Journal of Natural Pesticide Research最新文献

{"title":"Sustainable pest management with moroccan essential oils: Insights into insecticidal, behavioral effects, and factors influencing bioactivity","authors":"Houssam Annaz ,&nbsp;Noureddin Bouayad ,&nbsp;Ayoub Kounnoun ,&nbsp;Francesco Cacciola ,&nbsp;Kacem Rharrabe","doi":"10.1016/j.napere.2025.100137","DOIUrl":"10.1016/j.napere.2025.100137","url":null,"abstract":"<div><div>Morocco is widely known for its biodiversity in flora, notably medicinal and aromatic plants (MAP). These plants are widely distributed across the country in different climates, latitudes, and altitudes, and have been reported for various biological activities. This review aims to showcase the insecticidal and behavioral effects of Moroccan essential oils (EO) extracted from different plant families against insect pests. All eligible studies were retrieved from Scopus and Web of Science following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines using ‘Essential oil” and “Insect*” as keywords where 11,400 articles were found and only 58 studies met the aim of this review. The analysis of articles retrieved from Scopus and Web of Science revealed a strong dominance of EOs extracted from the Lamiaceae family, where <em>Mentha pulegium</em> and <em>Rosmarinus officinalis</em> appear as the most studied species. In terms of Insects, the Coleoptera order was the most species evaluated for different bioassays, where <em>Callosobruchus maculatus</em> and <em>Tribolium castaneum</em> were the most studied species. In terms of bioactivities, Moroccan EOs exhibited a potent insecticidal activity via fumigation, contact, ingestion, and topical application. These EOs also affected the behavior of insects, leading to repellent, feeding deterrent, and anti-settling effects. Besides, this review highlights the impact of several factors on reported bioactivities. These factors can be related to EOs such as harvest period, geographical distribution, plant part used, and others related to the insects, such as insect species, developmental stages, and sex of adults. In brief, the diversity of EOs extracted from Moroccan Plants shows a promising capacity for the development of sustainable, natural, and harmless solutions for the control of pests.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"13 ","pages":"Article 100137"},"PeriodicalIF":0.0,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144194652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of nanoemulsions from Oocotea odorifera (Vell.) rohwer through a low energy approach: An ecological larvicide against Aedes aegypti and Culex quinquefasciatus","authors":"Caio P. Fernandes ,&nbsp;Jonatas L. Duarte ,&nbsp;Desirane C. Bezerra ,&nbsp;Anna E.M.F.M. Oliveira ,&nbsp;Rodrigo A.S. Cruz ,&nbsp;Lisset Ortiz-Zamora ,&nbsp;Wanderlei do Amaral ,&nbsp;Cícero Deschamps ,&nbsp;Alessandra L. Valverde ,&nbsp;Lorane I.S. Hage-Melim ,&nbsp;Ricardo M.A. Ferreira ,&nbsp;Mateus A. Batista ,&nbsp;Raimundo N.P. Souto ,&nbsp;Luiz E. da Silva","doi":"10.1016/j.napere.2025.100136","DOIUrl":"10.1016/j.napere.2025.100136","url":null,"abstract":"<div><div><em>Ocotea odorifera</em> is also known as sassafras and cinnamon sassafras. It was used as flavoring agent in beverages and other food industrial products, being now used in disinfectants and insecticides. The essential oil, of sassafras, as well as other Brazilian species, has great potential for the food industry, although it is occasionally affected by its volatile composition. New products, such as aqueous nano-emulsions, are considered extremely powerful since they enhance the properties of the oils. Therefore, the aim of this study was to develop this type of colloidal systems with <em>O. odorifera</em> essential oil. The best results were achieved with blends of polysorbate 20/sorbitan monooleate (size diameter &lt;200 nm and polydispersity index &lt;0.1) and no major alterations were observed after the storage. The critical interpretation of the molecular characteristics of the phytochemicals of this essential oil presents an interesting analysis about these essential oil-based nano-emulsions. For <em>Aedes aegypti</em> the estimated LC₅₀ and LC₉₀ values, are respectively, 37.646 and 46.3408 ppm; while for <em>Culex quinquefasciatus</em> the estimated LC₅₀ and LC₉₀ values are 33.3365 and 47.8939 ppm, respectively. The low energy/ecofriendly approach using different non-ionic surfactants by a non-heating method is in accordance with a sustainable concept. Therefore, it opens perspectives for the preparation of sassafras nano-emulsions.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"13 ","pages":"Article 100136"},"PeriodicalIF":0.0,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144221817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Moroccan brown algae as a source of bionematicidal against root-knot nematode (Meloidogyne spp.)","authors":"Mouna Belmouden ,&nbsp;Khadija Bouftini ,&nbsp;Amina Braimi ,&nbsp;Bouchra El Aissaoui ,&nbsp;Mammass Bourassen ,&nbsp;Ibtissam Sabir ,&nbsp;Ilyass Filali Alaoui ,&nbsp;Tayeb Obidari ,&nbsp;Rachid Nmila ,&nbsp;El Hassan Mayad ,&nbsp;Halima Rchid","doi":"10.1016/j.napere.2025.100135","DOIUrl":"10.1016/j.napere.2025.100135","url":null,"abstract":"<div><div>Root-knot nematodes (<em>Meloidogyne</em> spp.) pose a major threat to vegetable production in Morocco. In search of sustainable alternatives to synthetic nematicides, this study evaluated the <em>in vitro</em> nematicidal activity of ethanolic extracts from four Moroccan brown algae: <em>Cystoseira humilis</em>, <em>Cystoseira ericoides</em>, <em>Sargassum vulgare</em>, and <em>Sargassum muticum</em> at concentrations of 6, 8, and 10 mg/mL. The extracts of <em>S. vulgare</em> and <em>C. humilis</em> achieved the highest juvenile mortality (83 %) after 72 hours. <em>S. vulgare</em> also showed the greatest inhibition of egg hatching (82 %). Phytochemical analysis revealed that <em>C. humilis</em> and <em>C. ericoides</em> had the highest levels of total phenolics and flavonoids, while <em>S. vulgare</em> demonstrated strong nematicidal activity despite lower compound concentrations. These findings highlight the potential of <em>S. vulgare</em> and <em>Cystoseira</em> species as eco-friendly agents for managing <em>Meloidogyne spp.</em> Further <em>in vivo</em> studies are required to confirm their efficacy, determine optimal application methods, and establish effective dosages.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"13 ","pages":"Article 100135"},"PeriodicalIF":0.0,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144135133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological control of Alternaria alternata MW970059, the causal agent of tomato rot, by Aspergillus nidulans MW732187 isolated from green household waste","authors":"Fatima Zahrae Moussaid ,&nbsp;Rachid Lahlali ,&nbsp;Said Ezrari ,&nbsp;Azeddin El Barnossi ,&nbsp;Abdelilah Iraqi Housseini","doi":"10.1016/j.napere.2025.100134","DOIUrl":"10.1016/j.napere.2025.100134","url":null,"abstract":"<div><div>The development of fungal resistance to fungicides is a significant challenge in agricultural disease management, and the research axis is in current scientific discourse. The objective of the current study aims to search for new biological agents to control <em>Alternaria alternata</em> (<em>A. alternata</em>) causal agent of tomato rot. 73 fungal isolates where tested in the <em>in vitro</em> screening. <em>Aspergillus</em> sp. isolated from decayed tangerine peel microflora, showed the performing antagonistic strain against <em>A. alternata</em>. <em>Aspergillus</em> sp. exhibited significant inhibition of <em>A. alternata</em>; the growth fungal inhibition (%FI) rate was 66.66 ± 0.25 % causing various morphological mycelia changes, swellings, deformations and vacuolization. <em>A. alternata</em> and the antagonist <em>Aspergillus</em> sp. were identified based on morphology trails and molecular tool as <em>A. alternata</em> and <em>Aspergillus nidulans</em>, respectively. Cell-free supernatant (CFS) showed that the metabolites produced by <em>A. nidulans</em> are involved in the control of <em>A. alternata</em>. The half maximal effective concentration (EC₅₀) value of <em>A. alternata</em> was estimated at 1 % (v/v), the inhibition rate was 50.97 ± 0.85 %. Fourier Transform Infra-Red (FT-IR Spectrometry) analysis of organic (ethanol, methanol) and aqueous extracts of CFS revealed distinct characteristic peak values corresponding to various suggested functional groups in the extracts such as phenolic, lipids, and possibly proteins/peptides compounds. <em>In vivo</em> application of <em>A. nidulans</em> strain to tomato fruits significantly reduced postharvest decay caused by <em>A. alternata</em> by 41.34 % after the 10^th day of inoculation. Overall, the findings from this study suggest that <em>A. nidulans</em> strain may be a potential promising bio-agent to control the phytopathogen <em>A. alternata</em>.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"13 ","pages":"Article 100134"},"PeriodicalIF":0.0,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144116813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing indigenous entomopathogenic fungi for biopesticide development against Tuta absoluta in Tanzania","authors":"Camila Renson ,&nbsp;Modester D. Nkungu ,&nbsp;Regina P. Mtei","doi":"10.1016/j.napere.2025.100133","DOIUrl":"10.1016/j.napere.2025.100133","url":null,"abstract":"<div><div>The invasive pest <em>Tuta absoluta</em> (Meyrick) poses a significant risk to global tomato crops, particularly affecting Tanzania's food security and economy. Currently, control strategies for this pest rely on synthetic pesticides, which are ineffective and pose a great environmental threat to the ecosystem. This underscores the urgent need for search of ecofriendly alternative to combat this invasive pest. The agar plate method was employed for isolation and purification of fungi from the soil. From soil samples collected in Arusha's tomato fields, 36 fungal isolates were discovered, and subjected to rigorous morphological and biochemical characterization. Of these, 8 demonstrating enzyme activity capable of breaking down insect cuticles, indicating their potential as insecticides. These isolates were subjected molecular characterizations, including DNA sequencing and analyses using the BLAST program available at the National Center for Biological Information (NCBI), to ascertain their identity. The isolates showed 100 % similarity with <em>Aspergillus terreus</em>, <em>Penicillium steckii</em>, <em>Purpureocillium lilacinum, Aspergillus flavus and Aspergillus versicolor</em> of GeneBank accession numbers MN463005.1, MK179265.1, MN242828.1, MN559660.1 and MH270605.1, respectively. Bioassays conducted on <em>T. absoluta</em> larvae with spore suspensions of these fungi revealed significant larval mortality rates for <em>A. terreus</em>, <em>P. steckii</em>, and two strains of <em>P. lilacinum</em>, with <em>Penicillium steckii</em> achieving up to 100 % mortality at a concentration of 10⁷ spores/mL. The study findings highlight the potential of using native fungi, particularly <em>Aspergillus terreus</em>, <em>Penicillium steckii</em>, and <em>Purpureocillium lilacinum</em>, as effective biopesticides against <em>T. absoluta</em>, underscoring the importance of harnessing indigenous microbial resources for sustainable agricultural pest control and food security.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"13 ","pages":"Article 100133"},"PeriodicalIF":0.0,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144105288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial control of forest insect pests over 60 years (1964–2024): Network analysis and bibliometric mapping","authors":"Deepak Kumar Mahanta ,&nbsp;Charishma Krishnappa ,&nbsp;Tanmaya Kumar Bhoi","doi":"10.1016/j.napere.2025.100132","DOIUrl":"10.1016/j.napere.2025.100132","url":null,"abstract":"<div><div>Microbial control of forest insects has emerged as a viable alternative to conventional chemical pesticides, providing sustainable strategies for managing forest pest populations. This bibliometric analysis examines the progression of research in this domain from 1964 to 2024, emphasizing the application of entomopathogenic fungi, bacteria, viruses, and nematodes as biological control agents. VOSviewer, Bibliometrix, and Biblioshiny from R were employed for statistical analysis of the articles retrieved from the Scopus database. The research indicates a substantial rise in articles concerning microbial control during the early 2000s, underscoring an increasing awareness of its potential in forest management. Prominent biocontrol agents, including <em>Beauveria bassiana</em>, <em>Metarhizium anisopliae</em>, and <em>Bacillus thuringiensis</em>, have been recognized for their efficacy in managing diverse forest pests, such as the gypsy moth (<em>Lymantria dispar</em>), bark beetles, and defoliators. The research highlights the increasing significance of microbial agents in minimizing pest outbreaks intensified by climate change, invading species, and the constraints of chemical pesticides. Notwithstanding the optimistic outlook, the study also delineates additional challenges, such as ecological hazards, variable efficacy across different climatic conditions, regulatory obstacles, and the absence of long-term field data. These problems underscore the necessity for more studies to enhance the use of microbial management in forest ecosystems, ensuring its efficacy and safety. This research offers a thorough examination of the present condition of microbial control in forest pest management, highlighting the necessity of creating dependable, environmentally sustainable alternatives to maintain forest health and biodiversity amid increasing environmental challenges.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"12 ","pages":"Article 100132"},"PeriodicalIF":0.0,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antagonistic effect of 7-hydroxytropolone and its substitutes against Dickeya solani","authors":"Euphrasie Munier-Lépinay ,&nbsp;Christine Cézard ,&nbsp;Mounia Khelifa ,&nbsp;Serge Pilard ,&nbsp;Sylvain Laclef","doi":"10.1016/j.napere.2025.100131","DOIUrl":"10.1016/j.napere.2025.100131","url":null,"abstract":"<div><div><em>Dickeya</em> species are major plant pathogenic bacteria worldwide, with <em>Dickeya solani</em> (<em>D. solani</em>) posing a key threat to potato crops, which rank fourth among the most cultivated food crop globally. <em>D. solani</em> is responsible for blackleg disease, causing significant yield and quality losses in seed potato production. Current control options, whether phytochemicals or biocontrol strategies against blackleg disease, remain limited. However, the potato rhizosphere bacterium <em>Pseudomonas</em> PA14H7 has shown effective antagonism against <em>D. solani via</em> the production of 7-hydroxytropolone (7-HT). This molecule was identified in the filtered supernatant of <em>Pseudomonas</em> PA14H7 (FS-PA14H7) complexed with iron. Herein, we demonstrate that the activity of 7-HT is strongly linked to its ability to chelate iron, thereby inhibiting the growth of <em>D. solani</em> through iron deprivation. Given the challenges associated with synthesizing 7-HT, alternative commercially available molecules with the same elemental composition and bearing functional groups such as catechol, phenol or carboxylic acid were screened for their ability to chelate iron using computational calculations and mass spectrometry techniques (UPLC-HRMS and MS/MS). Then, their antibacterial activity against <em>D. solani</em> and other Soft Rot <em>Pectobacteriaceae</em> was evaluated and compared with the one of 7-HT. Three candidates 2,3, 2,4 and 2,5- dihydroxybenzaldehydes were identified as potential substitutes, presenting promising biocontrol solutions for managing potato blackleg disease.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"13 ","pages":"Article 100131"},"PeriodicalIF":0.0,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143911747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-sustainable agriculture practices through Mycoviruses","authors":"Kishalay Paria ,&nbsp;Smritikana Pyne ,&nbsp;Rupak Khamarai ,&nbsp;Susanta Kumar Chakraborty","doi":"10.1016/j.napere.2025.100129","DOIUrl":"10.1016/j.napere.2025.100129","url":null,"abstract":"<div><div>Modern agricultural practices for the production of food grains are seen as a boon to human civilization, as they meet the needs of people for staple foods, organic matter, and essential micronutrients. However, the use of agrochemicals poses a serious pollution threat to soil and aquatic ecosystems. The present study attempts to develop ways and means for effective management of pollution caused by agrochemicals through the application of mycoviruses, which makes a significant contribution to the transition economy involving various socio-economic and environmental parameters. The present paper also has reviewed the recent literature related to various studies pertaining to developing more tolerant crops against eco-biological stress with the involvement of new innovative microbial biotechnological methods based on the operating principles of molecular biology. The infection efficiency of mycovirus is influenced by the biological characteristics of both the virus and the host plant. In the emerging ecological and economical adverse conditions, scopes of applicability of mycoviruses have opened up new perspectives on the possibility of developing a mycoviral-based novel bio-control system (such as RNA silencing, autophagy and plant incompatibility) for large-scale effective eco-sustainable and low-cost agricultural operations, with an aim at maintaining the ecologically healthy state of agricultural fields.This paper also focuses on the multidimensional environmental benefits to the agricultural sectors depending on the potential of standardizing this eco-friendly approach in order to solve the problem of pest infestations and thereby to reduce large scale application of chemical herbicide and pesticide in cost effective way.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"13 ","pages":"Article 100129"},"PeriodicalIF":0.0,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Image-based blight disease detection in crops using ensemble deep neural networks for agricultural applications","authors":"Md Mohinur Rahaman ,&nbsp;Saiyed Umer ,&nbsp;Md Azharuddin ,&nbsp;Asmaul Hassan","doi":"10.1016/j.napere.2025.100130","DOIUrl":"10.1016/j.napere.2025.100130","url":null,"abstract":"<div><div>Blight disease poses a significant threat to agricultural output that results in large crop losses worldwide. Plant diseases must be promptly identified and managed to maintain crop health and maximise yields. This research presents a novel ensemble-based deep-learning model for plant blight disease detection, especially for agricultural applications. The suggested model uses convolutional neural networks (CNNs) for image recognition to accurately and automatically detect blight-affected areas in plant leaf images. An extensive dataset of plant leaf images was gathered to train and evaluate the model, including samples from both healthy and diseased plants. This ensemble-based deep learning model outperformed conventional deep learning and machine learning models in extracting characteristics that differentiated between plants affected by blight and those that weren’t. The proposed model (ResNet11) is a dependable and effective tool for on-the-spot disease detection in the field of potato, tomato and pepper, as demonstrated by experimental results that illustrate an accuracy of over 99 % for potato and pepper crops as a 3-class and 2-class problem respectively. Moreover, we get an accuracy of over 87 % for tomato plants as a 10-class problem.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"12 ","pages":"Article 100130"},"PeriodicalIF":0.0,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nematicidal efficacy of native Trichoderma isolates on Meloidogyne incognita and their influence on tomato growth parameters","authors":"Puja Jaiswal ,&nbsp;Ram Bahadur Khadka ,&nbsp;Suraj Baidya ,&nbsp;Aashaq Hussain Bhat ,&nbsp;Arvind Kumar Keshari","doi":"10.1016/j.napere.2025.100128","DOIUrl":"10.1016/j.napere.2025.100128","url":null,"abstract":"<div><div>Root-knot nematodes (RKN), particularly <em>Meloidogyne incognita</em>, pose a major threat to global agriculture, causing significant crop losses. While fungal antagonists like <em>Trichoderma</em> spp. offer eco-friendly alternatives to chemical nematicides, limited research exists on the comparative efficacy of native <em>Trichoderma</em> spp. in controlling <em>M. incognita</em> and enhancing plant growth under both controlled and greenhouse conditions. This study aims to bridge this gap by identifying the most effective <em>Trichoderma</em> spp. with strong nematicidal potential. The primary objectives of this study were to evaluate the nematicidal activity of 167 <em>Trichoderma</em> isolates against <em>M. incognita</em> second-stage juveniles (J2) under in vitro conditions, assess the most potent species in greenhouse conditions for their ability to suppress root galling and nematode population, and determine the impact of these species on tomato plant growth parameters. A total of 167 <em>Trichoderma</em> isolates were screened in vitro for their ability to induce mortality in <em>M. incognita</em> second-stage juveniles (J2) at 24, 48, and 72 h. The most effective species were further tested in greenhouse conditions using <em>Trichoderma</em> conidial suspensions (10⁶ spores/mL) to assess their impact on root galling, nematode population density, and tomato growth. Among them, 22 isolates exhibited strong nematicidal activity (70 %) within 72 h, with <em>T. asperellum</em> SolT6b achieving the highest J2 mortality (91 %), followed by <em>T. afroharzianum</em> ST10 (88.63 %). In greenhouse conditions, SolT6b and ST10 significantly reduced root galling (by 90.4 % and 89.3 %, respectively) and J2 soil populations (by 67.7 % and 66.6 %, respectively) compared to untreated controls. Additionally, <em>Trichoderma</em> treatment enhanced root biomass and overall plant vigor. These findings highlight the potential of native <em>Trichoderma</em> spp. as sustainable biocontrol agents against <em>M. incognita</em>, offering an effective alternative to chemical nematicides and paving the way for <em>Trichoderma</em>-based biocontrol formulations.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"12 ","pages":"Article 100128"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143761200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}