Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects最新文献

Search for repair of ultraviolet damage in mutants of bacteriophage T4 寻找T4噬菌体突变体对紫外线损伤的修复

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-12-16 DOI: 10.1016/0926-6550(64)90022-2

W. Sauerbier

引用次数: 14

Errors in the use of 260-mμ absorption for estimation of RNPr particles 用260 μ m吸收法估计RNPr粒子时的误差

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-12-16 DOI: 10.1016/0926-6550(64)90023-4

R.J. Jackson, A.J. Munro, A. Korner

引用次数: 26

Mise en évidence d'un acide ribonucléique de faible poids moléculaire dans les cellules de culture KB en phase stationnaire de croissance 在固定生长阶段KB培养细胞中发现低分子量核糖核酸

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-12-16 DOI: 10.1016/0926-6550(64)90026-X

L. Colobert, P. Louisot

引用次数: 3

Changes in sedimentation properties of ribosomal ribonucleic acids during the course of ribosome formation in Escherichia coli 大肠杆菌核糖体形成过程中核糖体核糖核酸沉降特性的变化

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-12-16 DOI: 10.1016/0926-6550(64)90009-X

M. Kono, E. Otaka, S. Osawa

{"title":"Changes in sedimentation properties of ribosomal ribonucleic acids during the course of ribosome formation in Escherichia coli","authors":"M. Kono, E. Otaka, S. Osawa","doi":"10.1016/0926-6550(64)90009-X","DOIUrl":"10.1016/0926-6550(64)90009-X","url":null,"abstract":"<div>The nascent ribosomal RNA which has not yet been incorporated into mature ribosomal particles has been reported to sediment as 17 S and 22–23 S as compared with 16 S and 23 S for RNA from mature ribosomes. In this study, we have investigated whether the transformation of sedimentation property from nascent to mature state takes place gradually during ribosome formation or occurs at the later step of ribosome maturation.Ribosomal RNA's were labelled either with [3H]adenosine or [14C]adenine at different stages of ribosome maturation, representing pulse-labelled RNA, RNA obtained in the presence of chloramphenicol or 5-fluorouracil, and RNA of mature ribosomes. Comparison of their sedimentation velocities in sucrose density gradient indicated that transformation from 17 S to 16 S occurs at the later step of ribosome maturation. No clear difference of sedimentation velocity was detected between nascent and mature 23-S ribosomal RNA in this study.</div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 4","pages":"Pages 612-618"},"PeriodicalIF":0.0,"publicationDate":"1964-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90009-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23813570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

The inhibition of deoxyribonucleotidyl transferase, DNAase and RNAase by sodium poly ethenesulfonic acid. Effect of the molecular weight of the inhibitor 聚乙烯磺酸钠对脱氧核糖核酸转移酶、脱氧核糖核酸酶和核糖核酸酶的抑制作用。抑制剂分子量的影响

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-12-16 DOI: 10.1016/0926-6550(64)90010-6

Michael K. Bach

{"title":"The inhibition of deoxyribonucleotidyl transferase, DNAase and RNAase by sodium poly ethenesulfonic acid. Effect of the molecular weight of the inhibitor","authors":"Michael K. Bach","doi":"10.1016/0926-6550(64)90010-6","DOIUrl":"10.1016/0926-6550(64)90010-6","url":null,"abstract":"<div><ul><li>1.1. The nature of the inhibition of DNA nucleotidyltransferase (EC 2.7.7.7) from KB cells (DNA polymerase) by poly ethenesulfonic acid (PES) and other polyanionic materials were examined. The inhibition was found to be competitive with the DNA used to prime the enzyme. The K1 was found to be 1.72 ± 0.36 μg PES/ml for the 12 900 mol. wt. material in the range of zero to 10 μg inhibitor/ml. The inhibitory action of PES was strongly dependent on the molecular weight of the sample used in the range of 5000 to 13 000 and further increases in molecular weight did not seem to affect efficacy. Other polyanionic polymers, such as heparin, carboxymethyl cellulose, alginic acid, chitosan sulfate, RNA and chondroitin sulfate also inhibited this enzyme to varying degrees.</li><li>2.2. Spermine, which by itself inhibits the DNA polymerase, reversed the inhibition caused by PES. This is explained by the competition between DNA and PES for salt formation (and precipitation) with spermine.</li><li>3.3. The inhibition of pancreatic RNAase (EC 2.7.7.16) by PES exhibited a similar dependence on the molecular weight of the polymer as did the inhibition of DNA polymerase.</li><li>4.4. Addition of PES to DNAase I (EC 3.1.4.5) assays resulted in a bimodal, concentration-dependent curve. At intermediate concentrations the compound stimulated DNA hydrolysis, while at higher concentrations it inhibited.</li></ul></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 4","pages":"Pages 619-626"},"PeriodicalIF":0.0,"publicationDate":"1964-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90010-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23813571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Separation and some properties of distinct classes of newly-formed ribonucleic acid from animal cells 动物细胞中不同种类新形成的核糖核酸的分离和一些特性

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-12-16 DOI: 10.1016/0926-6550(64)90027-1

G.P. Georgiev , M.I. Lerman

引用次数: 22

Fractionation of DNA II. Partial strand separation as a basis of fractionation by countercurrent distribution DNA的分离II。部分链分离作为逆流分布分馏的基础

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-12-16 DOI: 10.1016/0926-6550(64)90011-8

Chev Kidson, K.S. Kirby

引用次数: 8

Effect of purines and some of their analogues on protein and RNA synthesis in isolated nuclei 嘌呤及其类似物对分离细胞核中蛋白质和RNA合成的影响

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-12-16 DOI: 10.1016/0926-6550(64)90024-6

J. Malec , L. Kornacka , M. Wojnarowska , K. Zakrzewski

引用次数: 4

Messenger ribonucleic acid activity in rabbit reticulocyte ribosomes 兔网织细胞核糖体信使核糖核酸活性

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-12-16 DOI: 10.1016/0926-6550(64)90028-3

John C. Drach, Jerry B. Lingrel

引用次数: 17

Spaltung einer serinspezifischen transfer-ribonucleinsäure-fraktion mit T1-ribonuclease 巴斯克分离党

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-12-16 DOI: 10.1016/0926-6550(64)90004-0

D. Dütting, H.G. Zachau

引用次数: 13