Fractionation of DNA II. Partial strand separation as a basis of fractionation by countercurrent distribution

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-12-16 DOI:10.1016/0926-6550(64)90011-8

Chev Kidson, K.S. Kirby

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引用次数: 8

Abstract

Fractionation of native DNA from mammalian and bacterial sources by countercurrent distribution has been found to depend on the degree of partial separation of the two strands. This is indicated by thermal denaturation of countercurrent distribution fractions of DNA and by countercurrent distribution of DNA reacted with [¹⁴C]formaldehyde. Countercurrent distribution of DNA double-labelled with [¹⁴C]thymidine and [³H]deoxycytidine, and measurements of T_m and buoyant density in CsCl of countercurrent distribution fractions of DNA indicate that base composition does not play an important part in the separation. Countercurrent distribution fractions of DNA did not show significant differences in s values. Countercurrent distribution in the solvent system used may exaggerate preexistent partial denaturation but it is considered that the patterns obtained do reflect the amount of denaturation which is present in the DNA in the cell.

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DNA的分离II。部分链分离作为逆流分布分馏的基础

通过逆流分布从哺乳动物和细菌来源分离原生DNA，已发现依赖于两股部分分离的程度。这可以通过DNA逆流分布部分的热变性和DNA与[14C]甲醛反应的逆流分布来表明。[14C]胸腺嘧啶和[3H]脱氧胞苷双标记DNA的逆流分布，以及DNA逆流分布部分的CsCl中Tm和浮力密度的测量表明，碱基组成在分离中不起重要作用。DNA逆流分布分数在s值上无显著差异。所使用的溶剂体系中的逆流分布可能会夸大先前存在的部分变性，但我们认为所获得的模式确实反映了细胞中DNA中存在的变性量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects

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