Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects最新文献

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Biosynthesis of a new uric acid ribonucleotide 一种新的尿酸核糖核苷酸的生物合成
Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-09-11 Epub Date: 2003-02-05 DOI: 10.1016/0926-6550(64)90182-3
Dolph Hatfield, Roy A. Greenland, H.L. Stewart, James B. Wyngaarden
{"title":"Biosynthesis of a new uric acid ribonucleotide","authors":"Dolph Hatfield, Roy A. Greenland, H.L. Stewart, James B. Wyngaarden","doi":"10.1016/0926-6550(64)90182-3","DOIUrl":"10.1016/0926-6550(64)90182-3","url":null,"abstract":"","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 1","pages":"Pages 163-166"},"PeriodicalIF":0.0,"publicationDate":"1964-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90182-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23779723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Ribonucleotide incorporation by a soluble enzyme from maize 由玉米可溶性酶合成的核糖核苷酸
Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-09-11 Epub Date: 2003-02-05 DOI: 10.1016/0926-6550(64)90190-2
Rusty J. Mansf , G.David Novelli
{"title":"Ribonucleotide incorporation by a soluble enzyme from maize","authors":"Rusty J. Mansf , G.David Novelli","doi":"10.1016/0926-6550(64)90190-2","DOIUrl":"10.1016/0926-6550(64)90190-2","url":null,"abstract":"","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 1","pages":"Pages 186-188"},"PeriodicalIF":0.0,"publicationDate":"1964-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90190-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23779731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
A deoxyribonucleic acid-protein complex having DNA-polymerase and RNA-polymerase activities in cell-free extracts of Escherichia coli 在大肠杆菌的无细胞提取物中具有dna聚合酶和rna聚合酶活性的脱氧核糖核酸-蛋白质复合物
Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-09-11 Epub Date: 2003-02-05 DOI: 10.1016/0926-6550(64)90168-9
Motoko Kadoya, Hiromi Mitsui , Yasuyuki Takagi , Eiko Otaka , Hideho Suzuki , Syozo Osawa
{"title":"A deoxyribonucleic acid-protein complex having DNA-polymerase and RNA-polymerase activities in cell-free extracts of Escherichia coli","authors":"Motoko Kadoya,&nbsp;Hiromi Mitsui ,&nbsp;Yasuyuki Takagi ,&nbsp;Eiko Otaka ,&nbsp;Hideho Suzuki ,&nbsp;Syozo Osawa","doi":"10.1016/0926-6550(64)90168-9","DOIUrl":"10.1016/0926-6550(64)90168-9","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. A deoxyribonucleoprotein, found in extracts of <em>Escherichia coli</em> B(H), has been investigated with respect to its role in DNA and RNA synthesis.</p></span></li><li><span>2.</span><span><p>2. A deoxyribonucleoprotein in ribosome-free cell extract could be separated from the other cellular components either with gel filtration or zone electrophoresis.</p></span></li><li><span>3.</span><span><p>3. The isolated deoxyribonucleoprotein was composed of roughly 80% of DNA a and 20% of protein.</p></span></li><li><span>4.</span><span><p>4. Association of DNA polymerase (deoxynucleosidetriphosphate: DNA deoxynucleotidyltransferase, EC 2.7.7.7) and RNA polymerase (nucleosidetriphosphate: RNA nucleotidyltransferase (DNA dependent), EC 2.7.7.6) with this deoxyribonucleoprotein has been demonstrated.</p></span></li><li><span>5.</span><span><p>5. DNA polymerase and RNA polymerase, purified from <em>E. coli</em> extract, had an ability to form a complex with DNA.</p></span></li></ul></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 1","pages":"Pages 36-45"},"PeriodicalIF":0.0,"publicationDate":"1964-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90168-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23779735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 52
Synthesis of 5-ribosyluracil in the liver of adrenalectomized rats treated with cortisone 可的松治疗肾上腺切除大鼠肝脏中5-核糖体尿嘧啶的合成
Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-09-11 Epub Date: 2003-02-05 DOI: 10.1016/0926-6550(64)90183-5
Kristian F. Jervell, Jan-Bjørn Osnes
{"title":"Synthesis of 5-ribosyluracil in the liver of adrenalectomized rats treated with cortisone","authors":"Kristian F. Jervell,&nbsp;Jan-Bjørn Osnes","doi":"10.1016/0926-6550(64)90183-5","DOIUrl":"10.1016/0926-6550(64)90183-5","url":null,"abstract":"","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 1","pages":"Pages 166-168"},"PeriodicalIF":0.0,"publicationDate":"1964-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90183-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23779724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Relations métaboliques entre les ribosomes libres et liés du foie de rat 大鼠肝脏中游离核糖体与结合核糖体的代谢关系
Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-09-11 Epub Date: 2003-02-05 DOI: 10.1016/0926-6550(64)90175-6
Y Moule, G.Delhumeau De Ongay
{"title":"Relations métaboliques entre les ribosomes libres et liés du foie de rat","authors":"Y Moule,&nbsp;G.Delhumeau De Ongay","doi":"10.1016/0926-6550(64)90175-6","DOIUrl":"10.1016/0926-6550(64)90175-6","url":null,"abstract":"<div><p>The interrelationships between the free and bound ribosomes of the hepatic cell in the regenerating rat liver have been studied by an examination of the metabolic relationships between RNA's of these two kinds of particles.</p><p>The kinetics of incorporation of [<sup>14</sup>C]orotic acid show no precursor-product relationship between the RNA's of free and bound ribosomes. This means that there is no systematic derivation of one kind of particle from the other. So, it is not obligatory for the life of a newly synthesized ribosome to proceed from that of a free state to that of a membrane-bound state, or inversely.</p><p>The results do not establish, however, that the two populations are independent. Indeed, the kinetics suggest the possibility of a constant exchange between them.</p><p>In addition, a precursor-product relationship has been found between nuclear RNA and ribosomal RNA. The different results are discussed.</p></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 1","pages":"Pages 113-121"},"PeriodicalIF":0.0,"publicationDate":"1964-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90175-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78997677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 24
Moving boundary electrophoretic behavior of ribonucleic acid 核糖核酸的移动边界电泳行为
Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-09-11 Epub Date: 2003-02-05 DOI: 10.1016/0926-6550(64)90167-7
Eugene L. Hess, Saima E. Lagg, T. Utsunomiya
{"title":"Moving boundary electrophoretic behavior of ribonucleic acid","authors":"Eugene L. Hess,&nbsp;Saima E. Lagg,&nbsp;T. Utsunomiya","doi":"10.1016/0926-6550(64)90167-7","DOIUrl":"10.1016/0926-6550(64)90167-7","url":null,"abstract":"<div><p>Yeast RNA has been examined, using moving-boundary electrophoretic procedures, in several buffer systems covering the pH interval 3–10 and the ionic strength range 0.025–0.5. The studies suggest that the purity of RNA is better evaluated at pH 3 and pH 10 than in the intermediate pH range. In buffers containing phosphate or cacodylate ions, lack of enantiography between patterns from ascending and descending limbs of the cell indicate RNA-buffer interactions. The mobility of RNA at the same ionic strength −10.1·10<sup>−5</sup> cm<sup>2</sup> V<sup>−1</sup> sec<sup>−1</sup> at pH 3 is 72% of the mobility, −14.0·10<sup>−5</sup> cm<sup>2</sup> V<sup>−1</sup> sec<sup>−1</sup> found at pH 10.</p></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 1","pages":"Pages 29-35"},"PeriodicalIF":0.0,"publicationDate":"1964-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90167-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23779734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Studies on the mechanism of methylcholanthrene induction of enzyme activities of rat liver 甲基胆蒽诱导大鼠肝脏酶活性的机制研究
Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-09-11 Epub Date: 2003-02-05 DOI: 10.1016/0926-6550(64)90176-8
Harry V. Gelboin, L. Sokoloff
{"title":"Studies on the mechanism of methylcholanthrene induction of enzyme activities of rat liver","authors":"Harry V. Gelboin,&nbsp;L. Sokoloff","doi":"10.1016/0926-6550(64)90176-8","DOIUrl":"https://doi.org/10.1016/0926-6550(64)90176-8","url":null,"abstract":"<div><p>The administration <em>in vivo</em> of 3-methylcholanthrene (MC) increases the rate of leucine incorporation in a nuclei-free rat-liver homogenate system. Each subcellular fraction, the mitochondria, microsomes and supernatant fluid contribute to the increased activity in the MC preparation. The major part of the incorporation takes place in the microsomal fraction. The increase in incorporation in the microsomes from MC-treated rats is not secondary to changes in the concentration of magnesium ion or GTP. Parallel changes in the activity of the supernatant fluid appear due to a more adequate maintenance of proper GTP levels in the supernatant from MC-treated rats.</p></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 1","pages":"Pages 122-129"},"PeriodicalIF":0.0,"publicationDate":"1964-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90176-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91696675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 30
The induction of mitochondrial α-glycerophosphate dehydrogenase by thyroid hormone: Evidence for enzyme synthesis 甲状腺激素诱导线粒体α-甘油磷酸脱氢酶:酶合成的证据
Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-09-11 Epub Date: 2003-02-05 DOI: 10.1016/0926-6550(64)90189-6
O.Z. Sellinger, Kai-Lin Lee
{"title":"The induction of mitochondrial α-glycerophosphate dehydrogenase by thyroid hormone: Evidence for enzyme synthesis","authors":"O.Z. Sellinger,&nbsp;Kai-Lin Lee","doi":"10.1016/0926-6550(64)90189-6","DOIUrl":"https://doi.org/10.1016/0926-6550(64)90189-6","url":null,"abstract":"","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 1","pages":"Pages 183-186"},"PeriodicalIF":0.0,"publicationDate":"1964-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90189-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91737718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 25
Polynucleotide analogues 多核苷酸类似物
Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-09-11 Epub Date: 2003-02-05 DOI: 10.1016/0926-6550(64)90173-2
A.M. Michelson, M. Grunberg-Manago
{"title":"Polynucleotide analogues","authors":"A.M. Michelson,&nbsp;M. Grunberg-Manago","doi":"10.1016/0926-6550(64)90173-2","DOIUrl":"10.1016/0926-6550(64)90173-2","url":null,"abstract":"<div><p>The effect of non-operative base analogues in polynucleotides on the ability to stimulate amino acid incorporation has been studied. Internal changes produce an effect depending on the nature and quantity of “nonsense” base. A single alteration at the nucleoside end of the polymer markedly inhibits amino acid incorporation.</p><p>Base analogues examined include xanthosine, <em>N</em><sup>1</sup>-methyluridine, <em>N</em><sup>1</sup>-methyl-5-bromouridine, <em>N</em><sup>6</sup>-hydroxyethyladenosine, <em>N</em><sup>6</sup>-hydroxymethyladenosine, <em>N</em><sup>6</sup>-methyladenosine, <em>N</em><sup>1</sup>-methyladenosine, <em>N</em><sup>6</sup>-acetylcytidine, <em>N</em><sup>6</sup>-hydroxymethylcytidine. Other variations include those obtained by treatment of polyribonucleotides with hydroxylamine, a water-soluble carbodi-imide, and periodate oxidation.</p><p>The behavior of certain analogues is sometimes dependent on the nature of the flanking nucleotides. A possible explanation for this is given.</p></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 1","pages":"Pages 92-104"},"PeriodicalIF":0.0,"publicationDate":"1964-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90173-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72524249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 49
Protein synthesis in mitochondria 线粒体中的蛋白质合成
Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-09-11 Epub Date: 2003-02-05 DOI: 10.1016/0926-6550(64)90178-1
A.M. Kroon
{"title":"Protein synthesis in mitochondria","authors":"A.M. Kroon","doi":"10.1016/0926-6550(64)90178-1","DOIUrl":"https://doi.org/10.1016/0926-6550(64)90178-1","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. A procedure for preparing rat-liver mitochondria including a pre-incubation with RNAase (EC 2.7.7.16) to inactivate the microsomal system for amino acid incorporation is described.</p></span></li><li><span>2.</span><span><p>2. The inhibition of the incorporation of amino acids into mitochondrial protein by adding substrate is correlated with a lowering of the NADP<sup>+</sup>/NADPH ratio.</p></span></li><li><span>3.</span><span><p>3. It is shown that ammonia can relieve the inhibition by α-oxoglutarate or isocitrate of the incorporation into liver mitochondria, that this effect is not due to the glutamate formed, and that this is correlated with oxidation of NADPH to NADP<sup>+</sup>.</p></span></li><li><span>4.</span><span><p>4. The role of endogenous substrate is discussed. It is concluded that in the case of rat-liver mitochondria α-oxoglutarate plus ammonia give the best conditions for providing energy for incorporation, since a high NADP<sup>+</sup>/NADPH ratio is also assured.</p></span></li><li><span>5.</span><span><p>5. Judged by the effects of oligomycin and 2,4-dinitrophenol on the amino acid incorporation into protein by mitochondria, the possibility is discussed whether and in how far the high-energy intermediates of oxidative phosphorylation can meet the energy requirements for the incorporation process.</p></span></li></ul></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 1","pages":"Pages 145-154"},"PeriodicalIF":0.0,"publicationDate":"1964-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90178-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91696676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 36
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