The inhibition of deoxyribonucleotidyl transferase, DNAase and RNAase by sodium poly ethenesulfonic acid. Effect of the molecular weight of the inhibitor

Michael K. Bach
{"title":"The inhibition of deoxyribonucleotidyl transferase, DNAase and RNAase by sodium poly ethenesulfonic acid. Effect of the molecular weight of the inhibitor","authors":"Michael K. Bach","doi":"10.1016/0926-6550(64)90010-6","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. The nature of the inhibition of DNA nucleotidyltransferase (EC 2.7.7.7) from KB cells (DNA polymerase) by poly ethenesulfonic acid (PES) and other polyanionic materials were examined. The inhibition was found to be competitive with the DNA used to prime the enzyme. The <em>K</em><sub>1</sub> was found to be 1.72 ± 0.36 <em>μg</em> PES/ml for the 12 900 mol. wt. material in the range of zero to 10 μg inhibitor/ml. The inhibitory action of PES was strongly dependent on the molecular weight of the sample used in the range of 5000 to 13 000 and further increases in molecular weight did not seem to affect efficacy. Other polyanionic polymers, such as heparin, carboxymethyl cellulose, alginic acid, chitosan sulfate, RNA and chondroitin sulfate also inhibited this enzyme to varying degrees.</p></span></li><li><span>2.</span><span><p>2. Spermine, which by itself inhibits the DNA polymerase, reversed the inhibition caused by PES. This is explained by the competition between DNA and PES for salt formation (and precipitation) with spermine.</p></span></li><li><span>3.</span><span><p>3. The inhibition of pancreatic RNAase (EC 2.7.7.16) by PES exhibited a similar dependence on the molecular weight of the polymer as did the inhibition of DNA polymerase.</p></span></li><li><span>4.</span><span><p>4. Addition of PES to DNAase I (EC 3.1.4.5) assays resulted in a bimodal, concentration-dependent curve. At intermediate concentrations the compound stimulated DNA hydrolysis, while at higher concentrations it inhibited.</p></span></li></ul></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 4","pages":"Pages 619-626"},"PeriodicalIF":0.0000,"publicationDate":"1964-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90010-6","citationCount":"21","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926655064900106","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 21

Abstract

  • 1.

    1. The nature of the inhibition of DNA nucleotidyltransferase (EC 2.7.7.7) from KB cells (DNA polymerase) by poly ethenesulfonic acid (PES) and other polyanionic materials were examined. The inhibition was found to be competitive with the DNA used to prime the enzyme. The K1 was found to be 1.72 ± 0.36 μg PES/ml for the 12 900 mol. wt. material in the range of zero to 10 μg inhibitor/ml. The inhibitory action of PES was strongly dependent on the molecular weight of the sample used in the range of 5000 to 13 000 and further increases in molecular weight did not seem to affect efficacy. Other polyanionic polymers, such as heparin, carboxymethyl cellulose, alginic acid, chitosan sulfate, RNA and chondroitin sulfate also inhibited this enzyme to varying degrees.

  • 2.

    2. Spermine, which by itself inhibits the DNA polymerase, reversed the inhibition caused by PES. This is explained by the competition between DNA and PES for salt formation (and precipitation) with spermine.

  • 3.

    3. The inhibition of pancreatic RNAase (EC 2.7.7.16) by PES exhibited a similar dependence on the molecular weight of the polymer as did the inhibition of DNA polymerase.

  • 4.

    4. Addition of PES to DNAase I (EC 3.1.4.5) assays resulted in a bimodal, concentration-dependent curve. At intermediate concentrations the compound stimulated DNA hydrolysis, while at higher concentrations it inhibited.

聚乙烯磺酸钠对脱氧核糖核酸转移酶、脱氧核糖核酸酶和核糖核酸酶的抑制作用。抑制剂分子量的影响
1.1. 研究了聚乙烯磺酸(PES)和其他聚阴离子材料对KB细胞DNA聚合酶(DNA nucleotidyltransferase, EC 2.7.7.7)的抑制作用。抑制作用被发现与用于引物酶的DNA竞争。在0 ~ 10 μg抑制剂/ml范围内,12 900 mol. wt材料的K1为1.72±0.36 μg PES/ml。PES的抑制作用强烈依赖于所使用样品的分子量,在5000至13000范围内,分子量的进一步增加似乎不影响效果。其他聚阴离子聚合物,如肝素、羧甲基纤维素、海藻酸、硫酸壳聚糖、RNA和硫酸软骨素也不同程度地抑制该酶。精胺本身可以抑制DNA聚合酶,逆转了PES引起的抑制作用。这可以解释为DNA和PES在与精胺形成盐(和沉淀)方面的竞争。PES对胰腺RNAase (EC 2.7.7.16)的抑制作用与对DNA聚合酶的抑制作用同样依赖于聚合物的分子量。将PES添加到DNAase I (EC 3.1.4.5)检测中,产生双峰浓度依赖性曲线。在中等浓度下,该化合物刺激DNA水解,而在较高浓度下则抑制DNA水解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信