Nachweis von Leptospiren-Antikörpern in Seren immunisierter Kaninchen mit Hilfe des indirekten Immunfluoreszenztestes

Abstract

Antibodies of Leptospirae were demonstrated in the sera of immunized rabbits by the indirect immunofluorescence test, only after a suitable dilution of the “anti-rabbit-gammaglobulin fluorescin conjugated” was carried out in a cross reference titration (“chessboard method”). A distinct crossreaction was observed when anti-icterohaemorrhagiae — and anti-bratislava-immunsera reacted with several serotypes out of the “interrogans complex”. But only a weak crossreaction was found when both sera reacted with the serotyp patoc from the “biflexa complex”. The evaluation of the indirect immunofluorescence test was complicated by a streaky background of fluorescence. The agargeldiffusion tests (Ouchterlony) exhibited four, three and two precipitation bands according to the combination of antigens and immunsera.