Biotechnology Research and Innovation最新文献

{"title":"Brazilian red propolis extracts: study of chemical composition by ESI-MS/MS (ESI+) and cytotoxic profiles against colon cancer cell lines","authors":"Denis Amilton dos Santos ,&nbsp;Fernanda Mosena Munari ,&nbsp;Caroline Olivieri da Silva Frozza ,&nbsp;Sidnei Moura ,&nbsp;Thiago Barcellos ,&nbsp;João Antonio Pêgas Henriques ,&nbsp;Mariana Roesch-Ely","doi":"10.1016/j.biori.2019.02.001","DOIUrl":"10.1016/j.biori.2019.02.001","url":null,"abstract":"<div><p>Red propolis is a natural resin mixture produced by honeybees and presents a source of active compounds with a variety of biological activities. In this study, we describe the chemical characterization and potential antitumor activity of total extract of Brazilian red propolis and its fractions. Fractions were obtained through column chromatography revealing 14 different compounds in all samples, which were determined and distinguished of other isobar molecules by fragmentation pathways by ESI-MS/MS in positive mode. Some molecules as cis-asarone or trans-isoelemicin were identified and distinguish from elemicin compound and vestitol or isovestitol were also distinguished from neovestitol by fragmention pathway. Other important compounds as liquiritigenin was differentiated from isoliquiritigenin and formononetin from dalbergin.</p><p>MTT viability assay showed different toxicity in cell lines after exposition to total extract and fractions. Fractions 05 and 06 had more selectivity against HT-29 and HCT-116 cancer cells, respectively, in relation to normal cells. IC₅₀ (ranging of 72.45<!--> <!-->±<!--> <!-->6.57 to 73.58<!--> <!-->±<!--> <!-->1.00<!--> <!-->μg/mL) in cancer cells were lower than reported in total extracts of propolis. May-Grunwald/Giemsa staining revealed cellular morphological changes after exposition to higher concentrations of red propolis extracts. Fractionation techniques can contribute to reduce chemical diversity verified in propolis mixtures, generating fractions with improved biological activity and contributing to the development of new strategies for discovery of natural compounds against cancer.</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"3 1","pages":"Pages 120-130"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2019.02.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85591912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the role of chromium reductase for Cr (VI) reduction by Pseudomonas species isolated from Cr (VI) contaminated effluent","authors":"Parvaze Ahmad Wani ,&nbsp;Shazia Wahid ,&nbsp;Mohd Sajjad Ahmad Khan ,&nbsp;Nusrat Rafi ,&nbsp;Nazia Wahid","doi":"10.1016/j.biori.2019.04.001","DOIUrl":"https://doi.org/10.1016/j.biori.2019.04.001","url":null,"abstract":"<div><p>This study observed the role of pH, chromium (VI) concentrations, temperatures and chromium reductases for of Cr (VI) reduction. Bacteria isolated from effluent were identified as <em>Pseudomonas sp.</em> by molecular analysis. Bacterial strain MAI4 showed significant reduction at pH 7 (84%), 100<!--> <!-->μg<!--> <!-->Cr (VI)/ml (86%) and 35<!--> <!-->°C (86%). Increase in time of incubation increased Cr (VI) reduction by <em>P. entomophila</em> MAI4 significantly and 120<!--> <!-->h of incubation showed maximum reduction of Cr (VI). <em>P. entomophila</em> MAI4 also showed significant reduction of Cr (VI) (80%) in industrial waste water. Bacterial strain MAI4 reduced Cr (VI) into Cr (III) after 120<!--> <!-->h which was detected as 70<!--> <!-->±<!--> <!-->3<!--> <!-->μg/ml in cell pellet and 30<!--> <!-->±<!--> <!-->2<!--> <!-->μg/ml in supernatant, respectively. Chromium reductase found in cell free extracts (CFE) reduced almost all Cr (VI) to Cr (III) compared to cell debris. Based on reduction under <em>in vitro</em> and <em>in vivo</em> conditions, <em>Pseudomonas sp.</em> MAI4 could be used as a bioremediator of Cr (VI) in contaminated effluents.</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"3 1","pages":"Pages 38-46"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2019.04.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91763728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stability and tissue-specific Cry10Aa overexpression improves cotton resistance to the cotton boll weevil","authors":"Thuanne Pires Ribeiro ,&nbsp;Marcos Fernando Basso ,&nbsp;Mayara Holanda de Carvalho ,&nbsp;Leonardo Lima Pepino de Macedo ,&nbsp;Dagna Maria Laurindo da Silva ,&nbsp;Isabela Tristan Lourenço-Tessutti ,&nbsp;Osmundo Brilhante de Oliveira-Neto ,&nbsp;Eduardo Romano de Campos-Pinto ,&nbsp;Wagner Alexandre Lucena ,&nbsp;Maria Cristina Mattar da Silva ,&nbsp;Bruna Mendes Diniz Tripode ,&nbsp;Tatianne Piza Ferrari Abreu-Jardim ,&nbsp;José Ednilson Miranda ,&nbsp;Marcio Alves-Ferreira ,&nbsp;Carolina Vianna Morgante ,&nbsp;Maria Fatima Grossi-de-Sa","doi":"10.1016/j.biori.2019.12.003","DOIUrl":"10.1016/j.biori.2019.12.003","url":null,"abstract":"<div><p>The cotton boll weevil (CBW, <em>Anthonomus grandis</em>) is the most destructive cotton insect pest affecting cotton crops. To overcome this problem, CBW-resistant genetically modified cotton plants overexpressing <em>Bacillus thuringiensis</em> entomotoxins were successfully obtained. Previous results showed that the overexpression of Cry10Aa protoxin led to high mortality of the CBW larvae in greenhouse conditions. In this study, we advanced three more generations (T2 to T4), with several cotton events constitutively overexpressing the Cry10Aa protoxin, and the transgene stability and agronomic performance were investigated. In addition, stable transgenic cotton overexpressing the Cry10Aa active (Cry10Aa protoxin lacking the α-helix N-terminal) driven by cotton flower bud-specific promoters were generated and characterized. Cotton events constitutively or tissue-specifically overexpressing the Cry10Aa protein (protoxin or active) represented mortality percentages of the CBW larva of up to 85 % in plants under greenhouse conditions. Events overexpressing the Cry10Aa active under control of the flower bud-specific promoter showed higher protein accumulation in stamens and carpels compared to the events with constitutive expression. Our findings suggested that the high stability of the Cry10Aa transgene and the elevated expression level and protein accumulation in flower bud tissues (primarily in stamen and carpels) contribute to improved resistance to CBW larvae. Finally, some notable events were selected with potential for future field trials in different cotton-producing regions of Brazil. Therefore, cotton events overexpressing high levels of the Cry10Aa protein in flower bud tissue may have a strong potential for commercial use in the integrated management of CBW.</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"3 ","pages":"Pages 27-41"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2019.12.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84195668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endogenous soybean peptide overexpression: an alternative to protect plants against root-knot nematodes","authors":"Ciliana Rechenmacher ,&nbsp;Beatriz Wiebke-Strohm ,&nbsp;Luisa Abruzzi de Oliveira-Busatto ,&nbsp;Ricardo Luis Mayer Weber ,&nbsp;Mariana Cristina Moraes Corso ,&nbsp;Valéria Stefania Lopes-Caitar ,&nbsp;Suellen Mika Hishinuma Silva ,&nbsp;Waldir Pereira Dias ,&nbsp;Francismar Correa Marcelino-Guimarães ,&nbsp;Celia Regina Carlini ,&nbsp;Maria Helena Bodanese-Zanettini","doi":"10.1016/j.biori.2019.12.002","DOIUrl":"10.1016/j.biori.2019.12.002","url":null,"abstract":"<div><p>Nematodes are pathogens of many important crops, including soybean. The main species found in Brazil are root-knot (<em>Meloidogyne</em> spp.), cyst (<em>Heterodera glycines</em>), root lesion (<em>Pratylenchus brachyurus</em>) and reniform (<em>Rotylenculus reniformis</em>) nematodes. Ureases are traditionally known for catalyzing the hydrolysis of urea to ammonia and carbon dioxide. Besides the main function, they present other independent biological roles, including toxic activities against insects, specially Coleoptera and Hemiptera, and fungi. In previous work, the DNA sequence encoding an insecticidal peptide - named Jaburetox - was identified in a <em>Canavalia ensiformes</em> urease gene. The recombinant Jaburetox exhibited toxicity against insects. Subsequently, the DNA sequence corresponding to Jaburetox was identified as part of the soybean Eu4 urease gene, with the resulting peptide named Soyuretox. In the present study, explants of soybean were transformed with <em>Agrobacterium rhizogenes</em> and ‘composite’ plants produced consisting of wild-type shoots and transgenic hairy roots overexpressing Soyuretox. Thereafter soybean plants overexpressing Soyuretox were obtained through bombardment transformation. Due to similarity between nematode and insect digestion mechanisms, we challenged composite and whole-transgenic plants with the nematode <em>Meloidogyne javanica</em>. Hairy roots overexpressing Soyuretox exhibited a significant reduction (48 %; p<!--> <!-->&lt;<!--> <!-->0.05) in the average reproductive factor when compared with empty-vector transformed hairy roots. Transgenic plants overexpressing Soyuretox also exhibited significant reduction (37.5 %; p<!--> <!-->&lt;<!--> <!-->0.05) in reproductive factor when compared with non-transformed plants. This study demonstrates the potential of Soyuretox in confering resistance against nematodes, representing a new alternative control method for nematodes in economically important crops.</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"3 ","pages":"Pages 10-18"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2019.12.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79134818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Yin and Yang in plant breeding: the trade-off between plant growth yield and tolerance to stresses","authors":"Aline Cunha da Silva ,&nbsp;Marcelo de Freitas Lima ,&nbsp;Nubia Barbosa Eloy ,&nbsp;Flávia Thiebaut ,&nbsp;Patrícia Montessoro ,&nbsp;Adriana Silva Hemerly ,&nbsp;Paulo Cavalcanti Gomes Ferreira","doi":"10.1016/j.biori.2020.02.001","DOIUrl":"10.1016/j.biori.2020.02.001","url":null,"abstract":"<div><p>Plants have the ability to recognize and respond to biotic and abiotic stresses that are responsible for considerable yield losses in agriculture. Currently, a central goal of crop deployment is to develop the capacity to be tolerant to multiple stresses without a reduction in fitness. Still, many efforts to release such plants have failed because, frequently, there is a trade-off between growth and tolerance to stresses. Conventional breeding plays an essential role in crop improvement, but it is necessary to develop new tools, using for instance CRISPR, to produce new cultivars exhibiting tolerance to stress without significant yield penalty. In this short review we discuss novel strategies that can be employed to produce novel cultivars that would increase plant productivity without being hindered by potential negative effects of the immune response on plant development.</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"3 ","pages":"Pages 73-79"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2020.02.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76620954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relevant aspects of the biosynthesis of porous aluminas using glycosides and carbohydrates as biological templates","authors":"Ángela B. Sifontes ,&nbsp;Edward Ávila ,&nbsp;Brenda Gutiérrez ,&nbsp;Mine Rengifo ,&nbsp;Andrea Mónaco ,&nbsp;Yraida Díaz ,&nbsp;Ligia Llovera","doi":"10.1016/j.biori.2019.01.004","DOIUrl":"10.1016/j.biori.2019.01.004","url":null,"abstract":"<div><p>This research paper comparison of the use of biological templates obtained from steviol glycosides and glucose (monosaccharide) directed toward the synthesis of metal oxides. The results obtained shown the synthesis of aluminum oxides in an aqueous medium, using different green porogenic agents. The influences of the aging period and its impact on the alumina's porosity and phase transition were evaluated. The FTIR studies provided evidence of the surface modification of the aluminum oxide by carboxylate groups generated in the hydrolysis of diterpenic glycosides. The application of prolonged aging periods favored the production of η-alumina vs γ-alumina in the synthesis in which <em>Stevia rebaudiana</em> was used. The materials were characterized, using XRD, TGA, N₂ physical adsorption, FE-SEM, NMR, FTIR and TPD-NH₃. The TGA profiles indicate appreciable differences as to the yields achieved between samples prepared with the two biological templates (alumina–stevia up to 62%, alumina–glucose yields of 30%). The acidity obtained for the different aluminas synthesized by the use of biological templates showed a trend toward: glucose<!--> <!-->&gt;<!--> <!-->stevia<!--> <!-->&gt;<!--> <!-->sol–gel method/stevia, in the range of 0.994–0.485<!--> <!-->mmol/g.</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"3 1","pages":"Pages 22-37"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2019.01.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76503187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesenchymal stem cells from human adipose tissue and bone repair: a literature review","authors":"Vivian Alonso-Goulart ,&nbsp;Lorraine Braga Ferreira ,&nbsp;Cristiane Angélico Duarte ,&nbsp;Isabela Lemos de Lima ,&nbsp;Enza Rafaela Ferreira ,&nbsp;Bárbara Candido de Oliveira ,&nbsp;Luna Nascimento Vargas ,&nbsp;Dayane Dotto de Moraes ,&nbsp;Isaura Beatriz Borges Silva ,&nbsp;Rafael de Oliveira Faria ,&nbsp;Aline Gomes de Souza ,&nbsp;Leticia de Souza Castro-Filice","doi":"10.1016/j.biori.2017.10.005","DOIUrl":"10.1016/j.biori.2017.10.005","url":null,"abstract":"<div><p>Mesenchymal stem cells (MSCs) emerge as potential tools for treatment of various diseases. Isolation methods and tissue of origin are important factors that determine the amount of obtained cells and their ability to differentiate. MSCs can be isolated from adipose tissue (ADSCs), bone marrow (BMSCs) or umbilical cord (UC-MSCs), and its characterization must follow the criteria required by the International Society for Cellular Therapy. Osteogenic differentiation capacity of ADSCs can still vary according to the culture medium used, as well as by adding factors that can alter signaling pathways and enhance bone differentiation. In addition, nanotechnology has also been used to increase osteoblastic induction and differentiation. ADSCs enhanced the prospect of treatment in different diseases, and in regenerative medicine, these cells can also be associated with different biomaterials. There is a great progress in studies with ADSCs, mainly because it is easy to access, which makes bioengineering techniques for bone tissue feasible.</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"2 1","pages":"Pages 74-80"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2017.10.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76850054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized biodegradation of carcinogenic fungicide Carbendazim by Bacillus licheniformis JTC-3 from agro-effluent","authors":"Jigisha Panda,&nbsp;Tiyasha Kanjilal,&nbsp;Sumona Das","doi":"10.1016/j.biori.2017.10.004","DOIUrl":"https://doi.org/10.1016/j.biori.2017.10.004","url":null,"abstract":"<div><p>The aim of this research work is to study biodegradation of Carbendazim, a globally used carcinogenic fungicide, by novel bacterial strain <em>Bacillus licheniformis</em> JTC-3, isolated from local agro-effluent, as it wrecks havoc on human immune, nervous and endocrine systems, upon consumption. The strain was characterized by various biochemical tests, antibiotic assay and identified by 16S rDNA analysis. High Performance Liquid Chromatographic studies showed its biodegradation capacity to be very high (73.2% of initial Carbendazim concentration), in Minimal Salt Media, within 24<!--> <!-->h of incubation. Various growth parameters (temperature, agitation speed, pH, substrate concentration) were optimized by Central Composite Design to get the biodegradation rate of 0.305<!--> <!-->mg/L of Carbendazim/h/mg biomass. Scanning Electron Micrograph, X-ray diffraction, Fourier Transform Infra-Red spectroscopic microanalysis and toxicity testing of metabolic end-product confirmed formation of non-toxic, crystalline 2-hydroxybenzimidazole. Accordingly, a plausible mechanism of biodegradation of Carbendazim has been proposed here. The isolate's growth curve and the rate kinetics mathematically fitted well with Gompertz model and second order reaction, respectively.</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"2 1","pages":"Pages 45-57"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2017.10.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91725616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First characterization report of natural pearl of Pinctada fucata from Gulf of Mannar","authors":"C.P. Suja,&nbsp;S. Lakshmana Senthil,&nbsp;Bridget Jeyatha,&nbsp;Jensi Ponmalar,&nbsp;Koncies Mary","doi":"10.1016/j.biori.2017.11.002","DOIUrl":"10.1016/j.biori.2017.11.002","url":null,"abstract":"<div><p>The present study is aimed to characterize the natural pearl of <em>Pinctada fucata</em> from Gulf of Mannar by Scanning Electron Microscope (SEM) and Energy Dispersive Studies (EDS). Pearl oysters (<em>P. fucata</em>) from Kayalpattinam, Gulf of Mannar, were landed as a by-catch in the bottom set gill net at a depth of 4<!--> <!-->m and collected for tissue culture studies. During mantle tissue dissection, a good lustrous, round pearl of 1.5<!--> <!-->mm size was found in the mantle fold of pearl oyster <em>P. fucata</em>. This evidenced the existence of natural pearl oyster beds and natural pearls in this region. It was analyzed by Scanning Electron Microscopy (SEM) and Energy Dispersive Spectroscopy (EDS) to find out the composition of nacre. Parallel orientation of crystals to form the lamellar formation of nacre is clearly visible in SEM. Pseudo-hexagonal aragonite crystals arranged in a uniform layer and joined together to form a lamella with inter-lamellar matrix. Two forms of calcium (CaO and CaCO₃) obtained in EDS analysis. Calcium content in the natural pearl is 66.05% which is clearly reveals the aragonite form. Niobium (Nb) was attained at 6% in natural pearl which is the first report in its kind and it may also play a role in the formation of lustrous layer. There are so many characterization reports available about mother of pearl in shell of different species, but this is the first report of natural pearl from <em>P. fucata</em>.</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"2 1","pages":"Pages 58-62"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2017.11.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85340033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of three robust and efficient Saccharomyces cerevisiae strains isolated from Brazilian's cachaça distilleries","authors":"Raquel de Sousa Paredes ,&nbsp;Igor Patrick Vasconcelos Vieira ,&nbsp;Vinícius Mattos de Mello ,&nbsp;Leonardo de Figueiredo Vilela ,&nbsp;Rosane Freitas Schwan ,&nbsp;Elis Cristina Araújo Eleutherio","doi":"10.1016/j.biori.2018.07.001","DOIUrl":"https://doi.org/10.1016/j.biori.2018.07.001","url":null,"abstract":"<div><p>Three isolated strains from Brazilian's cachaça distilleries were tested for glucose consumption and ethanol production during the fermentation process. The ethanol productivity, cell viability and mitochondrial mutagenic rate of each strain was evaluated at the end of a 24<!--> <!-->h-fermentation round. The strains’ resistance to the fermentation process was evaluated after cell recycling followed by another round of fermentation. Among the isolated industrial strains evaluated, the strains CCA083 and CA751 has shown the best performance in terms of productivity and yield. The cachaça strain CCA083 was able to keep a high glucose consumption and ethanol productivity during the second fermentation round. This result suggests that the stress response mechanism is activated due to the heat shock pretreatment, which creates a protective effect on the cell. Therefore, these results could bring up to light a new framework for industries and researchers in order to develop strains with increased stress tolerance on first and second-generation ethanol production.</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"2 1","pages":"Pages 22-29"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2018.07.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91724748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}