Derwood H. Pamphilon MBBS, MRCPath, Valerie Godwin, Michael J. Gunstone
{"title":"使用Fenwal CS3000细胞分离器从混合的褐皮中纯化单个核细胞部分","authors":"Derwood H. Pamphilon MBBS, MRCPath, Valerie Godwin, Michael J. Gunstone","doi":"10.1016/S0278-6222(87)80046-3","DOIUrl":null,"url":null,"abstract":"<div><p>Using a modified plateletpheresis program on the Fenwal CS3000, we have studied the recovery of mononuclear cells and clonogenic potential from pooled buffy coats. Recovery of mononuclear cells and CFU-GM was 90% and 67%, respectively. The final volume could easily be adjusted to 100 mL, and erythrocyte contamination was low (2.3%). This method should be readily applicable to bone marrow processing to achieve efficient and rapid separation for subsequent reinfusion, cryopreservation, and treatment with monoclonal antibodies.</p></div>","PeriodicalId":101030,"journal":{"name":"Plasma Therapy and Transfusion Technology","volume":"8 4","pages":"Pages 368-370"},"PeriodicalIF":0.0000,"publicationDate":"1987-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0278-6222(87)80046-3","citationCount":"0","resultStr":"{\"title\":\"The use of the Fenwal CS3000 cell separator to purify a mononuclear cell fraction from pooled buffy coats\",\"authors\":\"Derwood H. Pamphilon MBBS, MRCPath, Valerie Godwin, Michael J. Gunstone\",\"doi\":\"10.1016/S0278-6222(87)80046-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Using a modified plateletpheresis program on the Fenwal CS3000, we have studied the recovery of mononuclear cells and clonogenic potential from pooled buffy coats. Recovery of mononuclear cells and CFU-GM was 90% and 67%, respectively. The final volume could easily be adjusted to 100 mL, and erythrocyte contamination was low (2.3%). This method should be readily applicable to bone marrow processing to achieve efficient and rapid separation for subsequent reinfusion, cryopreservation, and treatment with monoclonal antibodies.</p></div>\",\"PeriodicalId\":101030,\"journal\":{\"name\":\"Plasma Therapy and Transfusion Technology\",\"volume\":\"8 4\",\"pages\":\"Pages 368-370\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1987-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0278-6222(87)80046-3\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plasma Therapy and Transfusion Technology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0278622287800463\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plasma Therapy and Transfusion Technology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0278622287800463","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The use of the Fenwal CS3000 cell separator to purify a mononuclear cell fraction from pooled buffy coats
Using a modified plateletpheresis program on the Fenwal CS3000, we have studied the recovery of mononuclear cells and clonogenic potential from pooled buffy coats. Recovery of mononuclear cells and CFU-GM was 90% and 67%, respectively. The final volume could easily be adjusted to 100 mL, and erythrocyte contamination was low (2.3%). This method should be readily applicable to bone marrow processing to achieve efficient and rapid separation for subsequent reinfusion, cryopreservation, and treatment with monoclonal antibodies.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
