The inhibition of DNA polymerase by extracts of rat liver partial purification and properties of a deoxyribonuclease

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-09-11 DOI:10.1016/0926-6550(64)90169-0

Margery G. Burdon, R.M.S. Smellie, J.N. Davidson

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引用次数: 12

Abstract

1.
1. The inhibitory effect of extracts of rat liver on DNA polymerase (deoxyribonucleosidetriphosphate: DNA deoxyribonucleotidyltransferase, EC 2.7.7.7) has been investigated.
2.
2. Fractionation of the crude extracts has revealed that the inhibition is not due to the hydrolysis of the deoxyribonucleoside 5′-triphosphates by phosphatases.
3.
3. The inhibitory factor was purified about 10–15-fold, with a yield of 20%.
4.
4. Its activity was due to the action on the primer DNA of a deoxyribonuclease, and the oligonucleotide products of the reaction inhibited the polymerase.
5.
5. The properties of the deoxyribonuclease differed in some respects from those of splenic deoxyribonuclease II (deoxyribonucleate 3′-nucleotidohydrolase, EC 3.1.4.6) and pancreatic deoxyribonuclease I (deoxyribonucleate oligonucleotidohydrolase, EC 3.1.4.5). In particular, it hydrolyses thermally denatured DNA more rapidly than native DNA.

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大鼠肝脏部分纯化提取物对DNA聚合酶的抑制作用及脱氧核糖核酸酶的性质

1.1. 研究了大鼠肝脏提取物对DNA聚合酶(脱氧核糖核苷三磷酸:DNA脱氧核糖核苷转移酶，EC 2.7.7.7)的抑制作用。粗提取物的分馏表明，抑制作用不是由于磷酸酶水解脱氧核糖核苷5 ' -三磷酸。该抑制因子的纯度约为10 - 15倍，产率为20%。它的活性是由于作用于脱氧核糖核酸酶的引物DNA，反应的寡核苷酸产物抑制聚合酶。该脱氧核糖核酸酶的性质与脾脱氧核糖核酸酶II(脱氧核糖核酸3′-核苷酸水解酶，EC 3.1.4.6)和胰脱氧核糖核酸酶I(脱氧核糖核酸寡核苷酸水解酶，EC 3.1.4.5)在某些方面有所不同。特别是，它比天然DNA更快地水解热变性DNA。

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Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects

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