Separation of red blood cell-depleted mononuclear marrow cells by continuous-flow centrifugation using the IBM 2997 blood cell separator—Results of a preclinical study in a dog model

Plasma Therapy and Transfusion Technology Pub Date : 1987-12-01 DOI:10.1016/S0278-6222(87)80042-6

Dietrich W. Beelen MD, Klaus Quabeck MD, Annette Schmidt-Weinmar DVM, Ursula Ryschka, Hans Grosse-Wilde MD (Prof.), Ullrich Graeven MD, Herbert Saver MD, Werner Luboldt MD, Ulrich W. Schaefer MD (Prof.), Carl G. Schmidt MD (Prof.)

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引用次数: 2

Abstract

As an alternative technique to density-gradient separation, we studied the preparation of red blood cell (RBC)-depleted marrow mononuclear cells (MNC) by continuous-flow centrifugation (CFC) using the IBM 2997 cell separator in a dog model. Mean recoveries of nucleated and mononuclear cells obtained by this separation technique were 43.1% and 77.7%, respectively, and the mean residual RBC volume was 1.8 mL (1.2% of the RBC volume prior to separation). A mean of 91.4% of marrow progenitor cells were regained in the RBC-depleted fraction. Transplantation of the separated marrow MNC into seven lethally irradiated beagle littermates resulted in complete and durable hematopoietic chimerism. Our results indicate that separation of marrow MNC using an IBM 2997 cell separator is an effective and technically uncomplicated alternative to gradient medium separation.

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使用IBM 2997血细胞分离器连续离心分离红细胞耗尽的单核骨髓细胞-犬模型临床前研究的结果

作为密度梯度分离的替代技术，我们研究了在狗模型中使用IBM 2997细胞分离器连续流式离心(CFC)制备红细胞(RBC)耗尽的骨髓单个核细胞(MNC)。有核细胞和单核细胞的平均回收率分别为43.1%和77.7%，平均残余红细胞体积为1.8 mL(分离前红细胞体积的1.2%)。在红细胞耗尽的部分中，平均91.4%的骨髓祖细胞被恢复。将分离的骨髓移植到7只受致命辐射的比格犬幼崽中，产生了完整和持久的造血嵌合。我们的研究结果表明，使用IBM 2997细胞分离器分离骨髓MNC是一种有效的、技术上简单的梯度培养基分离方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Plasma Therapy and Transfusion Technology

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