Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie最新文献

{"title":"Ein Beitrag zur Durchführung der Fluoreszenzserologie bei Leptospirose","authors":"C. Staak ,&nbsp;A. SchÖnberg","doi":"10.1016/S0172-5599(80)80030-7","DOIUrl":"10.1016/S0172-5599(80)80030-7","url":null,"abstract":"<div><p>Mono- and polyvalent FITC-conjugates were prepared for detection of leptospires in artificially infected material. The organisms were concentrated by centrifugation and fixed onto glass slides after they had been mixed with serum or egg albumin. By this method, leptospires were detected down to a concentration of 10²-10⁴ organisms/ml</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"247 1","pages":"Pages 138-141"},"PeriodicalIF":0.0,"publicationDate":"1980-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80030-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81535290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aerosolimpfung von Mäusen mit inaktiviertem Influenzavirus","authors":"Manfred Neukirch ,&nbsp;Kurt Bauer","doi":"10.1016/S0172-5599(80)80016-2","DOIUrl":"10.1016/S0172-5599(80)80016-2","url":null,"abstract":"<div><p>NMRI mice were vaccinated by the aerosol technique, using the ethylethylenimine inactivated and polyethylenglycol concentrated virus strain A/PR/8/34 (HO/N1) with or without addition of Bordetella pertussis extract (BPE) as an adjuvant. The immune response of the vaccinated animals was controlled by challenge infection via aerosol technique and by examination of HAI antibodies in the serum and in the washings of lungs.</p><p>After a single aerosol vaccination a weak protection was observed only, if the vaccines contained BPE. But a second immunization with a vaccine containing BPE induced a high degree of immunity, even if a reduced amount of antigen was used for booster vaccination. After three aerosol vaccine doses in two-weeks intervals, however, we were unable to infect the immunized animals even with largest amounts of challenge virus (approximately 50000 LD₅₀).</p><p>The addition of BPE as an adjuvant induced a significantly better protection and resulted in much higher titres of HAI antibodies in the serum and in the respiratory tract compared to mice vaccinated by the same procedure but without BPE.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"247 1","pages":"Pages 18-24"},"PeriodicalIF":0.0,"publicationDate":"1980-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80016-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90659388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation, Purification, and Properties of Neuraminidase from Propionibacterium acnes","authors":"Hubertus Von Nicolai ,&nbsp;Ulrich Höffler ,&nbsp;Fritz Zilliken","doi":"10.1016/S0172-5599(80)80024-1","DOIUrl":"10.1016/S0172-5599(80)80024-1","url":null,"abstract":"<div><p>Neuraminidase activity was discovered in 32 of 38 strains of <em>Propionibacterium acnes</em>. Enzyme production was studied in yeast extract bouillon of different pH containing various amounts of human milk as neuraminidase inductor.</p><p>Enzyme activity was found in the bacterial sediments as well as in the culture filtrates. Since neither ultrasonic treatment nor lysozyme incubation of bacterial sediments did release reasonable amounts of enzyme, culture filtrates were used for enzyme preparation.</p><p>Neuraminidase was isolated by 40% ammonium sulfate precipitation, dialysis, concentration and repeated gel chromatography on Sephadex G-100. The enzyme possesses a molecular weight of about 33000 and a pH-optimum around 5.0. The Michaelis constants are 1.8 × 10^-3 M for α2 → 3 linked N-acetylneuraminic acid (NeuAc) in II³NeuAc-Lac, 3.7 × 10^-3 M for the α2 → 6 linkage in II⁶NeuAc-Lac, and 2.1 × 10^-3 M for the α2 → 8 linkage of II³ (← 2αNeuAc8)₂-Lac, respectively.</p><p>Among the different groups of naturally occurring NeuAc-containing substrates, i.e. oligosaccharides, glycolipids and glycoproteins, the enzyme exhibits its highest activity towards low molecular weight oligosaccharides. Activity is considerably lower on glycoproteins. Glycolipids (gangliosides) are only little attacked under conditions used in the test. However, there is no remarkable specificity towards one of the different linkage types of N-acetylneuraminic acid. In general, the enzyme reveals a specificity pattern similar to that found in other bacteria of low pathogenicity towards man.</p></div><div><p>Neuraminidase konnte in 32 von 38 verschiedenen Stämmen von <em>Propionibacterium acnes</em> nachgewiesen werden. Die Syntheseraten wurden in Yeast-Extrakt-Bouillon in Abhängigkeit vom pH-Wert und von als Neuraminidase-Induktor hinzugesetzter Frauenmilch bestimmt.</p><p>Enzymaktivitäten fanden sich sowohl in den Bakteriensedimenten, als auch in den Kulturfiltraten. Das Enzym aus dem Bakteriensediment konnte weder durch Ultraschall noch durch Lysozymbehandlung in nennenswerten Mengen freigesetzt werden. Dies gelang jedoch teilweise durch wiederholtes Einfrieren und Auftauen. Die Reinigung erfolgte mittels Ammoniumsulfat-Fällung (40% Sättigung), Dialyse, Konzentrierung und mehrmaliger Gel-Chromatographie an Sephadex. Das Enzym besitzt ein Molekulargewicht von ungefähr 33000 und ein pH-Optimum um 5,0. Die Michaelis-Konstanten (K_m) betragen 1.8 × 10^-3 M für α2 → 3-gebundene N-Acetylneuraminsäure (NeuAc) in II³NeuAc-Lac, 3,7 × 10^-3 M für α2 → 6-gebundene in II⁶NeuAc-Lac und 2,1 × 10^-3 M für die α2 → 8-Bindung in II³ (← 2αNeuAc8)₂-Lac.</p><p>Unter den verschiedenen Gruppen natürlich vorkommender NeuAc-haltiger Substrate, speziell Oligosaccharide, Glycolipide und Glycoproteine, werden die niedermolekularen Oligosa","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"247 1","pages":"Pages 84-94"},"PeriodicalIF":0.0,"publicationDate":"1980-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80024-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17506910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nachweis von Leptospiren-Antikörpern in Seren immunisierter Kaninchen mit Hilfe des indirekten Immunfluoreszenztestes","authors":"W. FrÖhlich","doi":"10.1016/S0172-5599(80)80028-9","DOIUrl":"10.1016/S0172-5599(80)80028-9","url":null,"abstract":"<div><p>Antibodies of <em>Leptospirae</em> were demonstrated in the sera of immunized rabbits by the indirect immunofluorescence test, only after a suitable dilution of the “anti-rabbit-gammaglobulin fluorescin conjugated” was carried out in a cross reference titration (“chessboard method”). A distinct crossreaction was observed when anti-<em>icterohaemorrhagiae</em> — and anti-<em>bratislava</em>-immunsera reacted with several serotypes out of the “<em>interrogans complex</em>”. But only a weak crossreaction was found when both sera reacted with the serotyp patoc from the “<em>biflexa complex</em>”. The evaluation of the indirect immunofluorescence test was complicated by a streaky background of fluorescence. The agargeldiffusion tests (<em>Ouchterlony</em>) exhibited four, three and two precipitation bands according to the combination of antigens and immunsera.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"247 1","pages":"Pages 124-131"},"PeriodicalIF":0.0,"publicationDate":"1980-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80028-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78501782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zur Phagenkonversion von Clostridium novyi Typ A","authors":"Gisela Schallehn ,&nbsp;M.W. Eklund ,&nbsp;H. Brandis","doi":"10.1016/S0172-5599(80)80025-3","DOIUrl":"10.1016/S0172-5599(80)80025-3","url":null,"abstract":"<div><p><em>Clostridium novyi</em> type A, the cause of gas gangrene in man and animal, produces four main toxic components (alpha, gamma, delta, and epsilon). The alpha toxin is a necrotizing toxin produced in lethal amounts by strains of type A. This lethal toxin induces a characteristic subcutaneous colorless gelatineous edema when guinea pigs are infected with <em>C. novyi</em> type A.</p><p>In this paper we report further evidence on the relationship of bacteriophages to the production of alpha toxin by <em>C. novyi</em> type A. The results showed that five phage-sensitive bacterial strains that did not produce alpha toxin could be isolated from alpha toxin producing strains. These nontoxigenic strains could be infected with specific bacteriophages isolated from the different toxigenic strains of <em>C. novyi</em> type A and converted to alpha toxin production. The toxigenicity of these converted strains depended upon the continued participation of specific bacteriophages. These bacteriophages were shown to be very unstable in culture supernatant fluids.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"247 1","pages":"Pages 95-100"},"PeriodicalIF":0.0,"publicationDate":"1980-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80025-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82798393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infectious Drug Resistance in E. coli Isolated from Livestock","authors":"A.I. Adetosoye","doi":"10.1016/S0172-5599(80)80017-4","DOIUrl":"10.1016/S0172-5599(80)80017-4","url":null,"abstract":"<div><p><em>Escherichia coli</em> strains isolated from clinically healthy and sick animals in Lagos and Oyo States of Nigeria were tested for drug resistance and distribution of R-factors. Fifteen multiple and 3 single (OT, S, S₃) antibiotic resistance patterns were obtained among 333 isolates. Of the 285 resistant strains, 133 transfered their resistant determinant to sensitive <em>E. coli</em> K12 recipients in part or as a block. None of the donors had sex pili and they were not lysed by Ms2 phage. It required about 2 h for <em>E. coli</em> K12 to acquire a resistance determinant from <em>E. coli</em> strains Nos. 295, 62 and 67 habouring resistance to oxytetracycline, streptomycin and compound of sulphonamides (S₃). The number of recipient cells that formed transconjugants increased with the time of incubation.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"247 1","pages":"Pages 25-34"},"PeriodicalIF":0.0,"publicationDate":"1980-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80017-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17174493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemische und biologische Eigenschaften von Revertanten aus einer Salmonella typhimurium-Rd1-Mutante","authors":"Siegfried Schlecht ,&nbsp;Inge Fromme ,&nbsp;Ernst Ferber ,&nbsp;Werner Müller ,&nbsp;Jobst Gmeiner","doi":"10.1016/S0172-5599(80)80020-4","DOIUrl":"10.1016/S0172-5599(80)80020-4","url":null,"abstract":"<div><p>Two S-form-revertant strains were isolated from a <em>S. typhimurium</em> Rd₁ culture on account of their phage resistance. In microbiological and serological (O-agglutination) characterization — as well as in stability tests (agglutination in auramin and saline and heating at 100 °C) — the behaviour of the two strains was the same as that of the wild type. The two strains were found to be indistinguishable from the wild type strain also with respect to the chemical composition of their lipopolysaccharides. Thus the amount and proportion of fatty acids and sugar residues as well as the number of repeating units in the O-chain were all identical. In contrast, the isolated revertants were similar to the Rd₁ mutant with respect to their auxotrophic markers methionine and tryptophane, to the absence of flagella as well as to the reduced content of cyclopropane fatty acids (C₁₇, C₁₉). Protein analysis revealed no significant qualitative or quantitative differences between the wild type strain and the two revertants with respect to the major proteins of their outer membranes.</p><p>The sensitivity of the revertants to crystal violet, erythromycin and rifamycin SV was intermediate between the wild type and the Rd₁ mutant. Their temperature maximum in nutrient broth was 43 °C, the retardation in growth at this temperature corresponding to that of the Rd₁ mutant. At 37 °C, however, the growth rate of the revertants was identical to that of the wild-type, while that of the Rd₁ mutant was slower. Addition of sodium chloride to the growth medium rendered the temperature dependent behaviour of the mutants and revertants similar to that of the wild type. Studies in NMRI mice revealed that the revertants, also with regard to their virulence, occupy an intermediate position between the mutant and the wild type. Nevertheless their ability to afford protection to <em>Salmonella typhimurium</em> infection following active immunization with acetone killed cells was as high as that of the wild type.</p><p>The results show that the biologic behaviour of <em>S. typhimurium</em> is determined by the type of lipopolysaccharide it contains but also to a large extent by other cell-wall constituents.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"247 1","pages":"Pages 50-63"},"PeriodicalIF":0.0,"publicationDate":"1980-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80020-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83476779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histoautoradiographic Studies of 3H-Thymidine Labelled Erysipelothrix rhusiopathiae Introduced into Albino Mice","authors":"T. Todorov ,&nbsp;As. Toshkov ,&nbsp;M. Anastassova-Kristeva ,&nbsp;Y. Martinova ,&nbsp;L. Kancheva","doi":"10.1016/S0172-5599(80)80084-8","DOIUrl":"10.1016/S0172-5599(80)80084-8","url":null,"abstract":"<div><p>S-forms of <em>E. rhusiopathiae</em> were cultivated in a ³H-thimidine-containing medium. A suspension washed three times in succession was intravenously injected into albino mice. At certain intervals liver, spleen and kidney were examinated by routine autoradiography. Intact labelled bacteria were found as early as 5 min after administration in the blood capillaries, around cells of the RES and in some nuclei of hepatocytes. Later they are detected in the cytoplasm of micro- and macrophages, around megacaryocytes and in some of their nuclei. After 6 h the labelled population sharply decreases due to a very active reproduction and mortality of the initial population.</p><p>The localisation of bacteria in liver nuclei and the spreading of S-forms of <em>E. rhusiopathiae</em> in comparison with the avirulent L-forms therefrom are discussed.</p></div><div><p>S-Formen von <em>E. rhusiopathiae</em> wurden in einem ³H-Thymidin enthaltenden Medium kultiviert. Eine Bakteriensuspension wurde nach dreimaligem Waschen intravenös in Albinomäuse injiziert. Nach verschiedenen Intervallen wurden Leber, Milz und Nieren autoradiografiert. Intakte markierte Bakterien wurden schon 5 Min. nach der Injektion in den Blutkapillaren, im Bereich des RES und in einigen Kernen von Hepatozyten gefunden. Später konnten sie in dem Zytoplasma der Mikro- und Makrophagen, im Bereich von Megakariozyten und in einigen ihrer Kerne nachgewiesen werden. Nach 6 Std. fiel die Zahl markierter Zellen scharf ab als Folge einer sehr aktiven Reproduktion und des Absterbens der Zellpopulation. Die Lokalisierung von Bakterien in den Leberzellkernen und die Verbreitung von S-Formen der <em>E. rhusiopathiae</em> im Vergleich mit deren avirulenten L-Formen wurden diskutiert.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"246 4","pages":"Pages 499-505"},"PeriodicalIF":0.0,"publicationDate":"1980-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80084-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75275793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nachweis von Chlamydia trachomatis in der Gewebekultur","authors":"Veronique Mösinger-Lundgren ,&nbsp;Detlef Petzoldt","doi":"10.1016/S0172-5599(80)80089-7","DOIUrl":"10.1016/S0172-5599(80)80089-7","url":null,"abstract":"<div><p>The method for the identification of <em>Chlamydia trachomatis</em> in tissue culture described by <em>Hobson</em> was slightly modified and tested as to its usefullness for routine-diagnostic. Cervical swabs of 74 prostitutes and 100 unselected gynecological outpatients were examined for <em>Chlamydia trachomatis</em>. The isolation of Chlamydia was carried out by <em>McCoy</em> cell culture; the inclusions were identified by <em>Giemsa</em> and <em>Lugol</em>'s stain procedure.</p><p>The isolation rate of <em>Chlamydia trachomatis</em> was 17% in the group of the prostitutes, whereas in the group of unselected women no Chlamydia were detected.</p><p>The correspondence of our findings with previous results indicates the applicability of the method used.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"246 4","pages":"Pages 555-561"},"PeriodicalIF":0.0,"publicationDate":"1980-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80089-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84357642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparative Study on the Pretreatment Methods for the Cultivation of Mycobacterium tuberculosis on LJ Medium Part II","authors":"Muhammad Aslam Khan ,&nbsp;Peerzada M.A. Siddiqui","doi":"10.1016/S0172-5599(80)80088-5","DOIUrl":"10.1016/S0172-5599(80)80088-5","url":null,"abstract":"<div><p>The sputa of 231 cases, who were admitted to Ojha Institute of Chest Diseases, Karachi were studied in order to compare the efficiency and reliability of three pretreatment methods viz. Standard method, <span><em>Marks'</em> method (1959)</span>, and <span><em>Becker's</em> method (1961)</span>. Out of the 231 specimens, 132 were film positive while 99 were film negative. 141 cases were found positive after cultivation on LJ medium. The number of cases found to be positive with Standard method, <em>Marks'</em> method and <em>Becker's</em> method were 91, 84, 105 respectively. Contaminations encountered with Standard method were 43, with <em>Marks'</em> method 41, and with <em>Becker's</em> method 20. 97 negatives were observed with Standard method and 106 each with <em>Marks'</em> and <em>Beckers'</em> methods. The merits and demerits of the three methods are discussed.</p></div><div><p>231 Sputumproben aus dem Ojha-Institut für Brustkrankheiten in Karachi wurden untersucht, um die Wirksamkeit und Verläßlichkeit dreier Vorbehandlungsmethoden, nämlich der Standardmethode, der <span><em>Marek's</em> Methode (1959)</span> und der Methode von <span><em>Becker</em> (1961)</span>, zu vergleichen. Bei den 231 Proben erwiesen sich 132 als film-positiv und 99 als film-negativ. 141 Proben zeigten sich nach Züchtung auf LJ-Medium als positiv. Die Anzahl der positiven Proben war folgende: Standardmethode: 91, <em>Marek's</em> Methode: 84 und die Methode von <em>Becker:</em> 105. Die Verunreinigungen bei der Standardmethode betrugen 43, 41 bei der <em>Marek's</em> Methode und 20 bei der Methode von <em>Becker</em>. 92 Proben erwiesen sich bei der Standardmethode als negativ, bei den beiden anderen Methoden betrug die Anzahl jeweils 106. Vor- und Nachteile der drei Methoden werden diskutiert.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"246 4","pages":"Pages 550-554"},"PeriodicalIF":0.0,"publicationDate":"1980-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80088-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90121755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}