Advances in Sample Preparation最新文献

{"title":"Recent cutting-edge approaches to the integration of solid-liquid extraction with deep eutectic solvents: Toward a greener procedure for biomass valorization","authors":"Niluh Indria Wardani ,&nbsp;Thidarat Samkumpim ,&nbsp;Waleed Alahmad ,&nbsp;Andrew William King ,&nbsp;Pakorn Varanusupakul ,&nbsp;Andrey Shishov ,&nbsp;Noorfatimah Yahaya ,&nbsp;Nur Nadhirah Mohamad Zain","doi":"10.1016/j.sampre.2024.100113","DOIUrl":"https://doi.org/10.1016/j.sampre.2024.100113","url":null,"abstract":"<div><p>As the global demand for sustainable and renewable resources intensifies, there is an imperative to explore innovative technologies for biomass valorization. This review delves into a promising avenue, providing an overview of green approaches that combine solid-liquid extraction (SLE) with a type of solvent known as deep eutectic solvents (DESs) in a synergistic manner. SLE, a conventional method for isolating bioactive compounds from biomass, is recognized for its effectiveness in sample preparation. However, it often involves the use of environmentally harmful solvents. Subsequently, DESs have emerged as an eco-friendly alternative to traditional solvents. Composed of naturally occurring and benign components, these DESs exhibit unique properties that render them suitable for various extraction processes. The integration of SLE with DESs introduces a novel approach to biomass valorization. This review explores the synergistic effects between SLE and DESs to optimize extraction yields, improve selectivity, and reduce overall energy consumption. Furthermore, the nature of DESs aligns with the principles of green chemistry, positioning them as a sustainable alternative to traditional solvents.</p></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"10 ","pages":"Article 100113"},"PeriodicalIF":0.0,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772582024000123/pdfft?md5=dbc23c7eac0f6fedd1940ef8f67b24b3&pid=1-s2.0-S2772582024000123-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140342107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in sample preparation for volatile profiling of plant proteins: Fundamentals and future perspectives","authors":"Wellington da Silva Oliveira,&nbsp;Igor Shepelev,&nbsp;Fernanda F.G. Dias,&nbsp;Gary A. Reineccius","doi":"10.1016/j.sampre.2024.100111","DOIUrl":"10.1016/j.sampre.2024.100111","url":null,"abstract":"<div><p>Due to the increasing world population and consumer desire for sustainable and nutritious protein sources, the food industry is currently experiencing tremendous pressure to develop innovative, sensorially acceptable, health-promoting plant-based products. Plant proteins, specifically pulse proteins arise as a healthy and sustainable protein source for food applications. However, the use of pulse protein is challenged by its intrinsic undesirable aroma often described as beany, grassy, and green. These off notes can be inherent, i.e. generated from plant metabolism, or produced during processing and/or storage and encompass a broad variety of chemical classes. Since aroma plays a major role in consumer acceptance and eating habits and due to its chemical complexity, proper analytical methods must be used for its assessment. Considering that the effectiveness of an analytical method is significantly influenced by its sample preparation step, this review focuses on sample preparation techniques for monitoring the volatile profile of pulse proteins, their advantages, and disadvantages, while concurrently highlighting the potential of novel technologies for advancing this critical application.</p></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"10 ","pages":"Article 100111"},"PeriodicalIF":0.0,"publicationDate":"2024-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S277258202400010X/pdfft?md5=f42c49068959ead6a21a9b61a55853f7&pid=1-s2.0-S277258202400010X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140271118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Dry-herb vaporizer-assisted solid-phase Microextraction for the analysis of volatile Organic compounds","authors":"A K M Ahsan Ahmed,&nbsp;Douglas E. Raynie","doi":"10.1016/j.sampre.2024.100112","DOIUrl":"https://doi.org/10.1016/j.sampre.2024.100112","url":null,"abstract":"<div><p>Solid-phase microextraction (SPME) is a simple, efficient, and solvent-free technique. It integrates the steps of traditional liquid-liquid extraction, i.e., sampling, extraction, concentration, derivatization, in a single step. It is easy, combining capability with gas chromatography (GC), liquid chromatography (LC), and mass spectrometry (MS), which makes this method fast, reliable, and sensitive. SPME method is often associated with extraction at elevated temperature, since increasing temperature may positively affect extraction by increasing the analyte volatility, diffusion, and extraction rate. In this work, a dry-herb vaporizer (vaping pen) was used as a sample heating device for direct desorption of volatile analytes into the headspace of the device. The extraction was performed by sorbing analytes from their gas phase in the headspace of the vape onto a SPME fiber followed by desorption in the injection port for GC–MS characterization. Compounds from horseradish, cinnamon, and gasoline-spiked soil samples were analyzed. The samples were also analyzed by conventional headspace SPME-GC–MS. The results from both methods were compared. The methods produce comparable results in terms of extracted compounds and relative area percentages, however, the dry-herb vaporizer had improved sensitivity.</p></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"10 ","pages":"Article 100112"},"PeriodicalIF":0.0,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772582024000111/pdfft?md5=0c98a404a2a7657445ab73f884a14fb5&pid=1-s2.0-S2772582024000111-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140163657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing the potentialities of an easy-to-use sample treatment strategy: Multivariate investigation on “Moka extraction” of typical ingredients from dietary supplements","authors":"Matteo Baglietto,&nbsp;Barbara Benedetti,&nbsp;Marina Di Carro,&nbsp;Emanuele Magi","doi":"10.1016/j.sampre.2024.100110","DOIUrl":"https://doi.org/10.1016/j.sampre.2024.100110","url":null,"abstract":"<div><p>Mr. Bialetti invented Moka in 1933 and it still represents the most common way to prepare coffee at home. The process through which Mokas extract components from the ground coffee is a solid-liquid extraction which occurs at high pressure and temperature. These features are desirable in simple sample treatment strategies, since they allow good extraction efficiencies in a short time. Herein, for the first time, Moka-pot extraction was considered as an alternative processing protocol to extract polar compounds from dietary supplements. The effect of four experimental variables on extraction efficiency was evaluated through a fractional factorial design of experiments applied to a pooled matrix. In particular, solvent pH and its content of organic modifier, heating temperature and sample mass (reflecting the ratio to the amount of solvent which has to be kept fixed due to practical needs) were considered. The performances of the best conditions were then validated by determining recoveries (between 52 and 134 %, except for acetylsalicylic acid) and matrix effects (resulting always negligible or moderate at 100-fold dilution) of a spiked matrix which did not present any of the target analytes. They were finally applied to real samples, allowing to quantify some compounds, including artificial sweeteners, methylxanthines and taurine. Results were then compared with the quantities declared on the labels and those obtained with a Salt-Assisted Liquid-Liquid Extraction (SALLE), previously developed. Interestingly, the two methods were comparable for most compounds, but Moka extraction allowed to quantify taurine, which was not recovered with the SALLE. This promising result encourages further work to extend the use of the simple Moka device to other analytes and further matrices.</p></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"10 ","pages":"Article 100110"},"PeriodicalIF":0.0,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772582024000093/pdfft?md5=6e6e252f8609bf70fc14f3ca6793fccd&pid=1-s2.0-S2772582024000093-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140134961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of thermal treatment on quality of biosimilar and originator monoclonal antibodies","authors":"Yiğit Erdemgil ,&nbsp;Merve Çelik Yamacı ,&nbsp;Ceren Pamukcu ,&nbsp;Fulya Ünalp ,&nbsp;Zeynep Zülfiye Yıldırım Keleş ,&nbsp;Ahmet Emin Atik ,&nbsp;Muhittin Abdulkadir Serdar","doi":"10.1016/j.sampre.2024.100109","DOIUrl":"https://doi.org/10.1016/j.sampre.2024.100109","url":null,"abstract":"<div><p>Investigating products under stress conditions provides valuable information for assessing stability, biosimilarity, and degradation behaviors during monoclonal antibody (mAb) development. Proper sample preparation is crucial for accurately evaluating the biosimilarity and effects of stress conditions in comparability assessment, where these studies guide biosimilar mAb development steps. Enzymatic and chemical treatments applied during sample preparation of mAbs generally require treatment of samples in temperatures higher than the storage temperatures of antibodies.</p><p>In this study, samples of a TNF-α inhibitor IgG1 biosimilar (BIO) and its originator (OR) were treated for 7 days at commonly used temperatures during sample preparation. Alterations in the intact IgG, size variants, charge variants, binding kinetics, and post-translational modifications (PTMs) were investigated with CE-SDS, SE-UPLC, icIEF, SPR, and LC-MS/MS, respectively. Samples treated at 50 °C exhibited significant degradation, while minor differences were observed in samples treated at 37 °C. Monomer and intact IgG levels were decreased to levels below 97 % and 94 %, respectively, after 7 days of thermal treatment at 50 °C for both BIO and OR samples. Similar rates of degradation were observed between the treated biosimilar and originator samples. The percent monomer degradation rate between the biosimilar and the originators was similar at 50 °C (<em>p</em> = 0.32). Thermal treatment increased acidic variant levels in the products of the BIO (23.10 %) and OR (23.16 %). During post-translational modification monitoring, an increase in pyroglutamic acid formation and a decrease in C-terminal lysine were observed after thermal treatments. Acidic variant alterations were associated with asparagine deamidation and N-terminal pyroglutamic acid formation. Post-translational modifications were mainly located at the Fc domain, with methionine oxidation and asparagine deamidation as the main modifications occurring at the Fab domain.</p><p>In conclusion, these results revealed that prolonged thermal treatment under elevated temperatures induces molecular alterations, thereby facilitating the degradation of IgG1. In addition, our findings indicate that both BIO and OR lots exhibit similar degradation profiles when subjected to thermal treatments.</p></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"10 ","pages":"Article 100109"},"PeriodicalIF":0.0,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772582024000081/pdfft?md5=f2fff2cf782d7b7e6bb834cc47657b18&pid=1-s2.0-S2772582024000081-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140141792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbon nanotubes in high internal phase emulsion polymer composite for packed-cartridge micro-solid-phase extraction of fluoroquinolones in urine","authors":"Francesca Merlo ,&nbsp;Francesca Colucci ,&nbsp;Giulia De Soricellis ,&nbsp;Francesca Rinaldi ,&nbsp;Enrica Calleri ,&nbsp;Antonella Profumo ,&nbsp;Andrea Speltini","doi":"10.1016/j.sampre.2024.100103","DOIUrl":"10.1016/j.sampre.2024.100103","url":null,"abstract":"<div><p>Aim of this study stands in the evaluation of a carbon nanotubes-based polymerized high internal phase emulsion composite (CNT/polyHIPE) as sorptive phase in biological sample preparation. A micro-solid-phase extraction procedure (µSPE) was developed for trace fluoroquinolone (FQ) antimicrobials, namely Ciprofloxacin, Levofloxacin, Lomefloxacin, Moxifloxacin, Norfloxacin and Sparfloxacin, in human urine samples. PolyHIPEs modified by incorporation of different concentrations of nanotubes (0.2–0.8 wt %) were one-pot synthesized and applied in the packed-cartridge setting. The sorption affinity for the drugs was investigated in tap water and buffered aqueous solutions, demonstrating the key role of the nanotubes embedded in the polymer. The 0.5 wt % CNT composite was selected to develop a straightforward µSPE procedure directly in raw urine (1 mL sample), followed by HPLC-MS/MS. Targets were retained on the sorbent at near neutral pH and, after an aqueous washing (0.1 % v/v formic acid), eluted in a single-step with 4 % v/v ammonia aqueous solution (15 % v/v acetonitrile), thus combining extraction and clean-up. The method allowed accurate quantification of FQs, as evidenced by the recoveries (74–116 %, <em>n</em> <em>=</em> <em>3</em>) obtained on blank pooled urine samples spiked with 40, 75, 150 µg L⁻¹, accompanied by good inter-day precision (RSD &lt; 14 %, <em>n</em> <em>=</em> <em>3</em>). To confirm the applicability of the analytical method, some real-life blind samples were processed as a proof of concept.</p></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"9 ","pages":"Article 100103"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772582024000020/pdfft?md5=98be2f4f951125d5a73daa5c783e09ed&pid=1-s2.0-S2772582024000020-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139632388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multivariate optimization, in conjugation with matrix solid-phase dispersion and digital image detection, allows residual dye determination from shrimp samples","authors":"Jéssica Passos de Carvalho ,&nbsp;Iare Soares Ribeiro ,&nbsp;Marcos Vinícius de Sousa Pereira ,&nbsp;Tatianny de Araujo Andrade ,&nbsp;Jemmyson Romário de Jesus","doi":"10.1016/j.sampre.2024.100105","DOIUrl":"https://doi.org/10.1016/j.sampre.2024.100105","url":null,"abstract":"<div><p>Here, we report an optimization of the extraction of crystal violet and congo red dyes from shrimp samples, employing matrix solid phase dispersion and improved multivariate optimization. Three factors were evaluated: (i) adsorbent nature (neutral alumina, silica gel and C₁₈); (ii) eluent strength (dimethylformamide, methanol and acetonitrile); and (iii) sample-adsorbent ratio (1:1; 1:5; and 1:10, m/m). Successful extraction of dyes was achieved using dimethylformamide as eluent, neutral alumina as adsorbent and sample:adsorbent ratio of 1:5 (m/m). Adsorption and kinetic studies suggest strong interaction between dye and adsorbent. Furthermore, employing digital image colorimetric detection, the study observed limit of detection (LOD) ranging between 3.8 to 29.8 µg <em>L</em> ⁻ ¹ and limit of quantification (LOQ) ranging between 12.3 to 90.3 µg <em>L</em> ⁻ ¹ with R² &gt; 0.9927 for both dyes, CV and CR, respectively. Notably, recovery was greater than 86 % with a relative standard deviation (RSD) inferior to 7 % (<em>n</em> = 3). These findings indicate the effectiveness of the improved multivariate optimization technique in conjunction with matrix solid-phase dispersion for successful extraction and quantification of dyes from shrimp samples, showing its potential for accurate and sensitive detection in similar analyses. The proposed method was compared with the gold method (UV/Vis spectroscopy) and the statistical treatment showed that the results of both methods are comparable.</p></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"9 ","pages":"Article 100105"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772582024000044/pdfft?md5=4e59eb26368687cab5089e61463b286f&pid=1-s2.0-S2772582024000044-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139743487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electromembrane extraction of peptides – Looking retrospectively into published studies","authors":"Torstein Kige Rye ,&nbsp;Samira Dowlatshah ,&nbsp;Frederik André Hansen ,&nbsp;Trine Grønhaug Halvorsen ,&nbsp;Stig Pedersen-Bjergaard","doi":"10.1016/j.sampre.2024.100106","DOIUrl":"https://doi.org/10.1016/j.sampre.2024.100106","url":null,"abstract":"<div><p>Electromembrane extraction (EME) has been developed into an attractive microextraction concept for extraction of pharmaceuticals and related substances from blood and urine. In EME, charged substances are extracted across a liquid membrane (organic) under the influence of an electrical field. Recently, commercial equipment for EME was launched, and this is expected to increase the interest for the technique. EME has also been explored for extraction of peptides, but this is more complicated due to the challenges related to the solvation of peptides in the organic liquid membrane. In this paper, we review the literature on EME of peptides, and we retrospectively look closer into published studies based on our current understanding of the concept. By such, we can explain early observations in more detail, and we can improve our fundamental understanding.</p></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"9 ","pages":"Article 100106"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772582024000056/pdfft?md5=c4fae8cfacb8433d1a168c32dcffc955&pid=1-s2.0-S2772582024000056-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139935348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent applications of tetramethylammonium hydroxide (TMAH) in the sample preparation for elemental analysis by spectroanalytical techniques","authors":"Uillian Mozart Ferreira Mata Cerqueira ,&nbsp;Juscelia Pereira Santos Alves ,&nbsp;Walter Nei Lopes Santos ,&nbsp;Bernardo da Silva Pita ,&nbsp;Cleber Galvão Novaes ,&nbsp;Sulene Alves Araújo ,&nbsp;Marcos Almeida Bezerra","doi":"10.1016/j.sampre.2024.100104","DOIUrl":"https://doi.org/10.1016/j.sampre.2024.100104","url":null,"abstract":"<div><p>Application of tetramethylammonium hydroxide for the treatment of samples prior to elemental analysis over the last five years is approached in this review article. Characteristics and properties of this reagent are described for a better understanding of the treatment process. Its effect on analyte measurements performed by atomic spectrometry techniques (flame and graphite furnace atomic absorption spectrometry, atomic fluorescence spectrometry, inductively coupled plasma optical emission spectrometry and, inductively coupled plasma mass spectrometry) is discussed, mainly the possibilities of physical interferences. Ways of using the reagent in sample preparation, such as incubation at room temperature, use of ultrasound energy, microwave energy, among others, will be discussed, in addition to the use of the reagent in speciation and nanoparticles analyses. Tetramethylammonium hydroxide has proven to be a viable alternative in the treatment of samples that can improve the performance of methods based on atomic spectrometry in the determination of elements of great interest to society.</p></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"9 ","pages":"Article 100104"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772582024000032/pdfft?md5=5528d409ff4e2be11b99436543eef01a&pid=1-s2.0-S2772582024000032-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139727172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salt-assisted liquid-liquid extraction and on-column concentration for chromatographic determination of phenolic compounds in beer","authors":"Luís Claudio Martins ,&nbsp;Maria Soledad M.S.F. Acevedo ,&nbsp;Mariana R. Gama ,&nbsp;Fábio R.P. Rocha","doi":"10.1016/j.sampre.2024.100107","DOIUrl":"https://doi.org/10.1016/j.sampre.2024.100107","url":null,"abstract":"<div><p>Salt-assisted liquid-liquid extraction (SALLE) and on-column concentration were evaluated to simplify the chromatographic determination of phenolic compounds in beer. Resveratrol, flavonoids (quercetin and kaempferol), and phenolic acids (caffeic, p-coumaric, and trans-ferulic acids) were used as model compounds based on their concentrations reported in craft beers. SALLE with acetonitrile was effective for sample cleanup, with recoveries ranging from 84% to 112%. The on-column concentration was carried out in high performance liquid chromatography by starting the gradient elution with acidified water (sample carrier stream) in a reversed-phase mode. Consequently, the analytes were retained in a narrow band, and highly polar/ionic potentially interfering species were flushed from the column. The effectiveness of the approach was demonstrated by linear correlations between the peak areas and the injected volumes from 10 to 200 µL (r ≥ 0.999), without significant variations in peak width (&lt; 10%) or retention times (&lt; 0.7%), and peak symmetry (tailing factors within the range of 0.8–1.1). For all analytes, a linear response was achieved from 100 to 1000 µg L⁻¹ (r &gt; 0.999), with limits of detection within the range of 9–82 µg L⁻¹. The approach was also effective for on-column concentrations of resveratrol, quercetin, and kaempferol in sequential injection chromatography (SIC), with injected volumes up to 750 µL and linear responses within 100–1000 µg L⁻¹ (r &gt; 0.997). The proposal reduced consumption of organic solvents (with only 770 µL required per determination in SIC) and avoided solid-phase extraction cartridges for analyte preconcentration.</p></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"9 ","pages":"Article 100107"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772582024000068/pdfft?md5=49c7880fd7ad0dceefdb9e793f273e9b&pid=1-s2.0-S2772582024000068-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139986405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}