Resin cement/ceramic samples were stored in water at 37°C for 24h. After storage, they were submitted to the microshear test, fracture analysis, and contact angle at 24h or after thermocycling (10,000 cycles/5-55ºC). Bond strength data were evaluated by two-way ANOVA. For comparison between evaluation times (24h or thermocycling) was applied unpaired t-test. A significance post-hoc test of p=0.05 was assumed for analyses and graphs (GraphPad Prism 9.0 software). At 24h, the microshear strength showed similar values between Ha+S, Ha+S+Ec, and A+S+Ec groups, while A+S showed the lowest value with a statistical difference. After thermocycling, Ha+S and Ha+S+Ec were similar, as well as A+S and A+S+Ec. There was a significant difference in all groups comparing 24h (highest value) with after thermocycling (lowest value). Adhesive fracture was predominant in all groups and evaluation times. Ha+S and A+S groups showed higher contact angle values compared to the Ha+S+Ec and A+S+Ec with lower values. In conclusion, the association of preheated hydrofluoric acid/silane applied or not with electric current promoted different microshear strength values, fracture types, and contact angles in the resin cement/ceramic bond.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e245641"},"PeriodicalIF":0.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10976297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140308468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessing peri-implant bone microarchitecture: conventional vs. osseodensification drilling - ex vivo analysis. 评估种植体周围骨的微观结构:传统钻孔与骨增生钻孔--体外分析。
Brazilian dental journal Pub Date : 2024-03-22 eCollection Date: 2024-01-01 DOI: 10.1590/0103-6440202405599
Breno Fortes Bittar, Bruno Salles Sotto-Maior, Karina Lopes Devito, Gustavo Davi Rabelo, Alessandra Silveira Machado, Ricardo Tadeu Lopes, Neuza Maria Souza Picorelli Assis
{"title":"Assessing peri-implant bone microarchitecture: conventional vs. osseodensification drilling - ex vivo analysis.","authors":"Breno Fortes Bittar, Bruno Salles Sotto-Maior, Karina Lopes Devito, Gustavo Davi Rabelo, Alessandra Silveira Machado, Ricardo Tadeu Lopes, Neuza Maria Souza Picorelli Assis","doi":"10.1590/0103-6440202405599","DOIUrl":"10.1590/0103-6440202405599","url":null,"abstract":"<p><p>The aim was to evaluate primary implant stability and bone microarchitecture in two drilling situations, by comparing the conventional technique (CT) and osseodensification (OD) (Versah Burs - Jackson - Mississippi - USA). The implant insertion torque (IT), implant stability quotient (ISQ), and the peri-implant trabecular microstructure were assessed on bone fragments obtained from pig's tibia (n=12), divided between CT (n=6) and OD (n=6). After the drilling procedure, the implants were installed (3.5x8.5 mm, Epikut - SIN - São Paulo - Brazil). The IT and ISQ were measured using a digital torque wrench and resonance frequency analysis. Then, the bone fragments containing the implants were removed with a trephine and analyzed by Microtomography (µCT, 8.0 µm). The comparison between groups was performed using the unpaired t-test (α=0.05). The results revealed that OD promotes a higher insertion torque (CT: 7.67±2.44 Ncm; OD: 19.78±5.26 Ncm) (p=0.0005), although ISQ was not different (CT: 61.33±4.66; OD: 63.25±4.58) (p=0.48). There was a significant increase in peri-implant bone volume (CT: 23.17±3.39 mm3; OD: 32.01±5.75 mm3) (p=0.008), and trabecular parameters: separation (CT: 0.4357±0.03 mm; OD: 0.3865±0.04 mm) (p=0.0449), number (CT: 1.626±0.18 1/mm; OD: 1.946±0.13 1/mm) (p=0.007), and thickness (CT: 0.1130±0.009 mm; OD: 0.1328±0.015 mm) (p=0.02). Structure model index (SMI) data demonstrate no significant differences between groups (CT: 1.7±0.2; OD: 1.4±0.4) (p=0.12). In conclusion, OD increases the insertion torque values and promotes beneficial changes regarding bone microarchitecture compared with CT, revealing more peri-implant bone volume with consequent higher primary stability.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e245599"},"PeriodicalIF":0.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10976315/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140308474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative study of physical-chemical properties of bioactive glass ionomer cement. 生物活性玻璃离聚物水泥的物理化学特性比较研究。
Brazilian dental journal Pub Date : 2024-03-22 eCollection Date: 2024-01-01 DOI: 10.1590/0103-6440202405728
Nádia Buzignani Pires Ramos, Klissia Romero Felizardo, Sandrine Bittencourt Berger, Ricardo Danil Guiraldo, Murilo Baena Lopes
{"title":"Comparative study of physical-chemical properties of bioactive glass ionomer cement.","authors":"Nádia Buzignani Pires Ramos, Klissia Romero Felizardo, Sandrine Bittencourt Berger, Ricardo Danil Guiraldo, Murilo Baena Lopes","doi":"10.1590/0103-6440202405728","DOIUrl":"10.1590/0103-6440202405728","url":null,"abstract":"<p><p>This study analyzed the physical-chemical properties of bioactive ionomer materials. Cention N bioactive materials were evaluated chemically activated (CN) and light-cured (CN-LC), Equia Forte Fill (EQUI); conventional resin composite Filtek Z350 XT (Z350); resin glass ionomer cement Riva light Cure (RIVA) and flowable resin composite Filtek Bulk Fill Flow (BULK-F) were evaluated. Sixty specimens (n=10) were prepared for sorption (SR), solubility (SL), flexural strength (FS), shrinkage stress (SS), conversion degree (CD), microhardness (MI), and surface roughness (SR) tests. Non-cured and light-cured materials were assessed on FTIR. 30 human molar teeth were used in the bond strength test (BS). Data were subjected to ANOVA and post-hoc Tukey's test (5% of significance). EQUI showed more sorption in SR and no statistical difference from RIVA and CN-LC. CN group showed more solubility and EQUI presented less (p<0.05). BULK-F showed higher FS (MPa), without differences from CN and Z350, whereas EQUI presented the lowest FS not differing from RIVA. BULK-F and CN-LC showed more shrinkage stress differing from EQUI. CN-LC and CN showed higher CD differing from the other which showed no differences (p>0.05) between them. EQUI showed the highest hardness (p<0.05) in MI. There were no differences (p>0.05) in SR (µm). Z350 and BULK-F presented higher BS, whereas CN-LC showed the lowest, although not differing from EQUI and RIVA. Equia Forte's solubility and microhardness make it a good alternative as a restorative material. Cention N degree of conversion and flexural strength making it an esthetic option to amalgam.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e245728"},"PeriodicalIF":0.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10976313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140308476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Surface treatments of the zirconia-reinforced lithium disilicate ceramic in the adhesion to the resin cement. 氧化锆增强二硅酸锂陶瓷的表面处理对树脂胶结物粘附性的影响。
Brazilian dental journal Pub Date : 2024-03-22 eCollection Date: 2024-01-01 DOI: 10.1590/0103-6440202405674
Mirko A R Aguilera, Américo C Bortolazzo, Lourenço Correr-Sobrinho, Rafael L X Consani
{"title":"Surface treatments of the zirconia-reinforced lithium disilicate ceramic in the adhesion to the resin cement.","authors":"Mirko A R Aguilera, Américo C Bortolazzo, Lourenço Correr-Sobrinho, Rafael L X Consani","doi":"10.1590/0103-6440202405674","DOIUrl":"10.1590/0103-6440202405674","url":null,"abstract":"<p><p>This study verified the effect of surface treatments of the zirconia-reinforced lithium disilicate ceramic bonded to resin cement. Ceramic blocks were divided according to treatments (n=10): FA+SRX (Fluoric acid + silane RX), FA+MDP (Fluoric acid + MDP), FA+SCF+MDP (Fluoric acid + silane CF + MDP), FA+MEP (Fluoric acid + MEP), and MEP (Self-etch primer). Resin cement cylinders were made in the ceramic blocks, photoactivated with 1,200 mW/cm² for 40s, stored in water at 37°C for 24h, and evaluated by the microshear strength test, optical failure descriptive analysis (%), surface characterization (SEM) and contact angle (Goniometer). Other samples were submitted to 10,000 thermocycles between 5°C and 55°C. Bond strength data were submitted to two-way ANOVA and Tukey's test. Contact angle to one-way ANOVA and Games-Howell's test (5%). At 24h, MEP showed higher bond strength, and FA+SRX the lower. FA+MDP and FA+SCF+MDP showed similar values and FA+MEP was intermediate. After thermocycling, FA+SCF+MDP, FA+MEP, and MEP showed higher values, and FA+SRX the lower while FA+MDP was intermediate. When the periods were compared, FA+MDP, FA+SCF+MDP, FA+MEP, and MEP showed higher values for 24h while FA+SRX was similar. SEM showed retentive surface and crystal exposure when treated with FA+SCF+MDP. The less retentive surface was obtained with MEP, and the other treatments promoted intermediate irregularities. In conclusion, surface treatment and thermocycling promoted different values of adhesive strength and contact angle in a zirconia-reinforced lithium silicate ceramic. Failures were predominantly adhesive, and the ceramic surface was characterized by different levels of roughness and selective exposure of crystals.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e245674"},"PeriodicalIF":0.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10976312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140308480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of EGCG-based paste as intracanal dressing, in MMPs 2 and 9 expression in dog's periapical lesions. 将基于 EGCG 的糊剂作为根管内敷料对狗根尖周病变中 MMPs 2 和 9 表达的影响。
Brazilian dental journal Pub Date : 2024-03-22 eCollection Date: 2024-01-01 DOI: 10.1590/0103-6440202405509
Andiara De Rossi, Tadeu Pradela, Fernanda Souza Liévana, Raquel Assed Bezerra Segato, Jorge Esquiche León, Léa Assed Bezerra da Silva, Paulo Nelson-Filho
{"title":"Effect of EGCG-based paste as intracanal dressing, in MMPs 2 and 9 expression in dog's periapical lesions.","authors":"Andiara De Rossi, Tadeu Pradela, Fernanda Souza Liévana, Raquel Assed Bezerra Segato, Jorge Esquiche León, Léa Assed Bezerra da Silva, Paulo Nelson-Filho","doi":"10.1590/0103-6440202405509","DOIUrl":"10.1590/0103-6440202405509","url":null,"abstract":"<p><p>High expression of MMP-2 and MMP-9 in periapical lesions plays an important role in the degradation of the extracellular matrix. This study aimed to investigate the effect of epigallocatechin-3-gallate (EGCG)-based endodontic paste as an intracanal dressing on the expression of MMP-2 and MMP-9 in periapical lesions. Periapical lesions were experimentally induced in 35 mature beagle dog premolars randomly divided into healthy teeth, untreated periapical lesions, periapical lesions treated in a single session (control groups), and periapical lesions treated in two sessions with EGCG or calcium hydroxide-based pastes (experimental groups). After 120 days, specimens were obtained for histopathologic and immunofluorescence analyses to assess the expression of MMP-2 and MMP-9. The statistical analysis was performed using a p-value of 0.05. Endodontic treatment in two sessions using medication with EGCG and calcium hydroxide-based pastes provided similar repair of the apical and periapical tissues and neoformation of periodontal ligament fibers, cementum, and alveolar bone (p>0.05). The experimental groups treated in two sessions with both medications presented expression of MMP-2 and MMP-9 similar to that in healthy teeth (p>0.05), and significantly lower than teeth treated in a single session or untreated periapical lesions (p <0.001). Expression of MMP-2 and MMP-9 was observed in the cytoplasm of fibroblasts, osteoblasts, cementoblasts, cementocytes, and vascular endothelium. The use of EGCG-based endodontic paste reduced the expression of MMP-2 and MMP-9 and allowed repair of periapical lesions, similar to calcium hydroxide-based paste, and superior to treatment performed in a single session.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e245509"},"PeriodicalIF":0.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10976310/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140308462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of genetic polymorphisms on oral health-related quality of life after root canal treatment. 基因多态性对根管治疗后口腔健康相关生活质量的影响。
Brazilian dental journal Pub Date : 2024-03-22 eCollection Date: 2024-01-01 DOI: 10.1590/0103-6440202405678
Graciane E R Queiroz, Thuanny Castilho, Ludmila Silva Guimarães, Vania Gomes Moraes, Erlange Andrade Borges da Silva, Erika Calvano Küchler, Alice Corrêa Silva-Sousa, Manoel Damião Sousa-Neto, Lívia Azeredo Alves Antunes, Leonardo Santos Antunes
{"title":"Influence of genetic polymorphisms on oral health-related quality of life after root canal treatment.","authors":"Graciane E R Queiroz, Thuanny Castilho, Ludmila Silva Guimarães, Vania Gomes Moraes, Erlange Andrade Borges da Silva, Erika Calvano Küchler, Alice Corrêa Silva-Sousa, Manoel Damião Sousa-Neto, Lívia Azeredo Alves Antunes, Leonardo Santos Antunes","doi":"10.1590/0103-6440202405678","DOIUrl":"10.1590/0103-6440202405678","url":null,"abstract":"<p><p>To evaluate the impact of genetic polymorphisms in interleukins (IL1A rs17561, rs1304037; IL10 rs1800871; IL1RN rs9005), nitric oxide (NOS2 rs2779249, rs2897518) and suppressor of cytokine signaling (SOCS1 rs243327, rs33977706) on oral health-related quality of life (OHRQoL) of patients under-going root canal treatment (RCT).</p><p><strong>Methods: </strong>The sample consisted of 108 participants, presenting single-rooted teeth with asymptomatic periapical periodontitis. The impact of the OHRQoL was recorded using the Oral Health Impact Profile (OHIP-14) before, seven, and 30 days after RCT. Saliva samples were collected as a source of genomic DNA. Genetic polymorphisms were genotyped by Real-Time PCR using the Taqman method. Univariate and Multivariate analyses were used (p<0.05).</p><p><strong>Results: </strong>A significant difference was observed for the polymorphism rs2297518 in the NOS2 gene in functional limitation in the codominant (p=0.037) and recessive (p=0.001) models; in the physical pain (p<0.001 in both models); in psychological discomfort (p<0.001 in both models); in physical disability (p<0.001 in both models) and in psychological disability (p<0.001 in both models). Polymorphisms in the SOCS1 gene, in the recessive model, rs33977706 (p=0.045) and rs243327 (p=0.019), influenced the OHRQoL in the psychological discomfort domain.</p><p><strong>Conclusions: </strong>Polymorphisms in NOS2 and SOCS1 genes influenced the OHRQoL of patients undergoing RCT.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e245678"},"PeriodicalIF":0.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10976299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140308466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Natural canal deviation and dentin thickness of mesial root canals of mandibular first molars assessed by microcomputed tomography. 通过微型计算机断层扫描评估下颌第一磨牙中侧根管的自然偏差和牙本质厚度。
Brazilian dental journal Pub Date : 2024-03-22 eCollection Date: 2024-01-01 DOI: 10.1590/0103-6440202405648
Carolina Alonso Amorim, Marília F Marceliano-Alves, Isabelle Louise Gomes, José C Provenzano, Flávio R F Alves
{"title":"Natural canal deviation and dentin thickness of mesial root canals of mandibular first molars assessed by microcomputed tomography.","authors":"Carolina Alonso Amorim, Marília F Marceliano-Alves, Isabelle Louise Gomes, José C Provenzano, Flávio R F Alves","doi":"10.1590/0103-6440202405648","DOIUrl":"10.1590/0103-6440202405648","url":null,"abstract":"<p><p>The aim of this study was to assess the centralization and dentin thickness of mesial root canals of the first mandibular molars by microcomputed tomography (micro-CT). Material and methods: Ninety-nine mandibular molars of Vertucci's type IV canals were scanned by micro-CT. The mesiodistal deviation and centroid were assessed, in both mesiobuccal (MB) and mesiolingual (ML) canals, for the apical 4mm and the full canal length. Results: The dentin thickness was similar for both MB and ML canals. The narrowest thickness was in the distal wall of an MB canal (0.07mm), while the widest was found in the mesial wall of an MB canal (2.46mm). In centroid analysis, both the MB and ML canals exhibited deviations when compared to the root centroid, along the full canal length and the apical 4mm. For the MB canal, the mean deviation was 0.83mm (0.02 mm-2.30 mm) for the full canal and 0.18mm (0.01 mm-1.01 mm) for apical 4mm. Similarly, for the ML canal, the mean deviation measured 0.83 mm (0.05mm-3.99mm) for the full canal and 0.21 mm (0.01mm-1.01mm) for the apical 4 mm. Overall, deviations were observed towards the mesial of the roots, with 69% for MB and 57% for ML canals for the full canal, and 51% for MB canals within the 4 mm. The exception was the ML canal, which exhibited a higher deviation towards distal in the apical 4mm, accounting for 52% of cases. The dentin thickness was consistent between the mesial canals of mandibular molars. However, there is no centrality of mesial canals in their roots, with frequent deviation to mesial.</p>","PeriodicalId":101363,"journal":{"name":"Brazilian dental journal","volume":"35 ","pages":"e245648"},"PeriodicalIF":0.0,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10976306/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140308467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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