Toxic and synergetic effect of plant essential oils along with nano-emulsion for control of three mosquito species

Journal of Natural Pesticide Research Pub Date : 2023-09-01 DOI:10.1016/j.napere.2023.100045

Perumal Vivekanandhan , Sukanya Panikar , Veeran Sethuraman , Ayyakkannu Usha-Raja-Nanthini , Muthugounder Subramanian Shivakumar

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引用次数: 4

Abstract

Nano-emulsion containing Eucalyptus grandis (W. Hill ex Maiden) and Corymbia citrodora (Hook.) K.D.Hill & L.A.S.Johnson (common synonym Eucalyptus citriodora Hook.) leaf essential oils individual, combination was evaluated its larvicidal activity against Anopheles stephensi, Aedesaegypti and Culex quinquefasciatus mosquito larvae at 24 h post treatment. Nano-emulsion had low polydispersivity index and mean droplet size was below 66 nm. After 24 h E. grandis essential oil exhibited significant larvicidal activity with LC₅₀ and LC₉₀ values in A. aegypti (23.667; 438.797 ppm in A. aegypti A. stephensi (10.984; 277.535 ppm) and C. quinquefasciatus (4.676; 106.415 ppm). Similarly in C. citrodora showed low LC₅₀ and LC₉₀ values in A. aegypti (18.430; 1048.599 ppm), A. stephensi (6.573; 176.737 ppm) and C. quinquefasciatus (7.917; 287.720 ppm) respectively. The combination of E. grandis and C. citrodora essential oils showed higher mortality rates than individual essential oils, with low LC₅₀ and LC₉₀ values in A. aegypti (3.625; 58.962 ppm), A. stephensi (3.245; 34.473 ppm) and C. quinquefasciatus (3.002; 30.153 ppm). Nano-emulsion exhibited toxic effects similar to combination group with LC₅₀ and LC₉₀ values in A. aegypti (5.344; 113.362 ppm), A. stephensi (3.183; 83.112 ppm) and C. quinquefasciatus (2.832; 166.665 ppm). Chemical constituents of both essential oil were analyzed using GC-MS and FT-IR, result showed E. grandis contain different compounds namely; alpha-pinene, (31.74%); o-cymene, (17.84%); Eucalyptol (16.24%); Bicyclo[2.2.1]heptane (3.2%) and C. citrodora showed Citronellol (49.31%); dl-Isopulegol (25.39%) as a major compound while their synergistic action may be due to type’s composition of EOs might produce the synergist effect along with nanoemulsions. Our findings suggest that combination of essential oil E. grandis and C. citrodora can be used for mosquito control as green approach.

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植物精油与纳米乳剂对三种蚊的毒性及协同效应研究

含有巨桉（W.Hill ex Maiden）和香茅珊瑚（Hook.）K.D.Hill的纳米乳液；L.A.S.Johnson（共有异名柠檬桉）叶精油个体、组合在处理后24小时对斯氏按蚊、埃及伊蚊和致倦库蚊幼虫的杀幼虫活性进行了评估。纳米乳液具有低的多分散性指数，平均液滴尺寸小于66nm。24小时后，E.grandis精油表现出显著的杀幼虫活性，埃及伊蚊的LC50和LC90值为23.667；斯蒂芬伊蚊的438.797ppm（10.984；277.535ppm）和致倦库蚊的4.676；106.415ppm，斯氏A.stephensi（6.573；176.737ppm）和致倦库蚊（7.917；287.720ppm）。香茅精油和香茅精油组合的死亡率高于单独精油，埃及伊蚊（3.625；58.962 ppm）、斯氏伊蚊（3.245；34.473 ppm）和致倦库蚊（3.002；30.153 ppm）的LC50和LC90值较低，用GC-MS和FT-IR对两种挥发油的化学成分进行了分析，结果表明，大白菜中含有不同的化合物，即：；α-蒎烯（31.74%）；o-cymene（17.84%）；桉树醇（16.24%）；双环[2.2.1]庚烷（3.2%）和C.citodora显示香茅醇（49.31%）；dl异蒲列醇（25.39%）作为主要化合物，而它们的协同作用可能是由于EOs的类型组成，可能与纳米乳液一起产生协同作用。我们的研究结果表明，香茅和香茅精油的组合可以作为绿色方法用于控制蚊子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Natural Pesticide Research

CiteScore

2.30

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0.00%

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