Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects最新文献_第10页

Ribonucleotide incorporation by a soluble enzyme from maize 由玉米可溶性酶合成的核糖核苷酸

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-09-11 DOI: 10.1016/0926-6550(64)90190-2

Rusty J. Mansf , G.David Novelli

引用次数: 14

Structure of the replicative form of bacteriophage φX174 噬菌体φX174复制形式的结构

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-09-11 DOI: 10.1016/0926-6550(64)90187-2

P.H. Pouwels, H.S. Jansz

引用次数: 33

Synthesis of 5-ribosyluracil in the liver of adrenalectomized rats treated with cortisone 可的松治疗肾上腺切除大鼠肝脏中5-核糖体尿嘧啶的合成

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-09-11 DOI: 10.1016/0926-6550(64)90183-5

Kristian F. Jervell, Jan-Bjørn Osnes

引用次数: 1

A deoxyribonucleic acid-protein complex having DNA-polymerase and RNA-polymerase activities in cell-free extracts of Escherichia coli 在大肠杆菌的无细胞提取物中具有dna聚合酶和rna聚合酶活性的脱氧核糖核酸-蛋白质复合物

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-09-11 DOI: 10.1016/0926-6550(64)90168-9

Motoko Kadoya, Hiromi Mitsui , Yasuyuki Takagi , Eiko Otaka , Hideho Suzuki , Syozo Osawa

{"title":"A deoxyribonucleic acid-protein complex having DNA-polymerase and RNA-polymerase activities in cell-free extracts of Escherichia coli","authors":"Motoko Kadoya, Hiromi Mitsui , Yasuyuki Takagi , Eiko Otaka , Hideho Suzuki , Syozo Osawa","doi":"10.1016/0926-6550(64)90168-9","DOIUrl":"10.1016/0926-6550(64)90168-9","url":null,"abstract":"<div><ul><li>1.1. A deoxyribonucleoprotein, found in extracts of Escherichia coli B(H), has been investigated with respect to its role in DNA and RNA synthesis.</li><li>2.2. A deoxyribonucleoprotein in ribosome-free cell extract could be separated from the other cellular components either with gel filtration or zone electrophoresis.</li><li>3.3. The isolated deoxyribonucleoprotein was composed of roughly 80% of DNA a and 20% of protein.</li><li>4.4. Association of DNA polymerase (deoxynucleosidetriphosphate: DNA deoxynucleotidyltransferase, EC 2.7.7.7) and RNA polymerase (nucleosidetriphosphate: RNA nucleotidyltransferase (DNA dependent), EC 2.7.7.6) with this deoxyribonucleoprotein has been demonstrated.</li><li>5.5. DNA polymerase and RNA polymerase, purified from E. coli extract, had an ability to form a complex with DNA.</li></ul></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1964-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90168-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23779735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 52

Moving boundary electrophoretic behavior of ribonucleic acid 核糖核酸的移动边界电泳行为

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-09-11 DOI: 10.1016/0926-6550(64)90167-7

Eugene L. Hess, Saima E. Lagg, T. Utsunomiya

引用次数: 2

Studies on the mechanism of methylcholanthrene induction of enzyme activities of rat liver 甲基胆蒽诱导大鼠肝脏酶活性的机制研究

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-09-11 DOI: 10.1016/0926-6550(64)90176-8

Harry V. Gelboin, L. Sokoloff

引用次数: 30

Relations métaboliques entre les ribosomes libres et liés du foie de rat 大鼠肝脏中游离核糖体与结合核糖体的代谢关系

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-09-11 DOI: 10.1016/0926-6550(64)90175-6

Y Moule, G.Delhumeau De Ongay

引用次数: 24

Requirement for continuous protein synthesis for the development of resistance to ultraviolet light in HEp-2 cells infected with Herpes simplex virus 单纯疱疹病毒感染的HEp-2细胞对紫外光产生抗性所需的连续蛋白合成

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-09-11 DOI: 10.1016/0926-6550(64)90184-7

Philip R. Roane Jr., Bernard Roizman

引用次数: 3

The inhibition of DNA polymerase by extracts of rat liver partial purification and properties of a deoxyribonuclease 大鼠肝脏部分纯化提取物对DNA聚合酶的抑制作用及脱氧核糖核酸酶的性质

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-09-11 DOI: 10.1016/0926-6550(64)90169-0

Margery G. Burdon, R.M.S. Smellie, J.N. Davidson

{"title":"The inhibition of DNA polymerase by extracts of rat liver partial purification and properties of a deoxyribonuclease","authors":"Margery G. Burdon, R.M.S. Smellie, J.N. Davidson","doi":"10.1016/0926-6550(64)90169-0","DOIUrl":"10.1016/0926-6550(64)90169-0","url":null,"abstract":"<div><ul><li>1.1. The inhibitory effect of extracts of rat liver on DNA polymerase (deoxyribonucleosidetriphosphate: DNA deoxyribonucleotidyltransferase, EC 2.7.7.7) has been investigated.</li><li>2.2. Fractionation of the crude extracts has revealed that the inhibition is not due to the hydrolysis of the deoxyribonucleoside 5′-triphosphates by phosphatases.</li><li>3.3. The inhibitory factor was purified about 10–15-fold, with a yield of 20%.</li><li>4.4. Its activity was due to the action on the primer DNA of a deoxyribonuclease, and the oligonucleotide products of the reaction inhibited the polymerase.</li><li>5.5. The properties of the deoxyribonuclease differed in some respects from those of splenic deoxyribonuclease II (deoxyribonucleate 3′-nucleotidohydrolase, EC 3.1.4.6) and pancreatic deoxyribonuclease I (deoxyribonucleate oligonucleotidohydrolase, EC 3.1.4.5). In particular, it hydrolyses thermally denatured DNA more rapidly than native DNA.</li></ul></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1964-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90169-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23779736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Protein synthesis in mitochondria 线粒体中的蛋白质合成

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-09-11 DOI: 10.1016/0926-6550(64)90178-1

A.M. Kroon

{"title":"Protein synthesis in mitochondria","authors":"A.M. Kroon","doi":"10.1016/0926-6550(64)90178-1","DOIUrl":"https://doi.org/10.1016/0926-6550(64)90178-1","url":null,"abstract":"<div><ul><li>1.1. A procedure for preparing rat-liver mitochondria including a pre-incubation with RNAase (EC 2.7.7.16) to inactivate the microsomal system for amino acid incorporation is described.</li><li>2.2. The inhibition of the incorporation of amino acids into mitochondrial protein by adding substrate is correlated with a lowering of the NADP+/NADPH ratio.</li><li>3.3. It is shown that ammonia can relieve the inhibition by α-oxoglutarate or isocitrate of the incorporation into liver mitochondria, that this effect is not due to the glutamate formed, and that this is correlated with oxidation of NADPH to NADP+.</li><li>4.4. The role of endogenous substrate is discussed. It is concluded that in the case of rat-liver mitochondria α-oxoglutarate plus ammonia give the best conditions for providing energy for incorporation, since a high NADP+/NADPH ratio is also assured.</li><li>5.5. Judged by the effects of oligomycin and 2,4-dinitrophenol on the amino acid incorporation into protein by mitochondria, the possibility is discussed whether and in how far the high-energy intermediates of oxidative phosphorylation can meet the energy requirements for the incorporation process.</li></ul></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1964-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90178-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91696676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 36