Biotechnology Research and Innovation最新文献_第8页

Isolation, characterization and identification of extracellular enzyme producer Bacillus licheniformis from municipal wastewater and evaluation of their biodegradability 城市污水中胞外产酶物地衣芽孢杆菌的分离、鉴定及可降解性评价

Biotechnology Research and Innovation Pub Date : 2018-01-01 DOI: 10.1016/j.biori.2018.03.001

Nilesh Sonune , Anil Garode

{"title":"Isolation, characterization and identification of extracellular enzyme producer Bacillus licheniformis from municipal wastewater and evaluation of their biodegradability","authors":"Nilesh Sonune , Anil Garode","doi":"10.1016/j.biori.2018.03.001","DOIUrl":"https://doi.org/10.1016/j.biori.2018.03.001","url":null,"abstract":"<div>In the present investigation, a total of 34 bacterial isolates were isolated from municipal wastewater and sludge samples. Out of 34, 5 isolates were selected based on their growth on wastewater agar medium and were identified on the basis of morphological, cultural and biochemical tests. The enzyme production study showed that all the isolates were able to produce lipase and protease while one isolate (NW1D) showed amylase production. The bioremediation potential of these enzymes producing bacteria was investigated and the results suggest that the isolate NW6 was most efficient for bioremediation and showed reduction in biochemical oxidation demand (BOD), chemical oxidation demand (COD), nitrate and phosphate by 50.65%, 20%, 56.25% and 31.13% respectively after 72h. Further, the NW6 isolate identified as Bacillus licheniformis by 16S rRNA sequencing and was optimized for different parameters to achieve effective results. The results showed that overall optimum inoculum size, retention time and agitation speed for NW6 were 10% (v/v), 96h and 200rpm respectively. The reduction in BOD, COD, nitrate and phosphate was found to be 54.55%, 36%, 24.83% and 26.42% respectively with 10% inoculum size. At 96h retention time with 10% inoculum, BOD, COD, nitrate and phosphate reduction was 69.16%, 42.5%, 49.89% and 39.62% respectively. The reduction in BOD, COD, nitrate and phosphate was found to be 72.08%, 51%, 51.87% and 31.13% respectively at 200rpm with 10% inoculum. Further, the NW6 may find application in municipal wastewater treatment to prevent water pollution as well as a potential enzyme production.</div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"2 1","pages":"Pages 37-44"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2018.03.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91759966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Mining and analysis of simple sequence repeats in the chloroplast genomes of genus Vigna 维纳属叶绿体基因组简单重复序列的挖掘与分析

Biotechnology Research and Innovation Pub Date : 2018-01-01 DOI: 10.1016/J.BIORI.2018.08.001

N. Shukla, Himani Kuntal, A. Shanker, S. Sharma

引用次数: 15

Candida tropicalis able to produce yeast single cell protein using sugarcane bagasse hemicellulosic hydrolysate as carbon source 热带假丝酵母能够以蔗渣半纤维素水解物为碳源生产酵母单细胞蛋白

Biotechnology Research and Innovation Pub Date : 2018-01-01 DOI: 10.1016/j.biori.2018.08.002

Cristina Emanuela Barbosa Magalhães , Manoel Santiago Souza-Neto , Spartaco Astolfi-Filho , Italo Thiago Silveira Rocha Matos

{"title":"Candida tropicalis able to produce yeast single cell protein using sugarcane bagasse hemicellulosic hydrolysate as carbon source","authors":"Cristina Emanuela Barbosa Magalhães , Manoel Santiago Souza-Neto , Spartaco Astolfi-Filho , Italo Thiago Silveira Rocha Matos","doi":"10.1016/j.biori.2018.08.002","DOIUrl":"10.1016/j.biori.2018.08.002","url":null,"abstract":"<div>The world demand for foods will be the most important bottle-neck for human being maintenance. In this context, the research for alternative proteins sources aiming human and animal feeding is a necessary approach. A wild-type strain of Candida tropicalis (KP276650) was identified as potential yeast single cell protein (YSCP) producer using sugarcane bagasse hemicellulosic hydrolysate (SBHH) as substrate. Were evaluated the cell growth and sugar consumption along fermentation assay. Biomass yield (YB, gg−1), protein percentage (%), protein yield (YP, gg−1) and yeast biomass productivity (YBP, gL−1        h−1. Percentage of protein in yeast biomass produced was 60.05%, biomass yield (YB) and protein yield (YP) were, respectively, 0.28 and 0.17gg−1. The YB and YP obtained are close to the observed for some industrial strains of yeasts. The percentage of protein is greater than the expected for most of yeasts and fungi. These results indicate that the using of C. tropicalis (KP276650) for YSCP production may to aggregate high value for sugarcane bagasse.</div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"2 1","pages":"Pages 19-21"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2018.08.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91218548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Phytochemical constituents of Dracaena mahatma leaves and their anti-bacterial, anti-oxidant and anti-inflammatory significance 龙血树叶的植物化学成分及其抗菌、抗氧化和抗炎意义

Biotechnology Research and Innovation Pub Date : 2018-01-01 DOI: 10.1016/j.biori.2018.09.002

Saranya Shankar , Sugashini Settu , Gayathri Segaran , Ranjitha Dhevi V. Sundar , Lokesh Ravi

{"title":"Phytochemical constituents of Dracaena mahatma leaves and their anti-bacterial, anti-oxidant and anti-inflammatory significance","authors":"Saranya Shankar , Sugashini Settu , Gayathri Segaran , Ranjitha Dhevi V. Sundar , Lokesh Ravi","doi":"10.1016/j.biori.2018.09.002","DOIUrl":"https://doi.org/10.1016/j.biori.2018.09.002","url":null,"abstract":"<div>The present study aims to analyze the antimicrobial, antioxidant and anti-inflammatory activity of Dracaena mahatma leaf extract. Qualitative phytochemical screening of leaf powder confirmed the presence of tannins, alkaloids, phenols, terpenoids, sterols, triterpenes and anthraquinone glycosides. GC-MS analysis of methanolic extract suggested the presence of 2 unknown compounds and 8 known phytochemicals, of which benzene-(1,6-hexanediylidene)tetrakis constituted a maximum percentage of the crude extract. The total phenolic content of the crude extract was found to be 32.9±0.002GAE/g. Crude extract demonstrated 90% antioxidant activity in DPPH assay at 100μg/ml concentration and also demonstrated significant antioxidant activity in FRAP assay at 100μg/ml concentration. The crude extract did not show any antagonism against fungal pathogens Aspergillus niger and Aspergillus flavus. Crude extract demonstrated significant antibacterial activity against 5 studied pathogen, with the highest antagonism against Escherichia coli with a zone of inhibition of 22mm at 10mg/well concentration.</div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"2 1","pages":"Pages 1-8"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2018.09.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91759967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Biosurfactants—a new frontier for social and environmental safety: a mini review 生物表面活性剂——社会与环境安全的新前沿:综述

Biotechnology Research and Innovation Pub Date : 2018-01-01 DOI: 10.1016/j.biori.2018.09.001

Sweeta Akbari, Nour Hamid Abdurahman, Rosli Mohd Yunus, Fahim Fayaz, Oluwaseun Ruth Alara

{"title":"Biosurfactants—a new frontier for social and environmental safety: a mini review","authors":"Sweeta Akbari, Nour Hamid Abdurahman, Rosli Mohd Yunus, Fahim Fayaz, Oluwaseun Ruth Alara","doi":"10.1016/j.biori.2018.09.001","DOIUrl":"10.1016/j.biori.2018.09.001","url":null,"abstract":"<div>Biosurfactants are amphiphilic compounds synthesized from plants and microorganisms. These compounds are well known to be promising alternative molecules for industrial and domestic applications due to their high biodegradability, low toxicity, multi-functionality, environmental capability, and availability of resources. In recent times, the chemical compounds of biosurfactants have gained much attention because they are considered as a suitable alternative and eco-friendly materials for remediation technology. The current society is facing several challenges of implementation, enforcing environmental protection and climate change for the next generations. Thus, studies on human and environmental safety to improve the efficiency of sustainable technologies on environmental remediation are being investigated. Biosurfactants exhibited an efficient and successful application in various industries such as cosmetic, pharmaceutical, food, petroleum, agricultural, textile, and wastewater treatment. In this regard, this review focused on the advantages of biosurfactants over the synthetic surfactants produced from petroleum-based products along with their potential application in different industries.</div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"2 1","pages":"Pages 81-90"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2018.09.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85901755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 152

Mining and analysis of simple sequence repeats in the chloroplast genomes of genus Vigna 维纳属叶绿体基因组简单重复序列的挖掘与分析

Biotechnology Research and Innovation Pub Date : 2018-01-01 DOI: 10.1016/j.biori.2018.08.001

Nidhi Shukla , Himani Kuntal , Asheesh Shanker , Satyendra Nath Sharma

{"title":"Mining and analysis of simple sequence repeats in the chloroplast genomes of genus Vigna","authors":"Nidhi Shukla , Himani Kuntal , Asheesh Shanker , Satyendra Nath Sharma","doi":"10.1016/j.biori.2018.08.001","DOIUrl":"https://doi.org/10.1016/j.biori.2018.08.001","url":null,"abstract":"<div>The genus Vigna is distributed throughout the world and it is phylogenetically closely related to agriculturally important crops. Simple sequence repeats (SSRs) are defined as sequence repeat units that occur between 1 and 6bp and found in both coding and non-coding regions of genome. The availability of organelle genome sequence helps in understanding the organization of SSRs in them. In this study, chloroplast genome sequences of the genus Vigna were screened for the identification of chloroplast simple sequence repeats (cpSSRs). A total of 21, 24 and 25 cpSSRs in Vigna angularis, Vigna radiata and Vigna unguiculata, respectively, were mined computationally. The density of ∼1SSR/6.1–7.2kb was observed. Depending on the different repeat units, the length of SSRs ranged from 12 to 24 bp. Mononucleotides were the most abundant repeats followed by dinucleotide repeats. Hexanucleotide repeats were completely absent in the chloroplast genomes of genus Vigna. Seven cpSSRs were selected on the basis of repeats to study transferability. The transferable markers can be employed in genome mapping and comparative mapping among Vigna species in future studies.</div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"2 1","pages":"Pages 9-18"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2018.08.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91724749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Isolation, characterization and identification of extracellular enzyme producer Bacillus licheniformis from municipal wastewater and evaluation of their biodegradability 城市污水中胞外产酶物地衣芽孢杆菌的分离、鉴定及可降解性评价

Biotechnology Research and Innovation Pub Date : 2018-01-01 DOI: 10.1016/J.BIORI.2018.03.001

N. Sonune, A. Garode

引用次数: 24

Phytochemical constituents of Dracaena mahatma leaves and their anti-bacterial, anti-oxidant and anti-inflammatory significance 龙血树叶的植物化学成分及其抗菌、抗氧化和抗炎意义

Biotechnology Research and Innovation Pub Date : 2018-01-01 DOI: 10.1016/J.BIORI.2018.09.002

Saranya Shankar, Sugashini Settu, Gayathri Segaran, R. Sundar, L. Ravi

引用次数: 8

Biosynthesis of nanoparticles using eco-friendly factories and their role in plant pathogenicity: a review 生态工厂纳米颗粒生物合成及其在植物致病性中的作用综述

Biotechnology Research and Innovation Pub Date : 2018-01-01 DOI: 10.1016/j.biori.2018.09.003

Nayantara , Pawan Kaur

{"title":"Biosynthesis of nanoparticles using eco-friendly factories and their role in plant pathogenicity: a review","authors":"Nayantara , Pawan Kaur","doi":"10.1016/j.biori.2018.09.003","DOIUrl":"10.1016/j.biori.2018.09.003","url":null,"abstract":"<div>Nanoparticles (NPs) have been synthesized by various methods like physical, chemical and biological methods. Physical and chemical methods are costly and toxic to the environment. So there is an emerging need for production of nanoparticles using nontoxic, eco-friendly and reliable methods to expand their applications in agriculture field. Best option to achieve this goal is the use of biological entities such as microorganisms and plant extracts to synthesize nanoparticles. The main focus of this review is to compile the studies of synthesis of nanoparticles using “eco-friendly nano-factories” i.e., plant extract and microorganisms. Agriculture is an area where new technologies are often applied to improve the yield of crops. Plant diseases are one of the major factors that affect crop productivity. The problem with disease management lies with the detection of the exact stage of prevention. The employment of nanoparticles in agriculture field with some beneficial effects to the crops will be promising step toward nano-revolution in agriculture field. This review also summarizes antimicrobial activity of nanoparticles, their influence on the plant growth parameters and their role in plant pathogenicity.</div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"2 1","pages":"Pages 63-73"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2018.09.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89811603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 79

Preparation of silver nanoparticles by Garcinia mangostana stem extract and investigation of the antimicrobial properties 山竹藤茎提取物制备纳米银及其抗菌性能研究

Biotechnology Research and Innovation Pub Date : 2018-01-01 DOI: 10.1016/j.biori.2017.11.001

Perumal Karthiga

引用次数: 41