Rafael Ponikvar MD, Joze Drinovec MD, PhD, Janez Varl MD, Andrej Bren MD, Radoslav Kveder MD
{"title":"Adsorption and desorption of proteins on plasmafilter membrane and its effect on macromolecular sieving coefficient","authors":"Rafael Ponikvar MD, Joze Drinovec MD, PhD, Janez Varl MD, Andrej Bren MD, Radoslav Kveder MD","doi":"10.1016/S0278-6222(87)80034-7","DOIUrl":"10.1016/S0278-6222(87)80034-7","url":null,"abstract":"<div><p>The purpose of this study was to find out the influence of adsorption and desorption of plasma proteins on the sieving coefficient during membrane plasma exchange. Adsorption and desorption of plasma proteins on plasmafilter membrane was an important feature in the study. It was more intensive with proteins with higher molecular weight (for IgG, α-2 macroglobulin, and IgM) and was not important with albumin. Sieving coefficients for IgG, α-2 macroglobulin, and IgM were unexpectedly high, while that of albumin remained within normal limits.</p></div>","PeriodicalId":101030,"journal":{"name":"Plasma Therapy and Transfusion Technology","volume":"8 4","pages":"Pages 295-297"},"PeriodicalIF":0.0,"publicationDate":"1987-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0278-6222(87)80034-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88304753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Böck, Ch. Krause, M. Wagner, M.U. Heim, S. Braun, U. Pachmann, W. Mempel
{"title":"Transfusion of leukocyte-depleted red cell concentrates and whole blood with a new bedside filter system","authors":"M. Böck, Ch. Krause, M. Wagner, M.U. Heim, S. Braun, U. Pachmann, W. Mempel","doi":"10.1016/S0278-6222(87)80036-0","DOIUrl":"10.1016/S0278-6222(87)80036-0","url":null,"abstract":"<div><p>In the past, several techniques have been established for preparing leukocytepoor blood. However, all these techniques are time consuming and require the facilities of a blood bank. We have tested a newly developed filter system (Sepacell R 500A) for direct bedside filtration that significantly reduces this expenditure. The filter retains leukocytes and platelets by about 99.3% and 94.2%, respectively. It has no effect on filtered red cells. Plasma sodium, calcium and potassium concentrations, and pH are not changed. Clinically, no side effects have been observed.</p></div>","PeriodicalId":101030,"journal":{"name":"Plasma Therapy and Transfusion Technology","volume":"8 4","pages":"Pages 302-306"},"PeriodicalIF":0.0,"publicationDate":"1987-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0278-6222(87)80036-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77716478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Janos G. Kadar , Peter Späth , Andrzej Gaczkowski , Kurt Oette , Helmut Borberg
{"title":"Biocompatibility studies on a clinically well-tolerated extracorporeal system","authors":"Janos G. Kadar , Peter Späth , Andrzej Gaczkowski , Kurt Oette , Helmut Borberg","doi":"10.1016/S0278-6222(87)80037-2","DOIUrl":"10.1016/S0278-6222(87)80037-2","url":null,"abstract":"<div><p>A consistent repetitive change in the peripheral PMNL and monocyte counts during the clinically well-tolerated removal of LDL by SIA, using centrifugal BCS and antihuman apolipoprotein-B-antibody Sepharose 4B columns, has been observed. Also, a subsequent elevation of PMN-derived elastase-alpha 1 inhibitor-complex was found. Preliminary evidence and comparable laboratory data from other extracorporeal systems that are not biologically inert, suggest that plasma protein cascades may also be involved in the pathogenesis of these phenomena. The lack of potential clinical symptoms, in contrast to the laboratory observations, demonstrate that activation of plasma protein cascades that are not clinically relevant may occur. The long-term pathophysiologic consequences for patients treated regularly by SIA remain to be elucidated. Also, further systematic laboratory work is needed for a better understanding of the observations.</p></div>","PeriodicalId":101030,"journal":{"name":"Plasma Therapy and Transfusion Technology","volume":"8 4","pages":"Pages 307-318"},"PeriodicalIF":0.0,"publicationDate":"1987-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0278-6222(87)80037-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77375976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Case studies in compartment system analyses of biosystems","authors":"Dietmar P.F. Möller, Thomas Sikora","doi":"10.1016/S0278-6222(87)80033-5","DOIUrl":"10.1016/S0278-6222(87)80033-5","url":null,"abstract":"","PeriodicalId":101030,"journal":{"name":"Plasma Therapy and Transfusion Technology","volume":"8 4","pages":"Pages 290-294"},"PeriodicalIF":0.0,"publicationDate":"1987-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0278-6222(87)80033-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73563084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dietrich W. Beelen MD, Klaus Quabeck MD, Annette Schmidt-Weinmar DVM, Ursula Ryschka, Hans Grosse-Wilde MD (Prof.), Ullrich Graeven MD, Herbert Saver MD, Werner Luboldt MD, Ulrich W. Schaefer MD (Prof.), Carl G. Schmidt MD (Prof.)
{"title":"Separation of red blood cell-depleted mononuclear marrow cells by continuous-flow centrifugation using the IBM 2997 blood cell separator—Results of a preclinical study in a dog model","authors":"Dietrich W. Beelen MD, Klaus Quabeck MD, Annette Schmidt-Weinmar DVM, Ursula Ryschka, Hans Grosse-Wilde MD (Prof.), Ullrich Graeven MD, Herbert Saver MD, Werner Luboldt MD, Ulrich W. Schaefer MD (Prof.), Carl G. Schmidt MD (Prof.)","doi":"10.1016/S0278-6222(87)80042-6","DOIUrl":"10.1016/S0278-6222(87)80042-6","url":null,"abstract":"<div><p>As an alternative technique to density-gradient separation, we studied the preparation of red blood cell (RBC)-depleted marrow mononuclear cells (MNC) by continuous-flow centrifugation (CFC) using the IBM 2997 cell separator in a dog model. Mean recoveries of nucleated and mononuclear cells obtained by this separation technique were 43.1% and <em>77.7%</em>, respectively, and the mean residual RBC volume was 1.8 mL (1.2% of the RBC volume prior to separation). A mean of 91.4% of marrow progenitor cells were regained in the RBC-depleted fraction. Transplantation of the separated marrow MNC into seven lethally irradiated beagle littermates resulted in complete and durable hematopoietic chimerism. Our results indicate that separation of marrow MNC using an IBM 2997 cell separator is an effective and technically uncomplicated alternative to gradient medium separation.</p></div>","PeriodicalId":101030,"journal":{"name":"Plasma Therapy and Transfusion Technology","volume":"8 4","pages":"Pages 351-354"},"PeriodicalIF":0.0,"publicationDate":"1987-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0278-6222(87)80042-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81757049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Derwood H. Pamphilon MBBS, MRCPath, Valerie Godwin, Michael J. Gunstone
{"title":"The use of the Fenwal CS3000 cell separator to purify a mononuclear cell fraction from pooled buffy coats","authors":"Derwood H. Pamphilon MBBS, MRCPath, Valerie Godwin, Michael J. Gunstone","doi":"10.1016/S0278-6222(87)80046-3","DOIUrl":"10.1016/S0278-6222(87)80046-3","url":null,"abstract":"<div><p>Using a modified plateletpheresis program on the Fenwal CS3000, we have studied the recovery of mononuclear cells and clonogenic potential from pooled buffy coats. Recovery of mononuclear cells and CFU-GM was 90% and 67%, respectively. The final volume could easily be adjusted to 100 mL, and erythrocyte contamination was low (2.3%). This method should be readily applicable to bone marrow processing to achieve efficient and rapid separation for subsequent reinfusion, cryopreservation, and treatment with monoclonal antibodies.</p></div>","PeriodicalId":101030,"journal":{"name":"Plasma Therapy and Transfusion Technology","volume":"8 4","pages":"Pages 368-370"},"PeriodicalIF":0.0,"publicationDate":"1987-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0278-6222(87)80046-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88511013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Reiffers , S. Castaigne , H. Tilly , E. Lepage , G. Leverger , P. Henon , L. Douay
{"title":"Hematopoietic reconstitution after autologous blood stem cell transplantation: A report of 46 cases","authors":"J. Reiffers , S. Castaigne , H. Tilly , E. Lepage , G. Leverger , P. Henon , L. Douay","doi":"10.1016/S0278-6222(87)80044-X","DOIUrl":"10.1016/S0278-6222(87)80044-X","url":null,"abstract":"<div><p>Hematopoietic reconstitution using autologous blood stem cells after high-dose chemoradiotherapy has been achieved successfully in patients with acute leukemias,<sup>1–4</sup> non-Hodgkin's lymphomas,<sup>5–6</sup> or solid tumors.<sup>7</sup> Some patients, however, had incomplete hematopoietic engraftment, which may be due to insufficient numbers of progenitors infused or to the recurrence of leukemic cells. <sup>1,8,9</sup> Thus, the question arises: Is there a safe minimum number of blood progenitors that will produce complete hematopoietic reconstitution following supralethal therapy? To answer this question, we reviewed the data from 46 patients who have undergone autologus blood stem cell transplantation (ABSCT) in six different centers in France.</p></div>","PeriodicalId":101030,"journal":{"name":"Plasma Therapy and Transfusion Technology","volume":"8 4","pages":"Pages 360-362"},"PeriodicalIF":0.0,"publicationDate":"1987-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0278-6222(87)80044-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77943598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hans von Baeyer, Frank Kochinke, Ingo Schwaner, Rainer Schwerdtfeger, Wolfgang Schwarztkopff
{"title":"Fundamentals and application of differential filtration","authors":"Hans von Baeyer, Frank Kochinke, Ingo Schwaner, Rainer Schwerdtfeger, Wolfgang Schwarztkopff","doi":"10.1016/S0278-6222(87)80031-1","DOIUrl":"10.1016/S0278-6222(87)80031-1","url":null,"abstract":"<div><p>The fractionation of macrosolute mixtures into classes of graded molecular sizes is the most difficult and, at the same time, most desirable membrane moderated process, according to W.F. Blatt. The major problems originate from concentration polarization, pore plugging, and membrane fouling. Up until now there has been no comprehensive theory that describes these filtration effects satisfactorily. Thus, all accomplishments of differential filtration techniques are based mainly on empirical knowledge.</p><p>According to concentration polarization phenomena, increasing membrane wall concentrations of rejected macromolecules superimpose an additional membrane resistance. This dynamically formed “hybrid membrane” may govern the filtration process, exerting a dual effect upon hydraulic and solute permeability.</p><p>Experience obtained by studying the physics of differential filtration guided us to develop a system using currently available artificial membranes for clinical application. This system uses membrane modules with a nominal cutoff of 0.6 mega Dalton, which enables the separation of macroproteins out of the spectrum of plasma proteins. Thus, this system can be applied for the hemapheretic treatment of hypercholesterolemia with excessively high levels of low density lipoprotein (LDL).</p><p>This overview contains some basic principles of differential filtration and the data of its clinical use. Clinical observations as to regression of atherosclerotic lesions are not included, but are intended for separate publication.</p></div>","PeriodicalId":101030,"journal":{"name":"Plasma Therapy and Transfusion Technology","volume":"8 4","pages":"Pages 273-282"},"PeriodicalIF":0.0,"publicationDate":"1987-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0278-6222(87)80031-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77987868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}