Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie最新文献

{"title":"Antistreptococcal Antibodies, Rheumatoid Factors and Haptoglobin in Apes and Monkeys","authors":"W. Köhler ,&nbsp;Eteri K. Djikidse ,&nbsp;W.W. Socha ,&nbsp;O. Prokop ,&nbsp;D. Gerlach ,&nbsp;Rosemarie Otto ,&nbsp;Elisabeth Balaeva","doi":"10.1016/S0172-5599(80)80068-X","DOIUrl":"10.1016/S0172-5599(80)80068-X","url":null,"abstract":"<div><p>Sera of 157 baboons <em>(Papio hamadryas</em> and <em>P. anubis)</em> 21 chimpanzees <em>(Pan troglodytes)</em> five orang utans <em>(Pongo pygmaeus)</em> three mountain gorillas <em>(Gorilla gorilla beringei)</em> and three gibbons <em>(Hylobates lar lar)</em> were examined for the content of antistreptolysin O, antidesoxyribonuclease B, for the presence of rheumatoid factors as well as for the level and type of haptoglobin.</p><p>The mean antistreptolysin O titer (AST) in baboons was 106 ASU ± 18 in dextransulfate absorbed sera (“real” AST) and 182 ASU ± 34 in non-absorbed sera. The mean decrease after absorption was 20% (i.e., 20% inhibitor + 80% antistreptolysin O), a value that is lower than previously found in rhesus monkeys (55%) or in man (40%). Raised values of anti-DNase B were found in two baboons only, and none of the sera of that species displayed presence of rheumatoid factors.</p><p>In chimpanzees, the mean AST was 68 ± 29 in absorbed and 87 ASU ± 57 in non-absorbed sera. Anti-DNase B was raised in three animals, and in two cases the increase was correlated with raised AST. Of 19 chimpanzee sera examined, 13 were found to contain antigammaglobulins (“rheumatoid factors”) the titers of which reached 1:64 or more.</p><p>All primate sera tested so far showed haptoglobin type 1-1 or Hp 1-1-like patterns. The haptoglobin level in chimpanzees and baboons was comparable to that established in man; in rhesus monkey, on the other hand, much lower values (40–62 mg/100ml) or ahaptoglobinemia were observed. The sera of all monkeys and apes tested so far showed a very low (undiluted, or up to 1:10 titer at most) agglutinating activity against T4-antigencarrying streptococci. This is in agreement with our observations made previously which indicated that human or animal sera of haptoglobin type 1-1 agglutinated streptococci to a much lower degree than type Hp 2-2 or type Hp 2-1 sera.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"246 3","pages":"Pages 308-317"},"PeriodicalIF":0.0,"publicationDate":"1980-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80068-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75673468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Untersuchungen über den Verlust von R-Plasmiden bei E. coli und Klebsiella pneumoniae im Verlauf von Bouillonpassagen","authors":"N. Dickgiesser","doi":"10.1016/S0172-5599(80)80069-1","DOIUrl":"10.1016/S0172-5599(80)80069-1","url":null,"abstract":"<div><p>Plasmid RP1 (= Carb^rNeo^rKana^rTetra^rAmp^r) or Plasmid R100 (= Tetra^rStrept^rSulf^r) were transferred on 5 different strains of <em>E. coli</em> and on 3 strains of <em>Kl. pneumoniae</em>. Plasmid RK1 ( = Carb^rTet^rStrept^rAmp^r) was transferred on 3 strains of <em>Kl. pneumoniae</em> also. The resulting strains were cultured in 7 passages of Standard-I-Nutrient-Broth (Merck) and Mueller-Hinton-Broth (Becton-Dickinson); 48 colonies were examined for resistance to antibiotics of the different R-Plasmids.</p><p>In <em>E. coli</em> and in <em>Kl. pneumoniae</em> with R-Plasmids RP1 and RK1 no more than 2 colonies without one of several antibiotic resistances after 7 nutrient broth passages were seen. Striking loss of R-Plasmids was seen in <em>Kl. pneumoniae</em> with R-Plasmid R100 after the third passage. The behaviour of strains in Standard-I-Nutrient-Broth and Mueller-Hinton-Broth was not different.</p><p>In conclusion for sensitivity test colonies should be taken only from primary cultures. Furthermore it is recommended to pick up several colonies from one isolated strain for sensitivity test.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"246 3","pages":"Pages 318-328"},"PeriodicalIF":0.0,"publicationDate":"1980-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80069-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83025629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serological Examinations with TPHA Antigen Prepared from T. pallidum Budapest Strain","authors":"István Horváth ,&nbsp;Márta Marschalkó,&nbsp;István Rácz","doi":"10.1016/S0172-5599(80)80080-0","DOIUrl":"https://doi.org/10.1016/S0172-5599(80)80080-0","url":null,"abstract":"<div><p>The TPHA test is performed with sheep erythrocytes treated with formalin and tannin solution and conjugated with fragments of pathogenic <em>Treponema pallidum</em>. Absorption media was prepared by adding normal rabbit serum, powdered rabbit testicle, sheep and cow stroma and sonicated Reiter treponeme. The reagents prepared in our laboratory proved to be specific and sensitive enough for routine use. It can be confirmed that the TPHA reaction is a good confirmatory test in the serodiagnosis of syphilis. Further studies are necessary to explain the divergent results between TPHA and TPIT.</p><p>We plan to carry out detailed investigation. Analysis of patients data in a large population after the problem of storage of our reagents over extended periods has been resolved.</p></div><div><p>Der TPHA-Test wird mit Schaferythrozyten, die formalin- und tanninbehandelt mit Fragmenten von pathogenen Treponema pallidum konjugiert sind, durchgeführt. Die Absorptionsmedien wurden nach Hinzufügen von getrocknetem normalen Kaninchenserum und Kaninchenhoden, Schaf- und Rinderstroma und mit durch Ultraschall behandelten Reitertreponemen versetzt. Die in unserem Laboratorium hergestellten Reagenzien erwiesen sich als spezifisch und empfindlich genug für den Routinegebrauch. Es kann bestätigt werden, daß die TPHA-Reaktion einen verläßlichen Test für die Serodiagnostik der Syphilis darstellt. Weitere Untersuchungen sind notwendig, um abweichende Resultate zwischen TPHA und TPIT zu erklären. Es wurde geplant, detaillierte Analyseuntersuchungen von Patienten im großen Umfang durchzuführen, nachdem das Problem der langzeitigen Lagerung der Reagenzien geklärt worden ist.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"246 3","pages":"Pages 423-427"},"PeriodicalIF":0.0,"publicationDate":"1980-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80080-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91739405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virusspezifischer IgM-Nachweis mit Routinemethoden","authors":"H.W. Doerr ,&nbsp;R. Fretschner,&nbsp;H.P. Geisen","doi":"10.1016/S0172-5599(80)80109-X","DOIUrl":"10.1016/S0172-5599(80)80109-X","url":null,"abstract":"<div><p>By the use of a specific immunosorption to insoluble adsorbentia (controlled-pore glass, polystyrene particles) the separation of IgM and IgG is performed in serum specimens, which originate from patients presenting several virus infections (mumps, measles, cytomegalovirus, herpes simplex), for the virus-specific IgM proof with routine serologic methods (CFT, HIT, NT). The results are in a good agreement to them seen in the demonstration of significant titer rises to assure the diagnosis of acute mumps and measles infections by HIT rapidly. While the new technique is also successfully applied for the determination of neutralizing IgM and IgA antibodies to HSV, no sufficient results are available to detect CMV specific IgM antibodies by CFT compared to other methods (IFT, ELISA).</p><p>Zusammenfassung</p><p>Über eine spezifische Immunadsorption an unlösliche Träger (controlled-pore glass, Polystyrolpartikel) wird die IgM/IgG-Trennung bei Serumproben von Patienten mit verschiedenen Virusinfektionen (Mumps, Masern, Cytomegalie, Herpes simplex) durchgeführt und für den virusspezifischen IgM-Antikörper-Nachweis mit virusserologischen Routine-methoden (KBR, HHT, NT) erprobt. Die Resultate belegen im Vergleich zur konventionellen Serodiagnostik (Bestimmung der Antikörperkinetik mit zwei oder mehr Serumproben zu einem Krankheitsfall) die Möglichkeit der Schnelldiagnostik mit dem Mumps- und Masern-HHT. Während die spezifische IgM- und IgA-Antikörperdiagnostik so auch beim NT (Beispiel Herpes simplex) anwendbar ist, fallen die Ergebnisse mit der Cytomegalie-KBR gegenüber den Referenzmethoden (IFT, ELISA) unbefriedigend aus.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"246 2","pages":"Pages 158-166"},"PeriodicalIF":0.0,"publicationDate":"1980-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80109-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72576756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On the Ecology of Yersinia enterocolitica O 3. Yersinia in Synanthropic Animals","authors":"Eva Aldová ,&nbsp;B. Skorkovský,&nbsp;J. Kapinus,&nbsp;Miluše Pejhovská,&nbsp;GruŽa SoukupovÁ","doi":"10.1016/S0172-5599(80)80072-1","DOIUrl":"10.1016/S0172-5599(80)80072-1","url":null,"abstract":"<div><p>Rats <em>(Rattus rattus)</em> and sewer rats <em>(R. norvegiens)</em> trapped outside in piggeries, small mouse-like rodents trapped in human habitations and swine from piggeries and slaughterhouses were examined. In rats, sewer rats and mouse-like rodents other serotypes of <em>Y. enterocolitica</em> than O3 were isolated. However, two out of five sewer rats caught at a slaughterhouse yielded <em>Y. pseudotuberculosis</em> I; this finding corresponded with some findings made in swine.</p><p>Swine positivity rates in piggeries corresponded to the hygienic conditions found there. In a hygienically backward farm six out of 14 piglets were positive, whereas in a piggery with a high standard of hygiene only one out of 44 piglets yielded <em>Y. pseudotuberculosis</em>. Specimens of colon contents from 318 slaughtered pigs yielded <em>Y. enterocolitica</em> O3 three times (0.94%), <em>Y. pseudotuberculosis</em> four times (1.26%) and <em>Y. enterocolitica</em> serotypes other than O3 18 times; a total of 19 swine (5.9%) were positive, some for two serotypes simultaneously, one for a combination of <em>Y. enterocolitica</em> O3 and <em>Y. pseudotuberculosis</em> I. Apart from this, <em>Plesiomonas shigelloides</em> was isolated from several slaughtered pigs and from one slaughterhouse rat; salmonellae were isolated from some slaughtered pigs, and a common finding was H₂S-positive <em>E. coli</em>.</p></div><div><p>Es wurden außerhalb von Schweinehaltungen gefangene Ratten der Arten Rattus rattus und R. norvégiens, kleine in menschlichen Wohnstätten gefangene mäuseartige Nager und Schweine aus Zuchtbetrieben sowie Schlachthäusern untersucht. Bei den Ratten und mäuseartigen Nagern wurden andere Serotypen von Y. enterocolitica als O 3 isoliert, 2 von 5 in Schlachthäusern gefangene Ratten (R. novegicus) wiesen Y. pseudotuberculosis I auf; dieser Befund stimmte mit einigen Befunden bei Schweinen überein.</p><p>Die Häufigkeit positiver Befunde in Schweinehaltungen zeigte eine Korrelation zu den hygienischen Zuständen. In einem hygienisch rückständigen Betrieb waren 6 von 14 Fer- kein positiv, wogegen in einem Betrieb mit hohem Hygienestandard nur eines von 44 Ferkeln <em>Y. pseudotuberculosis</em> aufwies. Aus dem Coloninhalt von 318 Schlachtschweinen wurde <em>Y. enterocolitica</em> O 3 dreimal (0,94%), <em>Y. pseudotuberculosis</em> viermal (1,26%) und <em>Y. enterocolitica</em> (andere Serotypen) 18 mal isoliert. Insgesamt waren 19 Schweine (5,9%) positiv, einige davon für 2 Serotypen gleichzeitig und eines für eine Kombination von <em>Y. enterocolitica</em> O 3 und <em>Y. pseudotuberculosis</em> I. Daneben wurde von verschiedenen Schlachtschweinen und einer Schlachthausratte <em>Plesiomonas shigelloides</em> isoliert, Salmonellen von einigen Schlachtschweinen sowie allgemein H₂S-positive <em>E. coli</em>.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"246 3","pages":"Pages 344-352"},"PeriodicalIF":0.0,"publicationDate":"1980-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80072-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75441346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolierung von Mykoplasmen aus dem Genitaltrakt von Stuten","authors":"Helga Kirchhoff ,&nbsp;Jan Heitmann ,&nbsp;Wolfgang Bisping","doi":"10.1016/S0172-5599(80)80116-7","DOIUrl":"10.1016/S0172-5599(80)80116-7","url":null,"abstract":"<div><p>Mycoplasmas were isolated from 11 (= 6,8%) of 161 cervix swabs from infertile mares. A total of 17 strains was isolated and characterized by indirect immunofluorescent test and metabolic inhibition test as <em>Mycoplasma equigenitalium</em> (11 strains), <em>Mycoplasma subdolum</em> (2 strains), <em>Acholeplasma laidlawii</em> (3 strains) and <em>Acholeplasma equifetale</em> (1 strain). In addition cervix swabs of the mares were investigated for bacteria. There was no specific correlation between presence of mycoplasmas and bacteria (table 1). In clinical investigations 5 of the 11 mares which harboured mycoplasmas showed a pneumovagina (table 1). The isolated mycoplasmas were tested for sensitivity to antibiotics (table 2). All of the isolates were sensitive to Chloramphenicol (10 μg), Tetracyclin (10 μg), Tylosin (30 μg) and Gentamycin (10 μg) and resistant to Penicillin (6 IU) and Polymyxin B (300 IU). Against Erythromycin (15 μg) all of the <em>Acholeplasma</em>-isolates were sensitive and all of the <em>Mycoplasma</em>-isolates resistant. Against Streptomycin (10 μg) the two <em>M. subdolum</em> strains showed resistance, whereas <em>M. equigenitalium</em> and the <em>Acholeplasma</em>-isolates were sensitive. It is not yet possible to elucidate the significance of acholeplasmas and mycoplasmas in the genital organs of mares.</p><p>Zusammenfassung</p><p>In der vorliegenden Untersuchung wurden aus 11 von 161 Zervixtupfern (= 6,8%) güster Stuten Mykoplasmen isoliert, die als <em>Mycoplasma equigenitalium</em> (11 Isolate), <em>Mycoplasma subdolum</em> (2 Isolate), <em>Acholeplasma laidlawii</em> (3 Isolate) und <em>Acholeplasma equifetale</em> (1 Isolat) bestimmt werden konnten. Zwischen den Mykoplasmennachweisen und den bakteriologischen Befunden ließen sich keine besonderen Beziehungen aufzeigen. In der klinischen Untersuchung ist die Häufung des Befundes mangelnder Schamschluß (5 der 11 mykoplasmenpositiven Stuten) auffällig. In der Prüfung auf Antibiotikaempfindlichkeit erwiesen sich alle Isolate als empfindlich gegenüber Chloramphenicol (10 μg), Tetracyclin (10 μg), Tylosin (30 μg) und Gentamycin (10 μg) und als resistent gegenüber Penicillin (6 I. E.) und Polymyxin B (300 I. E.). Gegenüber Erythromycin (15 μg) waren alle <em>Acholeplasma</em>-Isolate empfindlich und alle <em>Mycoplasma</em>-Isolate resistent. Gegenüber Streptomycin (10 μg) erwiesen sich die beiden <em>M. subdolum</em> Stämme als resistent; <em>M. equigentialium</em> war wie die Acholeplasmen empfindlich, wenn auch in wesentlich geringerem Maße. Die Bedeutung der Mykoplasmen im Genitaltrakt der Stuten wird diskutiert.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"246 2","pages":"Pages 228-235"},"PeriodicalIF":0.0,"publicationDate":"1980-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80116-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88159416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Listeriosis in Humans and Animals in the Netherlands (1958–1977)","authors":"E.H. Kampelmacher ,&nbsp;Lucretia M. van Noorle Jansen","doi":"10.1016/S0172-5599(80)80115-5","DOIUrl":"10.1016/S0172-5599(80)80115-5","url":null,"abstract":"<div><p>During the past twenty years 793 strains of <em>Listeria monocytogenes</em> were isolated from human beings in the Netherlands; of these 193 were taken from neonates and babies up to two months old. Isolations from adults came from patients (242) as well as clinically healthy people (358). Septicaemia appeared to occur to an equal extent in men and women (20 and 29 respectively), whereas meningitis was found more often in men than in women (50 and 22 respectively). A clear predisposition for <em>L. m.</em> develops after administration of immunosuppressive treatment and also in cases of liver disorders. Among veterinary surgeons listeriosis has been observed as an occupational disease. Isolations from animals have shown that <em>L.m.</em> may cause infections in every species of warm-blooded animal. Next to meningitis and abortus chronic and atypcial symptoms of the disease may be observed in animals. Epizootic spread of the disease has hardly ever been observed in the Netherlands.</p><p>In clinically healthy humans and animals both the haemolytic and the non-haemolytic type of <em>L.m.</em> have been isolated from feces; as regards the latter type it is very questionable whether it has any pathogenic significance. When inoculating 10 days old hen's eggs the haemolytic strains will kill all embryo's within 4 days whereas all embryo's inoculated with non-haemolytic strains will survive. Both types of strains have also been isolated from waste- and surface waters. As regards epidemiological and epizootiological aspects the conclusion is warranted that continued research will be needed to get a clear picture of the course of infection of <em>L. m.</em> When isolating strains from contaminated material the so-called cold enrichment incubation at 4 °C continues to be of great value; in the course of our experiments the nalidixic-acid-trypaflavine serum agar proved to be a very good selective medium.</p><p>A number of stable biochemical reactions of <em>L. m.</em> are rather characteristic (salicine +, galactose −) but provide no clue to distinguish between haemolytic and non-haemolytic strains.</p></div><div><p>Während der letzten 20 Jahre wurden 793 Stämme <em>Listeria monocytogenes</em> von Personen in den Niederlanden isoliert. 193 stammten von Neugeborenen und Säuglingen bis zu 2 Monaten alt; 242 Stämme wurden von erwachsenen Patienten und 358 von klinisch gesunden Personen isoliert. Septikämie war bei Männern und Frauen 20 bzw. 29 mal aufgetreten, dagegen war eine Meningitis häufiger bei Männern als bei Frauen (50 bzw. 22mal).Die Verabfolgung einer immunsuppressiven Behandlung und bestehende Leberschäden bedeuten eine klare Prädisposition für die <em>L. m.</em> Die Listeriose hat als eine Berufskrankheit unter den Veterinär-Chirurgen zu gelten. Auf Grund von Isolierungen der <em>L. m.</em> bei Tieren konnte gezeigt werden, daß die <em>L. m.</em> jede Spezies von Warmblütern infizieren kann. Außer Meningitis können chronischer Abort und at","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"246 2","pages":"Pages 211-227"},"PeriodicalIF":0.0,"publicationDate":"1980-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80115-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89746530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zur Charakterisierung der Mikroflora des Dünndarms","authors":"Hannelore Bernhardt ,&nbsp;M. Knoke","doi":"10.1016/S0172-5599(80)80076-9","DOIUrl":"10.1016/S0172-5599(80)80076-9","url":null,"abstract":"<div><p>Normal and abnormal microflora of the upper small intestine was studied in 356 patients. Low counts are characteristic of normal microbial colonization (eubiosis), changes in quality and/or quantity are pathological (dysbiosis). The latter status is described as overgrowth syndrome. We found some types of dysbiosis. Prevalent was type Dys₁ with the highest counts and the greatest variety of bacteria and yeasts. In contrast to this, type Dys₂ showed higher germ counts of only one genus like coliforms (Dys₂ Coli), streptococci (Dys₂ Str.), lactobacilli (Dys₂ L.) or yeasts (Dys₂ Y.). In dysbiosis, we frequently saw bifidobacterium and bacteroides. Simultaneous sampling from stomach, duodenum, and jejunum indicated different modes of colonization of these parts (oral or fecal type).</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"246 3","pages":"Pages 379-392"},"PeriodicalIF":0.0,"publicationDate":"1980-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80076-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77197315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Action of Three Species of Propionibacteria (P. granulosum, P. acnes, P. avidum) on Experimental Tumor Systems in Mice","authors":"Waldemar Roszkowski ,&nbsp;Miroslaw Kobus ,&nbsp;Miroslaw Luczak ,&nbsp;Hong Lioe Ko ,&nbsp;Stanislaw Szmigielski ,&nbsp;Bozena Laskowska ,&nbsp;Gerhard Pulverer ,&nbsp;Janusz Jeljaszewicz","doi":"10.1016/S0172-5599(80)80078-2","DOIUrl":"10.1016/S0172-5599(80)80078-2","url":null,"abstract":"<div><p>The antitumor activity of three differenr species of Propionibacteria <em>(P. granulosum, P. avidum</em> and <em>P. acnes)</em> against murine <em>Sarcoma 180</em> and <em>Moloney-Sarcoma-Virus</em>-induced tumor (MSV tumor) was investigated.</p><p>All three Propionibacteria injected intraperitoneally or intratumorally in multiple doses of 1 mg per mouse appeared to be effective regarding the retardation of growth and the stimulation of regression of <em>Sarcoma 180</em> in CFW mice. Moreover, the application of Propionibacteria significantly prolonged the survival of <em>Sarcoma-180</em>-bearing mice. No significant differences were found in antitumor effect against <em>Sarcoma 180</em> between <em>P. granulosum, P. avidum</em> and <em>P. acnes</em>, but in general, intratumoral injections of all three strains were more effective than intraperitoneal applications.</p><p>All three strains of Propionibacteria were equally ineffective regarding the retardation of growth and the stimulation of regression of MSV <em>tumors</em> in adult NMRI mice, as assessed after intraperitoneal injections of <em>P. granulosum, P. avidum</em> or <em>P. acnes</em> (1 mg per mouse) 3 days prior to inoculation of virus particles.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"246 3","pages":"Pages 405-414"},"PeriodicalIF":0.0,"publicationDate":"1980-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80078-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74259350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colicin Typing of Shigella sonnei by Means of Specific Indicator Strains. An Enlarged Scheme","authors":"Vladimír Horák","doi":"10.1016/S0172-5599(80)80112-X","DOIUrl":"10.1016/S0172-5599(80)80112-X","url":null,"abstract":"<div><p>The scheme of colicin typing of <em>Shigella sonnei</em> includes 26 colicin types. Thirty-three indicator strains were used for typing and 22 of them were prepared by conjugational transfer of col factors from shigellae on recipient strain of <em>E. coli</em> K 13 HfrR nal^r or <em>E. coli</em> K 12 ROW. Immune strains of <em>Sh. sonnei</em> or resistant mutants of <em>E.coli</em> K 12 ROW were used as indicator strains only sporadically.</p><p>Zusammenfassung</p><p>Das Colicin-Typisierungsschema von Sh. sonnei umfaßt 26 Colicintypen. Für die Typisierung wurden 33 Indikatorstämme verwandt, wovon 22 durch Konjugationsübertragung von Col-Faktoren der Shigellen auf Empfängerstämme von E. coli K 13 HfrR nal^r oder E.coli K 12 ROW prepariert wurden. Immunstämme von Sh. sonnei oder resistente Mutanten von E. coli K 12 ROW wurden nur vereinzelt als Indikatorstämme verwandt.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"246 2","pages":"Pages 191-196"},"PeriodicalIF":0.0,"publicationDate":"1980-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80112-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72618810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}