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Optimizing Hydrogen Direct Injection to Overcome Design Challenges in Jet Ignition Systems 优化氢直接喷射,克服喷气点火系统的设计挑战
Applied Research Pub Date : 2025-04-16 DOI: 10.1002/appl.70011
Alberto Boretti
{"title":"Optimizing Hydrogen Direct Injection to Overcome Design Challenges in Jet Ignition Systems","authors":"Alberto Boretti","doi":"10.1002/appl.70011","DOIUrl":"https://doi.org/10.1002/appl.70011","url":null,"abstract":"<div>\u0000 \u0000 <p>Hydrogen direct injection (DI) is a critical technology for advancing internal combustion engines (ICEs) as efficient, low-emission alternatives in a sustainable energy future. While high-pressure direct injection (HPDI) offers significant advantages in efficiency and power density—particularly when paired with jet ignition systems—it remains underdeveloped compared to port fuel injection (PFI) and low-pressure DI (LPDI) systems. This study identifies the limitations of existing HPDI injector designs, including insufficient injection pressures and mass flow rates, and emphasizes the need for single-fuel HPDI systems. A novel method utilizing cryogenic hydrogen warmed to ambient temperature in a constant-volume chamber is proposed to achieve higher injection pressures without added complexity. When integrated with jet ignition, this approach delivers brake thermal efficiencies of up to 50% across diverse operating conditions, far surpassing PFI and LPDI. The findings underscore the urgent need for investment in HPDI technology to unlock the full potential of hydrogen ICEs, enhancing efficiency, power density, and sustainability.</p></div>","PeriodicalId":100109,"journal":{"name":"Applied Research","volume":"4 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/appl.70011","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143835936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Realistic Modeling of Photovoltaic Solar Cell: A Simple and Accurate Two-Diode Model 光伏太阳能电池的逼真建模:一个简单而精确的双二极管模型
Applied Research Pub Date : 2025-04-09 DOI: 10.1002/appl.70010
Jordan Nafack Nihako, Elie Simo, Derrick Duclos Abada Essouma, Maëlle Nanmegne Leutchouang, Christel Roseny Atteutsia Tsakem, Christelle Yolande Tchienou Tchienou, Jimy Synclair Kenhago Watia, Pierre-Olivier Logerais, Joseph Marae Djouda
{"title":"Realistic Modeling of Photovoltaic Solar Cell: A Simple and Accurate Two-Diode Model","authors":"Jordan Nafack Nihako,&nbsp;Elie Simo,&nbsp;Derrick Duclos Abada Essouma,&nbsp;Maëlle Nanmegne Leutchouang,&nbsp;Christel Roseny Atteutsia Tsakem,&nbsp;Christelle Yolande Tchienou Tchienou,&nbsp;Jimy Synclair Kenhago Watia,&nbsp;Pierre-Olivier Logerais,&nbsp;Joseph Marae Djouda","doi":"10.1002/appl.70010","DOIUrl":"https://doi.org/10.1002/appl.70010","url":null,"abstract":"<p>Photovoltaic modules are determinant in producing sustainable energy with a reduced environmental impact. This article explores the progressive modeling of photovoltaic modules, from the straightforward but approximate one-diode model to the more accurate but more complex two-diode model. It focuses on the parameters to be considered and the judicious choice of hypotheses to obtain electrical behavior close to that obtained experimentally for different environmental conditions. A reverse coupled saturation current and the Newton−Raphson method are both used for theoretical calculation and the simulation, respectively. Simulations show that the root mean square error (RMSE) on the I–V curves is reduced by 11.2% for irradiance of 1000 W/m² and by 28.3% on the P–V curves at 60°C. Additionally, the parallel resistance estimated with the two-diode model is lower than with the single-diode model (310 to 110.8 Ω), indicating a better consideration of leakage currents. Although the computation time is increased by around 40%, the improvement in accuracy justifies this added complexity. In conclusion, the study confirms the relevance of the two-diode model for a more realistic representation of photovoltaic module performance under various environmental conditions.</p>","PeriodicalId":100109,"journal":{"name":"Applied Research","volume":"4 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/appl.70010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143801922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimization and Scalability of Polymer-Modified PSCs Investigated by Machine Learning 基于机器学习的聚合物改性PSCs优化与可扩展性研究
Applied Research Pub Date : 2025-04-06 DOI: 10.1002/appl.70009
Mohamed M. Elsenety, Christos Falaras, Elias Stathatos, Yunjuan Niu, Linhua Hu
{"title":"Optimization and Scalability of Polymer-Modified PSCs Investigated by Machine Learning","authors":"Mohamed M. Elsenety,&nbsp;Christos Falaras,&nbsp;Elias Stathatos,&nbsp;Yunjuan Niu,&nbsp;Linhua Hu","doi":"10.1002/appl.70009","DOIUrl":"https://doi.org/10.1002/appl.70009","url":null,"abstract":"<div>\u0000 \u0000 <p>Advanced engineering strategies are employed to optimize the performance of perovskite solar cells (PSCs). In this study, the introduction of polyvinylpyrrolidone (PVP) to the MAPbI<sub>3</sub> perovskite precursor results in PSCs presenting self-healing ability in a moisture environment and power conversion efficiency (PCE) of up to 20.35%. We utilize machine learning to correlate comprehensive J–V experimental data with corresponding photovoltaic parameters. We identify key factors and correlations of J<sub>sc</sub>, FF, and V<sub>oc</sub> that primarily influence the PCE and scalability of polymer-modified PSCs. The findings indicated that the correlation between PCE and active area (AE) drops from 40% in reference cells to approximately 1% in the modified cells with PVP, justifying the scale-up potential of the modified approach. This is not the case for untreated devices, where PCE is largely affected by shunt (R<sub>sh</sub>) and series (R<sub>s</sub>) resistances. We evaluated 25 different algorithms through cross-validation, with the Gaussian Process emerging as the best-performing model, achieving an <i>R</i><sup>2</sup> of 0.94 and minimal errors. This model/algorithm was applied to optimize the fabrication process by predicting the optimal amount of PVP, which was determined to be 4.5 mg/L, and predicting the corresponding current–voltage (J–V) characteristics as well. This study offers a robust framework for systematically designing and optimizing durable and scalable polymer-modified PSCs, advancing the field of third-generation photovoltaic technology.</p></div>","PeriodicalId":100109,"journal":{"name":"Applied Research","volume":"4 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/appl.70009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143786818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Copper (I)-Based Metallo-Supramolecular Polymer for Nonenzymatic Electrochemical Detection of Nitrite 用于亚硝酸盐非酶电化学检测的铜基金属超分子聚合物
Applied Research Pub Date : 2025-03-31 DOI: 10.1002/appl.70008
Protity Saha, Riva Akter, Nahid Naowaz, S. M. Abu Nayem, Abdul Awal, Delwar Hossain, A. J. Saleh Ahammad
{"title":"Copper (I)-Based Metallo-Supramolecular Polymer for Nonenzymatic Electrochemical Detection of Nitrite","authors":"Protity Saha,&nbsp;Riva Akter,&nbsp;Nahid Naowaz,&nbsp;S. M. Abu Nayem,&nbsp;Abdul Awal,&nbsp;Delwar Hossain,&nbsp;A. J. Saleh Ahammad","doi":"10.1002/appl.70008","DOIUrl":"https://doi.org/10.1002/appl.70008","url":null,"abstract":"<div>\u0000 \u0000 <p>The electrochemical detection of nitrite, known for its environmental and health hazards, has been a focal point of research. A novel Cu(I)-based metallo-supramolecular polymer (SMP) (polyCu) was developed for nitrite detection using a symmetrical hexadentate terpyridine ligand[4’,4”“-(1,4-phenylene) bis(2,2’:6’,2”-terpyridine)] ligand in 1:1 ratio. The Cu(I) complexation with the ligand was confirmed through UV-Vis spectroscopy, and the prepared polyCu polymer was characterized using field-emission scanning electron microscopy (FESEM) and energy dispersive X-ray spectroscopy (EDS) techniques. The viscosity measurement of polyCu was utilized to calculate its molecular weight using Mark–Houwink-Sakurada equation. Electrochemical analysis, including cyclic voltammetry (CV) and differential pulse voltammetry (DPV), revealed excellent activity in nitrite sensing, with a well-defined peak. DPV exhibited a linear range of 1–500 μM and a low limit of detection (LOD) of 2.378 μM. The sensing mechanism was predicted based on the literature review. The polyCu_GCE demonstrated high selectivity, stability, and repeatability, making it a reliable electrocatalyst. Real sample analysis affirmed its practical applicability, positioning the sensor as a cost-effective and dependable system for nitrite sensing.</p></div>","PeriodicalId":100109,"journal":{"name":"Applied Research","volume":"4 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/appl.70008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Microscale Fabrication on Multi-Directional Mechanical Properties of Additively Manufactured Poly Lactic Acid With Grid Infills 微尺度加工对网格填充增材制聚乳酸多向力学性能的影响
Applied Research Pub Date : 2025-03-02 DOI: 10.1002/appl.70006
M. Abolfathi, F. Moroni, A. Pirondi, E. Bedogni
{"title":"Effect of Microscale Fabrication on Multi-Directional Mechanical Properties of Additively Manufactured Poly Lactic Acid With Grid Infills","authors":"M. Abolfathi,&nbsp;F. Moroni,&nbsp;A. Pirondi,&nbsp;E. Bedogni","doi":"10.1002/appl.70006","DOIUrl":"https://doi.org/10.1002/appl.70006","url":null,"abstract":"<p>In additive manufacturing, infill patterns have a significant impact on both printing time and mechanical performance, creating a necessary trade-off between the two from an industrial perspective. This study aims therefore to find an easy-to-handle procedure for rapid evaluation of the influence of infill density and raster angle on the elastic properties of 3D-printed components, from the perspective of their adoption in the industrial process of component design. In particular, the study's goal is to predict the elastic modulus in three directions. Tensile tests were carried out on bulk specimens according to ISO 527 to determine the elastic properties of 3D-printed PLA necessary for the numerical analysis. Cubic specimens were then manufactured with three densities (20%, 40%, and 60%) and two raster angles (−45°/45° and 0°/90°). Quasi-static compression tests were conducted on those specimens to assess their homogenized elastic behavior in three directions. One important result of the experimental phase was the relationship between Young's modulus (E) in the three directions. The average of E in directions 1 and 2 (build plate) is named E<sub>1,2</sub> and on the build-up directions is E<sub>3</sub>, for α = 0°/90° was E<sub>1,2</sub> = 0.8E<sub>3</sub> and for <i>α</i> = −45°/45° was E<sub>1,2</sub> = 0.28E<sub>3</sub>. Three finite element models were developed and run with the elastic properties determined by tensile tests, namely: (a) a shell model (SHL) where the internal and external walls of the specimens were modeled using shell elements with the nominal geometry; (b) a solid model (SLD) with the nominal geometry and (c) a nonuniform section model (NUS) in which the geometry was taken from microscope image to account for manufacturing imperfections. The difference between simulation and experiment for SHL was 19%, SLD was 15%, and NUS was 13%, indicating an overall good correspondence and, at the same time, that the real geometry resulting from the manufacturing process has a non-negligible impact on the homogenized value. Besides validating the values and relationships, FEM elucidated which sections of the cubes experienced stress and contributed to stiffness under various patterns and loading scenarios.</p>","PeriodicalId":100109,"journal":{"name":"Applied Research","volume":"4 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/appl.70006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143530203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cover Image: Volume 4 Issue 2 封面图片:第四卷第二期
Applied Research Pub Date : 2025-02-27 DOI: 10.1002/appl.202570002
{"title":"Cover Image: Volume 4 Issue 2","authors":"","doi":"10.1002/appl.202570002","DOIUrl":"https://doi.org/10.1002/appl.202570002","url":null,"abstract":"<p><i>Applied Research</i> is a multidisciplinary journal that focuses on bridging fundamental research and practical applications, supporting sustainable problem-solving and global initiatives. The journal covers high-quality research in fields such as Materials, Applied Physics, Chemistry, Applied Biology, Food Science, Engineering, Biomedical Sciences, and Social Sciences. Authors can submit various article types, including Reviews, Tutorials, and Research Articles. The journal aims to highlight innovative research that demonstrates the application of knowledge, methods, instrumentation, and technology into solutions.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":100109,"journal":{"name":"Applied Research","volume":"4 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/appl.202570002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143513598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessing the Sustainability of Wooden Wind Turbine Blades and Towers Compared to Conventional Designs 与传统设计相比,木质风力涡轮机叶片和塔的可持续性评估
Applied Research Pub Date : 2025-02-27 DOI: 10.1002/appl.70007
Alberto Boretti
{"title":"Assessing the Sustainability of Wooden Wind Turbine Blades and Towers Compared to Conventional Designs","authors":"Alberto Boretti","doi":"10.1002/appl.70007","DOIUrl":"https://doi.org/10.1002/appl.70007","url":null,"abstract":"<div>\u0000 \u0000 <p>This study evaluates the environmental sustainability of wooden wind turbine blades and towers in comparison to conventional materials, focusing on lifecycle CO2 emissions, production energy, and recyclability. By analyzing components made from laminated veneer lumber, the assessment reveals that wooden blades can reduce CO<sub>2</sub> emissions by as much as 80% relative to traditional fiberglass and epoxy designs. Wooden towers, designed in modular segments, exhibit up to a 66% reduction in lifecycle emissions when compared to steel towers, along with improved transport and assembly efficiencies. These findings highlight the potential for wood-based turbine components to advance sustainable energy through reduced environmental impact and the use of renewable resources, offering a promising alternative in the transition to eco-friendly energy infrastructure.</p></div>","PeriodicalId":100109,"journal":{"name":"Applied Research","volume":"4 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/appl.70007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143513826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of Bi2O3 Concentration on Optical and Gamma Ray Shielding Properties of BaTiO3 Ceramics Bi2O3浓度对BaTiO3陶瓷光学和γ射线屏蔽性能的影响
Applied Research Pub Date : 2025-02-05 DOI: 10.1002/appl.70001
Ramakumar Nodagala, Tejeswara Rao Ponnada
{"title":"Influence of Bi2O3 Concentration on Optical and Gamma Ray Shielding Properties of BaTiO3 Ceramics","authors":"Ramakumar Nodagala,&nbsp;Tejeswara Rao Ponnada","doi":"10.1002/appl.70001","DOIUrl":"https://doi.org/10.1002/appl.70001","url":null,"abstract":"<p>This study elaborated on the influence of bismuth oxide (Bi<sub>2</sub>O<sub>3</sub>, Bi) on optical and radiation shielding properties of Barium titanate (BaTiO<sub>3</sub>, BTO) when added with different wt% concentrations. To study these properties, BaTiO<sub>3</sub>–xBi<sub>2</sub>O<sub>3</sub>; x = 0,2,4,6 and 8 wt% ceramics samples were fabricated via solid state reaction method. The optical properties of prepared samples were inspected with the help of the UV–Vis technique. The absorption coefficient increased while transmittance decreased with increasing the wt% of Bi content. Samples show a decrement in indirect optical bandgap values from 3.44 to 3.35 eV while direct bandgap from 3.19 to 3.02 eV when Bi content increases from x = 0 wt% to 8 wt%. The other optical parameters, such as Urbach energy, refractive index, extinction coefficient, and dielectric constant, were also calculated. The FESEM (field emission scanning electron microscope) technique was used to identify the homogeneity in the samples. The prepared samples were tested at 356, 511, 600, 1173, 1275, and 1333 keV energies to estimate radiation shielding properties with radioactive sources 133<sub>Ba</sub>, 22<sub>Na</sub>, 137<sub>Cs</sub>, and 60<sub>Co</sub>. As Bi content increased in prepared samples, the mass attenuation coefficient (MAC) increased. At energy 356 keV, the observed MAC values are 12.685, 12.983, 13.282, 13.58, and 13.898 cm<sup>2</sup>/g while at 1333 keV, the values noticed as 5.054, 5.066, 5.079, 5.091, and 5.103 cm<sup>2</sup>/g as Bi content increased from x = 0 wt% to x = 8 wt%. Both atomic cross-section (ACS) and electronic cross-section (ECS) were calculated. ACS values are improved from 9.825 to 11.1967 barn/atom while the ECS values enhanced from 3.8949 to 4.0226 barn/electron at 356 keV as Bi content increased from x = 0 wt% to x = 8 wt%. This similar trend was observed at other energies (511, 600, 1173, and 1275 keV) for all prepared samples. The theoretical values obtained from Phy-X/PSD software were compared with calculated values and found a close agreement between them. From results, it was clear that prepared samples showed enhanced optical and radiation shielding properties when Bi content increased in BTO ceramics.</p>","PeriodicalId":100109,"journal":{"name":"Applied Research","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/appl.70001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143248774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Novel Impedance Platform Based on Printed Polymer Electrodes for Automated Virus Neutralization Assays 一种基于印刷聚合物电极的新型阻抗平台用于自动病毒中和检测
Applied Research Pub Date : 2025-02-05 DOI: 10.1002/appl.70004
Stefanie Michaelis, Anja Germann, Marcus Schäfer, Jannik Jungmann, Anne-Kathrin Mildner, Iris Riemann, Saskia Bast, Thorsten Knoll, Sylvia Wagner, Eike Kottkamp, Daniel Baasner, Boris Anczykowski, Joachim Wegener
{"title":"A Novel Impedance Platform Based on Printed Polymer Electrodes for Automated Virus Neutralization Assays","authors":"Stefanie Michaelis,&nbsp;Anja Germann,&nbsp;Marcus Schäfer,&nbsp;Jannik Jungmann,&nbsp;Anne-Kathrin Mildner,&nbsp;Iris Riemann,&nbsp;Saskia Bast,&nbsp;Thorsten Knoll,&nbsp;Sylvia Wagner,&nbsp;Eike Kottkamp,&nbsp;Daniel Baasner,&nbsp;Boris Anczykowski,&nbsp;Joachim Wegener","doi":"10.1002/appl.70004","DOIUrl":"https://doi.org/10.1002/appl.70004","url":null,"abstract":"<p>Cell-based neutralization assays are of central importance for the development of new vaccine candidates as well as quality assurance of already approved vaccines. Suppression of viral infection by neutralizing antibodies present in serum of vaccinated individuals serves as an indicator for efficacy of a vaccine. Established readouts used to date are hardly automated, provide no time resolution and require expensive reagents. These shortcomings are limiting factors in vaccine development. In contrast, when virus-compatible host cells are grown on multi-electrode arrays, the cellular infection state and the associated cell response are assessable by impedance measurements. Unlike endpoint assays, the host cell response is followed continuously in real time, label-free and noninvasively. Here, a sensor platform comprising hardware, software and disposable electrode arrays is described suitable for fully automated cell-based neutralization assays tailored for high throughput screening campaigns. To develop cost-effective, disposable electrode arrays for impedance measurements, we screen printed film electrodes made from conducting polymers on the bottom of multi-well plates. The polymer electrodes were characterized for their host cell compatibility and readout performance in comparison to established gold-film electrodes. Hard- and software were tailored for robust and routine use in virological assays. Virus titration, virus neutralization as well as antiviral drug (Efavirenz) intervention studies were conducted using vesicular stomatitis virus (VSV) pseudotypes or the Env HIV-1 infectious molecular clones Ce1176 and X1632 as viral model systems. The assays showed very similar analytical performance in terms of titration curves and dose–response relationships for polymer electrodes compared to commercial gold-film electrode arrays and reporter-based endpoint assays. Considering their technical advantages over established assays, impedance readings based on low-cost polymer electrode arrays may become an attractive alternative to conventional assays using luminescent or colorimetric readouts.</p>","PeriodicalId":100109,"journal":{"name":"Applied Research","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/appl.70004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143248773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Open-Source Tools for Assessing Cytoskeleton Properties in Pathological Conditions From Microscopy Images: An Application Note 从显微镜图像评估病理情况下细胞骨架特性的开源工具:应用说明
Applied Research Pub Date : 2025-01-30 DOI: 10.1002/appl.70005
Magdalini Petrou, Andreas Stylianou
{"title":"Open-Source Tools for Assessing Cytoskeleton Properties in Pathological Conditions From Microscopy Images: An Application Note","authors":"Magdalini Petrou,&nbsp;Andreas Stylianou","doi":"10.1002/appl.70005","DOIUrl":"https://doi.org/10.1002/appl.70005","url":null,"abstract":"<p>The cytoskeleton is a multiplex network of different proteins and filaments and is involved in a variety of important functions. Nevertheless, its deregulation leads to various diseases. Cells' cytoskeleton characteristics and structures can be accessed with image analysis tools. Our purpose, therefore, was to identify relevant freeware tools that can be used for assessing cytoskeleton characteristics from microscopy images and create an application note for them. The tools covered in this application note are the ImageJ/Fiji, FilamentSensor, and Ilastik software. These three softwares are presented and described regarding their uses, features and other characteristics, and examples of using this software for analyzing the cell cytoskeleton are provided. These tools might contribute to the advancement of biological research and the enhancement of therapeutic outcomes, particularly regarding pathological conditions, suggesting further development and adoption of open-source tools in the scientific community.</p>","PeriodicalId":100109,"journal":{"name":"Applied Research","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/appl.70005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143120876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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