Advances in Sample Preparation最新文献_第5页

New trends in bioanalysis sampling and pretreatment: How modern microsampling is revolutionising the field 生物分析采样和预处理的新趋势：现代微采样如何彻底改变该领域

IF 5.2

Advances in Sample Preparation Pub Date : 2025-02-01 DOI: 10.1016/j.sampre.2025.100161

Michele Protti , Elisa Milandri , Roberta Di Lecce , Laura Mercolini , Roberto Mandrioli

{"title":"New trends in bioanalysis sampling and pretreatment: How modern microsampling is revolutionising the field","authors":"Michele Protti , Elisa Milandri , Roberta Di Lecce , Laura Mercolini , Roberto Mandrioli","doi":"10.1016/j.sampre.2025.100161","DOIUrl":"10.1016/j.sampre.2025.100161","url":null,"abstract":"<div><div>Microsampling technologies are revolutionising bioanalysis by enabling minimally invasive, decentralized sample collection, offering significant advantages over traditional methods in terms of patient compliance, cost-effectiveness, and analytical efficiency. This review explores the latest advancements in microsampling devices, including microfluidic and quantitative dried blood spot systems (mfDBS and qDBS, respectively), calibrated capillary-based devices, volumetric absorptive microsampling (VAMS) technologies, microneedle-based microsampling, radial-based DBS devices, membrane-based plasma separation technologies and vacuum-assisted blood collection systems. By addressing pre-analytical variability, enhancing analyte stability, and supporting decentralized workflows, these technologies align with modern and green analytical chemistry principles. The unique capabilities of these emerging microsampling technologies and devices further demonstrate their transformative potential. However, challenges remain, including haematocrit dependency for some parameters, user variability, and standardisation across devices. This review highlights ongoing innovations and their implications for expanding to high-quality bioanalytical testing.</div></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"13 ","pages":"Article 100161"},"PeriodicalIF":5.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143512198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of an automated sorptive extraction system for trace analysis in water 用于水中痕量分析的自动吸附萃取系统的研制

IF 5.2

Advances in Sample Preparation Pub Date : 2025-02-01 DOI: 10.1016/j.sampre.2025.100156

Frank Jacobs , Dr. Klaus Kerpen , Dr. Eike Kleine-Benne , PD Dr. Ursula Telgheder

{"title":"Development of an automated sorptive extraction system for trace analysis in water","authors":"Frank Jacobs , Dr. Klaus Kerpen , Dr. Eike Kleine-Benne , PD Dr. Ursula Telgheder","doi":"10.1016/j.sampre.2025.100156","DOIUrl":"10.1016/j.sampre.2025.100156","url":null,"abstract":"<div><div>Stir bar sorptive extraction (SBSE) is an established microextraction technique developed in the early 2000s. It has gained widespread use in laboratories around the world, but always had one drawback in that it was a manual sample preparation technique. The newly developed system presented in this study aims to combine the well-known characteristics of SBSE with fully automated sample preparation. The presented system is based on a novel sample probe design, which uses the same dimensions and materials of sorbent phase as the commercially available Gerstel Twister® SBSE technology. To test the performance of this system, pesticides were extracted from pure water as well as river water. Limits of detection (4.5 ng/L to 53.6 ng/L) and quantification (15.7 ng/L to 327.6 ng/L) as well as standard method deviation (3.2 % to 37.6 %) were determined for 21 pesticides based on DIN EN ISO 27,108, part of the water framework directive (WFR). Standard addition was used to quantify pesticides in river water matrix. To check the performance of the new development, it was compared to established methods like manual SBSE, thin film micro extraction (TFME) and SPME-Arrow. The results are comparable between automated sorptive extraction (ASE) and SBSE (average of 84 % compared to automated system), as expected. Performance compared to TFME (65 %) and SPME-Arrow (3 %) was significantly better, especially to the latter. These results show that the automated sorptive extraction system developed in this paper is competitive with already established techniques.</div></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"13 ","pages":"Article 100156"},"PeriodicalIF":5.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143527262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vial-coated thin film microextraction for perfluoroalkyl carboxylic acids (PFCAs) determination in water by GC–MS 气相色谱-质谱法测定水中全氟烷基羧酸（PFCAs）

IF 5.2

Advances in Sample Preparation Pub Date : 2025-02-01 DOI: 10.1016/j.sampre.2025.100147

J․Mabel Luna-Díaz , Luz O․ Leal-Quezada , Laura Ferrer , Edwin Palacio

{"title":"Vial-coated thin film microextraction for perfluoroalkyl carboxylic acids (PFCAs) determination in water by GC–MS","authors":"J․Mabel Luna-Díaz , Luz O․ Leal-Quezada , Laura Ferrer , Edwin Palacio","doi":"10.1016/j.sampre.2025.100147","DOIUrl":"10.1016/j.sampre.2025.100147","url":null,"abstract":"<div><div>Perfluoroalkyl carboxylic acids (PFCAs), a subclass of perfluoroalkyl substances (PFAs) commonly detected in water, are of increasing concern due to their persistence, bioaccumulative nature, and harmful effects on health. Determining PFCAs in environmental matrices is challenging because they are typically present at ng L⁻¹ levels and tend to adsorb onto materials like filtration membranes, causing significant losses during analysis. To address these challenges, a thin-film microextraction (TFME)-coated vial was designed for PFCA (C5–C10) extraction, coupled with gas chromatography-mass spectrometry (GC–MS). Glass was selected as the support material due to its low adsorption rate and superior chemical resistance compared to other polymers. The TFME coating, made from a weak anion exchange resin and polyacrylonitrile (10 % w/w) as a binder polymer, effectively extracts analytes from complex matrices while minimizing co-extraction of interfering macromolecules. The method eliminates the need for sample filtration, preventing PFCA loss in filtration membranes. Under optimal conditions, the extraction efficiency ranged from 70 to 100 %. Detection and quantification limits were 0.13–2.1 ng L⁻¹ and 0.42–7.1 ng L⁻¹, respectively. Intra- and inter-assay relative standard deviations (% RSD) were 4.9–2.2 % and 4.8–2.3 %, respectively, with a linear working range of 10–1000 ng L⁻¹ for the TFME-coated device. This method is suitable for PFCA determination in drinking water and complies with the Directive 2020/2184, which sets a maximum allowable limit of 500 ng L⁻¹ for total PFAs.</div></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"13 ","pages":"Article 100147"},"PeriodicalIF":5.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143130333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrospun chitosan modified montmorillonite/polyvinyl alcohol nanofibers as suitable coating for fiber-in-tube solid-phase microextraction of trace amounts of tricyclic antidepressants in biological fluids 静电纺壳聚糖改性蒙脱土/聚乙烯醇纳米纤维涂层用于管中纤维固相微萃取生物体液中微量三环类抗抑郁药

IF 5.2

Advances in Sample Preparation Pub Date : 2025-02-01 DOI: 10.1016/j.sampre.2024.100145

Saeed Pourhosseini, Yadollah Yamini, Seyedeh Sara Nasrollahi

{"title":"Electrospun chitosan modified montmorillonite/polyvinyl alcohol nanofibers as suitable coating for fiber-in-tube solid-phase microextraction of trace amounts of tricyclic antidepressants in biological fluids","authors":"Saeed Pourhosseini, Yadollah Yamini, Seyedeh Sara Nasrollahi","doi":"10.1016/j.sampre.2024.100145","DOIUrl":"10.1016/j.sampre.2024.100145","url":null,"abstract":"<div><div>Chitosan intercalated montmorillonite and polyvinyl alcohol-citric acid (PVA/CA) nanofibers were electrospun onto stainless steel wires. The fibers were placed inside a stainless steel tube and utilized for fiber-in-tube solid phase microextraction (FIT-SPME) to detect trace levels of tricyclic antidepressant drugs (TCAs) in biological samples. The analytes were separated and quantified using gas chromatography coupled with mass spectrometric detection (GC–MS). The effects of various parameters on the extraction efficiency, including pH (9), extraction time (25 min), extraction flow rate (3 mL min<sup>-1</sup>), salt addition (0 % w/v), type of eluent (ethanol 99 %), eluent volume (300 µL), and desorption flow rate (70 µL min<sup>-1</sup>), were investigated and optimized. Under optimal conditions, the linear dynamic range for doxepin, and sertraline was found to be 0.08–750.0 ng mL<sup>-1</sup>; for amitriptyline, desipramine, and trimipramine, it was 10.0–750.0 ng mL<sup>-1</sup>; and for carbamazepine and maprotiline, it was 50.0–750.0 ng mL<sup>-1</sup>. The proposed method exhibited low limits of detections in the range of 0.02–15.2 ng mL<sup>-1</sup>. Additionally, coefficients of determination (R²) exceeding 0.9911 were achieved, indicating excellent linearity for the calibration curves. To assess the feasibility of the FIT-SPME-GC–MS method, extraction and analysis of TCAs were performed on plasma and urine samples. Relative recovery percentages (RR%) ranged from 90.3 % to 117.6 %.</div></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"13 ","pages":"Article 100145"},"PeriodicalIF":5.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143131051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards eco-metabolomics: NADES-guided extraction enables semi-quantitative metabolomics for Melissa officinalis 走向生态代谢组学：nades引导提取实现了药用草的半定量代谢组学

IF 5.2

Advances in Sample Preparation Pub Date : 2025-02-01 DOI: 10.1016/j.sampre.2025.100154

Chiara Spaggiari , Isa Sara Aimee Hiemstra , Antoinette Kazbar , Gabriele Costantino , Laura Righetti

{"title":"Towards eco-metabolomics: NADES-guided extraction enables semi-quantitative metabolomics for Melissa officinalis","authors":"Chiara Spaggiari , Isa Sara Aimee Hiemstra , Antoinette Kazbar , Gabriele Costantino , Laura Righetti","doi":"10.1016/j.sampre.2025.100154","DOIUrl":"10.1016/j.sampre.2025.100154","url":null,"abstract":"<div><div>In recent years, there has been a growing emphasis on the development of green extraction techniques that minimize environmental impact while maximizing yield of the extracted compounds. To this aim, in this study we investigated the potential of green solvents for extracting bioactive compounds from <em>Melissa officinalis</em> (MO) leaves. Specifically, we focus on the application of 20 Natural Deep Eutectic Solvents (NADES) with a relative polarity ranging from 0.34 to 1.29. Their extraction affinity against a set of 11 plant metabolites was predicted using COSMO-RS software and experimentally validated using quantitative LC<img>HRMS analysis. Subsequently, the same extracts were subjected to non-target metabolomics to uncover the NADES selectivity towards the wide spectrum of MO leaf metabolites. Data preprocessing and feature alignment were performed using MZmine, and aligned features were annotated using SIRIUS+CSI:FingerID.</div><div>Overall, 249 and 195, metabolites were annotated in positive and negative ionization ion mode, respectively. Additionally, to have a more accurate view of the different NADES extraction capacity, we adopted a semi-quantitative approach that enables the prediction of concentration for all the annotated metabolites (N = 444).</div><div>The results highlighted the selectivity of some NADES in extracting very diverse biochemical classes, providing valuable insights into the composition and concentration of bioactive compounds. Interestingly, thymol-menthol NADES demonstrated the ability to efficiently extract a broad range of bioactive compounds, yielding a metabolome comparable to that obtained with conventional ethanolic. Overall, the entire workflow facilitated the green extraction and annotation of known bioactive molecules that had never been described in MO.</div></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"13 ","pages":"Article 100154"},"PeriodicalIF":5.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodiesel as a green alternative solvent in dispersive liquid-liquid microextraction 生物柴油作为分散液-液微萃取的绿色替代溶剂

IF 5.2

Advances in Sample Preparation Pub Date : 2025-02-01 DOI: 10.1016/j.sampre.2025.100163

Samara Soares, Fábio R.P. Rocha

{"title":"Biodiesel as a green alternative solvent in dispersive liquid-liquid microextraction","authors":"Samara Soares, Fábio R.P. Rocha","doi":"10.1016/j.sampre.2025.100163","DOIUrl":"10.1016/j.sampre.2025.100163","url":null,"abstract":"<div><div>Biodiesel, a fuel derived from renewable sources, is a nonpolar liquid, composed of alkyl esters derived from long-chain carboxylic acids. Because of its environmental friendliness, large-scale production, and cost-effectiveness, biodiesel is herein proposed as a green solvent for liquid-liquid microextraction. Its potential has been demonstrated through the extraction of model analytes, with varying polarities through analytical measurements performed by smartphone-based digital images. Partition coefficients biodiesel/water (log P) were –0.45 (pH 2.0) for crystal violet, –1.3 (pH 7.0) for bromocresol green, 1.05 for PAN, 3.48 for Cu(II)-PAN complex, and 2.14 for quercetin. Biodiesel was effective in extracting nonpolar species, with extraction efficiencies of 90 % for PAN, 99 % for quercetin and Cu(II)-PAN complex. Efficiency of extraction of the Cu(II)-PAN complex increased in the following order: chloroform, ethyl acetate, and biodiesel, and the highest recoveries were achieved with biodiesel as solvent. Only slightly variations in the extraction efficiency was observed from biodiesel produced from different feedstock. Biodiesel was also efficient for extraction of the ion-pairs from anionic surfactants and methylene blue, with the perspective of replacing chloroform in this classical method. The results showed that biodiesel is an efficient alternative solvent to replace typical toxic solvents, with greenness demonstrated through the Green Star and AGREE metrics.</div></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"13 ","pages":"Article 100163"},"PeriodicalIF":5.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143518978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tailoring a MOF-on-MOF (ZIF-8-on-NH2-MIL-53(Al)) Hybrid Composite for Micro Solid Phase Extraction of Furosemide in artificial biological fluids prior to LC-q-TOF/MS detection 定制MOF-on-MOF （ZIF-8-on-NH2-MIL-53(Al)）混合复合材料，用于LC-q-TOF/MS检测前人工生物液中速尿的微固相萃取

IF 5.2

Advances in Sample Preparation Pub Date : 2025-02-01 DOI: 10.1016/j.sampre.2025.100157

Zinar Pinar Gumus , Mustafa Soylak

{"title":"Tailoring a MOF-on-MOF (ZIF-8-on-NH2-MIL-53(Al)) Hybrid Composite for Micro Solid Phase Extraction of Furosemide in artificial biological fluids prior to LC-q-TOF/MS detection","authors":"Zinar Pinar Gumus , Mustafa Soylak","doi":"10.1016/j.sampre.2025.100157","DOIUrl":"10.1016/j.sampre.2025.100157","url":null,"abstract":"<div><div>Furosemide, a powerful diuretic that increases urine excretion, is used to treat many diseases. However, due to its abuse in sport, it is considered a doping substance. As a result, it has been banned by the World Anti-Doping Agency (WADA) and included in the group of diuretics and masking agents. Given the clinical significance of furosemide and its dependable application in athletics, this research employed the \"MOF-on-MOF\" hybrid material composite to leverage the synergistic effects of various components for detecting this drug in biological samples. After characterization of the ZIF-8-on-NH<sub>2</sub>-MIL-53(Al) composite by FT-IR, XRD, TGA, XPS, BET and SEM-EDX techniques, the µ-SPE procedure was optimized using liquid chromatography-time of flight mass spectrometry (LC-q-TOF/MS) based on the recovery values. The optimum conditions were determined as pH 6, adsorbent amount of 20 mg, and total adsorption-desorption time of 7 min. The recovery values of the solid-phase extraction method for simulated body fluids samples under optimum conditions were between 77.5 % and 93.4 %. The µ-SPE technique was effectively utilized on physiological samples using the ZIF-8-on-NH<sub>2</sub>-MIL-53(Al) composite under optimal conditions. This study's findings will inform future research into the design of 'MOF-on-MOF' hybrids for various applications.</div></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"13 ","pages":"Article 100157"},"PeriodicalIF":5.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143438238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endogenous HiBiT-tagging combined with affinity complementation: A new strategy for small open reading frame-encoded polypeptide detection in bacteria 内源性hibit标记结合亲和互补：细菌小片段开放阅读框编码多肽检测的新策略

IF 5.2

Advances in Sample Preparation Pub Date : 2025-02-01 DOI: 10.1016/j.sampre.2024.100143

Laure Simoens, Veronique Jonckheere, Dominiek Catteeuw, Petra Van Damme

{"title":"Endogenous HiBiT-tagging combined with affinity complementation: A new strategy for small open reading frame-encoded polypeptide detection in bacteria","authors":"Laure Simoens, Veronique Jonckheere, Dominiek Catteeuw, Petra Van Damme","doi":"10.1016/j.sampre.2024.100143","DOIUrl":"10.1016/j.sampre.2024.100143","url":null,"abstract":"<div><div>Bacterial genome annotations are continuously refined with the advent of novel techniques. Ribosome profiling, or Ribo-seq, utilizing next-generation sequencing to link genomic regions with translational activity, has uncovered numerous small open reading frames (sORFs) - arbitrarily defined as ORFs no longer than 300 base pairs - as a generally under-annotated class of genomic elements in both eukaryotic and prokaryotic genomes. While sORFs can function as regulatory elements, they may also translate into small proteins (equal to or shorter than 100 amino acids), classified as sORF-encoded polypeptides (SEPs). The inherent limitations of ribosome profiling necessitate the experimental validation of predicted (s)ORFs at the protein expression level. However, the small size and unique biochemical characteristics of SEPs pose significant challenges for traditional protein detection methods, like mass spectrometry and immunoblotting. In this study, we introduce HiBiT blotting, a luminescent, complementation-based protein detection method, as a highly sensitive alternative to antibody-based immunoblotting for investigating SEP expression in <em>Salmonella enterica</em> (serovar) Typhimurium (<em>S.</em> Typhimurium) at endogenous levels. We demonstrate its complementarity to mass spectrometry as an expression validation tool. Additionally, employing a biochemical fractionation approach, we determined the subcellular localization of validated <em>S.</em> Typhimurium SEPs, initiating exploration into the functional aspects of these SEPs, and proposed enrichment strategies that may facilitate future experimental validation of Ribo-seq-predicted sORFs.</div></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"13 ","pages":"Article 100143"},"PeriodicalIF":5.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143130330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mixed matrix membranes based on cellulose acetate recycled from cigarette butts and metal-organic frameworks for thin film solid-phase microextraction: Determination of phenols in environmental waters 基于从烟头回收的醋酸纤维素和金属有机骨架的混合基质薄膜固相微萃取：环境水中酚类物质的测定

IF 5.2

Advances in Sample Preparation Pub Date : 2025-02-01 DOI: 10.1016/j.sampre.2025.100150

Massimo G. De Cesaris , María J. Trujillo-Rodríguez , Jorge Pasán , Alessandra Gentili , Verónica Pino

{"title":"Mixed matrix membranes based on cellulose acetate recycled from cigarette butts and metal-organic frameworks for thin film solid-phase microextraction: Determination of phenols in environmental waters","authors":"Massimo G. De Cesaris , María J. Trujillo-Rodríguez , Jorge Pasán , Alessandra Gentili , Verónica Pino","doi":"10.1016/j.sampre.2025.100150","DOIUrl":"10.1016/j.sampre.2025.100150","url":null,"abstract":"<div><div>The recovery of raw materials from waste is a viable strategy to transition to more circular and greener methodologies. In this study, cigarette filters were recycled to obtain cellulose acetate and re-used as a support for a sorbent material in a microextraction procedure. Thus, this biosorbent was combined with a metal-organic framework (MIL-101(<em>Fe</em>)) and configurated in a membrane format of 300 µm of thickness via solvent casting. The developed mixed matrix membranes were characterized by Fourier-transform infrared spectroscopy, powder X-ray diffraction, scanning electron microscopy, and energy dispersion X-ray. Kinetic adsorption studies for a group of 14 phenols (chlorophenols, alkylphenols, and bisphenols) present in waters and partitioning to the membrane systems were performed to gain insights into the extraction mechanisms of the membranes. The kinetics were fitted to pseudo-first order and interparticle diffusion models, with data indicating that chloro‑ and alkyl-phenols were adsorbed faster than bisphenols. Besides, the resulting membranes were utilized in thin film solid-phase microextraction (TF-SPME) in combination with high-performance liquid chromatography (HPLC) with diode array detection (DAD) and fluorescent detector (FLD). The optimized method using these mixed matrix membranes (with dish shape and 1 cm of diameter) required 60 min of extraction with agitation followed by 10 min of desorption in 500 µL of basic ethanol. Low limits of detection, down to 2.13 µg·L<sup>-1</sup>, were achieved, together with good performance in SPMS and BAGI metrics, with scores of 5.89 and 62.5, respectively. The performance with river waters was accompanied by adequate relative recoveries (74.3–117 %), reproducibility (with inter-day RSD values lower than 18 %), and absolute recoveries (up to 59 %).</div></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"13 ","pages":"Article 100150"},"PeriodicalIF":5.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143131054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extraction of synthetic cathinones from biological samples: A systematic review 从生物样本中提取合成卡西酮：系统综述

IF 5.2

Advances in Sample Preparation Pub Date : 2024-10-01 DOI: 10.1016/j.sampre.2024.100133

Mengjie Gu, Shutong Xue, Menghan Hou, Xiantao Shen

{"title":"Extraction of synthetic cathinones from biological samples: A systematic review","authors":"Mengjie Gu, Shutong Xue, Menghan Hou, Xiantao Shen","doi":"10.1016/j.sampre.2024.100133","DOIUrl":"10.1016/j.sampre.2024.100133","url":null,"abstract":"<div><div>Analysis of biological samples containing synthetic cathinones has become a significant area of interest for researchers in recent years, because synthetic cathinones can cause adverse reactions such as delusions and hallucinations. Generally, the intricate nature of the sample matrix and exceedingly low drug concentration in biological samples present significant challenges for the determination of target substances. Accordingly, scientists were dedicated to the advancement of effective sample preparation techniques to address these difficulties. However, the current reviews involved in synthetic cathinones all focused on detection methods. A systematic review focusing on sample preparation for the quantification of synthetic cathinones is still lacking. In light of that, this paper reviewed the most commonly used sample preparation techniques for synthetic cathinones. Recent advances in sample preparation techniques for synthetic cathinones in different biological samples such as blood, urine, oral fluid and hair samples were discussed. In addition, we prospected the challenges and the future perspectives of biological sample preparation techniques for synthetic cathinones determination.</div></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"12 ","pages":"Article 100133"},"PeriodicalIF":5.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142527745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0