{"title":"T-Proteine des Streptococcus pyogenes I. Mitteilung: Präparation eines serologisch typenspezifischen T1-Antigens durch Ionenaustauschchromatographie und dessen Charakterisierung","authors":"K.-H. Schmidt , O. Kühnemund, W. Köhler","doi":"10.1016/S0172-5599(80)80082-4","DOIUrl":"10.1016/S0172-5599(80)80082-4","url":null,"abstract":"<div><p>The T-protein of <em>Streptococcus pyogenes</em> type 1 was trypsin-extracted and subsequently purified by ion exchange chromatography on CM and DEAE cellulose. Fractionation on CM cellulose by stepwise increase of the pH did not result in separation of type specific material from cross reacting components. The bulk of serologically active material was eluted at pH 5.6. On DEAE cellulose a type specific fraction was eluted with 0.05 m phosphate buffer, pH 8.2. A second fraction eluted with 0.25 m NaCl in the same buffer contained type specific as well as cross reacting material. Molecular weight distributions of type specific T-protein were studied by gel chromatography on Ultrogel ACA 44 and Biogel A 1.5 m. A multiple size subunit structure of T-protein was found and supported by SDS electrophoresis. Molecular weights of fragments serologically active in double diffusion were detected in a range of 37000 to more than 200000 Dalton. The isoelectric point was determined as to be pH 4.5. The purified T-protein was found to be free of cystein and of the amino sugars N-acetyl-glucosamine and N-acetyl-muramic acid.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"246 4","pages":"Pages 475-488"},"PeriodicalIF":0.0,"publicationDate":"1980-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80082-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87167501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Josef Richard Möse , Marianne Moser, Gerald Fischer
{"title":"Reduzierung der Histaminwirkung durch onkolytisch und nicht onkolytisch wirksame Clostridienstämme","authors":"Josef Richard Möse , Marianne Moser, Gerald Fischer","doi":"10.1016/S0172-5599(80)80087-3","DOIUrl":"10.1016/S0172-5599(80)80087-3","url":null,"abstract":"<div><p>Spores and vegetative forms of oncolytically active and oncolytically non-active clostridial strains were tested for histamine reducing activity (isolated ileum of guinea pig used for testing, waterbath method acc. to <em>Magnus</em>, Fig. 1).</p><p>Culture media and the washed intact material did not show any or only very little effectiveness. On the other hand the fractionated, charges diminished histamine standard solutions except for very little remaining activity in the case of all strains. This effect was stronger in vegetative forms than in spores (Fig. 2).</p><p>The histamine effect on the isolated organ was generally increased by means of culture media as well as by the washed intact material. In this respect, preparations of non-lytic strains showed a slightly higher effectiveness than preparations of lytic clostridial strains (Fig. 2).</p><p>It was shown in earlier investigations (cf. bibliography) that the charges of clostridial strains decompose plasmakinin (synthetic bradykinin) at varying rates.</p><p>Reduction of histamine activity by means of clostridia could lead to a decrease of the microcirculation and capillary-permeability in the tumor tissue (as hypothesized for brady-kinin) and thereby favour its disintegration.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"246 4","pages":"Pages 541-549"},"PeriodicalIF":0.0,"publicationDate":"1980-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80087-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83403810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alexander Hirschl , Gerold Stanek , Manfred Rotter
{"title":"Wirkungssteigerung von Fosfomycin durch Zusatz von Glukose-6-Phosphat bei intraperitoneal infizierten Mäusen","authors":"Alexander Hirschl , Gerold Stanek , Manfred Rotter","doi":"10.1016/S0172-5599(80)80090-3","DOIUrl":"10.1016/S0172-5599(80)80090-3","url":null,"abstract":"<div><p>The increase of antimicrobial activity of Fosfomycin by glucose-6-phosphate (G-6-P) has often been demonstrated by in vitro studies. However, this effect was never sufficiently established in vivo. Our study was carried out in order to investigate the effects of the addition of G-6-P to Fosfomycin in white mice (strain G.P. of N.I.H.), which were intraperitoneally infected with a strain of S. typhimurium (10<sup>3</sup> c.f.u/0.5 ml i.p.). One and six hours after the infection, 0.1 ml consisting of increasing doses of G-6-P (0, 5, 25, 50, 250 and 500 μg/g) and decreasing doses of Fosfomycin (15.6, 7.8, 3.9, 1.95, 0.98 and 0.49 μg/g) were applied i.m., using 10 animals for each combination. Tables 1 and 2 show the results of this therapy with regard to the reduction of the ED<sub>50</sub> of Fosfomycin. It can be seen that the addition of 25 μg G-6-P/g body-weight significantly reduces the ED<sub>50</sub> within the first days (Tab. 1), while doses of 50 and more μg G-6-P/g significantly reduce the ED<sub>50</sub> during the whole investigation period (Tab. 2). These results justify the proposal to use a combination of Fosfomycin and G-6-P in clinical studies for the treatment of human infections.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"246 4","pages":"Pages 562-566"},"PeriodicalIF":0.0,"publicationDate":"1980-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80090-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90256880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Abstracts of Papers Presented at the 3. Workshop of the Virology Section of the Deutsche Gesellschaft für Hygiene und Mikrobiologie Berlin, September 30 – October 4, 1979","authors":"","doi":"10.1016/S0172-5599(80)80081-2","DOIUrl":"10.1016/S0172-5599(80)80081-2","url":null,"abstract":"","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"246 4","pages":"Pages 429-474"},"PeriodicalIF":0.0,"publicationDate":"1980-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80081-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91088333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Zur Systematik von Actinobacillus, Haemophilus und Pasteurella: Basenzusammensetzung der DNS, Atmungschinone und kulturellbiochemische Eigenschaften repräsentativer Sammlungsstämme","authors":"W. Mannheim , Sabine Pohl , Und R. HollÄnder","doi":"10.1016/S0172-5599(80)80086-1","DOIUrl":"10.1016/S0172-5599(80)80086-1","url":null,"abstract":"<div><p>In a comparative study, 63 collection cultures representing 38 nomenspecies of, or assigned to, the genera <em>Actinobacillus, Haemophilus</em>, or <em>Pasteurella</em> were characterized by phenotypical features and deoxyribonucleic acid base composition. The latter was calculated from the thermal denaturation point. Biochemical reactions were tested in differential media commonly used for <em>Enterobacteriaceae</em>, and two test procedures were compared: (i) pure cultures with haematin and nicotine adenine dinucleotide added, where necessary, and (ii) xenocultures with an asaccharolytic <em>Acinetobacter</em> strain (ST 661/60). Furthermore, the respiratory quinones, and the effect of fumarate on oxygen-limited growth were considered. On the basis of these and some additional physiological and morphological criteria, a definition of the <em>Actinobacillus-Haemophilus-Pasteurella</em> group as a whole was established which appears to rank as a family. Several misclassified species, i.e. the socalled <em>Actinobacillus actinoides, Haemophilus piscium, Haemophilus vaginalis, Pasteurella anatipestifer</em>, and the organisms of the Bovine Lymphangitis group were eliminated, and the position of so-called <em>Pasteurella piscicida</em> was questioned. Some principles of subdivision of the group, and some of the practical identification procedures were discussed.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"246 4","pages":"Pages 512-540"},"PeriodicalIF":0.0,"publicationDate":"1980-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80086-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77930889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Survival of Listeria monocytogenes in High Salt Concentrations","authors":"M. Shahamat, A. Seaman, M. Woodbine","doi":"10.1016/S0172-5599(80)80085-X","DOIUrl":"10.1016/S0172-5599(80)80085-X","url":null,"abstract":"<div><p>The bactericidal effects of high concentrations of common salt has been determined in a laboratory medium for <em>Listeria monocytogenes</em> strain (1, 2a, No. 18). The survival time for the organism was followed at three different incubation temperatures (4, 22, 37 °C). The influence of temperature on the action of salt is discussed.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"246 4","pages":"Pages 506-511"},"PeriodicalIF":0.0,"publicationDate":"1980-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80085-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79781432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"T-Proteine des Streptococcus pyogenes II. Mitteilung: Präparation spezifischer Immunadsorbentien zur Isolierung von T-Antikörpern und Untersuchungen der T4/24… Agglutinationsgruppe","authors":"K.-H. Schmidt , O. Kühnemund, W. Köhler","doi":"10.1016/S0172-5599(80)80083-6","DOIUrl":"10.1016/S0172-5599(80)80083-6","url":null,"abstract":"<div><p>The T4-antigen(s) in <em>Streptococcus pyogenes</em> standard type strains of the T4/24… pattern was (were) investigated in regard to the uniformity or diversity of the antigen(s) in this T-complex. Tryptic T-extracts of types 4, 24, 26, 28, 29, 46, 48, 60 were purified by ion exchange chromatography on DEAE-cellulose. Anti-T4-antibodies were isolated by immunoadsorption chromatography on AH-sepharose linked T4-antigen.</p><p>Purified T4-antigen showed in SDS-electrophoresis a similar multiple molecular size structure as T1-antigen described earlier. Comparative serological studies of T-antigens of types 4, 24, 29 and 46 revealed reactions of identity to anti-T 4-antibodies in <em>Ouchterlony</em> tests. Extracts of types 26, 28, 48 and 60 did not precipitate with anti-T 4-antibodies, but types 28 and 48 showed crossreaction to the relevant antisera (anti-T28 and anti-T48, resp.) obviously caused by traces of R-28 antigen in both antigen preparations. Strains of the types 4, 24, 29, 46, 48 and 60 were agglutinated by anti-T 4-antibodies. The reaction could be inhibited by T4-antigen. The strains of types 26 and 28 used in our experiments did not contain T4-antigen.</p><p>Agglutination as well as immunoprecipitation reactions with specific antibodies prepared by immunochromatography proved the existence of common T4-antigenic determinants in types 4, 24, 29, 46, 48 and 60.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"246 4","pages":"Pages 489-498"},"PeriodicalIF":0.0,"publicationDate":"1980-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80083-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79005514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Inhaltsverzeichnis","authors":"","doi":"10.1016/S0172-5599(80)80091-5","DOIUrl":"https://doi.org/10.1016/S0172-5599(80)80091-5","url":null,"abstract":"","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"246 4","pages":"Pages 567-575"},"PeriodicalIF":0.0,"publicationDate":"1980-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80091-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137058640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Primary Immune Response of Mice to Sheep Erythrocytes During the Course of Infection with Listeria monocytogenes","authors":"C.H. Wirsing , v. König , H. Finger","doi":"10.1016/S0172-5599(80)80114-3","DOIUrl":"10.1016/S0172-5599(80)80114-3","url":null,"abstract":"<div><p>The influence of an infection with a sublethal dose of viable <em>Listeria monocytogenes</em> on the primary antibody-forming potential of mice against sheep erythrocytes (SE) was monitored by means of counting plaque-forming cells (PFC) in the spleen and measuring circulating antibodies against SE. Additionally, the course of infection was followed by determination of viable bacteria in the spleen.</p><p>As against the injection of killed <em>L. monocytogenes</em>, infection with viable bacteria did not display any significant influence on the immune response against SE when the immunogenic stimulus was given either simultaneously with the bacteria or on day 4 or 13 of infection, respectively. Vice versa, the immunization with the particulate antigen had no measurable influence on either course or outcome of infection with the parasite.</p></div><div><p>Der Einfluß einer Infektion mit einer subletalen Dosis lebender <em>Listeria monocytogenes</em> Organismen auf das primäre Antikörperbildungsvermögen von Mäusen gegen Schaferythrocyten wurde mittels Anwendung der direkten und indirekten Plaquetechnik und durch Bestimmung der zirkulierenden Antikörper gegen Schaferythrocyten untersucht. Zusätzlich wurde der Verlauf der Infektion durch die Zählung der lebenden Listerien in den Milzen der Tiere überwacht. Im Gegensatz zu der Applikation abgetöteter <em>L. monocytogenes</em> Organismen, über deren adjuvanten Einfluß auf die primäre Immunantwort wiederholt berichtet worden ist, zeigte die Infektion mit lebenden Bakterien keinerlei Auswirkung auf die Entwicklung Plaque-bildender Zellen (Abb. 1). Analog dazu wurde auch die Infektion durch die gleichzeitige Immunisierung nicht meßbar beeinflußt (Abb. 2). Gleiches gilt, wenn die Injektion der Schaferythrocyten am 4. bzw. 13. Tag nach der Infektion erfolgte. Auch hier war weder eine Änderung der Immunantwort (Abb. 3) noch eine Beeinflussung des Infektionsverlaufs oder der Infektionsüberwindung festzustellen (Abb. 4). Es kann daher gefolgert werden, daß eine Infektion mit <em>L. monocytogenes</em> nicht zu einer Steigerung der Immunantwort gegenüber einem nicht verwandten Antigen führt.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"246 2","pages":"Pages 204-210"},"PeriodicalIF":0.0,"publicationDate":"1980-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80114-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74804106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}