Journal of Natural Pesticide Research最新文献

{"title":"Plant-derivatives for the management of Earias vittella (Fabricius) (Nolidae: Lepidoptera): Current status, challenges, and future prospects","authors":"Satya Singh ,&nbsp;Parthkumar P. Dave ,&nbsp;Neeraj Kumar Sethiya ,&nbsp;Hardev Choudhary","doi":"10.1016/j.napere.2024.100100","DOIUrl":"10.1016/j.napere.2024.100100","url":null,"abstract":"<div><div><em>Earias vittella</em> (Fabricius, 1794) poses a significant threat to Okra production across Asia, Oceania, Africa, Europe, and the Middle East, potentially leading to over 60 % crop losses. Okra, a versatile crop with applications in various industries including pharmaceuticals and food, faces economic peril due to <em>E. vittella</em> infestation, impacting food security. Chemical control methods, while effective, have environmental and health impacts alongside fostering resistance and pest resurgence. Consequently, exploring plant-based alternatives for sustainable pest management has gained traction. This bibliometric study reviewed over 5780 reports published during 1980–2023 and reports 104 research articles selected as per predefined criteria of using plant based <em>E. vittella</em> management in Okra. A total of 108 plant species and their active phytoconstituents belonging to 39 botanical families have been used in different forms comprising of extracts, powders, essential oils, dusts and nanoformulations for <em>E. vittella</em> management in Okra. Their efficacy, impacts on various life parameters of pest, mechanisms of action and their implications in botanical formulation development are also discussed. The review also addresses bibliometric indicators, and the challenges hindering botanical formulations' development faced by various stakeholders. By offering a comprehensive understanding of plant-derived interventions' research landscape, this study aims to address knowledge gaps and provide essential insights for the development of cost-effective, eco-friendly plant-based pest management technologies, crucial for safeguarding Okra production, ultimately contributing to sustainable agriculture.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100100"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142700679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ovicidal, larvicidal and pupicidal activity of the ethyl acetate extracts from soil actinomycetes against tobacco caterpillar, Spodoptera litura (Lepidoptera: Noctuidae)","authors":"M. Kabilan ,&nbsp;Prince Paul ,&nbsp;V. Duraipandiyan ,&nbsp;M. Muthupandi","doi":"10.1016/j.napere.2024.100090","DOIUrl":"10.1016/j.napere.2024.100090","url":null,"abstract":"<div><p>Actinomycetes are known to produce wide-spectrum of secondary metabolites which has a wide variety of biological activities including insecticidal property. <em>Spodoptera litura</em> is a serious pest that damage various commercial cash crops which directly affects the agricultural productivity. The present study is to isolate soil actinomycetes from the terrestrial soil samples collected from the eastern ghats and screened them with various biological screening methods. There were around 100 actinomycetes isolate were obtained using the serial dilution method. It was subjected to antimicrobial screening using streak method (data not shown) and identified active isolates. The most active seven isolates were identified and their secondary metabolites were extracted using ethyl acetate as solvent. All the seven isolates of ethlyacetae extracts were performed the ovicidal, larvicidal and pupicidal bioassays against agricultural insect pest <em>Spodoptera litura</em>. Based on the calculated LC50 values, the most effective crude extract was found to be <em>Streptomyces</em> Sp<strong>.</strong> ERI-TVM-055, ethyl acetate extract. It has exhibited 100 % ovicidal activity at lowest LC50 values of 2.3330 ppm and larvicidal activity was exhibited the 100 % at 25.423 ppm of LC50. It also, exhibited the 100 % pupicidal activity at LC50 of 6.273 ppm<em>.</em> Similarly, <em>Streptomyces</em> sp. ERI-TVM-057, ethyl acetate extract exhibited ovicidal activity against <em>S. litura</em> eggs at LC50 of 11.025 ppm. Over all tested all actinomycetes isolate, the most significant isolate is <em>Streptomyces</em> Sp<strong>.</strong> ERI-TVM-055. The most active extract subjected to GC-MS analysis and the major bio-active molecules were identified. Several compounds present in the crude extract by the study of GC-MS spectrum. The major compounds were identified as Benzene acetic acid (30.19 %) and Acetamide, N-(2- Phenylethyl) (68.37 %) for the insecticidal activity of the active actinomycetes. The present study concluded that <em>Streptomyces</em> Sp<strong>.</strong> ERI-TVM-055 ethyl acetate extract can be used as microbial pesticides as effective one against agricultural pest <em>S. litura</em> and it leads to identify the active molecules and safe the environments.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100090"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000256/pdfft?md5=dc2cb74fc9903bb2304c15a328ab093f&pid=1-s2.0-S2773078624000256-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141839579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating microbial protein elicitors for managing Cabbage Aphids in Brassica oleracea","authors":"Muhammad Salman Hameed ,&nbsp;Abdul Basit ,&nbsp;Muhammad Humza ,&nbsp;Nida Urooj ,&nbsp;Muhammad Qasim ,&nbsp;Muhammad Anwar","doi":"10.1016/j.napere.2024.100098","DOIUrl":"10.1016/j.napere.2024.100098","url":null,"abstract":"<div><div>Cabbage (<em>Brassica oleracea</em>) is a globally important crop but often suffers from damage caused by sucking insect pests and various diseases. This study explores the effectiveness of microbial protein elicitors, such as PeaT1, PeBL1, Hrip1, and PeBA1, in managing Cabbage aphids (<em>Brevicoryne brassicae</em>). It investigates how these elicitors affect phytohormone pathways, including jasmonic acid, salicylic acid, and ethylene, and examines their impact on leaf structures, particularly trichomes. The research offers a novel approach by thoroughly assessing the mechanisms through which microbial protein elicitors control cabbage aphids. It provides valuable insights into potential new strategies for pest management in agriculture. By clarifying how elicitors interact with aphids, this study contributes to developing sustainable pest control and crop protection methods.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100098"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two plant extracts protect stored maize against infestation of Sitophilus zeamais in Northern Ghana","authors":"Abraham Akuoli Issaka Ndebugri ,&nbsp;Joseph Xorxe Kugbe ,&nbsp;Samuel Adu-Acheampong ,&nbsp;Rosina Kyerematen","doi":"10.1016/j.napere.2024.100102","DOIUrl":"10.1016/j.napere.2024.100102","url":null,"abstract":"<div><div>Botanicals have been encouraged as substitutes for conventional pesticides to reduce the impact of the latter on the environment. In line with that, we studied the effects of two plant extracts in controlling <em>Sitophilus zeamais</em> Motsch (Coleoptera: Curculionidae) attacks on stored maize (<em>Zea mays</em> L. Poales: Poaceae). The experiment was conducted under a normal room temperature of 27⁰C to 30⁰C and relative humidity of 74–76 % in a completely randomized experimental design. We infested the stored maize seeds in glass jars with <em>S. zeamais</em> F₁ under a 12-hour day and 12-hour night within 24 hours period. This was after 50 g of the seeds were mixed with 2 g each of the two botanicals separately in different glass jars. There were 5 treatments and 10 replications in total. The data taken for this study were quantitative. To ascertain the efficacy of the botanicals we compared the number of dead and live weevils, of seeds damage and weight loss caused by weevil attacks under each of the different treatments by determining percentage mortality, percent weight loss, seed damage and progeny production. Our results showed that the different botanical formulations significantly controlled <em>S. zeamais</em> population resulting in reduced damage, better protection and improved storage of maize seeds. However, there were no significant differences in protections offered between shade dried neem seed powder and rice husk powder although the latter was the most efficacious with 85 % mortality compared to the former with 65 % with sundried neem seed powder being the least with a little above 40 % mortality. We concluded from this study that the formulated botanicals were very effective in controlling <em>S. zeamais</em> populations and therefore recommended that it be used as a cost effective and environmentally friendly protection of stored maize against <em>S. zeamais</em> attacks, especially in rural poor areas and places known for continuous seed production and storage, such as the case of the study community.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100102"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal activity of citral against Fusarium wilt in tomatoes and induction of the upregulation of glucanase, chitinase, and thaumatin-like protein plant defense genes","authors":"Portia Osei-Obeng ,&nbsp;Leonard Muriithi Kiirika ,&nbsp;Aggrey Bernard Nyende","doi":"10.1016/j.napere.2024.100097","DOIUrl":"10.1016/j.napere.2024.100097","url":null,"abstract":"<div><div><em>Fusarium</em> wilt of tomatoes, caused by the soil-borne fungus <em>Fusarium oxysporum</em> f. <em>sp lycopersici</em> (FOL), is one of the devastating diseases of tomatoes, causing substantial economic losses worldwide. It is primarily controlled with chemical fungicides. The present study assessed the antifungal activity of citral against FOL under greenhouse conditions and investigated its effects on three plant defense genes: glucanase, chitinase, and thaumatin-like protein (TLP) using Real-time- quantitative PCR (RT-qPCR). Greenhouse experiments demonstrated that citral oil at 1024 and 512 µg/mL significantly reduced wilt severity from 61.7 % to 28.3 and 33.3 %, respectively. RT-qPCR results revealed that citral upregulates the expression of the three defense genes to varying extents, highlighting their role in resistance induction in tomatoes against FOL. Chitinase showed the highest differential expression with a 9.54-fold increase at 12 hours post-treatment (hpt), followed by TLP with a 6.96-fold increase at 12hpt, and glucanase with a 3.68-fold increase at 24hpt. These findings suggest citral is a promising biocontrol agent against fusarium wilt in tomatoes. However, field trials are recommended to validate these results under open field conditions.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100097"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142572666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Larvicidal potential of ether extract, and its derivatives fatty acids, and fatty acid methyl esters from Tecoma stans seeds: Bioprospecting for an effective insecticide","authors":"Lucas S. Azevedo ,&nbsp;Thaís P.R. Gonçalves ,&nbsp;Mairon C. Coimbra ,&nbsp;Ezequias P. de Siqueira ,&nbsp;Stênio N. Alves ,&nbsp;Luciana A. R. dos S. Lima","doi":"10.1016/j.napere.2024.100087","DOIUrl":"https://doi.org/10.1016/j.napere.2024.100087","url":null,"abstract":"<div><p>In the work, the larvicidal potential of ether extract (EE), and its hydrolyzed products enriched with fatty acids (FA), and fatty acid methyl esters (FAME) obtained from <em>Tecoma stans</em> seeds was evaluated. EE, and FAME were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS) and three samples were evaluated in a preliminary screen for larvicidal activity against <em>Culex quinquefasciatus</em> at concentrations of 31.25, 62.5, 125, 250, 500, and 1000 μg/mL. The larvicidal efficacy of all samples was assessed at 24, 48, and 144 h post-treatment, detecting the movement of each larva. The data obtained were subjected to probit analysis to determine the lethal concentrations that killed 50 % and 90 % (LC₅₀ and LC₉₀) of the treated larvae. In EE, the phytosterol γ-sitostenone was characterized as the predominant component (30.40 %), while in FAME the presence of methyl palmitate (25.01 %), and methyl stearate (22.14 %) stood out. Notable larvicidal activity was detected after 24 h of treatment. EE exhibited moderate toxicity against <em>C. quinquefasciatus</em> (LC₅₀ = 193.34 μg/mL), while FAME was slightly toxic (LC₅₀ = 269.43 μg/mL), and FA, the most promising product, presented an LC₅₀ = 20.06 μg/mL. The results together demonstrate that extracts, and hydrolyzed products from <em>T. stans</em> seeds may be useful as eco-friendly, economical, and effective larvicidal agents. However, additional studies are needed to search for bioactive compounds of plant extracts, and their derivatives that exhibit larvicidal activity, and their modes of action.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100087"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000220/pdfft?md5=0239c6a255e19843fb4ebeabf1e8d7fe&pid=1-s2.0-S2773078624000220-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141487473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Actinobacteria mediated synthesis of silver nanoparticles using Streptomyces diastaticus and their biological efficacy against human vector mosquitoes and agricultural insect pests","authors":"Manickam Kalpana ,&nbsp;Krishnan Raguvaran ,&nbsp;Thulasiraman Manimegalai ,&nbsp;Suresh Kalaivani ,&nbsp;Palanisamy Devapriya ,&nbsp;Rajan Maheswaran","doi":"10.1016/j.napere.2024.100091","DOIUrl":"10.1016/j.napere.2024.100091","url":null,"abstract":"<div><p>Mosquitoes and agricultural insects were harmful to both public health and crops. Prolonged use of synthetic chemical pesticides for insect pest control was associated with resistance development and negative human and ecological effects. This study investigates the larvicidal activity of Ag-NPs produced using <em>Streptomyces diastaticus</em> against <em>Culex quinquefasciatus</em>, <em>Anopheles stephensi</em>, <em>Aedes aegypti</em>, <em>Spodoptera litura</em>, and <em>Plutella xylostella</em>. UV-Vis spectroscopy, XRD, SEM-EDX, FT-IR, TEM, DLS, and Zeta potential analysis were used to characterize the biosynthesized Ag-NPs. The results showed that biosynthesized Ag-NPs were more toxic to, <em>A. stephensi</em>, <em>C. quinquefasciatus</em>, and <em>A. aegypti</em> with LC₅₀ values of 15.70, 11.10, and 17.53 µg/mL, respectively. Biosynthesized Ag-NPs exhibited the highest antifeedant effects against <em>S. litura</em> (88.73 %) and <em>P. xylostella</em> (92.36 %) at 50 µg/mL concentration. The mortality bioassay revealed considerable larvicidal activity against <em>S. litura</em> and <em>P. xylostella</em> larvae at greater doses of 50 µg/mL, 80.67 and 84.05 %, respectively. Morphogenetic variations were observed on treated larvae of <em>C. quinquefasciatus, A. aegypti, and A. stephensi, S. litura,</em> and <em>P. xylostella</em> with Ag-NPs. In the present investigation the impact of Ag-NPs on the antioxidant enzymes (SOD, CAT, and GPx), the activity of each enzyme were dramatically increased with higher concentrations resulting in significant differences. The study also found that Ag-NPs inhibited the detoxifying enzyme (GST and AChE) activity of target organisms. The histological structure of the treated and untreated larvae revealed structural changes between the midgut epithelial cells. As a consequence of these findings, the <em>S. diastaticus</em>-mediated Ag-NPs serve as a possible, eco-friendly biopesticide and alternative to synthetic chemicals for the control of human vector mosquitoes and destructive arthropod insect pests.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100091"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000268/pdfft?md5=20964b29cb45e07565d3f03d61674643&pid=1-s2.0-S2773078624000268-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141844372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Fungicide from Waste Oil Palm Fruit Bunch for Sustainable Management of Tomato Wilt Disease Caused by Fusarium oxysporum - experimental and in silico studies","authors":"Jacinta Ngozi Akalazu ,&nbsp;Chidi Edbert Duru","doi":"10.1016/j.napere.2024.100095","DOIUrl":"10.1016/j.napere.2024.100095","url":null,"abstract":"<div><p>Tomato wilt, a widespread and persistent disease caused by the fungus <em>Fusarium oxysporum</em>, can be effectively controlled through biological approaches, promoting ecosystem sustainability and enhancing agricultural productivity and quality. This study explored the use of empty oil palm fruit bunch (EOPFB) waste as a natural fungicide to control tomato Fusarium wilt disease through in vitro, pilot scale, and molecular docking experiments. EOPFB aqueous extract showed the highest mycelium growth inhibition of 68.3 %, with the commercial fungicide Mancozeb exhibiting a moderate 42.30 % inhibition, and sterile distilled water having a negligible effect of 0.24 %. The inhibition rate varied with concentration, peaking at 72.63 % at 0.15 g/mL and dropping to 54.80 % at 0.01 g/mL. In terms of wilt disease incidence and severity, EOPFB performed best, recording the lowest incidence of 47.20 % and severity of 15.58 %. Mancozeb followed closely, with an incidence of 46.30 % and severity of 26.23 %. Sterile distilled water, on the other hand, showed the highest incidence of 76.28 % and severity of 56.17 %. Molecular docking simulations corroborated the experimental results, validating the potency of EOPFB as an inhibitor of trypsin from <em>F. oxysporum</em>, and identifying 2-amino-5,6-dimethyl-3 H-pyrimidine-4-one as the primary active compound responsible for the fungicidal activity. This phytochemical, abundant in the crude ethanolic extract of EOPFB, was found to have a higher binding affinity (6.1 kcal/mol) than Mancozeb (4.8 kcal/mol), at the trypsin target. These findings demonstrate the potential of EOPFB, an agricultural waste product, as a sustainable and eco-friendly solution for managing tomato Fusarium wilt disease, contributing to increased tomato production. It also highlights the potential of 2-amino-5,6-dimethyl-3 H-pyrimidine-4-one as a lead compound for the development of novel, natural fungicides, and underscores the value of EOPFB as a rich source of bioactive compounds with agricultural and biotechnological applications.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100095"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S277307862400030X/pdfft?md5=d2fe57967ed5b5f11a68980d743456b7&pid=1-s2.0-S277307862400030X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142271653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effective and eco-friendly botanical insecticidal agents against Spodoptera frugiperda (Noctuidae: Lepidoptera) using the essential oil of Stevia rebaudiana","authors":"Esmat A. El-Solimany ,&nbsp;Antar A. Abdelhamid ,&nbsp;Marwa Adel Thabet ,&nbsp;Mohamed A. Gad","doi":"10.1016/j.napere.2024.100103","DOIUrl":"10.1016/j.napere.2024.100103","url":null,"abstract":"<div><div>Using synthetic pesticides is the main strategy for controlling pests. However, these compounds have caused worry because of their harmful effects on health and their diminishing efficacy against pests that have developed resistance. Consequently, there is a growing interest in adopting more sustainable control methods. <em>Stevia rebaudiana</em> is a valuable medicinal plant used in the food industry for the production of steviol glycosides, a type of natural sweetener. An EO that could be useful for creating innovative insecticides may come from the industrially used plant biomass. In this study, gas chromatography-mass spectrometry (GC-MS) was used to analyse the chemical composition of <em>S. rebaudiana</em> leaves. Sesquiterpenes, or caryophyllene oxide (20.7 %), spathulenol (14.9 %), and (<em>E</em>)-nerolidol (8.0 %), and diterpenes, or phytol (9.2 %), made up the majority of the EO composition. The efficacy of the EO major constituents, namely Phytol, (<em>E</em>)-nerolidol, Spathulenol, and Caryophyllene oxide, was also tested against <em>S. frugiperda.</em> Phytol was the most effective LC₅₀ = 14.38 mg/L, followed by (<em>E</em>)-nerolidol LC₅₀ = 15.88 mg/L, Spathulenol LC₅₀ = 18.42 mg/L, and Caryophyllene oxide LC₅₀ = 23.41 mg/L. Furthermore, some of the biological and histological features of the extracts were also studied in a lab setting. Overall, <em>Stevia rebaudiana</em> (Bertoni) should be given more consideration in the development of environmentally safe and efficient pesticides.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100103"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A botanical nanoemulsion against phytopathogenic fungi Colletotrichum sp. and Fusarium oxysporum: Preparation, in vitro and in vivo bioassay","authors":"Quang Le Dang ,&nbsp;Cuong Quoc Nguyen ,&nbsp;Thi Kieu Anh Vo ,&nbsp;Thi Thu Trang Nguyen ,&nbsp;Quang Duong Pham ,&nbsp;Thi Xuyen Nguyen ,&nbsp;Thi Hong Cao ,&nbsp;Quang De Tran ,&nbsp;The Tam Le ,&nbsp;Thi Hanh Do ,&nbsp;Van Tan Chu ,&nbsp;Thi Bich Hong Nguyen","doi":"10.1016/j.napere.2024.100099","DOIUrl":"10.1016/j.napere.2024.100099","url":null,"abstract":"<div><div>A botanical nanoemulsion (NE) comprised of cinnamaldehyde (from Cinnamomum cassia oil) and annonaceous acetogenin (AAs) (from Annona squamocin seeds extract) was fabricated using sonication. The nanoemulsion was tested for their in vitro and in vivo antifungal activity against Colletotrichum sp. and Fusarium oxysporum the disease-causal agents in fruit and vegetable crops. The best average of nanoemulsion hydrodynamic diameter was 245.3 nm, and the polydispersity index (PI) was 0.499 based on DLS measurement. Zeta potential of the nanoemulsion was determined to be −44.2 mV, indicating the stability of the droplets. In vitro antifungal bioassay of the nanoemulsion showed the best inhibition against Colletotrichum sp. by 53.9 % when tested at a dilution rate of 1:100 (v/v). For F. oxysporum, the nanomaterial also inhibited mycelial growth by 91.6 % at the same dilution rate. When tested at dilution rates of 1:200 and 1:400 (v/v), the nanoemulsions caused moderate and weak inhibitions from 30.9 % to 52.8 % for all fungi. In vivo experiment, the nano-emulsion at 1:200 (v/v) dilution demonstrated an antifungal efficacy of 57.1 % (after 4 days) and 49.6 % (after 7 days) of testing. Besides, the mechanism of action has also been elucidated through molecular interaction models between the main compounds with eburicol 14α-demethylase. Our research indicates that botanical nanoemulsion containing cinnamon oil and AAs have a remarkable potential to suppress phytopathogenic fungi and prevent the spread of fungal diseases in tropical fruits and vegetables such as tomato and other crops of Solanaceae family.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100099"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142700678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}