甲基胆蒽诱导大鼠肝脏酶活性的机制研究

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects Pub Date : 1964-09-11 DOI:10.1016/0926-6550(64)90176-8

Harry V. Gelboin, L. Sokoloff

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引用次数: 30

摘要

体内给药3-甲基胆蒽(MC)增加了亮氨酸在无核大鼠肝脏匀浆系统中的掺入率。每个亚细胞部分，线粒体，微粒体和上清液有助于增加MC制备中的活性。合并的主要部分发生在微粒体部分。mc处理大鼠微粒体中掺入量的增加不是继发于镁离子或GTP浓度的变化。由于mc处理大鼠的上清液中更充分地维持了适当的GTP水平，因此上清液的活性也发生了平行变化。

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Studies on the mechanism of methylcholanthrene induction of enzyme activities of rat liver

The administration in vivo of 3-methylcholanthrene (MC) increases the rate of leucine incorporation in a nuclei-free rat-liver homogenate system. Each subcellular fraction, the mitochondria, microsomes and supernatant fluid contribute to the increased activity in the MC preparation. The major part of the incorporation takes place in the microsomal fraction. The increase in incorporation in the microsomes from MC-treated rats is not secondary to changes in the concentration of magnesium ion or GTP. Parallel changes in the activity of the supernatant fluid appear due to a more adequate maintenance of proper GTP levels in the supernatant from MC-treated rats.

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Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects

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