Mechanobiology in Medicine最新文献_第5页

Extracellular matrix stiffness modulates the mechanophenotypes and focal adhesions of colon cancer cells leading to their invasions via YAP1 细胞外基质硬度通过 YAP1 调节结肠癌细胞的机械表型和病灶粘附，从而导致其侵袭

Mechanobiology in Medicine Pub Date : 2024-03-19 DOI: 10.1016/j.mbm.2024.100062

Kaide Xia , Wenhui Hu , Yun Wang , Jin Chen , Zuquan Hu , Chenyi An , Pu Xu , Lijing Teng , Jieheng Wu , Lina Liu , Sichao Zhang , Jinhua Long , Zhu Zeng

{"title":"Extracellular matrix stiffness modulates the mechanophenotypes and focal adhesions of colon cancer cells leading to their invasions via YAP1","authors":"Kaide Xia , Wenhui Hu , Yun Wang , Jin Chen , Zuquan Hu , Chenyi An , Pu Xu , Lijing Teng , Jieheng Wu , Lina Liu , Sichao Zhang , Jinhua Long , Zhu Zeng","doi":"10.1016/j.mbm.2024.100062","DOIUrl":"10.1016/j.mbm.2024.100062","url":null,"abstract":"<div><p>Distal metastasis is the main cause of clinical treatment failure in patients with colon cancer. It is now known that the invasion and metastasis of cancer cells is precisely regulated by chemical and physical factors <em>in vivo</em>. However, the role of extracellular matrix (ECM) stiffness in colon cancer cell (CCCs) invasion and metastasis remains unclear. Here, bioinformatical analysis suggested that a high expression level of yes associated protein 1 (YAP1) was significantly associated with metastasis and poor prognosis in colon cancer patients. We further investigated the effects of polyacrylamide hydrogels with different stiffnesses (3, 20, and 38 kPa), which were simulated as ECM, on the mechanophenotype (F-actin cytoskeleton organization, electrophoretic rate, membrane fluidity, and Young's modulus) of CCCs. The results showed that a stiffer ECM could induce the maturation of focal adhesions and formation of stress fibers in CCCs, regulate their mechanophenotypes, and promote cell motility. We also demonstrated that the expression levels of YAP1 and paxillin were positively correlated in patients with colon cancer. YAP1 knockdown reduces paxillin clustering and cell motility and alters the cellular mechanophenotypes of CCCs. This is of great significance for an in-depth understanding of the invasion and metastatic mechanisms of colon cancer and for the optimization of clinical therapy from the perspective of mechanobiology.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":"2 2","pages":"Article 100062"},"PeriodicalIF":0.0,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949907024000251/pdfft?md5=8225712552eb8e5633ae7b59efe64ce1&pid=1-s2.0-S2949907024000251-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140280551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Bead-based microfluidic platforms for multiplex and ultrasensitive immunoassays in clinical diagnosis and treatment 基于微珠的微流控平台，用于临床诊断和治疗中的多重和超灵敏免疫测定

Mechanobiology in Medicine Pub Date : 2024-03-16 DOI: 10.1016/j.mbm.2024.100063

Xiaoxia Fang, Yiwen Yang, Heni Wang, Hong Xu

引用次数: 0

Regulatory role of interfacial adhesion and mechanical microenvironments in microbe-host interactions 界面粘附和机械微环境在微生物-宿主相互作用中的调节作用

Mechanobiology in Medicine Pub Date : 2024-03-01 DOI: 10.1016/j.mbm.2024.100060

Yuting Feng, Jianyong Huang

{"title":"Regulatory role of interfacial adhesion and mechanical microenvironments in microbe-host interactions","authors":"Yuting Feng, Jianyong Huang","doi":"10.1016/j.mbm.2024.100060","DOIUrl":"10.1016/j.mbm.2024.100060","url":null,"abstract":"<div><p>A recent study published in <em>Nature Communications</em> showed that essential modulatory roles of interfacial adhesion and mechanical microenvironments such as geometric constraints and extracellular matrix stiffness, in microbe-host cell interactions. This study utilized single-cell force spectroscopy and RNA sequencing to gain insight into the intrinsic mechanisms by which the mechanical microenvironment regulates bacterial-host interactions and therefore reveal potential interventions against bacterial invasion. Meanwhile, the adhesion forces involved in the bacterial–host interactions were recognized as a new indicator for assessing the extent of bacterial infection. Taken together, these findings demonstrate that interfacial adhesion forces and mechanical microenvironments play a dominant role in modulating functions and behaviors of microorganisms and host cells, which also provide a mechanobiology-inspired idea for the development of subsequent drug-resistant antimicrobials and broad-spectrum antiviral drugs.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":"2 2","pages":"Article 100060"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949907024000238/pdfft?md5=5a9dc4292dc1bffe61407088d51445eb&pid=1-s2.0-S2949907024000238-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140084375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Rescuing SERCA2 pump deficiency: A novel approach to improve bone mechano-responsiveness in type 2 diabetes 挽救 SERCA2 泵缺陷：改善 2 型糖尿病患者骨骼机械反应性的新方法

Mechanobiology in Medicine Pub Date : 2024-02-24 DOI: 10.1016/j.mbm.2024.100047

Zhifeng Yu , X. Edward Guo

{"title":"Rescuing SERCA2 pump deficiency: A novel approach to improve bone mechano-responsiveness in type 2 diabetes","authors":"Zhifeng Yu , X. Edward Guo","doi":"10.1016/j.mbm.2024.100047","DOIUrl":"https://doi.org/10.1016/j.mbm.2024.100047","url":null,"abstract":"<div><p>A recent study published in <em>Nature Communications</em> demonstrated that restoring SERCA2 pump deficiency can enhance bone mechano-responsiveness in type 2 diabetes (T2D) by modulating osteocyte calcium dynamics. The findings revealed that in T2D mice, the ability of the bone to respond to mechanical stress is compromised, primarily due to attenuated calcium oscillatory dynamics within osteocytes rather than in osteoblasts or osteoclasts. The underlying mechanism of this reduction in bone mechano-responsiveness in T2D was identified as a specific decrease in osteocytic SERCA2 expression mediated by PPARα. Additionally, mice overexpressing SERCA2 in osteocytes exhibited reduced deterioration of bone mechano-responsiveness induced by T2D. Collectively, this study provides mechanistic insights into T2D-induced deterioration in bone mechano-responsiveness and identifies a promising therapeutic approach to counteract T2D-associated fragility fractures.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":"2 2","pages":"Article 100047"},"PeriodicalIF":0.0,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S294990702400010X/pdfft?md5=673fabd07f7fc63f32f1485c817369bc&pid=1-s2.0-S294990702400010X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140112983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of matrix stiffness on immune cells in bone biology 骨生物学中基质硬度对免疫细胞的影响

Mechanobiology in Medicine Pub Date : 2024-02-22 DOI: 10.1016/j.mbm.2024.100046

Ting Jiang , Meng-Ting Zheng , Ruo-Mei Li , Ning-Juan Ouyang

{"title":"The effects of matrix stiffness on immune cells in bone biology","authors":"Ting Jiang , Meng-Ting Zheng , Ruo-Mei Li , Ning-Juan Ouyang","doi":"10.1016/j.mbm.2024.100046","DOIUrl":"10.1016/j.mbm.2024.100046","url":null,"abstract":"<div><p>Bone and immune cells typically inhabit the same microenvironment and engage in mutual interactions to collectively execute the functions of the “osteoimmune system.” Establishing a harmonized and enduring osteoimmune system significantly enhances bone regeneration, necessitating the maintenance of bone and immune homeostasis. Recently, mechanobiology has garnered increasing interest in bone tissue engineering, with matrix stiffness emerging as a crucial parameter that has been extensively investigated. The effect of matrix stiffness on bone homeostasis remains relatively clear. Soft substrates tend to significantly affect the chondrogenic differentiation of bone marrow mesenchymal stem cells, whereas increasing matrix stiffness is advantageous for osteogenic differentiation. Increased stiffness increases osteoclast differentiation and activity. Additionally, there is increasing emphasis on immune homeostasis, which necessitates dynamic communication between immune cells. Immune cells are crucial in initiating bone regeneration and driving early inflammatory responses. Functional changes induced by matrix stiffness are pivotal for determining the outcomes of engineered tissue mimics. However, inconsistent and incomparable findings regarding the responses of different immune cells to matrix stiffness can be perplexing owing to variations in the stiffness range, measurement methods, and other factors. Therefore, this study aimed to provide a comprehensive review of the specific effects of matrix stiffness on diverse immune cells, with a particular focus on its implications for bone regeneration, which would offer theoretical insights into the treatment of large segmental bony defects and assist in the clinical development of new engineering strategies.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":"2 2","pages":"Article 100046"},"PeriodicalIF":0.0,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949907024000093/pdfft?md5=32830fd5ccb0440aa455483d1f52a402&pid=1-s2.0-S2949907024000093-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140465958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanics of serotonin-producing human entero-endocrine cells 分泌羟色胺的人类肠内分泌细胞的机理

Mechanobiology in Medicine Pub Date : 2024-02-08 DOI: 10.1016/j.mbm.2024.100044

Tom M.J. Evers , Joep Beumer , Hans Clevers , Alireza Mashaghi

引用次数: 0

Mechanotransduction in distinct F-actin architectures: a novel molecular tension sensor revealing cellular mechanical anisotropy 不同 F-肌动蛋白结构中的机械传导：揭示细胞机械各向异性的新型分子张力传感器

Mechanobiology in Medicine Pub Date : 2024-02-06 DOI: 10.1016/j.mbm.2024.100045

Ting Liang, Bin Li

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Mechanotransduction in subchondral bone microenvironment and targeted interventions for osteoarthritis 软骨下骨微环境中的机制传导和骨关节炎的靶向干预措施

Mechanobiology in Medicine Pub Date : 2024-02-05 DOI: 10.1016/j.mbm.2024.100043

Rui Feng , Wenhui Hu , Yuheng Li , Xuan Yao , Jianmei Li , Xiaoming Li , Jing Zhang , Yu Wu , Fei Kang , Shiwu Dong

{"title":"Mechanotransduction in subchondral bone microenvironment and targeted interventions for osteoarthritis","authors":"Rui Feng , Wenhui Hu , Yuheng Li , Xuan Yao , Jianmei Li , Xiaoming Li , Jing Zhang , Yu Wu , Fei Kang , Shiwu Dong","doi":"10.1016/j.mbm.2024.100043","DOIUrl":"10.1016/j.mbm.2024.100043","url":null,"abstract":"<div><p>Osteoarthritis (OA) is a progressive degenerative joint sickness related with mechanics, obesity, ageing, <em>etc</em>., mainly characterized by cartilage degeneration, subchondral bone damage and synovium inflammation. Coordinated mechanical absorption and conduction of the joint play significant roles in the prevalence and development of OA. Subchondral bone is generally considered a load-burdening tissue where mechanosensitive cells are resident, including osteocytes, osteoblast lineage cells, and osteoclast lineage cells (especially less concerned in mechanical studies). Mechano-signaling imbalances affect complicated cellular events and disorders of subchondral bone homeostasis. This paper will focus on the significance of mechanical force as the pathogenesis, involvement of various mechanical force patterns in mechanosensitive cells, and mechanobiology research of loading devices <em>in vitro</em> and <em>in vivo</em>, which are further discussed. Additionally, various mechanosensing structures (<em>e.g</em>., transient receptor potential channels, gap junctions, primary cilia, podosome-associated complexes, extracellular vesicles) and mechanotransduction signaling pathways (<em>e.g</em>., Ca<sup>2+</sup> signaling, Wnt/β-catenin, RhoA GTPase, focal adhesion kinase, cotranscriptional activators YAP/TAZ) in mechanosensitive bone cells. Finally, we highlight potential targets for improving mechanoprotection in the treatment of OA. These advances furnish an integration of mechanical regulation of subchondral bone homeostasis, as well as OA therapeutic approaches by modulating mechanical homeostasis.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":"2 2","pages":"Article 100043"},"PeriodicalIF":0.0,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949907024000068/pdfft?md5=2456e3a7b55c2a6f548d64379791d3e8&pid=1-s2.0-S2949907024000068-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139817437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Mechanical impact on biomineralization: Enhancing the strength of composite materials 机械对生物矿化的影响：增强复合材料的强度

Mechanobiology in Medicine Pub Date : 2024-02-02 DOI: 10.1016/j.mbm.2024.100042

Xufeng Niu , Chunyang Ma , Yubo Fan

引用次数: 0

Mechanobiology of Type 1 hypersensitivity: Elucidating the impacts of mechanical forces in allergic reactions 1 型超敏反应的机械生物学：阐明过敏反应中机械力的影响

Mechanobiology in Medicine Pub Date : 2024-02-01 DOI: 10.1016/j.mbm.2024.100041

Henry Sutanto

{"title":"Mechanobiology of Type 1 hypersensitivity: Elucidating the impacts of mechanical forces in allergic reactions","authors":"Henry Sutanto","doi":"10.1016/j.mbm.2024.100041","DOIUrl":"https://doi.org/10.1016/j.mbm.2024.100041","url":null,"abstract":"<div><p>Type 1 hypersensitivity involves an exaggerated immune reaction triggered by allergen exposure, leading to rapid release of inflammatory mediators. Meanwhile, mechanobiology explores how physical forces influence cellular processes, and recent research underscores its relevance in allergic reactions. This review provides a concise overview of Type 1 hypersensitivity, highlighting the pivotal role of mast cells and immunoglobulin E (IgE) antibodies in orchestrating allergic reactions. Recognizing the dynamic nature of cellular responses in allergies, this study subsequently delves into the emerging field of mechanobiology and its significance in understanding the mechanical forces governing immune cell behavior. Furthermore, molecular forces during mast cell activation and degranulation are explored, elucidating the mechanical aspects of IgE binding and cytoskeletal rearrangements. Next, we discuss the intricate interplay between immune cells and the extracellular matrix, emphasizing the impact of matrix stiffness on cellular responses. Additionally, we examine key mechanosensitive signaling pathways, including the mitogen-activated protein kinase (MAPK) pathway, Rho guanosine triphosphatase (GTPase) and integrin-mediated focal adhesion signaling, shedding light on their contributions to hypersensitivity reactions. This interplay of mechanobiology and Type 1 hypersensitivity provides insights into potential therapeutic targets and biomarkers, paving the way for better clinical management of Type 1 hypersensitivity reactions.</p></div>","PeriodicalId":100900,"journal":{"name":"Mechanobiology in Medicine","volume":"2 1","pages":"Article 100041"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2949907024000044/pdfft?md5=adfe1fafbdc05ac9316b2a18d7522f69&pid=1-s2.0-S2949907024000044-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139682632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0