Advances in Sample Preparation最新文献_第2页

Advances in magnetic nanomaterials for biomedical sample pretreatment: synthesis, functionalization, and applications in magnetic solid-phase extraction 生物医学样品前处理用磁性纳米材料的研究进展：合成、功能化及其在磁性固相萃取中的应用

IF 6.5

Advances in Sample Preparation Pub Date : 2025-08-01 DOI: 10.1016/j.sampre.2025.100208

Jiarui Zhang , Shuang Feng , Yalin Xi , Meiyun Shi , Lei Yin

{"title":"Advances in magnetic nanomaterials for biomedical sample pretreatment: synthesis, functionalization, and applications in magnetic solid-phase extraction","authors":"Jiarui Zhang , Shuang Feng , Yalin Xi , Meiyun Shi , Lei Yin","doi":"10.1016/j.sampre.2025.100208","DOIUrl":"10.1016/j.sampre.2025.100208","url":null,"abstract":"<div><div>Magnetic nanomaterials (MNMs) are highly effective sorbents for biomedical sample pretreatment, leveraging their superparamagnetism, large specific surface area, and tunable surface functionalities. This review comprehensively examines MNM classification, synthesis techniques, and functionalization approaches, highlighting their pivotal role in magnetic solid-phase extraction (MSPE) for analyte enrichment. We systematically detail MNM applications in biomedical sample processing, focusing on MSPE for efficient nucleotide isolation, specific protein capture, and ultrasensitive biomarker detection. While MNM-based MSPE offers significant advantages in sensitivity, selectivity, and operational simplicity, challenges remain in achieving reproducible synthesis and deepening the mechanistic understanding of adsorption processes. Future research should prioritize developing automated MSPE platforms and multifunctional MNMs capable of addressing complex clinical matrices. This work provides a valuable reference for designing next-generation analytical systems utilizing MNMs.</div></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"15 ","pages":"Article 100208"},"PeriodicalIF":6.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144903830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A green approach for electromembrane extraction of morphine from urine using sodium alginate-g-polyacrylic acid/agarose hydrogel membrane 海藻酸钠-聚丙烯酸/琼脂糖水凝胶膜电膜提取尿液中吗啡的绿色方法

IF 6.5

Advances in Sample Preparation Pub Date : 2025-08-01 DOI: 10.1016/j.sampre.2025.100204

Elnaz Khodabakhshi , Somayeh Makarem , Mehran Kurdtabar , Saeed Nojavan

{"title":"A green approach for electromembrane extraction of morphine from urine using sodium alginate-g-polyacrylic acid/agarose hydrogel membrane","authors":"Elnaz Khodabakhshi , Somayeh Makarem , Mehran Kurdtabar , Saeed Nojavan","doi":"10.1016/j.sampre.2025.100204","DOIUrl":"10.1016/j.sampre.2025.100204","url":null,"abstract":"<div><div>The development of novel membranes through green chemistry remains a significant challenge in advancing practical separation science. In this study, a sodium alginate-g-polyacrylic acid/agarose hydrogel was synthesized and, for the first time, applied as a membrane in gel electromembrane extraction (G-EME) for the isolation of morphine, a basic analyte, from urine samples. The extracted morphine was subsequently quantified using differential pulse voltammetry (DPV) with a glassy carbon electrode. The hydrogel membrane (5 mm thick) was prepared using 0.75 % (w/v) sodium alginate, 3.80 % (v/v) acrylic acid, 0.04 % (w/v) ammonium persulfate, and 1.25 % (w/v) agarose. The extraction process was optimized by evaluating factors such as membrane composition, extraction time, applied voltage, and pHs of the sample solution, membrane, and acceptor phase (AP). Under optimal conditions (25 min extraction time, 70 V applied voltage, membrane pH 4.0, AP pH 3.0, and sample solution pH 7.0) morphine was efficiently extracted through the hydrogel membrane and transferred to an aqueous AP. The collected AP was mixed with 0.15 M phosphate buffer (pH 7.0) and analyzed voltammetrically. The method achieved a limit of detection of 0.09 μg/mL and a limit of quantification of 0.28 μg/mL, and was successfully applied for morphine determination in urine sample. The polyacrylic acid-based membrane enhanced extraction efficiency through electrostatic interactions, while the use of DPV offered clearer signal interpretation and reduced background current, facilitating reliable detection of trace analytes. These advantages support the method’s potential for simple, selective, and environmentally friendly analysis of basic drugs in biological samples.</div></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"15 ","pages":"Article 100204"},"PeriodicalIF":6.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144780679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D-printed device for improved membrane-based extraction procedure of xenobiotics in complex matrices 3d打印装置，用于改进复杂基质中异种生物的膜基提取程序

IF 6.5

Advances in Sample Preparation Pub Date : 2025-08-01 DOI: 10.1016/j.sampre.2025.100205

Miryam Perrucci , Erika Maria Ricci , Abuzar Kabir , Kenneth G. Furton , Marcello Locatelli

{"title":"3D-printed device for improved membrane-based extraction procedure of xenobiotics in complex matrices","authors":"Miryam Perrucci , Erika Maria Ricci , Abuzar Kabir , Kenneth G. Furton , Marcello Locatelli","doi":"10.1016/j.sampre.2025.100205","DOIUrl":"10.1016/j.sampre.2025.100205","url":null,"abstract":"<div><div>To minimize the environmental and human health impacts of chemical processes, there is growing interest in eco-friendly methods aligned with the principles of Green Analytical Chemistry (GAC). A successful chemical analysis typically involves sample preparation, sampling, separation and analysis, quantification, and data interpretation. Among these, sample preparation plays a crucial role in isolating and preconcentrating target analytes from complex matrices.</div><div>In 2014, fabric phase sorptive extraction (FPSE) was introduced as a simplified and greener sample pretreatment method. Building on this advancement and a recent patent, the present study introduces an innovative 3D-printed device designed for use with a range of target analytes and complex sample matrices. Constructed from inert materials, the device features a rigid, modular structure with multiple windows that securely hold various membrane-based extraction materials. These include FPSE membranes, electrospun membranes, and materials derived from adsorbent systems recovered from production or usage waste, as well as permeable molecularly imprinted polymers (MIPs).</div><div>The device offers several advantages, including enhanced enrichment factors, compatibility with diverse planar membrane types, and highly customizable selectivity based on membrane configuration and chemistry. Its design also incorporates a built-in slot for a magnetic stirrer, enabling precise control of rotation speed during extraction-even <em>in field</em> conditions using a portable, battery-powered stirrer.</div><div>Experimental results unequivocally demonstrate that the new device achieves superior enrichment factors compared to previously validated methods for the same analytes, confirming its effectiveness and potential for broader analytical applications.</div></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"15 ","pages":"Article 100205"},"PeriodicalIF":6.5,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144780678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent green approaches in liquid-phase microextraction 液相微萃取的绿色新方法

IF 5.2

Advances in Sample Preparation Pub Date : 2025-07-17 DOI: 10.1016/j.sampre.2025.100201

Miguel Ángel Aguirre , Natalia Arroyo-Manzanares , Laura Carbonell-Rozas , José Grau , Carlos Moreno , Lorena Vidal

{"title":"Recent green approaches in liquid-phase microextraction","authors":"Miguel Ángel Aguirre , Natalia Arroyo-Manzanares , Laura Carbonell-Rozas , José Grau , Carlos Moreno , Lorena Vidal","doi":"10.1016/j.sampre.2025.100201","DOIUrl":"10.1016/j.sampre.2025.100201","url":null,"abstract":"<div><div>The miniaturization of conventional extraction techniques, particularly microextraction methods, has led to a reduction in the use of hazardous reagents and waste. However, the continued use of toxic solvents and materials, along with time-consuming sample preparation processes, negatively impacts the overall sustainability of these methods. As a result, there is growing interest in exploring greener alternatives. This review highlights the most promising green solvents for liquid-phase microextraction, including (natural) deep eutectic solvents, low-toxicity ionic liquids, surfactants, micellar solvents, and bio-based solvents, emphasizing their applications in innovative sample preparation techniques in the last five years. Particular emphasis is placed on their use in automated and semi-automated systems, in line with the increasing role of automation in Analytical Chemistry. Selected applications have been carefully reviewed and discussed to illustrate key advances in the use of green solvents and automation for extracting a wide range of compounds from various matrices. In addition, future perspectives are outlined to guide further development of liquid-phase microextraction in alignment with current trends in green sample preparation.</div></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"15 ","pages":"Article 100201"},"PeriodicalIF":5.2,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144685863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progress in functional covalent organic frameworks as advanced materials for sample pretreatment 功能共价有机骨架作为样品前处理先进材料的研究进展

IF 5.2

Advances in Sample Preparation Pub Date : 2025-07-11 DOI: 10.1016/j.sampre.2025.100200

Hui Cao , WeiKang Guo , Ke Liu , Qin Shuai , Lijin Huang , Zhaochu Hu

{"title":"Progress in functional covalent organic frameworks as advanced materials for sample pretreatment","authors":"Hui Cao , WeiKang Guo , Ke Liu , Qin Shuai , Lijin Huang , Zhaochu Hu","doi":"10.1016/j.sampre.2025.100200","DOIUrl":"10.1016/j.sampre.2025.100200","url":null,"abstract":"<div><div>Covalent organic frameworks (COFs) represent an emerging class of porous crystalline materials with immense potential as advanced materials for sample pretreatment. Their distinctive characteristics, including large specific surface areas, adjustable pore structures, robust chemical stability, and abundant active sites, render them a reliable platform for efficiently extracting analytes, thereby opening avenues for innovative applications in analytical chemistry. This review focuses on recent research progress in the utilization of functional COFs for the preconcentration of contaminants, including organic pollutants (such as drugs, pesticides, and dyes) and heavy metal ions. COFs featuring diverse functional groups, such as carboxyl, sulfonyl, hydroxyl, amino, nitro and halogen moieties and their integration with advanced sample pretreatment techniques are discussed. The review begins by examining two primary strategies for functionalizing COF: \"bottom-up\" and \"post-synthetic modification\". Subsequently, the interaction mechanisms and analytical performances of methods based on functionalized COFs are critically analyzed, emphasizing their extraction efficiency and selectivity. Finally, the technical merits of functional COFs as high-performance adsorbents in sample pretreatment are highlighted, while addressing current challenges and outlining future research directions. This review aims to provide a comprehensive reference for the rational design and practical applications of functionalized COFs in advanced sample pretreatment workflows.</div></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"15 ","pages":"Article 100200"},"PeriodicalIF":5.2,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144663689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A 3D-printed handheld device for quick citrus tissue lysis and nucleic acid extraction 用于快速柑橘组织裂解和核酸提取的3d打印手持设备

IF 5.2

Advances in Sample Preparation Pub Date : 2025-07-10 DOI: 10.1016/j.sampre.2025.100199

Chia-Wei Liu , Brent Kalish , Sohrab Bodaghi , Georgios Vidalakis , Hideaki Tsutsui

{"title":"A 3D-printed handheld device for quick citrus tissue lysis and nucleic acid extraction","authors":"Chia-Wei Liu , Brent Kalish , Sohrab Bodaghi , Georgios Vidalakis , Hideaki Tsutsui","doi":"10.1016/j.sampre.2025.100199","DOIUrl":"10.1016/j.sampre.2025.100199","url":null,"abstract":"<div><div>A 3D-printed handheld device has been developed for rapid and efficient sample preparation, extracting pathogen total nucleic acids from citrus leaves for downstream molecular analysis. With its high-speed motor, knurled lysis chamber for rapid sample lysis, and quick nucleic acid extraction using cellulose paper disks (Whatman CHR1), this device can yield ready-to-use DNA and RNA in just 12 min. All components, except for the motor, wiring, and paper disks, were printed in-house using a PolyJet 3D printer with photosensitive resins. The device was optimized for maximum sample lysis by evaluating operation voltages and chamber features, as measured by DNA and RNA concentrations using a Qubit fluorometer and quantification cycle (Cq) values obtained through qPCR assays. The results showed that the lysis chamber with internal knurling and the motor operated at 7.5 V was sufficient for effective sample lysis in 1 min, achieving RNA concentrations up to 87.6 % of those obtained with mortar-and-pestle grinding. Paper disk washing and elution conditions were also optimized using a NanoDrop spectrophotometer and qPCR assays, where two 1-minute washes and a 100 µL elution volume resulted in the highest purity and lowest Cq value. The optimized handheld device was validated with citrus sources infected with <em>Citrus tristeza virus</em> (CTV) and <em>Spiroplasma citri</em> (<em>S. citri</em>), demonstrating high detection accuracy (100 %) and low assay variation (CV < 3.8 %) in qPCR-based analysis. Based on these successful results, this device is expected to be broadly applicable to similar viral and bacterial pathogens affecting plants.</div></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"15 ","pages":"Article 100199"},"PeriodicalIF":5.2,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144634324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pretreat immunosuppressants in whole blood without vortexing and centrifugation 在全血中预处理免疫抑制剂，不进行旋流和离心

IF 5.2

Advances in Sample Preparation Pub Date : 2025-07-07 DOI: 10.1016/j.sampre.2025.100198

Rui Zhang , Ren-Jie Yang , Ping-An Zhang , Shao-Ting Wang

{"title":"Pretreat immunosuppressants in whole blood without vortexing and centrifugation","authors":"Rui Zhang , Ren-Jie Yang , Ping-An Zhang , Shao-Ting Wang","doi":"10.1016/j.sampre.2025.100198","DOIUrl":"10.1016/j.sampre.2025.100198","url":null,"abstract":"<div><h3>Background</h3><div>Precise measurement of immunosuppressant levels in whole blood is critical for monitoring post-transplant patient outcomes. Conventional protein precipitation (PP) methods, which rely on vortex mixing and centrifugation, present substantial limitations in terms of automation and scalability. To address these challenges, we developed a novel pretreatment strategy termed “Pseudo-Protein-Precipitation combined with Cold-Induced Phase Separation” (PPP+CIPS), designed to simplify sample processing and enhance high-throughput efficiency.</div></div><div><h3>Results</h3><div>The PPP+CIPS method employs 48 % acetonitrile to generate a semi-homogeneous blood suspension, enabling in-situ drug extraction via CIPS. Notably, this approach eliminates the need for vortexing and centrifugation—key bottlenecks in traditional therapeutic drug monitoring workflows. By leveraging 96-well plates and multi-channel pipettes, the protocol reduces pretreatment time to approximately one-third of that required by PP. Clinical validation (<em>n</em> = 288 in total) revealed strong concordance with established methods, with 94 % of tacrolimus, 95 % of cyclosporin A, and 92 % of sirolimus measurements falling within ±20 % agreement limits.</div></div><div><h3>Significance</h3><div>The PPP+CIPS strategy marks a significant leap forward in high-throughput therapeutic drug monitoring for immunosuppressants. Its seamless integration with 96-well formats and static processing workflows makes it a promising cornerstone for future automated and integrated TDM systems.</div></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"15 ","pages":"Article 100198"},"PeriodicalIF":5.2,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144595951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supported liquid membrane-coupled Extraction 支撑液膜耦合萃取

IF 5.2

Advances in Sample Preparation Pub Date : 2025-07-07 DOI: 10.1016/j.sampre.2025.100197

Shengjie Hu , Xiantao Shen , Chuixiu Huang

{"title":"Supported liquid membrane-coupled Extraction","authors":"Shengjie Hu , Xiantao Shen , Chuixiu Huang","doi":"10.1016/j.sampre.2025.100197","DOIUrl":"10.1016/j.sampre.2025.100197","url":null,"abstract":"<div><div>Group separation of multiple analytes with diverse physicochemical properties from the same complex sample is significant in clinical diagnosis, environmental monitoring and biomarker identification. To achieve this goal, supported liquid membrane (SLM)-coupled extraction techniques were developed by integrating two or more SLM-based extraction processes, either simultaneously or sequentially. This approach offers several advantages such as low sample consumption, short operational time, and efficient group separation. In recent years, SLM-coupled extraction approaches have been successfully applied to extract multiple targets from complex biological samples and environmental samples. However, the reviews in the literature mainly focused on a single SLM extraction technique, either liquid-phase microextraction (LPME) or electromembrane extraction (EME). Therefore, a comprehensive overview of SLM-coupled extraction techniques is still lack. The current review comprehensively summarizes the advances in SLM-coupled extraction techniques. We elaborate three types of SLM-coupled extraction techniques, including coupled-LPME, coupled-EME and EME/LPME. Moreover, a prospective discussion on the future development trends of the SLM-coupled extraction techniques is also provided.</div></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"15 ","pages":"Article 100197"},"PeriodicalIF":5.2,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144632451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced electromembrane extraction of acidic drugs using a chitosan-agarose hybrid gel membrane 壳聚糖-琼脂糖混合凝胶膜对酸性药物电膜萃取的强化作用

IF 5.2

Advances in Sample Preparation Pub Date : 2025-06-30 DOI: 10.1016/j.sampre.2025.100196

Hasti Gharah Sheikhlou , Majid Gharah Sheikhlou , Marzieh Saberi , Saeed Nojavan

{"title":"Enhanced electromembrane extraction of acidic drugs using a chitosan-agarose hybrid gel membrane","authors":"Hasti Gharah Sheikhlou , Majid Gharah Sheikhlou , Marzieh Saberi , Saeed Nojavan","doi":"10.1016/j.sampre.2025.100196","DOIUrl":"10.1016/j.sampre.2025.100196","url":null,"abstract":"<div><div>In this study, a novel chitosan-agarose gel membrane was developed and applied in the gel electromembrane extraction (G-EME) technique to extract and preconcentrate two model acidic drugs, naproxen and ibuprofen, from urine samples. Chitosan, acting as a positively charged agent and viscosifier, significantly improved extraction efficiency by reducing electric current and minimizing the electroendosmosis (EEO) effect. The parameters affecting the extraction recoveries were optimized and evaluated using both one-variable-at-a-time and Box–Behnken design approaches alongside response surface methodology. To maximize drugs recovery from a 7.0 mL urine sample, the following parameters were optimized: a membrane composed of 3.0 % (w/v) agarose and 1.0 % (w/v) chitosan, pH of the gel membrane: 5.5, extraction voltage: 32.5 V, pH of the donor phase: 8.0, pH of the acceptor phase: 11.0, extraction time: 21 min, volume of acceptor phase: 200 µL, gel membrane thickness: ∼ 4 mm and donor phase stirring speed: 500 rpm. Under optimal conditions, the extraction recoveries were 60.4 % for naproxen and 81.9 % for ibuprofen. Limits of detection for naproxen and ibuprofen were 3.0 and 4.5 ng/mL, respectively. This method was successfully applied to quantify acidic drugs in real urine samples, achieving relative recoveries in the range of 91.2–94.9 %, demonstrating excellent reliability for biological matrices.</div></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"15 ","pages":"Article 100196"},"PeriodicalIF":5.2,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144570583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of performance and matrix compatibility of mixed mode C18-SCX SPME fibers for compounds with different physicochemical properties 混合模式C18-SCX SPME纤维对不同理化性质化合物的性能及基质相容性评价

IF 5.2

Advances in Sample Preparation Pub Date : 2025-06-26 DOI: 10.1016/j.sampre.2025.100195

Marcos Tascon , Ezel Boyaci , Nathaly Reyes-Garcés , Janusz Pawliszyn

{"title":"Evaluation of performance and matrix compatibility of mixed mode C18-SCX SPME fibers for compounds with different physicochemical properties","authors":"Marcos Tascon , Ezel Boyaci , Nathaly Reyes-Garcés , Janusz Pawliszyn","doi":"10.1016/j.sampre.2025.100195","DOIUrl":"10.1016/j.sampre.2025.100195","url":null,"abstract":"<div><div>Solid-phase microextraction (SPME) has evolved significantly since its inception, yet challenges remain in developing coatings fully compatible with LC-MS that combine broad polarity coverage with biocompatibility for complex matrices. This study evaluates mixed-mode C<sub>18</sub>-SCX (strong cation exchange) SPME fibers designed to extract analytes of a wide range of physicochemical properties, addressing limitations in current methodologies. The fibers were tested for extraction efficiency, reproducibility, and matrix compatibility using a group of model compounds with different physicochemical properties, namely, codeine (logP=1.19), carbamazepine (logP=2.45), diazepam (logP=2.82), and propranolol (logP=3.47). Furthermore, the biocompatibility was tested in diverse matrices, such as PBS, blood, plasma, urine, and grape juice.</div><div>Results demonstrated exceptional inter-fiber reproducibility (RSD ≤ 15 %, <em>n</em> = 96 fibers) and robust performance in biomatrices, with relative matrix effects primarily governed by analyte binding affinities to matrix macromolecules rather than coating fouling. Absolute matrix effects were negligible (93–111 %), underscoring the fibers’ ability to deliver clean extracts for LC-MS analysis. Fiber reusability was validated over three consecutive extractions (RSD ≤ 10 %), and morphological integrity was preserved post-extraction, even in challenging matrices like whole blood. This work represents the versatility of mixed-mode SPME fibers for high-throughput bioanalysis, offering a significant advancement for in vivo and in vitro targeted and untargeted applications.</div></div>","PeriodicalId":100052,"journal":{"name":"Advances in Sample Preparation","volume":"15 ","pages":"Article 100195"},"PeriodicalIF":5.2,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144549565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0