锐利古根2号的t蛋白质介绍:分离性免疫免疫制剂并分离T4/24病毒净化组

K.-H. Schmidt , O. Kühnemund, W. Köhler
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引用次数: 3

摘要

对化脓性链球菌T4/24型标准株中t -复合物抗原的一致性和多样性进行了研究。采用deae -纤维素离子交换层析纯化4、24、26、28、29、46、48、60型色氨酸t提取物。采用免疫吸附层析法在AH-sepharose连接的t4抗原上分离到抗t4抗体。纯化后的t4抗原在sds电泳中显示出与前面描述的t1抗原相似的多分子大小结构。4、24、29和46型t抗原的比较血清学研究揭示了Ouchterlony试验中抗t抗体的同一性反应。26型、28型、48型和60型提取物与抗t - 4抗体均无沉淀,而28型和48型提取物与相关抗血清(抗t28和抗t48等)发生交叉反应,明显是由于两种抗原制剂中均含有微量R-28抗原所致。4型、24型、29型、46型、48型和60型被抗t - 4抗体凝集。t4抗原可抑制该反应。实验中使用的26型和28型菌株不含t4抗原。用免疫层析法制备特异性抗体进行凝集和免疫沉淀反应,证实了在4、24、29、46、48和60型中存在常见的t4抗原决定因子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
T-Proteine des Streptococcus pyogenes II. Mitteilung: Präparation spezifischer Immunadsorbentien zur Isolierung von T-Antikörpern und Untersuchungen der T4/24… Agglutinationsgruppe

The T4-antigen(s) in Streptococcus pyogenes standard type strains of the T4/24… pattern was (were) investigated in regard to the uniformity or diversity of the antigen(s) in this T-complex. Tryptic T-extracts of types 4, 24, 26, 28, 29, 46, 48, 60 were purified by ion exchange chromatography on DEAE-cellulose. Anti-T4-antibodies were isolated by immunoadsorption chromatography on AH-sepharose linked T4-antigen.

Purified T4-antigen showed in SDS-electrophoresis a similar multiple molecular size structure as T1-antigen described earlier. Comparative serological studies of T-antigens of types 4, 24, 29 and 46 revealed reactions of identity to anti-T 4-antibodies in Ouchterlony tests. Extracts of types 26, 28, 48 and 60 did not precipitate with anti-T 4-antibodies, but types 28 and 48 showed crossreaction to the relevant antisera (anti-T28 and anti-T48, resp.) obviously caused by traces of R-28 antigen in both antigen preparations. Strains of the types 4, 24, 29, 46, 48 and 60 were agglutinated by anti-T 4-antibodies. The reaction could be inhibited by T4-antigen. The strains of types 26 and 28 used in our experiments did not contain T4-antigen.

Agglutination as well as immunoprecipitation reactions with specific antibodies prepared by immunochromatography proved the existence of common T4-antigenic determinants in types 4, 24, 29, 46, 48 and 60.

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