{"title":"在大肠杆菌的无细胞提取物中具有dna聚合酶和rna聚合酶活性的脱氧核糖核酸-蛋白质复合物","authors":"Motoko Kadoya, Hiromi Mitsui , Yasuyuki Takagi , Eiko Otaka , Hideho Suzuki , Syozo Osawa","doi":"10.1016/0926-6550(64)90168-9","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. A deoxyribonucleoprotein, found in extracts of <em>Escherichia coli</em> B(H), has been investigated with respect to its role in DNA and RNA synthesis.</p></span></li><li><span>2.</span><span><p>2. A deoxyribonucleoprotein in ribosome-free cell extract could be separated from the other cellular components either with gel filtration or zone electrophoresis.</p></span></li><li><span>3.</span><span><p>3. The isolated deoxyribonucleoprotein was composed of roughly 80% of DNA a and 20% of protein.</p></span></li><li><span>4.</span><span><p>4. Association of DNA polymerase (deoxynucleosidetriphosphate: DNA deoxynucleotidyltransferase, EC 2.7.7.7) and RNA polymerase (nucleosidetriphosphate: RNA nucleotidyltransferase (DNA dependent), EC 2.7.7.6) with this deoxyribonucleoprotein has been demonstrated.</p></span></li><li><span>5.</span><span><p>5. DNA polymerase and RNA polymerase, purified from <em>E. coli</em> extract, had an ability to form a complex with DNA.</p></span></li></ul></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 1","pages":"Pages 36-45"},"PeriodicalIF":0.0000,"publicationDate":"1964-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90168-9","citationCount":"52","resultStr":"{\"title\":\"A deoxyribonucleic acid-protein complex having DNA-polymerase and RNA-polymerase activities in cell-free extracts of Escherichia coli\",\"authors\":\"Motoko Kadoya, Hiromi Mitsui , Yasuyuki Takagi , Eiko Otaka , Hideho Suzuki , Syozo Osawa\",\"doi\":\"10.1016/0926-6550(64)90168-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p></p><ul><li><span>1.</span><span><p>1. A deoxyribonucleoprotein, found in extracts of <em>Escherichia coli</em> B(H), has been investigated with respect to its role in DNA and RNA synthesis.</p></span></li><li><span>2.</span><span><p>2. A deoxyribonucleoprotein in ribosome-free cell extract could be separated from the other cellular components either with gel filtration or zone electrophoresis.</p></span></li><li><span>3.</span><span><p>3. The isolated deoxyribonucleoprotein was composed of roughly 80% of DNA a and 20% of protein.</p></span></li><li><span>4.</span><span><p>4. Association of DNA polymerase (deoxynucleosidetriphosphate: DNA deoxynucleotidyltransferase, EC 2.7.7.7) and RNA polymerase (nucleosidetriphosphate: RNA nucleotidyltransferase (DNA dependent), EC 2.7.7.6) with this deoxyribonucleoprotein has been demonstrated.</p></span></li><li><span>5.</span><span><p>5. DNA polymerase and RNA polymerase, purified from <em>E. coli</em> extract, had an ability to form a complex with DNA.</p></span></li></ul></div>\",\"PeriodicalId\":100173,\"journal\":{\"name\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects\",\"volume\":\"91 1\",\"pages\":\"Pages 36-45\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1964-09-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0926-6550(64)90168-9\",\"citationCount\":\"52\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0926655064901689\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926655064901689","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A deoxyribonucleic acid-protein complex having DNA-polymerase and RNA-polymerase activities in cell-free extracts of Escherichia coli
1.
1. A deoxyribonucleoprotein, found in extracts of Escherichia coli B(H), has been investigated with respect to its role in DNA and RNA synthesis.
2.
2. A deoxyribonucleoprotein in ribosome-free cell extract could be separated from the other cellular components either with gel filtration or zone electrophoresis.
3.
3. The isolated deoxyribonucleoprotein was composed of roughly 80% of DNA a and 20% of protein.
4.
4. Association of DNA polymerase (deoxynucleosidetriphosphate: DNA deoxynucleotidyltransferase, EC 2.7.7.7) and RNA polymerase (nucleosidetriphosphate: RNA nucleotidyltransferase (DNA dependent), EC 2.7.7.6) with this deoxyribonucleoprotein has been demonstrated.
5.
5. DNA polymerase and RNA polymerase, purified from E. coli extract, had an ability to form a complex with DNA.
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