{"title":"热带假丝酵母能够以蔗渣半纤维素水解物为碳源生产酵母单细胞蛋白","authors":"Cristina Emanuela Barbosa Magalhães , Manoel Santiago Souza-Neto , Spartaco Astolfi-Filho , Italo Thiago Silveira Rocha Matos","doi":"10.1016/j.biori.2018.08.002","DOIUrl":null,"url":null,"abstract":"<div><p>The world demand for foods will be the most important bottle-neck for human being maintenance. In this context, the research for alternative proteins sources aiming human and animal feeding is a necessary approach. A wild-type strain of <em>Candida tropicalis</em> (KP276650) was identified as potential yeast single cell protein (YSCP) producer using sugarcane bagasse hemicellulosic hydrolysate (SBHH) as substrate. Were evaluated the cell growth and sugar consumption along fermentation assay. Biomass yield (Y<sub>B</sub>, g<!--> <!-->g<sup>−1</sup>), protein percentage (%), protein yield (Y<sub>P</sub>, g<!--> <!-->g<sup>−1</sup>) and yeast biomass productivity (YBP, g<!--> <!-->L<sup>−1</sup> <!-->h<sup>−1</sup>) were calculated. Fermentation assay reach a total of 96<!--> <!-->h, being the total reducing sugar present in the media (59.94<!--> <!-->g<!--> <!-->L<sup>−1</sup>) fully consumed. Final biomass produced was 16.97<!--> <!-->g<!--> <!-->L<sup>−1</sup>, and the YBP was 0.1767<!--> <!-->g<!--> <!-->L<sup>−1</sup> <!-->h<sup>−1</sup>. Percentage of protein in yeast biomass produced was 60.05%, biomass yield (Y<sub>B</sub>) and protein yield (Y<sub>P</sub>) were, respectively, 0.28 and 0.17<!--> <!-->g<!--> <!-->g<sup>−1</sup>. The Y<sub>B</sub> and Y<sub>P</sub> obtained are close to the observed for some industrial strains of yeasts. The percentage of protein is greater than the expected for most of yeasts and fungi. These results indicate that the using of <em>C. tropicalis</em> (KP276650) for YSCP production may to aggregate high value for sugarcane bagasse.</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"2 1","pages":"Pages 19-21"},"PeriodicalIF":0.0000,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2018.08.002","citationCount":"17","resultStr":"{\"title\":\"Candida tropicalis able to produce yeast single cell protein using sugarcane bagasse hemicellulosic hydrolysate as carbon source\",\"authors\":\"Cristina Emanuela Barbosa Magalhães , Manoel Santiago Souza-Neto , Spartaco Astolfi-Filho , Italo Thiago Silveira Rocha Matos\",\"doi\":\"10.1016/j.biori.2018.08.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The world demand for foods will be the most important bottle-neck for human being maintenance. In this context, the research for alternative proteins sources aiming human and animal feeding is a necessary approach. A wild-type strain of <em>Candida tropicalis</em> (KP276650) was identified as potential yeast single cell protein (YSCP) producer using sugarcane bagasse hemicellulosic hydrolysate (SBHH) as substrate. Were evaluated the cell growth and sugar consumption along fermentation assay. Biomass yield (Y<sub>B</sub>, g<!--> <!-->g<sup>−1</sup>), protein percentage (%), protein yield (Y<sub>P</sub>, g<!--> <!-->g<sup>−1</sup>) and yeast biomass productivity (YBP, g<!--> <!-->L<sup>−1</sup> <!-->h<sup>−1</sup>) were calculated. Fermentation assay reach a total of 96<!--> <!-->h, being the total reducing sugar present in the media (59.94<!--> <!-->g<!--> <!-->L<sup>−1</sup>) fully consumed. Final biomass produced was 16.97<!--> <!-->g<!--> <!-->L<sup>−1</sup>, and the YBP was 0.1767<!--> <!-->g<!--> <!-->L<sup>−1</sup> <!-->h<sup>−1</sup>. Percentage of protein in yeast biomass produced was 60.05%, biomass yield (Y<sub>B</sub>) and protein yield (Y<sub>P</sub>) were, respectively, 0.28 and 0.17<!--> <!-->g<!--> <!-->g<sup>−1</sup>. The Y<sub>B</sub> and Y<sub>P</sub> obtained are close to the observed for some industrial strains of yeasts. The percentage of protein is greater than the expected for most of yeasts and fungi. These results indicate that the using of <em>C. tropicalis</em> (KP276650) for YSCP production may to aggregate high value for sugarcane bagasse.</p></div>\",\"PeriodicalId\":100187,\"journal\":{\"name\":\"Biotechnology Research and Innovation\",\"volume\":\"2 1\",\"pages\":\"Pages 19-21\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.biori.2018.08.002\",\"citationCount\":\"17\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biotechnology Research and Innovation\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2452072118300479\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnology Research and Innovation","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2452072118300479","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 17
摘要
世界粮食需求将是人类生存的最大瓶颈。在此背景下,针对人类和动物饲料的替代蛋白质来源的研究是必要的途径。以甘蔗甘蔗渣半纤维素水解物(shbhh)为底物,鉴定出一株热带念珠菌(KP276650)为酵母单细胞蛋白(YSCP)的潜在生产菌株。通过发酵试验对细胞生长和糖消耗进行了评价。计算生物量产量(YB, g g−1)、蛋白质百分比(%)、蛋白质产量(YP, g g−1)和酵母生物量生产力(YBP, g L−1 h−1)。发酵试验达到96小时,培养基中存在的总还原糖(59.94 g L−1)完全消耗。最终生物量为16.97 g L−1,YBP为0.1767 g L−1 h−1。酵母生物量中蛋白质的比例为60.05%,生物量产量(YB)和蛋白质产量(YP)分别为0.28和0.17 g g−1。所得的YB和YP与某些工业酵母菌的观测值接近。对于大多数酵母和真菌,蛋白质的百分比比预期的要高。这些结果表明,利用热带镰刀菌(KP276650)生产YSCP可能会为甘蔗渣积累高价值。
Candida tropicalis able to produce yeast single cell protein using sugarcane bagasse hemicellulosic hydrolysate as carbon source
The world demand for foods will be the most important bottle-neck for human being maintenance. In this context, the research for alternative proteins sources aiming human and animal feeding is a necessary approach. A wild-type strain of Candida tropicalis (KP276650) was identified as potential yeast single cell protein (YSCP) producer using sugarcane bagasse hemicellulosic hydrolysate (SBHH) as substrate. Were evaluated the cell growth and sugar consumption along fermentation assay. Biomass yield (YB, g g−1), protein percentage (%), protein yield (YP, g g−1) and yeast biomass productivity (YBP, g L−1 h−1) were calculated. Fermentation assay reach a total of 96 h, being the total reducing sugar present in the media (59.94 g L−1) fully consumed. Final biomass produced was 16.97 g L−1, and the YBP was 0.1767 g L−1 h−1. Percentage of protein in yeast biomass produced was 60.05%, biomass yield (YB) and protein yield (YP) were, respectively, 0.28 and 0.17 g g−1. The YB and YP obtained are close to the observed for some industrial strains of yeasts. The percentage of protein is greater than the expected for most of yeasts and fungi. These results indicate that the using of C. tropicalis (KP276650) for YSCP production may to aggregate high value for sugarcane bagasse.