Journal of Natural Pesticide Research最新文献

{"title":"Efficacy of Trichoderma harzianum, as a biological fungicide against fungal diseases of potato, late blight and early blight","authors":"Md. Mahi Imam Mollah ,&nbsp;Nayem Hassan","doi":"10.1016/j.napere.2023.100047","DOIUrl":"https://doi.org/10.1016/j.napere.2023.100047","url":null,"abstract":"<div><p>This study was conducted at Rangpur area of Bangladesh to evaluate the performance of <em>Trichoderma harzianum</em> based bio-fungicide against late blight and early blight of potato. A commercial fungicide, Agrizeb (Mancozeb) 80 WP was applied for comparison. These fungicides were applied in potato filed already infected by late and early blight. Lycomax found most effective for controlling both late blight and early blight while Agrizeb found potent for early blight. After applying Lycomax, late blight infection in plant has not increased rather decreased to 20.0% from the existing 22.67% and leaf infestation reduced to 10.67% from the existing 13.33%. In contrast for Agrizeb, plant infection increased to 71.67% from the prevailing 22.0% and leaf infection increased to 73% from the existing 14.67%. Thus, Lycomax confirmed 79.09% reduction of plant infection and 89.04% reduction of leaf infection compared to control which was 25.09% and 25%, respectively for Agrizeb. For early blight, same dose of Lycomax also reduce plant infection to 1.67% from existing 14.67% and leaf infection to 8.0% from prevailing 13.33%. Plant infection decreased to 5.0% from existing 15.0% and leaf infection 13.33% from 14.67% for Agrizeb. Therefore, Lycomax provided 98.19% control of plant infection and 90.16% control of leaf infection while Agrizeb provided 94.58% plant infection and 83.61% leaf infection over control. Moreover, Lycomax confirmed 21.66 ton/ha and 22.0 ton/ha of potato yield recovering the late blight and early blight diseases, respectively. These results conclude that Lycomax can effectively be applied to control late blight and early blight diseases.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"5 ","pages":"Article 100047"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49727362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"4-Hydroxybenzyl alcohol from Sinapis alba mustard seed meal extract for control of liverwort (Marchantia polymorpha)","authors":"Inna E. Popova ,&nbsp;Jeremiah Dubie ,&nbsp;Matthew J. Morra","doi":"10.1016/j.napere.2023.100043","DOIUrl":"https://doi.org/10.1016/j.napere.2023.100043","url":null,"abstract":"<div><p><em>Sinapis alba</em> mustard seed meal extract is a by-product of mustard oil extraction, that is used for biodiesel and renewable jet fuel production. Recently, <em>S. alba</em> mustard seed meal has been shown to have herbicidal activity for a range of problematic weeds. The objectives of the present research were 1) to optimize the preparation of <em>S. alba</em> mustard seed meal extract; 2) to explore the applicability of <em>S. alba</em> mustard seed meal extract for control of liverwort, a common pest in ornamental plants greenhouses; and 3) to identify the major herbicidal compound(s) in these extracts. For extraction, methanol content in aqueous solution was optimized between 0% and 100%. Extracts prepared with 0–30% aqueous methanol provided 98–100% control of liverwort after single application and prevented liverwort plants from recovering. Extracts prepared using sequential extraction with 60% methanol provided an additional benefit of low particulate solutions. The efficiency of the produced <em>S. alba</em> mustard seed meal extract was comparable to two commercially available herbicides labeled for liverwort control under greenhouse conditions. Composition of <em>S. alba</em> mustard seed meal extracts was analyzed by high-performance liquid chromatography mass spectrometry and ion chromatography. 4-Hydroxybenzyl alcohol was identified as a main herbicidal compound based on the analysis and using commercially available reference solution. To the best of our knowledge, this is the first report of 4-hydroxybenzyl alcohol herbicidal activity toward nonvascular plants. The high concentrations of 4-hydroxybenzyl alcohol naturally produced from <em>S. alba</em> mustard seed meal, makes it a potential herbicide feedstock material.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"5 ","pages":"Article 100043"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49752548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal activity of Leonotis nepetifolia extracts against Colletotrichum species causing bean anthracnose and their phytochemical analysis using LC-MS","authors":"Faustine Christopher ,&nbsp;Patrick Ndakidemi ,&nbsp;Samuel Nyalala ,&nbsp;Ernest Mbega","doi":"10.1016/j.napere.2023.100057","DOIUrl":"https://doi.org/10.1016/j.napere.2023.100057","url":null,"abstract":"<div><p>Bean anthracnose results in significant yield losses, and the present primary management strategy of using synthetic fungicides is hampered by a number of factors. This study assessed the antifungal effects of aqueous, methanolic, and ethyl acetate extracts of <em>Leonotis nepetifolia (Lamiaceae)</em> against <em>Colletotrichum</em> species that cause bean anthracnose. The poisoned food technique was used to evaluate extracts in-vitro, and trials were carried out on farmer's plots. On the other hand, phytochemical analysis was done using LC-MS. Results obtained in-vitro show that the ethyl acetate extract was the most effective since it completely inhibited mycelial development at concentrations of 5 mg/mL and 2.5 mg/mL. On the other hand, the aqueous and methanolic extracts exhibited a comparable inhibitory effect, and at the higher concentration (5.0 mg/mL), a remarkable percentage of inhibition of 73.3% and 83.1% was recorded. According to field trials, bean anthracnose incidence was low and there were fewer infected pods per plant in plots treated with <em>L. nepetifolia</em> extracts than in the negative control. In comparison to the negative control plots, extracts-treated plots had larger weights of 100 seeds but no discernible negative effect in seed germination. LC-MS analysis indicated the presence of Linoleoyl ethanolamide, Curcumol, Deguelin, 9,10-dihydroxy-12Z-octadecenoic acid, Naringenin-7-O-glucoside, and 9S-hydroxy-10e 12z 15z-octadecatrienoic acid in the ethyl acetate extract whereas 6-Methoxyluteolin, Genistein, 5,7,3,4-tetramethoxyisoflavone, and 3,4-Dihydroxymandelic acid were identified in the aqueous extract. Antifungal activity and the presence of bioactive components revealed by extracts in this study demonstrate the potential of <em>L. nepetifolia</em> as a source of botanical fungicide for the management of bean anthracnose.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"6 ","pages":"Article 100057"},"PeriodicalIF":0.0,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49752938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Users opinion about synthetic, bio- and nano-biopesticides","authors":"S. Sreevidya ,&nbsp;Kirtana Sankarasubramanian ,&nbsp;Yokraj Katre ,&nbsp;Sushma Yadav ,&nbsp;Anupama Asthana ,&nbsp;Ajaya Kumar Singh ,&nbsp;Frank Alexis ,&nbsp;Sónia A.C. Carabineiro","doi":"10.1016/j.napere.2023.100058","DOIUrl":"https://doi.org/10.1016/j.napere.2023.100058","url":null,"abstract":"<div><p>With a growing global population, the demand for food, clothing and shelter became crucial for sustaining life. Moreover, the COVID-19 pandemic/endemic, along with the threat of other endemics, posed significant challenges in ensuring the delivery of nutritionally rich food. To address this, the World Health Organization (WHO) recommended the ban of some highly toxic chemical pesticides. Excessive consumption and exposure to pesticides possibly contributed to a decline in the human immunity levels making our fight against the pandemics more difficult and challenging. During the lockdown COVID-19 crisis, we conducted a survey to gather insights from farm landers (FLs), garden lovers (GLs), domestic front users (DFUs) predominantly from India, but also from other regions worldwide. The survey aimed at better understanding the usage of pesticides, both chemicals (CPs), bio-pesticides (BPs) or both, and their global utilization. A statistical survey with seven rudimentary sections was designed to receive the inputs (elementary inputs, awareness and perception, pesticide utility, health, ecology, and alternatives for safer trials) with fairness and care. The data was statistically analyzed within each group of population, revealing significant variations within the groups. Our study indicated that FLs (44.7 %) predominantly used BPs, GLs (18.8 %) relied on CPs, while DFUs (100.0 %) used a combination of both without any specific intention. Correlation analysis, given by R (correlation coefficient) and p (probability of obtaining an equal or more extreme effect than the found considering the null hypothesis as true) values revealed the existence of a positive and significant relationship between the selected variables, such as level of education (LOE) and gender, with the adoption of new alternatives (2loglikelihood = 64.743, with χ² = 128.4, degrees of freedom (df) = 24, p&lt;*0.05, **0.01, and *** 0.001) through multinomial regression analysis, indicating the fitness of the model. Likewise, our study primarily focused on exploring the insights for the development of nano-biopesticides, as improved alternatives to the existing solutions.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"6 ","pages":"Article 100058"},"PeriodicalIF":0.0,"publicationDate":"2023-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49753120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemo-Profiling and Biochemical Implications of using 9, 12-Octadecadienoic acid, from Ethanol Leaf Extract of Anacardium occidentale (Cashew) for Bio-pesticide Purposes.","authors":"C. Okonkwo, Jesam Uket, S. Okonkwo, Favour Ubio","doi":"10.1016/j.napere.2023.100056","DOIUrl":"https://doi.org/10.1016/j.napere.2023.100056","url":null,"abstract":"","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90538573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of essential oils to control some important fungi and bacteria pathogenic on cereals","authors":"Parissa Taheri,&nbsp;Marjan Soweizy,&nbsp;Saeed Tarighi","doi":"10.1016/j.napere.2023.100052","DOIUrl":"https://doi.org/10.1016/j.napere.2023.100052","url":null,"abstract":"<div><p>Among the poaceae family, cereals are known as the most important products because they are the main source of energy and food. Different plant pathogens, including oomycetes, fungi, bacteria, nematodes and viruses, can infect cereal products. Fungal diseases are the most important group of cereal pathogens, causing significant crop losses worldwide. Several techniques, including chemical control, the use of resistant cultivars, biological control, and application of antimicrobial plant metabolites can be used to manage cereal diseases. The use of chemical products is limited due to undesirable environmental effects and the emergence of resistant pathogens to fungicides. Due to the fact that medicinal plants produce a variety of secondary metabolites with antimicrobial effects, they are considered as an alternative to chemical substances. In this study, we have discussed the sensitivity of most important fungal and bacterial pathogens of cereal crops against different essential oils and their main components, together with their modes of action in controlling cereal diseases.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"6 ","pages":"Article 100052"},"PeriodicalIF":0.0,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49752728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacillus thuringiensis increases the efficacy of bio-pesticides against eggplant shoot and fruit borer, Leucinodes orbonalis Guenee","authors":"Md. Mahi Imam Mollah ,&nbsp;Nayem Hassan ,&nbsp;Soyema Khatun ,&nbsp;Md. Mahbubar Rahman","doi":"10.1016/j.napere.2023.100055","DOIUrl":"https://doi.org/10.1016/j.napere.2023.100055","url":null,"abstract":"<div><p>Brinjal shoot and fruit borer (BSFB) is a notorious pest of brinjal. This study evaluates some microbe and plant origin bio-pesticides alone or in combination with <em>Bacillus thuringiensis</em> var. kurstaki 5 % (<em>Btk</em> 5 %) in the brinjal field to combat against BSFB. We found that application of biopesticides in combination with <em>Btk</em> 5 % increased the control of BSFB that results increased marketable yield, gross yield, individual fruit weight compared to their application without <em>Btk</em> 5 %. Among the biopesticide combinations, Abamectin 1.2 % + Emamectin benzoate 1 % and <em>Btk</em> 5 % as well as Emamectin benzoate 1 % and <em>Btk</em> 5 % were potent in context to BSFB control and yield attributes. These combinations confirmed 78.04 % and 74.76 % shoot infestation control, 70.90 % fruit infestation reduction in number basis, 72.40 % and 72.18 % fruit infestation control in weight basis, 13.56 ton ha⁻¹ and 11.41 ton ha⁻¹ marketable yield, 15.59 ton ha⁻¹ and 12.37 ton ha⁻¹ gross yield, respectively. In contrast, Abamectin 1.2 % + Emamectin benzoate 1 % and Emamectin benzoate 1 % confirmed 58.18 % and 67.96 % shoot infestation control, 50.27 % and 62.06 % fruit infestation reduction in number basis, 51.86 % and 59.24 % fruit infestation control in weight basis, 9.05 ton ha⁻¹ and 9.44 ton ha⁻¹ marketable yield, 11.82 ton ha⁻¹ and 10.83 ton ha⁻¹ gross yield. These promising bio-pesticide combined with Bt also ensure more individual fruit weight compared to without Bt. These results thus illustrated that application of <em>Btk</em> 5 % along with bio-pesticide increases their efficacy because of the synthesis of insecticidal toxins named Cry, Vip, and Cyt by <em>Btk</em> 5 % that have synergistic effect with biopesticides and kill insects.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"6 ","pages":"Article 100055"},"PeriodicalIF":0.0,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49752726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potency and effectiveness of six essential oils in controlling grapevine trunk diseases in Morocco","authors":"Jihane Kenfaoui ,&nbsp;Rachid Lahlali ,&nbsp;Salah-Eddine Laasli ,&nbsp;Khadija Goura ,&nbsp;Majda Fardi ,&nbsp;Abdessalem Tahiri ,&nbsp;Lahsen El Ghadraoui ,&nbsp;Said Amiri","doi":"10.1016/j.napere.2023.100053","DOIUrl":"https://doi.org/10.1016/j.napere.2023.100053","url":null,"abstract":"<div><p>The objective of this study was to evaluate the antifungal activity (AA) of essential oils (EOs) extracted from <em>Eucalyptus globulus</em> Labill. (Eucalyptus), <em>Citrus limonum</em> (L.) Burm. (Lemon), <em>Cinnamomum zeylanicum</em> Blume. (Cinnamon), <em>Lavandula latifolia</em> aspic (Lavender), <em>Rosmarinus officinalis</em> L. (Rosemary), and <em>Mentha spicata</em> L. (Peppermint) against four pathogenic fungi responsible for grapevine trunk diseases in Moroccan vineyards. The EOs with the highest <em>in vitro</em> efficacy were selected for further testing on autoclaved grape wood assays against <em>Diplodia mutila</em>, <em>Neoscytalidium novaehollandiae</em>, <em>Trichothecium roseum</em>, and <em>Neopestalotiopsis vitis</em>. The major chemical constituents of the tested EOs were determined through GasChromatography–Mass Spectrometry (GC-MS) analysis, and their relative percentages were recorded. All tested EOs demonstrated significant antifungal activity against the four pathogens, as evidenced by <em>in vitro</em> results. Redundancy analysis (RDA) confirmed the efficacy of the tested EOs against grapevine fungal pathogens. The efficacy of each EO varied depending on the pathogen being targeted. For example, lavender had the highest efficacy across EOs (81.37%) against <em>T. roseum</em>, while peppermint was the most effective in inhibiting <em>D. mutila</em> (47.45%). Cinnamon was the most effective EO in controlling <em>N. vitis</em> (73.33%), while rosemary had the best effect in controlling <em>N. novaehollandiae</em> (56.79%), with the most effective EC₅₀ value (8.66 µL/ml) being attributed to the rosemary EO. The <em>in vitro</em> results were further confirmed through autoclaved grape wood assays, with inhibition of proliferation ranging between 93.14% and 66.32%. These findings represent a novel application of EOs extracted from various medicinal plants to control fungal diseases in grapevine plantations.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"6 ","pages":"Article 100053"},"PeriodicalIF":0.0,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49766006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential of entomopathogenic nematodes for the management of the mirid bugs Lygus rugulipennis (Poppuis), Liocoris tripustulatus (Fabricius) and Macrolophus pygmaeus (Rambur)","authors":"Emile Steenman ,&nbsp;Ernest Ireneusz Hennig ,&nbsp;Gaëtan Jaccard ,&nbsp;Elena Mihailescu ,&nbsp;Serge Fischer ,&nbsp;Louis Sutter","doi":"10.1016/j.napere.2023.100054","DOIUrl":"https://doi.org/10.1016/j.napere.2023.100054","url":null,"abstract":"<div><p>Mirid bugs (Hemiptera: Miridae) represent a significant challenge for greenhouse cash crops like cucumber, tomato and eggplants, leading to huge economic losses. This study investigated the potential of the entomopathogenic nematode (EPN) <em>Steinernema carpocapsae</em> as biological control agent of the mirid bug species <em>Lygus rugulipennis</em>, <em>Liocoris tripustualtus</em>, and <em>Macrolophus pygmaeus</em> through aerial spray application under laboratory and greenhouse conditions (only for <em>L. rugulipennis</em> and <em>M. pygmaeus</em>). The laboratory trials showed a significant effect of <em>S. carpocapsae</em> on the average survival rate of the three mirid species. The highest efficacy was found for subadults of <em>L. rugulipennis</em> (50%), followed by <em>M. pygmaeus</em> (25%) and <em>L. tripustulatus</em> (15%). Microscopic dissections showed that EPNs can infect all studied mirid species and life stages. Under greenhouse conditions, a significant difference was observed between developmental stages, with no significant effect for adults, but an efficacy of 19% and 32% for nymphs of <em>L. rugulipennis</em> and <em>M. pygmaeus</em>, respectively. These results highlight the potential of EPNs in the control of problematic mirid bugs in greenhouse vegetable production, which could lead to a reduction in the use of synthetic pesticides and promote more sustainable agricultural practices.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"6 ","pages":"Article 100054"},"PeriodicalIF":0.0,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49765999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of microbial antagonists in the management of bacterial wilt of field-grown tomato","authors":"Arthur O. Wamani ,&nbsp;James W. Muthomi ,&nbsp;Eunice Mutitu ,&nbsp;Wanjohi J. Waceke","doi":"10.1016/j.napere.2023.100051","DOIUrl":"https://doi.org/10.1016/j.napere.2023.100051","url":null,"abstract":"<div><p>Bacterial wilt caused by <em>Ralstonia solanacearum</em> can cause up to 100% yield loss in tomato production and has compelled many farmers to abandon previous productive farms. Consequently, the absence of an effective control method demands an intensive such for a functional management option. The study was carried out to evaluate the efficacy of microbial antagonists in managing bacterial wilt under field conditions. The experiment was laid down under Randomized Complete Block Design and four replicates maintained for each treatment. Antagonistic strains of locally isolated <em>Trichoderma hamatum</em>, <em>T. atroviride</em>, <em>T. harzianum</em>, <em>Bacillus subtilis</em>, <em>Serratia</em> spp., and <em>Acinetobacter</em> spp. were assessed. Commercial formulations of <em>T. viride</em> (Bio Cure F®) and <em>Pseudomonas fluorescence</em> (Bio Cure B®) were included as standard checks. Treatment application was initiated at transplanting by drenching the rhizosphere with 50 ml of the inoculum. Application was repeated every two weeks until tenth week after transplanting. Data was collected on plant stand count, disease incidence, disease severity, plant height, plant biomass, and fruit weight. <em>Trichoderma hamatum</em> was the most effective in experiment 1, reducing crop mortality, incidence and AUDPC by up to 51.7%, 49.3% and 58.2%, respectively. It also exhibited the highest percentage yield increase by up to 196.4% compared to the untreated control. In contrast, <em>B. subtilis</em> showed superior disease suppression in experiment 2, reducing crop mortality, incidence and AUDPC by up to 44.6%, 48.5% and 51.0%, respectively. It also increased biomass by approximately 62.0% in both experiments. It was closely followed by <em>Serratia</em> spp., which also gave the highest yield output in experiment 2 of up to 233.0%. These findings suggest that novel antagonistic strains <em>T. hamatum</em> and <em>B. subtilis</em> presents unique opportunities for exploiting local microbial resources as biocontrol products. These products can be incorporated as part of an integrated bacterial wilt management program in farms where production is significantly affected by the disease.</p></div>","PeriodicalId":100809,"journal":{"name":"Journal of Natural Pesticide Research","volume":"6 ","pages":"Article 100051"},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49753221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}