Spaltung einer serinspezifischen transfer-ribonucleinsäure-fraktion mit T1-ribonuclease

Abstract

Soluble RNA and a serine specific transfer RNA fraction were digested by highly purified T₁-RNAase (ribonucleate 3′-guanylohydrolase, EC 3.1.4.8) and phosphomonoesterase (orthophosphoric monoester phosphohydrolase, EC 3.1.3.1). Conditions for the separation of the oligonucleotides on DEAE-cellulose columns were worked out with digests of soluble RNA (Fig. 1–3). The splitting products of the serine-transfer-RNA fraction were separated (Fig. 4) and further purified by paper electrophoresis. Their nucleotide composition — including the odd nucleotides — was determined by conventional methods and the sequences partially elucidated. 5 Di-, 6 tri-, 7 tetra-, 2 penta- and 1 hexanucleotide of the two serine-transfer-RNA chains were identified (Table I) and quantitatively determined (Table II). One longer oligonucleotide was partially identified. Some oligonucleotides, which are present in small amounts, were due to impurities in the serine-transfer-RNA sample.