Biotechnology Research and Innovation最新文献

{"title":"Identification of three robust and efficient Saccharomyces cerevisiae strains isolated from Brazilian's cachaça distilleries","authors":"Raquel de Sousa Paredes, I. P. V. Vieira, Vinicius Mattos de Mello, L. Vilela, R. Schwan, E. Eleutherio","doi":"10.1016/J.BIORI.2018.07.001","DOIUrl":"https://doi.org/10.1016/J.BIORI.2018.07.001","url":null,"abstract":"","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"17 1","pages":"22-29"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84138539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized biodegradation of carcinogenic fungicide Carbendazim by Bacillus licheniformis JTC-3 from agro-effluent","authors":"J. Panda, Tiyasha Kanjilal, S. Das","doi":"10.1016/J.BIORI.2017.10.004","DOIUrl":"https://doi.org/10.1016/J.BIORI.2017.10.004","url":null,"abstract":"","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"18 1","pages":"45-57"},"PeriodicalIF":0.0,"publicationDate":"2017-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91354969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of silver nanoparticles by Garcinia mangostana stem extract and investigation of the antimicrobial properties","authors":"P. Karthiga","doi":"10.1016/J.BIORI.2017.11.001","DOIUrl":"https://doi.org/10.1016/J.BIORI.2017.11.001","url":null,"abstract":"","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"128 1","pages":"30-36"},"PeriodicalIF":0.0,"publicationDate":"2017-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76106899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent developments and innovations in solid state fermentation","authors":"Carlos Ricardo Soccol,&nbsp;Eduardo Scopel Ferreira da Costa,&nbsp;Luiz Alberto Junior Letti,&nbsp;Susan Grace Karp,&nbsp;Adenise Lorenci Woiciechowski,&nbsp;Luciana Porto de Souza Vandenberghe","doi":"10.1016/j.biori.2017.01.002","DOIUrl":"10.1016/j.biori.2017.01.002","url":null,"abstract":"<div><p>After forty years of research development, an overview of solid-state fermentation (SSF), focusing on its applications, mainly of the very recent papers of the last five years, is presented. This review comprises the most important developed processes concerning the production of enzymes, biopulping processes, and traditional processes, for food fermentation, such as the production of Chinese <em>daqu</em> and <em>koji</em>, and industrial important biomolecules such as organic acids, pigments, phenolic compounds, aromas and biosorbents. SSF bioreactors design that has been developed is reported, so as the solutions for the classical drawbacks and the most important cases of successful employment of the technique are described. And, finally, it is summarized a very interesting report of patents and innovations regarding SSF products and processes.</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"1 1","pages":"Pages 52-71"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2017.01.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75771783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knocking down chitin synthase 2 by RNAi is lethal to the cotton boll weevil","authors":"L.L.P. Macedo ,&nbsp;J.D. Antonino de Souza Junior ,&nbsp;R.R. Coelho ,&nbsp;F.C.A. Fonseca ,&nbsp;A.A.P. Firmino ,&nbsp;M.C.M. Silva ,&nbsp;R.R. Fragoso ,&nbsp;E.V.S. Albuquerque ,&nbsp;M.S. Silva ,&nbsp;J. de Almeida Engler ,&nbsp;W.R. Terra ,&nbsp;M.F. Grossi-de-Sa","doi":"10.1016/j.biori.2017.04.001","DOIUrl":"10.1016/j.biori.2017.04.001","url":null,"abstract":"<div><p>The cotton boll weevil (<em>Anthonomus grandis</em>) is the most destructive cotton insect pest in Brazil. The endophytic habit of this insect makes difficult its chemical control. Chitin synthase (CHS) is an integral membrane glycosyltransferase that is essential for chitin chain polymerization and deposition in insect chitinous structures, such as the peritrophic membrane (PM). Because it is not present in plants or vertebrates, CHS can be considered a promising target for eco-friendly biotechnological approaches, such as RNA interference (RNAi)-mediated gene silencing. Considering the relevance of <em>CHS</em> genes in the chitin biosynthetic pathway in insects, we report here the molecular cloning of the full-length <em>CHS2</em> cDNA from the cotton boll weevil, and its functional validation <em>via</em> RNAi. The <em>AgraCHS2</em> cDNA sequence is 4,869<!--> <!-->bp, with a 4,446<!--> <!-->bp open reading frame that encodes a predicted protein with 1,482 amino acid residues. Predicted protein has high similarity (53 to 78%) with other insects CHS. Moreover, only one copy is present in <em>A. grandis</em> genome. Transcriptional analysis showed that <em>AgraCHS2</em> transcripts are restricted to the insect midgut at the third-larval instar and adult stages, which are considered the main feeding stages. RNAi-mediated knockdown of the <em>AgraCHS2</em> affected <em>A. grandis</em> development, resulting in oviposition reduction of 93% and leading to 100% adult mortality. These data, in addition to the observation of PM severe disorganization in the midgut after <em>AgraCHS2</em> knock-down, suggest <em>AgraCHS2</em> as a promising target for developing RNAi-based biotechnological alternatives to specifically control the cotton boll weevil.</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"1 1","pages":"Pages 72-86"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2017.04.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89713820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacophore modeling and 3D QSAR studies for prediction of matrix metalloproteinases inhibitory activity of hydroxamate derivatives","authors":"Dharmender Rathee ,&nbsp;Viney Lather ,&nbsp;Harish Dureja","doi":"10.1016/j.biori.2017.10.002","DOIUrl":"10.1016/j.biori.2017.10.002","url":null,"abstract":"<div><p>In order to develop potent inhibitors of matrix metalloproteinase (MMP-2 and MMP-9) as anticancer agents, pharmacophore modeling and three-dimensional quantitative structure–activity relationship (3D-QSAR) models were established using PHASE 3.0. A pharmacophore 5-point (AAARR) model was developed for the studied dataset and the generated model was used to derive the predictive atom-based 3D-QSAR models. After identifying a valid hypothesis, we developed 3D-QSAR models applying the PLS algorithm. The selected 3D-QSAR models were suggestive of the vitality of the electron-withdrawing feature for the MMPs inhibitory potential. In addition, hydrophobic groups, hydrogen bond donor groups, positive ionic and negative ionic features also positively contributed to the MMPs inhibitory potential along with the electron-withdrawing feature. The developed models were statistically robust (MMP-2 <em>Q</em>² <!-->=<!--> <!-->0.51; pred <em>R</em>² <!-->=<!--> <!-->0.67; MMP-9 <em>Q</em>² <!-->=<!--> <!-->0.59; pred <em>R</em>² <!-->=<!--> <!-->0.77). The QSAR results help in identifying a relationship between structural features of hydroxamate derivatives and their activities which could be useful to design newer MMP inhibitors.</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"1 1","pages":"Pages 112-122"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2017.10.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73976491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The development of veterinary vaccines: a review of traditional methods and modern biotechnology approaches","authors":"Sérgio Jorge,&nbsp;Odir Antônio Dellagostin","doi":"10.1016/j.biori.2017.10.001","DOIUrl":"10.1016/j.biori.2017.10.001","url":null,"abstract":"<div><p>The immunization of animals has been carried out for centuries and is generally accepted as the most cost-effective and sustainable method of controlling infectious veterinary diseases. Up to twenty years ago, most veterinary vaccines were either inactivated organisms that were formulated with an oil-based adjuvant or live attenuated vaccines. In many cases, these formulations were not very effective. The discovery of antigen/gene delivery systems has facilitated the development of novel prophylactic and therapeutic veterinary vaccines. To identify vaccine candidates in genomic sequences, a revolutionary approach was established that stems from the assumption that antibodies are more readily able to access surface and secreted than cytoplasm proteins; as such, they represent ideal vaccine candidates. The approach, which is known as reverse vaccinology, uses several bioinformatics algorithms to predict antigen localization and it has been successfully applied to immunize against many veterinary diseases. This review examines some of the main topics that have emerged in the veterinary vaccine field with the use of modern biotechnology techniques.</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"1 1","pages":"Pages 6-13"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2017.10.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77274638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanisms of biomass increase in plants","authors":"Marcelo de Freitas Lima ,&nbsp;Nubia Barbosa Eloy ,&nbsp;João Antonio Batista de Siqueira ,&nbsp;Dirk Inzé ,&nbsp;Adriana Silva Hemerly ,&nbsp;Paulo Cavalcanti Gomes Ferreira","doi":"10.1016/j.biori.2017.08.001","DOIUrl":"10.1016/j.biori.2017.08.001","url":null,"abstract":"<div><p>Biomass consumption continues to increase worldwide for the provision of human energy needs. These high pressures for energy will determine the demand for crop plants as a resource for biofuel, heat and electricity. Thus, the search for plant traits associated with genetic increases in yield is unconditional. Here, we propose exploiting recent advances in plant biomass enhancement in non-crop as well as in crop plants. For this purpose, biotechnological approaches that are well known rapid ways of enhancing the plant traits, as well as the traditional way of improving plants through plant breeding selecting for desirable phenotypes are excellent techniques to improve plant biomass and reduce the dependence on fossil fuels. Obviously, many genes can be associated with promising phenotypes however this review will focus on genes selected from different plant networks.</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"1 1","pages":"Pages 14-25"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2017.08.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84321041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alkaline phosphatase activity of a phosphate solubilizing Alcaligenes faecalis, isolated from Mangrove soil","authors":"Bikash Chandra Behera ,&nbsp;Himendra Yadav ,&nbsp;Santosh Kumar Singh ,&nbsp;Bijay Kumar Sethi ,&nbsp;Rashmi Ranjan Mishra ,&nbsp;Sony Kumari ,&nbsp;Hrudayanath Thatoi","doi":"10.1016/j.biori.2017.01.003","DOIUrl":"10.1016/j.biori.2017.01.003","url":null,"abstract":"<div><p>Microorganisms are capable of converting insoluble phosphate into a bioavailable form through solubilization and mineralization processes. Hence in the present study a phosphate solubilizing bacterium, PSB-26, was isolated from mangrove of the Mahanadi delta using NBRIP-agar and NBRIP-BPB broth containing tricalcium phosphate as the phosphate source. Based on phenotypic and molecular characterization, the strain was identified as <em>Alcaligenes faecalis</em>. The maximum phosphate solubilizing activity of the strain was found to be 48<!--> <!-->μg/ml with decrease in pH of the growth medium from 7.0 to 3.2. During phosphate solubilization, various organic acids, such as oxalic acid (289<!--> <!-->mg/L), citric acid (0.2<!--> <!-->mg/L), malic acid (0.3<!--> <!-->mg/L), succinic acid (0.5<!--> <!-->mg/L) and acetic acid (0.4<!--> <!-->mg/L) produced in the broth culture were detected through HPLC analysis. Crude alkaline phosphatase activity of the strain was determined by <em>p</em>-nitrophenyl phosphate assay and optimized with different growth parameters to obtain maximum enzyme production. Under optimized sets of conditions, maximum alkaline phosphatase activity of 93.7<!--> <!-->U/ml was observed. Partially purified alkaline phosphatase exhibited three protein bands of sizes approximately 45<!--> <!-->kDa, 25<!--> <!-->kDa and 17<!--> <!-->kDa. Partially purified alkaline phosphatase during characterization showed maximum activity at pH 9.0 (96.53<!--> <!-->U/ml), temperature of 45<!--> <!-->°C (97.99<!--> <!-->U/ml) and substrate concentration of 1.75<!--> <!-->mg/ml (96.51<!--> <!-->U/ml). The effect of the bacterium on growth of <em>Arabidopsis thaliana</em> plant showed that inoculation of bacterial culture exhibited better growth in comparison to the control. Hence the phosphate solubilizing and alkaline phosphatase production activity of the bacterium may have probable use for future biotechnological application.</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"1 1","pages":"Pages 101-111"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2017.01.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76500389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prospection and identification of nematotoxic compounds from Canavalia ensiformis seeds effective in the control of the root knot nematode Meloidogyne incognita","authors":"Thales L. Rocha ,&nbsp;Carla Becker Soll ,&nbsp;Berin A. Boughton ,&nbsp;Tiago S. Silva ,&nbsp;Klaus Oldach ,&nbsp;Alexandre A.P. Firmino ,&nbsp;Damien L. Callahan ,&nbsp;John Sheedy ,&nbsp;Edilberto R. Silveira ,&nbsp;Regina M.D.G. Carneiro ,&nbsp;Luciano P. Silva ,&nbsp;Vera L.P. Polez ,&nbsp;Patrícia B. Pelegrini ,&nbsp;Antony Bacic ,&nbsp;Maria F. Grossi-de-Sa ,&nbsp;Ute Roessner","doi":"10.1016/j.biori.2017.10.003","DOIUrl":"10.1016/j.biori.2017.10.003","url":null,"abstract":"<div><p><em>Meloidogyne incognita</em> is responsible for significant crop losses worldwide. The main strategy to control this phytopathogen is still based on synthetic nematicides that are harmful to human health and the environment. In this context, aqueous seed extracts of antagonistic plants were screened for molecules effective toward the infective stage (J₂) of <em>M. incognita</em>. The aqueous crude extract of <em>Canavalia ensiformis</em> (ACE<em>Ce</em>) showed the highest nematicidal activity (87<!--> <!-->±<!--> <!-->3% mortality). ACE<em>Ce</em> dialysis fractionation allowed the collection of an external dialysate (ED<em>Ce</em>) containing molecules smaller than 3.5<!--> <!-->kDa effective against J₂ (96<!--> <!-->±<!--> <!-->3.0% mortality); innocuous toward non targeted organisms as saprophytic nematodes, fungi, bacterium and insects larvae; active against J2 (96<!--> <!-->±<!--> <!-->2% mortality) after heating (50<!--> <!-->°C); no cytotoxic for bovine red blood cells; reduction of <em>M. incognita</em> eggs masses by 82.5% in tomato plants at green house conditions. Fractionation of the ED<em>Ce</em> by reversed-phase high-performance liquid chromatography (RP-HPLC) separated five nematotoxic fractions. Analyses of those fractions based on gas chromatography–mass spectrometry (GC–MS), liquid chromatography–mass spectrometry (LCMS) and nuclear magnetic resonance (NMR) identified nine specific metabolites, follow-up testing of the individual authentic standards of each metabolite as the compounds: <span>d</span>-glucose, <span>l</span>-canavanine, xanthotoxin, <em>cis</em>-aconitic acid, <em>trans</em>-aconitic acid, malic acid, citric acid, palmitic acid and S-carboxymethylcysteine; all them confirmed to possess nematotoxic properties. However, some of those metabolites were not described previously demonstrating biological action against <em>M. incognita.</em></p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"1 1","pages":"Pages 87-100"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2017.10.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78174922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}