Biotechnology Research and Innovation最新文献

{"title":"Biological activities of green coffee nanoemulsions evaluated through alternative methods: MTT, cellular proliferation, and HET-CAM assays","authors":"Elizandra Bruschi Buzanello, Susane Lopes Daniela Sousa Coelho Ana Paula Voyten, Simone Fanan, Letícia Mazzarino, Marcelo Maraschin","doi":"10.4322/biori.00112023","DOIUrl":"https://doi.org/10.4322/biori.00112023","url":null,"abstract":"Green coffee (Coffea arabica) has been widely explored by the cosmetic industry for its beneficial properties to the skin. The oil from coffee beans helps in hydration by retaining the lipids of the stratum corneum forming a barrier to the water in the skin, also preventing the aging. The green coffee paste also has positive implications in the development of cosmetic products with high biological activity, i.e., anti bacterial, antioxidant, proliferative, and aiding skin healing. This study aims to explore the synergistic effects of coffee constituents by investigating green coffee nanoemulsions. The evaluation will focus on their impact on cell proliferation, viability, as well as the potential risk of eye irritation. The findings will contribute to establishing the safety and efficacy criteria necessary for incorporating these nanoemulsions into cosmetic products. The nanoemulsions were approximately 200 nm in size, with monodispersive characteristics, and negative charged. In vitro biological tests showed that green coffee nanoemulsions had low cytotoxicity, stimulate cell proliferation and did not show eye irritation by the alternative methods to animal use that have been tested. The study demonstrated promising potential, but further research is necessary for its practical incorporation into the industry.","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135105367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiparasitic activity of lipid extracts from the subantarctic macroalgae Iridea cordata against Trichomonas vaginalis","authors":"Tallyson Nogueira Barbosaa, Mara Thais de Oliveira Silvaa, Ângela Sena-Lopesa, Frederico Schmitt Kremerb, Cláudio Martin Pereira de Pereirac, Fernanda Severo Sabedra Sousad, Fabiana Kommling Seixasd, Tiago Veiras Collaresd, Sibele Borsuka","doi":"10.4322/biori.00102023","DOIUrl":"https://doi.org/10.4322/biori.00102023","url":null,"abstract":"In this study, we demonstrate the promising antiparasitic activity of natural extracts as an alternative treatment for trichomoniasis. We evaluated the in vitro and in silico antiparasitic activity of Iridea cordata extracts, obtained in two distinct development phases: Iridea cordata tetrasporaphyte phase (IFT) and Iridea cordata cystocarp phase (IFC). To determine the minimum inhibitory concentration (MIC) and 50% inhibitory concentration (IC50), we tested five concentrations of the extracts against Trichomonas vaginalis (ATCC 30236). To gain insights into the mechanisms underlying the antiparasitic activity and possible adverse effects, the extracts were subjected to cytotoxicity assays in VERO and human vaginal epithelial (HVMII) cells, gene expression analyses, and their components’ interactions with T. vaginalis proteins were analyzed through molecular docking. In the in vitro biological assay, IFT and IFC exhibited a MIC of 600 μg/mL while showing an IC50 of 150 μg/mL and 300 μg/mL and inhibiting 80% and 97% of T. vaginalis trophozoites, respectively. Importantly, no cytotoxic effects were observed on VERO and HMVII cells for IFC and IFT at 600 μg/mL, indicating their safety. IFC and IFT induced significant differences in gene expression compared to the negative control, DMSO, and metronidazole, suggesting their potential modulation of T. vaginalis genes. Moreover, in silico analysis revealed that constituents of both extracts interacted, with significant free-binding energy, with proteins that are important for T. vaginalis survival. Overall, this study provides evidence of the antiparasitic activity of Iridea cordata extracts against T. vaginalis and supports further evaluation of its extracts as a promising treatment for trichomoniasis.","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135009223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Survival mechanisms of microorganisms occurring in acid mine drainage: sulfur, iron, carbon, and nitrogen metabolic pathways","authors":"Estácio Jussie Odisi, Diego Serrasol do Amaral, Marcus Adonai Castro da Silva, André Oliveira de Souza Lima, Leonardo Rubi Rörig","doi":"10.4322/biori.00092023","DOIUrl":"https://doi.org/10.4322/biori.00092023","url":null,"abstract":"Despite its economic importance, mining usually generates intense environmental degradation. The excavation process carried out in mining activities exposes minerals to atmospheric oxygen and water, conditioning a series of biogeochemical processes that can lead to the production of acid mine drainage (AMD). AMD has low pH and high concentrations of sulphates and heavy metals, creating environments with extreme conditions for life. These environments are usually inhabited by microorganisms able to acquire energy from iron and sulfur, using limited sources of carbon and nitrogen. In addition, these microorganisms need mechanisms to resist to extremely low pH and high concentration of heavy metals that can be toxic and lethal to the cellular structure. Acid stress tolerance involves active mechanisms to maintain intracellular pH at adequate levels despite low external values, and adaptive processes against acid stress allowing microorganisms to operate metabolically at low pH. The set of these adaptations give microorganisms the possibility of surviving in AMD environments and, consequently, represent potential for bioremediation and other biotechnological applications like biomining and search for biomolecules for industrial processes. The purpose of this review was to compile the metabolic and adaptive mechanisms involved in the survival of microorganisms occurring in AMD environments, focusing on how they utilize sulfur, iron, carbon and nitrogen metabolic pathways.","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"145 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135053528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real-Time PCR Assay for detection and quantification of Leishmania: standardization, positive control, validation, and intra-laboratory assay","authors":"Manuel Hospinal-Santiani, Carlos Ricardo Soccol, Susan Grace Karp, Eduardo Scopel Ferreira da Costa, Luiz Alberto Junior Letti, Germana Davila dos Santos, Vanete Thomaz Soccol","doi":"10.4322/biori.00062022","DOIUrl":"https://doi.org/10.4322/biori.00062022","url":null,"abstract":"His study aimed to develop a method to investigate PCR sensitivity for diagnosis and ensure reproducibility for parasite load quantification in tissues based on qPCR. In the first step, genes were selected to quantify the parasite load; then, a standard was developed to quantify the concentration of different Leishmania species. These tools were evaluated in intra-laboratory assays. The sensitivity was determined as 0.01 parasites/μL, and the method was reproducible with 100% concordance among human participants in the intra-laboratory validation study. Furthermore, the results demonstrated the specificity of the method in detecting the genus Leishmania without showing cross-reaction with Trypanosoma cruzi or human DNA.","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135053516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence of fumonisins and strategies for biocontrol in beer production: a systematic review","authors":"Adriane Buczynski, Juliana Vitória Messias Bittencourt, Mariana Machado Fidelis do Nascimento, Elisabete Hiromi Hashimoto","doi":"10.4322/biori.00032023","DOIUrl":"https://doi.org/10.4322/biori.00032023","url":null,"abstract":"Beer is one of the most consumed alcoholic beverages in the world and is basically made up of water, barley malt, hops and yeast other components that also be added in production such as adjuncts. Corn is among the most used adjuncts and considered a viable and affordable grain to partially replace barley. However, there is a constant concern about the occurrence of mycotoxins and subsequent contamination in beer processing. Corn can be contaminated by a type of mycotoxin called fumonisins, produced by the fungus Fusarium verticillioides. The relationship between the detection of fumonisins and the use of corn-based adjuncts in beer processing has been described for over 25 years. However, the occurrence and effect on beer processing are less reported in the literature when compared to scientific publications on the relationship between the fungus F. graminearum and its mycotoxins. Given this scenario, the objective of this revision was to develop a Methodi Ordinatio systematic literature review on three subjects: fumonisins occurrence in beer, the contamination effect by F. verticillioides and fumonisins on beer processing and viable biocontrol methods to improve this problem. In total, 22 articles on the occurrence of fumonisins in beer were selected, which showed that countries on the African continent are the ones with the highest levels of mycotoxins contamination. In addition, 17 papers were selected to discuss the effect of contamination by F. verticillioides and fumonisins on beer processing. Together, these works verified the presence of fumonisins in the raw material and in the final product after processing, demonstrating that more measures are needed to restrict the development of fumonisin-producing fungi. Finally, 21 papers were selected on viable biocontrol methods to improve beer processing. Specifically, it has been described that conventional food processing methods are not able to eliminate fumonisins, and biological control methods are more effective as they reduce or eliminate them. Such methods involve physicochemical processes such as adsorption and enzymatic biodegradation.","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135151190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosynthesis of fatty acids and biosurfactants by the yeast Yarrowia lipolytica with emphasis on metabolic networks and bioinformatics","authors":"C. Bauer, C. Schmitz, M. Landell, M. Maraschin","doi":"10.4322/biori.202201","DOIUrl":"https://doi.org/10.4322/biori.202201","url":null,"abstract":": Yarrowia lipolytica is a haploid, aerobic, and non-pathogenic yeast with biotechnological importance due to its lipolytic and proteolytic properties. It is capable of producing lipases, biosurfactants, long chain fatty acids, and also metabolizing different types of carbon sources such as hydrocarbons. Besides, Y. lipolytica also receives attention due to its ability to produce organic acids. In this review, we explore its metabolic abilities to produce fatty acids for the fine chemical industry (fatty acid-derived bio-based compounds) and also biosurfactants, important for the food and pharmaceutical industries and bioremediation. With the accelerated advance of ‘omics’ technologies, e.g., genomics, proteomics, metabolomics, genetic and metabolic engineering, combined with bioinformatics, substantial data are increasingly available, allowing to optimize biotechnological applications of this microorganism. Thus, this review covers the","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78824186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of light/dark cycles on the growth of Haematococcus pluvialis in mixotrophic\u0000 cultivation with alternative culture media","authors":"L. H. Sipaúba-Tavares, M. G. Tedesque, D. C. Fenerick, R. N. Millan, B. Scardoeli-Truzzi","doi":"10.4322/biori.20226202","DOIUrl":"https://doi.org/10.4322/biori.20226202","url":null,"abstract":"","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72854293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A systematic review of drying methods and their impact on technological characteristics of sourdough type III","authors":"I. D. Marco, Cássia Maria da Silva, J. O. Moraes, L. A. A. Menezes, Marília Miotto, J. Laurindo, J. Lindner","doi":"10.4322/biori.202203","DOIUrl":"https://doi.org/10.4322/biori.202203","url":null,"abstract":"2452-0721/2452-0721 © 2022 sociedade Brasileira de Biotecnologia. this is an Open access article distributed under the terms of the creative commons attribution-Noncommercial No Derivative license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium provided the original work is properly cited and the work is not changed in any A systematic review of drying methods and their impact on technological characteristics of sourdough type III","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89714420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peptides and current methods on bovine tuberculosis diagnosis","authors":"Violetta Dias Pacce, N. R. Oliveira, Jean Michel Dela Vedova-Costa, O. Dellagostin, V. Thomaz-Soccol","doi":"10.4322/biori.202202","DOIUrl":"https://doi.org/10.4322/biori.202202","url":null,"abstract":"2452-0721/2452-0721 © 2022 Sociedade Brasileira de Biotecnologia. This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial No Derivative License, which permits unrestricted noncommercial use, distribution, and reproduction in any medium provided the original work is properly cited and the work is not changed in any Peptides and current methods on bovine tuberculosis diagnosis","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"81 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77297485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro determination of the sun protection factor (SPF) and photochemoprotection\u0000 potential of aqueous extract of banana peels (Musa sp.) against UV-B radiation","authors":"Susane Lopes, Giulia Galani, L. A. Peruch, M. Maraschin","doi":"10.4322/biori.20226203","DOIUrl":"https://doi.org/10.4322/biori.20226203","url":null,"abstract":": Banana ( Musa sp.) fruits have been investigated by the scientific community for their healing properties due to their composition in bioactive compounds. In this study, the aqueous extract (AE) of banana peels ( Musa sp., cv . Prata Anã) was chemically characterized and investigated regarding its antioxidant and photochemoprotective potential against UV-B radiation in BALB/3T3 clone A31 mouse fibroblasts. The results showed the AE as a source of phenolic compounds, mainly gallic acid. Antioxidant activity of AE was demonstrated by its ability to scavenge DPPH free radicals and to reduce ferric–tripyridyltriazine complex. A sun protection factor (SPF) of up to 36.4 was determined through the Mansur equation. The AE revealed low cytotoxicity (96 ± 12% cell viability - 100 µg/mL) to mouse fibroblasts and the determination of the photochemoprotective effect revealed preservation of collagen biosynthesis in cells exposed to UV-B (38.6 ± 7%), also contributing to the maintenance of cellular and nuclear morphologies. Thus, the AE of banana peels seems to be an interesting candidate for pharmaceutical/cosmetic formulations to combat skin’s photoaging caused by UV-B radiation.","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85305831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}