Investigation of the role of chromium reductase for Cr (VI) reduction by Pseudomonas species isolated from Cr (VI) contaminated effluent

Biotechnology Research and Innovation Pub Date : 2019-01-01 DOI:10.1016/j.biori.2019.04.001

Parvaze Ahmad Wani , Shazia Wahid , Mohd Sajjad Ahmad Khan , Nusrat Rafi , Nazia Wahid

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引用次数: 37

Abstract

This study observed the role of pH, chromium (VI) concentrations, temperatures and chromium reductases for of Cr (VI) reduction. Bacteria isolated from effluent were identified as Pseudomonas sp. by molecular analysis. Bacterial strain MAI4 showed significant reduction at pH 7 (84%), 100 μg Cr (VI)/ml (86%) and 35 °C (86%). Increase in time of incubation increased Cr (VI) reduction by P. entomophila MAI4 significantly and 120 h of incubation showed maximum reduction of Cr (VI). P. entomophila MAI4 also showed significant reduction of Cr (VI) (80%) in industrial waste water. Bacterial strain MAI4 reduced Cr (VI) into Cr (III) after 120 h which was detected as 70 ± 3 μg/ml in cell pellet and 30 ± 2 μg/ml in supernatant, respectively. Chromium reductase found in cell free extracts (CFE) reduced almost all Cr (VI) to Cr (III) compared to cell debris. Based on reduction under in vitro and in vivo conditions, Pseudomonas sp. MAI4 could be used as a bioremediator of Cr (VI) in contaminated effluents.

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铬还原酶在Cr (VI)污染废水中假单胞菌还原Cr (VI)中的作用研究

本研究观察了pH、铬(VI)浓度、温度和铬还原酶对Cr (VI)还原的作用。从废水中分离出的细菌经分子分析鉴定为假单胞菌。菌株MAI4在pH为7(84%)、100 μg Cr (VI)/ml(86%)和35°C(86%)时均表现出显著的还原作用。随着孵育时间的延长，嗜虫线虫MAI4对Cr (VI)的还原效果显著增强，孵育120 h时对Cr (VI)的还原效果最大，对工业废水中Cr (VI)的还原效果也显著(80%)。菌株MAI4在120 h后将Cr (VI)还原为Cr (III)，细胞颗粒中Cr (VI)含量为70±3 μg/ml，上清中Cr (III)含量为30±2 μg/ml。与细胞碎片相比，在无细胞提取物(CFE)中发现的铬还原酶几乎将所有的铬(VI)还原为铬(III)。基于体外和体内条件下的还原，假单胞菌sp. MAI4可作为污染废水中Cr (VI)的生物修复介质。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biotechnology Research and Innovation

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