Journal of Natural Pesticide Research最新文献

{"title":"Novel Rhizophus oryzae esterase-immobilized chitosan/carbon nanomaterials as sensing support for enzyme inhibition-based biosensor in the detection of organophosphorus pesticide residues on crops","authors":"M.F. Amir Hafiz ,&nbsp;Farah B. Ahmad ,&nbsp;M.H. Maziati Akmal","doi":"10.1016/j.napere.2024.100092","DOIUrl":"10.1016/j.napere.2024.100092","url":null,"abstract":"<div><p>The use of mobile biosensor would allow for cost-effective and rapid on-site detection of organophosphorus (OP) pesticide. Immobilizing enzyme on electrode would improve the sensitivity of biosensor due to the nature of its specific active site-binding. OP inhibition on esterase enzyme is used as the sensing mechanism of the biosensor. In this study, microbial esterase from <em>Rhizopus oryzae</em> (RoE) was immobilized into modified screen-printed carbon electrode (SPCE) for the fabrication of novel RoE/chitosan/nanomaterial-COOH/SPCE biosensor for the detection of methyl parathion, an OP pesticide. The first phase of this study aims to analyze biochemical characteristics of 1-naphthyl acetate (1-NA) hydrolysis by RoE and enzymatic inhibition of RoE by OP pesticide. RoE kinetic showed the best fit with Langmuir model with the highest linear regression (<em>R</em>² = 0.996). The inhibition of RoE by OP was shown to be linearly proportional to the enzyme activity (<em>R</em>² = 0.827). The second phase of this study aims to analyze electrochemical performance of RoE-immobilized biosensor. Various carbon-based sensing support nanomaterials (graphene nanoplatelets (GNP), graphite and multi-walled carbon nanotube (MWCNT)) was functionalised by carboxyl (-COOH) group and screened, where all nanomaterials showed effective sensitivity towards detecting 0.01 ng/L methyl parathion pesticide. GNP-based biosensor exhibited the highest degree of inhibition (9.94 %) and better reproducibility after the first cycle. RoE/chitosan/GNP-COOH/SPCE biosensor is promising to be developed as cost-effective and highly sensitive biosensor for OP pesticide detection.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100092"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S277307862400027X/pdfft?md5=7c76a734a63fefa2bdce3b6b24901a8b&pid=1-s2.0-S277307862400027X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141839698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemicals, UPLC-QTOF-MS/MS analysis, potential acaricidal activity and molecular modeling of Datura metel grown in North-Eastern India","authors":"Aditi Kundu ,&nbsp;Karivaradharajan Swarnalakshmi ,&nbsp;Sushmita Rajkhowa ,&nbsp;Anirban Barik ,&nbsp;S.N. Bhagyasree ,&nbsp;Tankeswar Nath ,&nbsp;Bidyut Kumar Sarmah ,&nbsp;Srikant Ghosh","doi":"10.1016/j.napere.2024.100086","DOIUrl":"https://doi.org/10.1016/j.napere.2024.100086","url":null,"abstract":"<div><p>The present investigation emphasized exploitation of <em>Datura metel</em> L. for potential acaricidal activity. Extraction method suggested higher extraction efficiency with the use of 50 % methanol over aqueous medium. UPLC-QTOF-ESI-MS analysis of the extracts resulted tentative identification of eighteen tropane alkaloids such as tigloidin, hyoscyamine N-oxide, scopolamine N-oxide, hyocyamine, hydroxyapoatropine, tropinone, scopine, hyoscine, atropine, valtropine, ditigloyloxytropane, ditigloyloxytropanol, apohyoscine, tigloyloxytropane, norhyoscine, meteloidine, cuscohygrine and tropine in the aerial parts (EAP) and roots (ER). Further, nine phenolic acids such as gallic, protocatechuic, chlorogenic, vanillic, p-coumaric, ferulic, quinic, syringic, and sinapic acids along with fourteen flavonoids such as luteolin-7-glucoside, catechin-3-O-rhamnoside, kempferol-3,7-O-diglucoside, naringenin-6-β-D-glucopyranoside, quercetin 3-O-glucosyl-xyloside, apigenin-7-O-glucoside, flavonol-3-glucoside, luteolin, epicatechin, chrysoeriol, diadzein, genistein, hesperidin and hydroxycoumarin were also identified. Highest acaricidal activity was observed for ER showing LC₅₀ and LC₉₀ of 112.5 and 317.3 μg/mL after 48 h of exposure, respectively. Molecular modeling analysis revealed favourable interaction of tigloidine with UDP transferase of <em>T. urticae</em> through attractive charge, pi-alkyl, alkyl and conventional H bonds. Though, ER was found to be phytotoxic with the highest root growth inhibition in terms of IC₅₀ value of 131.2 and 184.9 μg/mL against <em>Chenopodium album</em> and <em>Triticum aestivum</em>, respectively. Being eco-benign natural products, <em>Datura</em> phytochemicals can serve as potential bio-acaricide, thereby, a smart delivery system could be developed comprising synergistic concoction of the alkaloid rich phytochemical fractions for efficient management of the notorious acarids.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100086"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000219/pdfft?md5=c9ca56be676733a1d772d86cfeba1af7&pid=1-s2.0-S2773078624000219-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141434959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibitory effects of wild Origanum elongatum extracts on Fusarium oxysporum mycelium growth and spores germination: Evidence from in-vitro, in-planta, and in-silico experiments","authors":"Amal Hari ,&nbsp;Rachid Lahlali ,&nbsp;Ahmet Buğra Ortaakarsu ,&nbsp;Noamane Taarji ,&nbsp;Salah-Eddine Laasli ,&nbsp;Khawla Karaaoui ,&nbsp;Meryem Benjelloun ,&nbsp;Wafae Ouchari ,&nbsp;Mansour Sobeh ,&nbsp;Ghizlane Echchgadda","doi":"10.1016/j.napere.2024.100096","DOIUrl":"10.1016/j.napere.2024.100096","url":null,"abstract":"<div><div><em>Fusarium</em> wilt, caused by <em>Fusarium oxysporum,</em> is one of the most devastating diseases to crops including potatoes. This fungus is widespread and dispersed via resistant spores. In this study, organic (methanol, hexane, and chloroform) and aqueous extracts of the aerial parts of wild <em>Origanum elongatum</em> were tested against mycelium and spores of <em>F. oxysporum</em> in both <em>in-vitro</em> (using the poisoned food technique) and <em>in-planta</em> assays. Further, the chemical composition of the extract was identified <em>via</em> HPLC-PDA-MS/MS. Then, molecular modeling was used to clarify the antifungal activity of identified biomolecules. The obtained results showed that organic and aqueous extracts showed significant antifungal and anti-sporulation activities with different effectiveness depending on the extract, concentration, and application test. In <em>in-vitro</em> assays of the aqueous extract, the highest antifungal activity was recorded at concentrations of 10 mg mL⁻¹, while the highest anti-sporulation activity (91.79 %) was observed at 50 mg mL⁻¹. In the organic extracts, methanol was the most effective against fungus with 100 % inhibition of mycelium at 5 mg mL ⁻¹ and the highest anti-sporulation effect (73.58 %) at 20 mg mL⁻¹. In <em>in-planta</em> assays, the methanolic extract at 20 mg mL⁻¹ achieved 0 % disease severity, while the aqueous extract at 50 mg mL⁻¹ reduced severity to 7 %, both significantly more effective than the untreated control. Therefore, methanol was the most effective and these biological properties are supported by a wide range of bioactive molecules, including 56 molecules compared to only 28 molecules in the aqueous extract. These include glycosides of apigenin and kaempferol and salvianolic acid b. However, more research is needed to clarify the inhibition action of the individual components and their synergetic actions in controlled and field conditions.</div></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100096"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring chemical composition and insecticidal activities of Alpinia calcarata rhizome essential oil against three major storage insects","authors":"Pathikalayil R. Anuranj,&nbsp;Naduvilthara U. Visakh,&nbsp;Berin Pathrose,&nbsp;Shiela Betsy George","doi":"10.1016/j.napere.2024.100088","DOIUrl":"10.1016/j.napere.2024.100088","url":null,"abstract":"<div><p><em>Alpinia calcarata</em> (Roscoe), generally recognized as snap ginger or lesser galangal of the Zingiberaceae family, undergoes widespread cultivation in tropical nations. It is characterized by its noteworthy pharmacological attributes, such as anti-inflammatory, analgesic, antinociceptive, reproductive, anti-cancer, and gastro-protective effects. Many storage pests now resist traditional chemical pesticides. Transitioning to organic alternatives is a preferable and sustainable solution. This research investigates the insecticidal activity of essential oil from <em>A. calcarata</em> rhizomes, obtained via hydro-distillation. Chemical analysis was conducted, and the study assessed its toxicity and repellent effects on <em>Tribolium castaneum</em> (Herbst), <em>Lasioderma serricorne</em> (F.), and <em>Callosobruchus chinensis</em> (L)<em>.</em> A yield of 0.44 ± 0.02 % essential oil was obtained from dry rhizomes. Gas chromatography-mass spectrometry (GC-MS) characterization revealed the major chemical constituents as 1,8-cineole (25.71 %), ethyl iso-allocholate (16.01 %), and fenchyl acetate (15.15 %). The findings from the investigation into the pesticidal characteristics of essential oils derived from the rhizome of <em>A. calcarata</em> unveiled significant contact (LC₅₀ at 24 h was 10.9 mg/cm² for <em>T. castaneum</em>, 1.0 mg/cm² for <em>L. serricorne</em> and 0.5 mg/cm² for <em>C. chinensis</em>) and fumigant toxicity (LC₅₀ at 24 h was 4.05 mg/L air for <em>T. castaneum</em>, 1.75 mg/L air for <em>L. serricorne,</em> and 1.03 mg/L air for <em>C. chinensis</em>). The repellent efficacy was evaluated through an area preference test, revealing robust repellent activity at various concentrations. Undoubtedly, the essential oil extracted from the rhizome of <em>A. calcarata</em> is valuable and has the potential to be a safe, organic pesticide for controlling storage pests.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100088"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000232/pdfft?md5=a783047aac7fe8f2713818ac95ba3d1f&pid=1-s2.0-S2773078624000232-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141622397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Larvicidal, bactericidal, antioxidant, hypoglycemic effects and anti-proliferative activity of phyto-synthesized Ag-ZnO nanocomposites using Eranthemum roseum (Vahl) R.Br. leaf extract against Aedes aegypti and Culex quinquefasciatus mosquito vectors","authors":"Ramachandran Adhavan ,&nbsp;Kuppusamy Selvam ,&nbsp;Palanisamy Prakash ,&nbsp;Dharmaligam Kirubakaran ,&nbsp;Muthugounder Subaramanian Shivakumar","doi":"10.1016/j.napere.2024.100093","DOIUrl":"10.1016/j.napere.2024.100093","url":null,"abstract":"<div><p>This research is conducted using zinc doped silver nanocomposite on <em>Eranthemum roseum</em> leaf extract. <em>E. roseum</em> has its own medicinal properties due its bioactive compounds. Silver and Zinc also has its own medicinal properties due to its microbial properties. Our work is aimed to combine nanoparticles with plant extract to explore the medicinal properties. The synthesized Ag-ZnO NCs is characterized using X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), Scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDAX) and Transmission Electron Microscopy (TEM) analysis. The XRD analysis confirms the crystal nature of the Ag-ZnO NCs. The average shape of the nanocomposite was obtained using SEM as spherical, EDAX analysis confirming the presence of Ag, Zn and Oxygen and TEM conforms the oval shape of nanoparticles Antibacterial activity shows highest level of activity against <em>K. pneumoniae</em> and <em>B. subtilis</em>. DPPH Activity of Ag-ZnO NCs highest inhibition of 52.56 % while the ABTS analysis of Ag-ZnO NCs shows 54.28 %. Hypoglycemic activity α-amylase and α- glucosidase assay of Ag-ZnO NCs obtained good activity with maximum level of inhibition 63 % and 60 %. Anti-proliferative assay shows good activity against cancer causing cells in HepG2 human liver cancer line with 67.11 %. Larvicidal potential of Ag-ZnO NCs recorded good mortality rate against <em>Aedes aegypti</em> and <em>Culex quinquefasciatus.</em> This study confirms the maximum activity of Ag-ZnO NCs and acts as Larvicidal, Anti-proliferative, Antioxidant, Hypoglycemic agents.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100093"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000281/pdfft?md5=97a8462a8fc7af039f2562c76998e00f&pid=1-s2.0-S2773078624000281-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141952736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PyPI: An internet-enabled learning tool to boost learner motivation for crop protection","authors":"Yoshiyasu Takefuji","doi":"10.1016/j.napere.2024.100089","DOIUrl":"10.1016/j.napere.2024.100089","url":null,"abstract":"<div><p>Instructors are always interested in methods to activate learner incentives and motivation to increase learning effectiveness. This paper introduces the Python Package Index (PyPI) as a powerful tool to maximize learner incentives on software and presents an example of its application in agriculture and science. The more useful the PyPI application is, the more it will be downloaded worldwide, providing an external review for the learner, and strengthening their incentive. However, many existing tutorials on PyPI, including the official site, are not updated on the twine library for uploading files to the PyPI site. This paper presents an updated tutorial on using PyPI for counting disaggregated objects such as bugs and pests, and for software reproducibility validation via Code Ocean. Additionally, generative AI is introduced as an indispensable assistant for tasks such as understanding technical terms and providing solutions for encountered problems.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"10 ","pages":"Article 100089"},"PeriodicalIF":0.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000244/pdfft?md5=d8bf16af3f845d5a10d0045d141391c1&pid=1-s2.0-S2773078624000244-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141692751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-black pod disease activity of leaf extract of Carica papaya Linn","authors":"Tamekloe Woasiedem ,&nbsp;Ishmael Amoako-Attah ,&nbsp;Francis Ackah Armah ,&nbsp;Isaac Asiamah","doi":"10.1016/j.napere.2024.100082","DOIUrl":"10.1016/j.napere.2024.100082","url":null,"abstract":"<div><h3>Background</h3><p><em>Theobroma cacao (</em>Cocoa) is a major foreign exchange earner for Ghana. Over the recent decades, there has been a consistent decline in cocoa production in the country. A major contributing factor is the cocoa black pod disease caused by <em>Phytophthora</em> species. Synthetic fungicides have been used to control the pathogens over the years. However, recent calls for organic cocoa devoid of residues from synthetic fungicides, which are known to pose several health challenges as well as a negative impact on the environment, have gained attention. Thus, inviting investigations into more environmentally friendly and sustainable alternatives to synthetic fungicides.</p></div><div><h3>Objective</h3><p>This study explored the inhibitory potential of crude extract from <em>Carica papaya</em> against cocoa black pod disease caused by <em>Phytophthora</em> spp.</p></div><div><h3>Material and methods</h3><p>An <em>in-silico</em> study was done using known phytochemicals from <em>Carica papaya</em> as ligands against a <em>Phytophthora</em> effector protein. Crude extracts were obtained from the plant materials using 70 % ethanol as the extraction solvent. The crude Green Leaf Extract (GLE), Aging Induced Chlorophyll Deficient Leaf Extract (AICDLE), and Matured Black Seed Extract (SDE) were tested <em>in-vitro</em> against pre-cultured <em>P. palmivora</em> and <em>P. megakarya</em> using the poison food technique. GLE was modulated with Delco, a synthetic fungicide, at different ratios which were tested <em>in-vitro</em> and <em>in-vivo</em>.</p></div><div><h3>Results</h3><p>A total of 16 ligands showed binding affinity higher or equal to the standard ligand, alliin, suggesting <em>Carica papaya</em> as a repository of anti-phytophthora agents. Assessment of crude extracts at concentrations 5–20 mg/ml showed fungistatic activity against <em>P. palmivora</em> and <em>P. megakarya in-vitro</em>. Modulation of GLE with Delco produced some combinations that made GLE fungicidal <em>in-vitro</em> against <em>P. palmivora</em> and <em>P. megakarya</em> with fractional inhibition concentration indices between 0.51 and 0.65, interpreted as partially synergistic. The <em>in-vivo</em> assessment of combination D (Delco and GLE in a 0.3 mg:3 mg per ml ratio) performed as well as Delco alone at the recommended dose of 5 mg/ml. Thus, combination D reduces the requirement for copper-based fungicide by over 90 %.</p></div><div><h3>Conclusion</h3><p>This study shows <em>Carica papaya</em> in a new light as a potential material that could be formulated into a natural product-based fungicide for combating <em>Phytophthora</em>-induced black pod disease.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"9 ","pages":"Article 100082"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000177/pdfft?md5=f2f79eabd8bcdc8aa7d3dd0aaac17ec4&pid=1-s2.0-S2773078624000177-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141047631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Some aspects concerning citronella grass essential oil and the agroecological approach to protecting stored cowpea beans","authors":"Marcela de Souza Alves ,&nbsp;Elisabeth Alves Duarte Pereira de Medeiros ,&nbsp;Camila da Silva Barbosa Pereira ,&nbsp;Cristiane Martins Cardoso ,&nbsp;Emerson Guedes Pontes ,&nbsp;André Marques dos Santos ,&nbsp;Marco Andre Alves de Souza","doi":"10.1016/j.napere.2024.100084","DOIUrl":"https://doi.org/10.1016/j.napere.2024.100084","url":null,"abstract":"<div><p>This study aimed to determine the impact of hydrodistillation duration on the chemical composition of citronella-grass essential oil. Furthermore, the oil's efficacy against cowpea weevil was evaluated by carrying out biological activity tests. The essential oil was obtained and chemically characterized. Subsequently, it was tested at different concentrations to evaluate its impact on the reproductive cycle and the total activity of the insects' biotransformation enzymes. Oxygenated monoterpene comprised the majority of the substances present. The mathematical model fitted to the extraction kinetics predicts that after 60 minutes of hydrodistillation, approximately 96% of essential oil, 94% of monoterpenes, and 50% of sesquiterpenes will be obtained with confidence. The fumigating effect of the citronella-grass essential oil resulted in increased mortality, reduced oviposition, and lower emergence of adults. The essential oil at sublethal concentration (0.09 mg cm^-3 for 6 hours) significantly reduced the total activity of biotransformation enzymes (acetylcholinesterase, α- and β-esterase, carboxylesterase) in female cowpea weevils.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"9 ","pages":"Article 100084"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000190/pdfft?md5=94f2e0f4b0c29d471d1be1b5845f26f3&pid=1-s2.0-S2773078624000190-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141243612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilization of herbivore defensive latex from the weed Calotropis procera L in the green synthesis of silver nanoparticles and its potential application in the control of dengue vector Aedes aegypti","authors":"B.R. Harisma ,&nbsp;Christy J. Jenifer Annis ,&nbsp;S. Benazir Begum ,&nbsp;R. Kalpana ,&nbsp;Murugappan Rm","doi":"10.1016/j.napere.2024.100073","DOIUrl":"10.1016/j.napere.2024.100073","url":null,"abstract":"<div><p>Milky latex produced by few and not all the plants are the treasure chest of various bioactive compounds with defensive potential against herbivores, insects and pathogens. Mankind have utilized latices in the preparation of novel plant-based therapies for centuries. Therefore, an attempt has been made to synthesis silver nanoparticles (AgNPs) using AgNO₃ as a precursor and latex from <em>C. procera</em> as a reducing agent. The surface plasmon absorption peak at 430 nm confirmed AgNPs formation. The sharp peak with narrow width illustrated the homogenous nature. The face centre cubic structure of AgNPs was confirmed by the XRD pattern. FT-IR analysis deduced that the bio-molecules in the latex of <em>C. procera</em> are responsible for the reduction of AgNO₃ to AgNPs. Biosynthesised L-AgNPs possessed well pronounced antibacterial activity against <em>P. aeruginosa</em> and <em>K. pneumoniae</em>. Anti-inflammatory activity of the nanoparticle determined by membrane stabilization and proteinase inhibitory assay was found to be in dose-dependent. LC₅₀ value of L-AgNPs was found to be 63.09 µg/mL against IV instar larvae of <em>A. aegypti</em>. Distinct morphological changes were observed in the mosquito larva on exposure to various concentrations of L-AgNPs.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"9 ","pages":"Article 100073"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000074/pdfft?md5=97ec69065a1e355a7424fab5119579e1&pid=1-s2.0-S2773078624000074-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140401936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical screening and antimicrobial activities of Guizotia abyssinica L. leaf and flower extracts","authors":"Priyanka Prasad ,&nbsp;Aadil Mansoori ,&nbsp;Nisha Prajapati ,&nbsp;Jyotima Tripathi ,&nbsp;Kapil Sharma ,&nbsp;Anirudh Kumar ,&nbsp;Subha Narayan Das","doi":"10.1016/j.napere.2024.100083","DOIUrl":"https://doi.org/10.1016/j.napere.2024.100083","url":null,"abstract":"<div><p>Sustainable agricultural systems demand less or no use of synthetic chemicals, which have various adverse effects on the environment and human health. In this context, studies on phytochemicals with antimicrobial and antioxidant properties hold great promise to suppress plant diseases and, thereby, reducing the application of synthetic chemical pesticides. Such an alternative approach requires screening and identification of bioactive compounds from various plant species. In the present study, <em>Guizotia abyssinica</em> L., an oilseed crop plant that usually requires less water with no pesticide inputs to grow, was screened for its potential antioxidant and antimicrobial phytochemicals. The methanolic and aqueous extracts of leaves and flowers of <em>G. abyssinica</em> were tested for their polyphenol content (total phenols and flavonoids) and antioxidant potential. The antioxidant and free radical scavenging activities of both methanolic and aqueous extracts were higher in comparison to many previously reported plant extracts. Furthermore, leaf extracts of <em>G. abyssinica</em> have shown slightly higher antioxidant activity than flower extracts. <em>In vitro</em> antimicrobial studies confirmed the inhibitory effect of methanolic extracts of leaves and flowers against phytopathogens like <em>Xanthomonas oryzae</em> RR24, <em>Fusarium</em> sp. and <em>Alternaria</em> sp. The chemical nature and composition of these crude extracts were analyzed by Fourier Transform Infrared Spectroscopy (FTIR) and Gas Chromatography Mass Spectrometry (GC-MS). Approximately, 28 bioactive compounds detected in GC-MS were predicted to have antimicrobial activity. Based on our results, we conclude that the extracts derived from <em>G. abyssinica</em> have demonstrated remarkable efficacy in inhibiting the proliferation of phytopathogens, thus exhibiting immense potential in promoting sustainable agricultural practices.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"9 ","pages":"Article 100083"},"PeriodicalIF":0.0,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2773078624000189/pdfft?md5=c351619151207cfe6d1cb8c3b2ba1906&pid=1-s2.0-S2773078624000189-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140918823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}