Moving boundary electrophoretic behavior of ribonucleic acid

Eugene L. Hess, Saima E. Lagg, T. Utsunomiya
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引用次数: 2

Abstract

Yeast RNA has been examined, using moving-boundary electrophoretic procedures, in several buffer systems covering the pH interval 3–10 and the ionic strength range 0.025–0.5. The studies suggest that the purity of RNA is better evaluated at pH 3 and pH 10 than in the intermediate pH range. In buffers containing phosphate or cacodylate ions, lack of enantiography between patterns from ascending and descending limbs of the cell indicate RNA-buffer interactions. The mobility of RNA at the same ionic strength −10.1·10−5 cm2 V−1 sec−1 at pH 3 is 72% of the mobility, −14.0·10−5 cm2 V−1 sec−1 found at pH 10.

核糖核酸的移动边界电泳行为
采用移动边界电泳方法,在pH区间3-10和离子强度范围0.025-0.5的几种缓冲系统中对酵母RNA进行了检测。研究表明,在pH 3和pH 10下,RNA的纯度比在中间pH范围内更好。在含有磷酸盐或羧酸盐离子的缓冲液中,来自细胞上升和下降分支的模式之间缺乏对映图表明rna -缓冲液相互作用。在相同离子强度下,RNA在pH值为- 10.1·10−5 cm2 V−1 sec−1时的迁移率是pH值为- 14.0·10−5 cm2 V−1 sec−1时的72%。
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