Biotechnology Research and Innovation最新文献

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Graduate programs in Brazil need reevaluation to contribute for innovation in biotechnology 巴西的研究生项目需要重新评估,以促进生物技术的创新
Biotechnology Research and Innovation Pub Date : 2017-01-01 DOI: 10.1016/j.biori.2017.01.001
Luiz Antonio Barreto de Castro
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引用次数: 0
Support engineering: relation between development of new supports for immobilization of lipases and their applications 支持工程:开发新的固定化脂肪酶的支持和他们的应用之间的关系
Biotechnology Research and Innovation Pub Date : 2017-01-01 DOI: 10.1016/j.biori.2017.01.004
Eliane Pereira Cipolatti , Evelin Andrade Manoel , Roberto Fernandez-Lafuente , Denise Maria Guimarães Freire
{"title":"Support engineering: relation between development of new supports for immobilization of lipases and their applications","authors":"Eliane Pereira Cipolatti ,&nbsp;Evelin Andrade Manoel ,&nbsp;Roberto Fernandez-Lafuente ,&nbsp;Denise Maria Guimarães Freire","doi":"10.1016/j.biori.2017.01.004","DOIUrl":"10.1016/j.biori.2017.01.004","url":null,"abstract":"<div><p>The growing interest in processes with the use of immobilized lipases guides to the development of new supports. In that way, the design and characterization of new supports for lipase immobilization have been increasingly popular in literature. Efforts to obtain “the perfect support” (a not accomplished yet) are described in this paper. Obviously, the choice and development of a support is directly related to the process in which it will be used, considering different factors as the media where the immobilzed enzyme will be used (whether aqueous, free or with solvents), potency of agitation, reactor configuration or substrates/products that will be involved. The present work discusses the use of some techniques of support synthesis in the case of core-shell particles, such as: miniemulsion, microemulsion, suspension, dispersion, the use of heterofunctional supports, whole-cell and processes of coimobilization. Some analytical tools for the investigation of enzyme immobilization are also presented, such as fourier transform infrared spectroscopy, as well as support characteristics that may be relevant for its final performance (e.g., specific surface area, particle diameter and particle size distribution and confocal laser scanning microscope).</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"1 1","pages":"Pages 26-34"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2017.01.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82897285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 32
Improved genetic transformation of Synechococcus elongatus PCC 7942 using linear DNA fragments in association with a DNase inhibitor 利用线性DNA片段与DNA酶抑制剂联合改良长聚球菌PCC 7942的遗传转化
Biotechnology Research and Innovation Pub Date : 2017-01-01 DOI: 10.1016/j.biori.2017.09.001
Daniela Volcan Almeida , Stefani Betina Boschmann Martens , Carlos Frederico Ceccon Lanes , Luis Fernando Marins
{"title":"Improved genetic transformation of Synechococcus elongatus PCC 7942 using linear DNA fragments in association with a DNase inhibitor","authors":"Daniela Volcan Almeida ,&nbsp;Stefani Betina Boschmann Martens ,&nbsp;Carlos Frederico Ceccon Lanes ,&nbsp;Luis Fernando Marins","doi":"10.1016/j.biori.2017.09.001","DOIUrl":"10.1016/j.biori.2017.09.001","url":null,"abstract":"<div><p>The genetic manipulation in many cyanobacterial strains is challenging yet. Thus, the development of new transformation protocols is desirable to facilitate the genetic engineering in cyanobacteria. Transformations using linear fragments yielded by PCR have advantages such as: less laborious methodology, faster procedure, low cost and unnecessary cloning steps. However, some strains presence extracellular nucleases, which reduce the efficiency in obtaining transformants. In this study, we demonstrate an improved protocol for genetic transformation in <em>Synechococcus elongatus</em> PCC 7942 using linear fragments employing EDTA-mediated inhibition of DNases. To conduct the transformation, linear PCR products containing the spectinomycin antibiotic resistance gene were employed. As result, 40<!--> <!-->mM EDTA treatment increased the number of transformants obtained by eightfold in comparison to the conventional protocol using plasmid DNA. Thus, the application of exonuclease inhibitors can be considered a relevant improvement to manipulate cyanobacteria in a more efficient, faster way and as a low-cost alternative. This protocol must be helpful for other strains of cyanobacteria.</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"1 1","pages":"Pages 123-128"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2017.09.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82052066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Structural diversity of carbohydrate esterases 碳水化合物酯酶的结构多样性
Biotechnology Research and Innovation Pub Date : 2017-01-01 DOI: 10.1016/j.biori.2017.02.001
Aline M. Nakamura, Alessandro S. Nascimento, Igor Polikarpov
{"title":"Structural diversity of carbohydrate esterases","authors":"Aline M. Nakamura,&nbsp;Alessandro S. Nascimento,&nbsp;Igor Polikarpov","doi":"10.1016/j.biori.2017.02.001","DOIUrl":"10.1016/j.biori.2017.02.001","url":null,"abstract":"<div><p>Carbohydrate esterases (CEs) catalyze the de-O or de-N-acylation by removing the ester decorations from carbohydrates. CEs are currently classified in 15 families in the Carbohydrate-Active Enzyme (CAZy) database, which classifies a large variety of enzymes that assemble, modify and breakdown carbohydrates and glycoconjugates. CEs have significant importance as biocatalysts in a variety of bioindustrial processes and applications. Thus, the understanding of molecular mechanisms involved in CE catalysis is essential. However, despite a rather large number of enzymes classified as CEs, just a few have been studied biochemically and only a handful has their three-dimensional structures determined and analyzed. Here, we present a brief overview of all currently classified CE families, mainly focusing on the structures and enzymatic activities of CEs.</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"1 1","pages":"Pages 35-51"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2017.02.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83925087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 90
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