Biotechnology Research and Innovation最新文献

{"title":"Brain signal evaluation of children with Autism Spectrum Disorder in the interaction with a social robot","authors":"Christiane Goulart ,&nbsp;Carlos Valadão ,&nbsp;Eliete Caldeira ,&nbsp;Teodiano Bastos","doi":"10.1016/j.biori.2018.11.003","DOIUrl":"https://doi.org/10.1016/j.biori.2018.11.003","url":null,"abstract":"<div><p>This work consists of a pilot study in which brain signals captured by electroencephalography (EEG) of children diagnosed with Autism Spectrum Disorder (ASD) are evaluated during the interaction with a social robot. Social skills and interaction of children with ASD with the robot are proposed and assessed, using quantitative scale and images recorded by video cameras. During interaction with the robot, results show high activation of alpha and beta rhythms in brain regions important to social skills. Quantitative scales indicate a positive children–robot interaction and point out the social robot as a potential tool to stimulate social skills and facilitate the interaction with other people.</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"3 1","pages":"Pages 60-68"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2018.11.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91763731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential distribution of polyphenols in plants using multivariate techniques","authors":"Vinod Kumar, Anket Sharma, Sukhmeen Kaur Kohli, Shagun Bali, Manik Sharma, Rakesh Kumar, R. Bhardwaj, Ashwani Kumar Thukral","doi":"10.1016/J.BIORI.2019.03.001","DOIUrl":"https://doi.org/10.1016/J.BIORI.2019.03.001","url":null,"abstract":"","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75312327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential distribution of polyphenols in plants using multivariate techniques","authors":"Vinod Kumar ,&nbsp;Anket Sharma ,&nbsp;Sukhmeen Kaur Kohli ,&nbsp;Shagun Bali ,&nbsp;Manik Sharma ,&nbsp;Rakesh Kumar ,&nbsp;Renu Bhardwaj ,&nbsp;Ashwani Kumar Thukral","doi":"10.1016/j.biori.2019.03.001","DOIUrl":"https://doi.org/10.1016/j.biori.2019.03.001","url":null,"abstract":"<div><p>Plants form a substantive portion of human diet that contains a plethora of structurally diverse polyphenols. These polyphenols extend both nutritional as well as disease preventive benefits to the consumer. Many ancient medicinal systems like Ayurveda reiterate the consumption of particular polyphenol rich plant in specific medical condition. Therefore, it is necessary to create a database of the contents of different polyphenols in various plants. This study compiles a bibliographic review of polyphenol distribution in different plants along with their statistical analysis like Pearson's correlation matrix, cluster analysis, principal component and factor analysis, and non-metric multidimensional scaling. It was observed that caffeic acid and quercetin were the most abundant polyphenols in the plants. A significant positive correlation of umbelliferone was found with kaempferol and epicatechin. Species having close proximities on the basis of polyphenols were <em>Parthenium hysterophorus</em>, <em>Rumex dentatus</em>, <em>Achyranthus aspera</em>, <em>Chenopodium ambrosoides</em>, <em>Cannabis sativa</em>, <em>Rhododendron arboreum</em>, <em>Alternanthera philoxeroides</em>, <em>Debregeasia longifolia</em> and <em>C. album</em>. Factor analysis showed four underlying factors for polyphenols. Factor-1 had maximum loadings on epicatechin, umbelliferone and kaempferol. Gallic acid and catechin had maximum loadings on factor-2. Factor-3 had maximum loadings on chlorogenic acid and quercetin, and factor-4 had maximum loading on coumaric acid.</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"3 1","pages":"Pages 1-21"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2019.03.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91763727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative approach reveals new insights into photosynthetic and redox protection in ex vitro tobacco plantlets acclimatization to increasing light intensity","authors":"Cinthya F. Vieira ,&nbsp;Fabricio E.L. Carvalho ,&nbsp;Yugo Lima-Melo ,&nbsp;Cristina P.S. Carvalho ,&nbsp;Milton C. Lima Neto ,&nbsp;Marcio O. Martins ,&nbsp;Joaquim A.G. Silveira","doi":"10.1016/j.biori.2020.04.001","DOIUrl":"10.1016/j.biori.2020.04.001","url":null,"abstract":"<div><p>Integrative mechanisms involving photosynthetic and antioxidant protection regulated by light intensity during <em>in vitro</em> and <em>ex vitro</em> plantlets acclimatization are poorly understood. Tobacco plantlets grown under <em>in vitro</em> and <em>ex vitro</em> environments were exposed to different light regimes to evaluate the role of photosynthesis and antioxidant protection. <em>In vitro</em> plantlets displayed a narrow photosynthetic capacity to cope with light as revealed by low net CO₂ assimilation (<em>P</em>_N), decreased actual quantum efficiency of photosystem II (ΦPSII) associated with non-induction of non-photochemical quenching (NPQ). In contrast, acclimated <em>ex vitro</em> plants showed strong stimulation in <em>P</em>_N whereas ΦPSII and NPQ remained at high levels. <em>In vitro</em> plantlets exposed to moderate light suffered strong oxidative stress associated with increased activities of superoxide dismutases, catalases and ascorbate peroxidases, revealing an ineffective antioxidant system. In contrast, <em>ex vitro</em> plants presented lower oxidative damage in parallel to unchanged enzymatic activities, indicating an efficient antioxidant steady state. The levels of reduced ascorbate (ASC) and glutathione (GSH) were also increased only in <em>ex vitro</em> plants in response to excess light. An integrative study based on correlation networks and principal component analyses (PCA) corroborate that the two plant groups indeed displayed contrasting acclimation processes. In conclusion, during <em>in vitro</em> to <em>ex vitro</em> transition, tobacco plantlets exposed to increasing light display physiological adjustments involving photosynthesis and improvement of the enzymatic and non-enzymatic antioxidant systems. These findings highlight the importance of integrative approaches to understand <em>ex vitr</em>o acclimatization to environmental stimuli.</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"3 ","pages":"Pages 59-72"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2020.04.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81842824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Absorption of polycyclic aromatic hydrocarbons onto depolymerized lignocellulosic wastes by Streptomyces viridosporus T7A","authors":"Ana Caroline de Oliveira ,&nbsp;Ana Aguilar-Galvez ,&nbsp;David Campos ,&nbsp;Hervé Rogez","doi":"10.1016/j.biori.2019.04.002","DOIUrl":"10.1016/j.biori.2019.04.002","url":null,"abstract":"<div><p>Preparations containing mainly lignified cell walls are effective adsorbents of hydrophobic carcinogens <em>in vitro</em>. Therefore, this innovative study aimed to structurally modify lignocellulosic materials in an enzymatic and environmentally friendly manner to improve the efficiency of sequestering three polycyclic aromatic hydrocarbons (PAHs) by adsorption. Submerged fermentations were performed to produce lignin peroxidase (LiP) in medium supplemented with three different lignocellulosic wastes (0.5% (w/v); açai seed, sugarcane bagasse, and seed coat of Brazil nut), followed by posterior hydrolysis of these three residues and subsequent adsorption to generate hydrolysate wastes. Among the three wastes, the açai seed was the most favourable carbon source for LiP production because a high enzyme activity peak was quickly achieved. Sugarcane bagasse residue was most readily hydrolysed (82.44%), and it had the highest increase in adsorption of the three PAHs tested dissolved in olive oil, from 15.67% of benzo[a]pyrene adsorbed before treatment to 39.45% after treatment. The depolymerisation of wastes may have increased binding sites for olive oil favouring the adsorption of PAHs on hydrolysed residues.</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"3 1","pages":"Pages 131-143"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2019.04.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88136836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Avicennia marina extracts on Labeo rohita (Ham) challenged with Pseudomonas fluorescens","authors":"G. Dinesh Kumar ,&nbsp;M. Karthik ,&nbsp;R. Rajakumar ,&nbsp;K. Baskar","doi":"10.1016/j.biori.2018.10.002","DOIUrl":"https://doi.org/10.1016/j.biori.2018.10.002","url":null,"abstract":"<div><p>The medicinal plant <em>Avicennia marina</em> was evaluated for their immunostimulatory activity on <em>Pseudomonas fluorescens</em> infested fish, <em>Labeo rohita</em>. The fish was dosed intraperitoneally at 10, 20 and 30<!--> <!-->ppm concentrations of ethanolic leaves extract of <em>A. marina</em> and control. After 10, 20 and 30 days of treatments, the immunological, hematological and serum protein level of fish was assessed in control and treatments. All the concentration of plant leaves extract significantly enhanced the agglutination, hematological parameters and total serum protein on 30th days after treatment. The highest agglutination activity was observed in the group treated with 30<!--> <!-->ppm concentration of <em>A. marina</em> on 20 days. The WBC, RBC and hemoglobin content was increased with increasing concentration of the treatments. The results, clearly indicates that <em>A. marina</em> leaves extract will be used as immunostimulatory agent to aquaculture for mass production of healthy fish.</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"3 1","pages":"Pages 54-59"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2018.10.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90127741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial fuel cells potential of marine actinobacteria Actinoalloteichus sp. MHA15 from the Havelock island of the Andamans, India","authors":"Rajagopal Gobalakrishnan ,&nbsp;Rameshbabu Bhuvaneswari","doi":"10.1016/j.biori.2019.01.003","DOIUrl":"10.1016/j.biori.2019.01.003","url":null,"abstract":"<div><p>In the recent past, use of fossil fuel is on the rise and has triggered global energy crisis. So, renewable bioenergy is viewed as one of the means to tackle this problem. In this context, interest in microbial fuel cells (MFCs) is increasing and gaining popularity due to their ability to convert the organic wastes into renewable energy. Present investigation is on the bioelectricity production by a marine actinobacterium isolated from the Havelock island of the Andamans. Actinobacterial colonies were isolated from the sediment samples, using Kuster's agar. 19 morphologically distinct strains were subjected to cellulase enzyme screening. Among them, higher cellulose degradation capacity was found in the strain MHA15. This potential strain was selected and identified as a species close to <em>Actinoalloteichus cyanogriseus</em>. The strain was subjected to bioelectricity generation using sugarcane bagasse as substrate and was evaluated in a dual chambered microbial fuel cell. In the MFC, initial voltage output started at 160<!--> <!-->mV and it gradually increased, reaching a maximum of 257<!--> <!-->mV at the 3rd hour in the actinobacterial incubated bagasse solution. From the MFC analyzed sludge bagasse, microbial diversity was searched using different media and only actinobacterial colonies were observed. Conventional taxonomic characters of the isolates were identical to the potential actinobacterial strain MHA15 which produced bioelectricity from bagasse. Marine actinobacteria, with their unique nature, differ very much in many aspects from their terrestrial counterparts and are known to produce diverse spectra of novel and useful substances and excellent bioactivity. Results of the present study have ascertained that the marine actinobacterial strain <em>Actinoalloteichus</em> sp. MHA15 is capable of generating bioelectricity and there is much scope for utilizing such marine actinobacteria for large scale production of bioelectricity, after further in-depth studies.</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"3 1","pages":"Pages 144-158"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2019.01.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88540291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Avicennia marina extracts on Labeo rohita (Ham) challenged with Pseudomonas fluorescens","authors":"G. D. Kumar, M. Karthik, R. Rajakumar, K. Baskar","doi":"10.1016/J.BIORI.2018.10.002","DOIUrl":"https://doi.org/10.1016/J.BIORI.2018.10.002","url":null,"abstract":"","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77745762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response with TH1 profile obtained in vaccine formulation against Caseous Lymphadenitis in animal model C57 Black/6","authors":"Daniela Droppa-Almeida ,&nbsp;Wanessa L.P. Vivas ,&nbsp;Ricardo Evangelista Fraga ,&nbsp;Andrea F.S. Rezende ,&nbsp;Lumar Lucena Alves ,&nbsp;Roberto Meyer ,&nbsp;Isabel B. Lima-Verde ,&nbsp;Odir Delagostin ,&nbsp;Sibele Borsuk ,&nbsp;Francine F. Padilha","doi":"10.1016/j.biori.2018.11.002","DOIUrl":"10.1016/j.biori.2018.11.002","url":null,"abstract":"<div><p>Caseous Lymphadenitis (CLA) is a contagious disease that compromises the quality of life of sheep and goats. Caused by <em>Corynebacterium pseudotuberculosis</em> CLA is responsible for considerable economic losses in sheep and goat farming. Therefore, the search for preventive measures, such as the development of vaccines is increasing. To evaluate the immunoprotective response of experimental vaccines different murine models susceptible to infections are used. Thus, the objective of this study was to evaluate the protective potential of the recombinant subunit vaccine using an endoglycosidase (rCP40) of <em>C. pseudotuberculosis</em> associated with Saponin and Complete Freund adjuvant (CFA) in murine model C57/Black6. Thus, four groups of animals were separated, where G1 and G2 were control groups and G3 and G4 were experimental groups (rCP40<!--> <!-->+<!--> <!-->Saponin) and (rCP40<!--> <!-->+<!--> <!-->CFA) respectively. The evaluation of the production of reactive antibodies to rCP40 showed that the animals inoculated with the adjuvants presented potentiation of the cellular and humoral immune response, presenting higher production of IgG2a and IgG2b. After the challenge, only the control groups died, while in the experimental groups, although some survived, they presented granulomas, which are characteristics of CLA.</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"3 1","pages":"Pages 192-196"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2018.11.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84001621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Industrial production, patent landscape, and market trends of arachidonic acid-rich oil of Mortierella alpina","authors":"Luis Daniel Goyzueta Mamani ,&nbsp;Antonio Irineudo Magalhães Jr. ,&nbsp;Zhenhua Ruan ,&nbsp;Júlio Cesar de Carvalho ,&nbsp;Carlos Ricardo Soccol","doi":"10.1016/j.biori.2019.02.002","DOIUrl":"10.1016/j.biori.2019.02.002","url":null,"abstract":"<div><p>Polyunsaturated fatty acids are essential, health-promoting nutrients that are widely used in medicine, pharmaceutics, cosmetics, nutrition, and other fields. Suitable plant and animal sources for these lipids are limited, but alternatives have been actively researched and developed in the last three decades, especially those produced microbiologically. Arachidonic acid (ARA) is one of the most valuable among nutraceutical lipids, being associated with the good development of the nervous central system and enhancement of immune response. Currently, microbial sources of ARA are used for industrial production due to their rapid and controllable production, as compared to animal (fish) and plant sources. Microbial sources are also eco-friendly and reduce the pressure on marine life. The fungus <em>Mortierella alpina</em> is one of the most important microbial lipid sources, but there are few accounts of industrial and market information regarding this microorganism.</p><p>At the current pace, the industrial production of ARA is projected to reach 410 thousand tons by 2025, which will not be enough to meet the demand. This will keep stimulating research for intensified production, which can be optimized in terms of concentration and yields (through bioprospection and metabolic and biomolecular engineering), productivity and economics (through media optimization and byproduct use), and formulation. This work reviews aspects such as the important considerations for industrial production of arachidonic acid-rich oil by <em>Mortierella alpina</em>, based on patents and studies, and presents a global market analysis and forecast.</p></div>","PeriodicalId":100187,"journal":{"name":"Biotechnology Research and Innovation","volume":"3 1","pages":"Pages 103-119"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.biori.2019.02.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83416431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}