Applied Research最新文献_第10页

Development of a label-free, impedance-based biosensor to identify harmful effects of pesticides on insect cells 开发无标记、基于阻抗的生物传感器，以识别农药对昆虫细胞的有害影响

Applied Research Pub Date : 2024-05-02 DOI: 10.1002/appl.202400032

Sandra Friedrich, Neha Malagimani, Stefanie Michaelis, Joachim Wegener

{"title":"Development of a label-free, impedance-based biosensor to identify harmful effects of pesticides on insect cells","authors":"Sandra Friedrich, Neha Malagimani, Stefanie Michaelis, Joachim Wegener","doi":"10.1002/appl.202400032","DOIUrl":"10.1002/appl.202400032","url":null,"abstract":"Insects are a major part of the planet's ecosystem and their vital role as pollinators for agriculture is undisputed. Alongside factors as climate change or loss of habitats, rising use of pesticides emerges as a key threat to insect populations. For fighting this man-made problem, development of an easy, fast, sensitive, and non-invasive biosensor for determining pesticide toxicity may help to ban harmful substances and formulations. Here, a biosensor based on Sf21 (Spodoptera frugiperda) insect cells as sensors and electric cell-substrate impedance sensing (ECIS) as physical transducer is described. Sf21 cell suspensions and well-defined pesticide solutions were mixed immediately before seeding on planar gold-film electrodes. The capacitance at 20 kHz was recorded as a function of time as a measurand for cell adhesion providing dose–response profiles of pesticide impact. For future in-field applications, decoupling of the cell culture routines from the actual cytotoxicity assay is mandatory. Thus, suspensions of Sf21 cells were cryopreserved at –80°C in the wells of multielectrode arrays and thawed anytime for conducting the assays. Five pesticides were tested for their concentration-dependent cytotoxicity expressed as EC50 values by ECIS and validated using the well-established WST-1 cell viability assay. Results were found to be in good agreement. Our studies revealed cytotoxic effects of some pesticides sold for home usage far below the recommended concentration and were found to be more toxic than formulations sold for agricultural industry only.","PeriodicalId":100109,"journal":{"name":"Applied Research","volume":"3 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/appl.202400032","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141019321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence emission angular dependence on a nanostructured plasmonic grating 纳米结构质子光栅的荧光发射角度依赖性

Applied Research Pub Date : 2024-04-18 DOI: 10.1002/appl.202300090

Margherita Angelini, Eliana Manobianco, Paola Pellacani, Francesco Floris, Franco Marabelli

引用次数: 0

Laser-assisted graphene-like materials for oil-spill clean up 用于溢油清理的激光辅助石墨烯类材料

Applied Research Pub Date : 2024-04-11 DOI: 10.1002/appl.202300114

Miltiadis Zamparas, Michail Athanasiou, Nikolaos Samartzis, Vassileios Dracopoulos, Spyros Yannopoulos, Theophilos Ioannides

{"title":"Laser-assisted graphene-like materials for oil-spill clean up","authors":"Miltiadis Zamparas, Michail Athanasiou, Nikolaos Samartzis, Vassileios Dracopoulos, Spyros Yannopoulos, Theophilos Ioannides","doi":"10.1002/appl.202300114","DOIUrl":"10.1002/appl.202300114","url":null,"abstract":"Conventional synthetic sorbents for oil spill cleanup are the most widely employed materials, although a major drawback is the extensive chemical modification that they have undergone, making them economically and environmentally nonsustainable. The use of inexpensive, abundant, nontoxic, biodegradable, and reusable lignocellulosic materials might be an alternative to conventional sorbents, with obvious positive impact on sustainability and circular economy. The present study subsequently utilizes the well-established process, of converting grape molasses—a byproduct of the winemaking industry that represents a typical and abundant source of biomass—into a laser-assisted graphene-like product (LA-B). Comparative experiments were carried out with two additional materials that are similarly produced by the same laser-assisted method, yet use synthetic polyimide foil and tape polymers as precursors, namely LA-F and LA-T. The results showed that diesel oil adsorption capacity of LA-B was 33.5 g/g, while the ultraoleophilic LA-T adsorbed 57 g/g and LA-F adsorbed 41 g/g. Furthermore, the crude oil uptake was 31.1, 56.0, and 38.6 g/g for LA-B, LA-T, and LA-F, respectively. The adsorption of oil on the used materials could be well-described by pseudo-second-order kinetics, showing that over 80% of oil was removed from the water within 15 min.","PeriodicalId":100109,"journal":{"name":"Applied Research","volume":"3 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/appl.202300114","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140715215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photocatalytic degradation of methylene blue by anatase TiO2 coating 锐钛型二氧化钛涂层光催化降解亚甲基蓝

Applied Research Pub Date : 2024-04-09 DOI: 10.1002/appl.202300081

Nikolai Desch, Angela Rheindorf, Cornelia Fassbender, Marc Sloot, Markus Lake

{"title":"Photocatalytic degradation of methylene blue by anatase TiO2 coating","authors":"Nikolai Desch, Angela Rheindorf, Cornelia Fassbender, Marc Sloot, Markus Lake","doi":"10.1002/appl.202300081","DOIUrl":"10.1002/appl.202300081","url":null,"abstract":"Photocatalytic coatings have the potential to contribute to the purification of water via an advanced oxidation process (AOP). A commonly used method for analyzing the mechanism of the photocatalytic performance of a given reactor type is to document the degradation behavior in a solution containing methylene blue. However, since methylene blue is rather unstable, the degradation results should be viewed critically. In this work, the degradation behavior of a test solution with methylene blue on quartz glass surfaces coated with photocatalytic titanium dioxide (TiO2) of the anatase modification was investigated through a variety of different light sources. The coating was deposited by physical vapor deposition (PVD) with the reactive pulsed DC magnetron sputtering ion plating (MSIP) method described in the study by Desch and Lake, while the quartz glasses were coated with a 100 nm thick TiO2 coating on the outside. The same glasses were used for all experiments with TiO2. In the determination of the degradation rate, additional experiments were performed using pure quartz glass without any coating, which made it possible to examine the influence of different light sources on the degradation rate of methylene blue in general. Three different light sources, namely UV-A, UV-C, and simple fluorescent lamps were used in this study. The concentration of methylene blue was recorded by photo spectrometer in 10-min increments throughout the experiment and the experiments were performed for 24 h in all cases. Our data indicates that the methylene blue test is a poor method because the degradation rate is not clearly differentiable due to the low stability of the test substance. Without including reference testing in the absence of a catalyst, data may be subject to misinterpretation.","PeriodicalId":100109,"journal":{"name":"Applied Research","volume":"3 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/appl.202300081","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140725908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparing mass transfer and reaction rate kinetics in starch hydrolysis during food digestion 比较食物消化过程中淀粉水解的传质和反应速率动力学

Applied Research Pub Date : 2024-04-09 DOI: 10.1002/appl.202400023

Yongmei Sun, Zelin Zhou, Chao Zhong, Zexin Lei, Timothy A. G. Langrish

{"title":"Comparing mass transfer and reaction rate kinetics in starch hydrolysis during food digestion","authors":"Yongmei Sun, Zelin Zhou, Chao Zhong, Zexin Lei, Timothy A. G. Langrish","doi":"10.1002/appl.202400023","DOIUrl":"10.1002/appl.202400023","url":null,"abstract":"This study demonstrates evidence that the mass transfer process of an enzyme (a biocatalyst) is the rate-limiting step in the starch hydrolysis reaction during food digestion. The significance of this work has been to compare the reaction rate of starch hydrolysis by salivary enzymes with the mass transfer rate of rate-limiting enzymes. This research has applied mass transfer and reaction engineering theory in a quantitative study of starch hydrolysis, and a dimensionless group, the Damköhler number (Da), has been calculated based on glucose measurements from a beaker and stirrer system. The values of the Da number in this study (0.3–19) indicate that both the time constant for mass transfer and the time constant for reaction are significant parameters. Scanning electron microscopy images emphasize that compression (simulated mastication) helps to break the plant cell wall of starch. Mass-transfer resistance needs to be considered during food digestion studies. The Da numbers are significantly affected by both compression forces (internal mass-transfer coefficients) and stirrer speeds (external mass-transfer coefficients) in this beaker and stirrer system.","PeriodicalId":100109,"journal":{"name":"Applied Research","volume":"3 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/appl.202400023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140726452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving performance of aerosol jet printing using machine learning-driven optimization 利用机器学习驱动的优化技术提高气溶胶喷射印刷的性能

Applied Research Pub Date : 2024-04-05 DOI: 10.1002/appl.202300110

Prashantkumar Pandey, Steffen Ziesche, Gauranggiri Meghanathi

{"title":"Improving performance of aerosol jet printing using machine learning-driven optimization","authors":"Prashantkumar Pandey, Steffen Ziesche, Gauranggiri Meghanathi","doi":"10.1002/appl.202300110","DOIUrl":"10.1002/appl.202300110","url":null,"abstract":"Aerosol jet printing is a promising technology for printing functional materials on a variety of substrates with high precision and resolution. This technology has the potential to revolutionize the manufacturing industry by providing a low-cost, high-resolution printing technique that can be used to produce additively printed electronics, sensors, and energy devices. However, the optimization of this process has traditionally relied on time-consuming trial-and-error methods, hampering its efficiency and scalability. Machine learning (ML) models have the potential to overcome these challenges and improve the quality, speed, and efficiency of the printing process. In this paper, we propose an approach that leverages ML algorithms to streamline and enhance the aerosol jet printing optimization process. Our methodology involves data collection through systematic experimentation with various parameter settings. This data set serves as the foundation for training different ML model capable of predicting printed line characteristics and optimal printing process parameter. We validate our approach by performing experiments on different inks, and we compare the results of our ML-based optimization approach to those obtained using traditional trial-and-error methods. The results demonstrate that our approach offers significantly higher accuracy and efficiency. To enhance our approach's accessibility and ease of use, we incorporate AutoML techniques which automates the process of selecting the most suitable ML algorithms and hyperparameters, reducing the burden of manual configuration. Furthermore, we introduce a user-friendly web-based interface that facilitates the entire ML pipeline, from data preprocessing to prediction and batch processing. This interface empowers users to efficiently manage and manipulate their data, select appropriate ML algorithms, and execute predictions, ultimately improving accuracy and model performance.","PeriodicalId":100109,"journal":{"name":"Applied Research","volume":"3 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/appl.202300110","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140738461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integration and characterization of a zeolite material in a microcomponent for measurements of environmental carbon dioxide 用于测量环境二氧化碳的微型组件中沸石材料的集成与表征

Applied Research Pub Date : 2024-04-04 DOI: 10.1002/appl.202300105

Erika Åkerfeldt, Greger Thornell, Anders Persson

{"title":"Integration and characterization of a zeolite material in a microcomponent for measurements of environmental carbon dioxide","authors":"Erika Åkerfeldt, Greger Thornell, Anders Persson","doi":"10.1002/appl.202300105","DOIUrl":"10.1002/appl.202300105","url":null,"abstract":"This study demonstrates integration of a zeolite material in a ceramic microcomponent intended for use in sampling and analysis of environmental carbon dioxide (CO2). The zeolite material was integrated in bulk form, allowing for adsorption of large quantities of CO2 compared to previous integration attempts as thin films. To obtain a porous bulk material, an injectable slurry was developed, where expandable polymeric microspheres were added as a sacrificial template. By varying water and sphere contents of the slurry, it was possible to tune the porosity of the zeolite material between 55% and 72%. This in turn affected the flow resistance of the microcomponents, where an increase in the porosity of the filling from 62% to 72% reduced the flow resistance from 84 to 28 kPa min cm−3. In addition, the spheres facilitated complete fillings free from cracks. The zeolite material was seen to retain its ability to adsorb CO2 after processing, but it was not possible to quantify the level of retention compared to unprocessed zeolite.","PeriodicalId":100109,"journal":{"name":"Applied Research","volume":"3 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/appl.202300105","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140743010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sonification methods for enabling augmented data analysis applied to graphene optoelectronics 应用于石墨烯光电子学的增强数据分析声化方法

Applied Research Pub Date : 2024-04-03 DOI: 10.1002/appl.202300092

Adam Johan Bergren, Angela Beltaos, Alexander van Dijk

引用次数: 0

Cover Image: Volume 3 Issue 2 封面图片：第 3 卷第 2 期

Applied Research Pub Date : 2024-04-02 DOI: 10.1002/appl.202470201

Jianhui Liu, Xiaolin Liu, Tianfu Zhang, Jinqing Huang, Ben Zhong Tang, Ying Chau

引用次数: 0

Effect of carbonization temperatures on the synthesis of biocarbon from Borassus flabellifer fruit fiber for capacitive energy storage 碳化温度对用于电容储能的花叶鲍鱼果纤维生物碳合成的影响

Applied Research Pub Date : 2024-03-30 DOI: 10.1002/appl.202400005

Natarajan Sumangala Devi, Singaravelu Vivekanandhan

{"title":"Effect of carbonization temperatures on the synthesis of biocarbon from Borassus flabellifer fruit fiber for capacitive energy storage","authors":"Natarajan Sumangala Devi, Singaravelu Vivekanandhan","doi":"10.1002/appl.202400005","DOIUrl":"10.1002/appl.202400005","url":null,"abstract":"Borassus flabellifer (Palmyra) fruit fiber (PFF) was extracted and converted into biocarbon materials at different carbonization temperatures of 400°C, 600°C, and 800°C under N2 atmosphere for 1 h. The physicochemical properties of obtained biocarbon materials were investigated via CHNS, SEM-EDX, FTIR, RAMAN, XRD, BET-specific surface area, and TGA techniques. The obtained results were correlated with the capacitive energy storage application of biocarbon materials. The highest specific surface area of 46.36 m2 g−1 was observed for the biocarbon materials obtained at 800°C, which also showed superior capacitive performances. The specific capacitances of PFF-derived biocarbon materials obtained at 400°C, 600°C, and 800°C are respectively found to be 28.58, 37.07, and 45.76 F g−1 at 1 A g−1 employing 1M KOH as electrolyte. The biocarbon materials obtained at 800°C showed the highest specific energy and power of 1.99 and 261 W kg−1 at 1 A g−1.","PeriodicalId":100109,"journal":{"name":"Applied Research","volume":"3 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/appl.202400005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140363416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0