涂布叶片喷雾质谱法测定完整脑肿瘤中的酰基肉碱

IF 6.5 Q1 CHEMISTRY, ANALYTICAL
Joanna Bogusiewicz , Karol Jaroch , Jacek Furtak , Marcin Birski , Krystyna Soszyńska , Anna Majdańska , Agata Ryfa , Marek Harat , Barbara Bojko
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引用次数: 0

摘要

癌症改变的潜在生物标志物可以通过分析广泛的分析物来选择。为此,应用了能够对不同生物基质进行详细分析的分析平台。然而,在生物标志物选择之后,必须尽可能缩短分析时间以加快诊断过程。因此,本研究旨在优化和测试涂层叶片喷雾质谱(CBS-MS)分析脑肿瘤研究中潜在生物标志物酰基肉碱的方法。由于NF2突变与恶性转化和多发性脑膜瘤的高风险相关,我们对不同突变状态的脑膜瘤进行了研究。应用CBS-MS可以测定完整肿瘤组织中乙酰肉碱(AC C2:0)和丙基肉碱(AC C3:0)的浓度。然而,有必要扩大研究小组,以证实NF2突变型和野生型脑膜瘤中这些分析物浓度的结论。除了定性分析外,还可以在非靶向分析中同时检测到大范围的酰基肉碱。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Determination of acylcarnitines in intact brain tumors using coated blade spray mass spectrometry (CBS-MS)

Determination of acylcarnitines in intact brain tumors using coated blade spray mass spectrometry (CBS-MS)
Potential biomarkers of cancerous alterations could be selected by profiling a wide range of analytes. For this purpose, analytical platforms enabling detailed analysis of different biological matrices are applied. However, after biomarker selection, it is essential to shorten the analysis time as much as possible to speed up the diagnostic process. Therefore, this study aimed to optimize and test the coated blade spray mass spectrometry (CBS-MS) method in analyzing potential biomarkers in brain tumor studies: acylcarnitine. Meningiomas with different mutation statuses in NF2 were studied due to the relation of NF2 mutation with the higher risk of malignant transformation and multiple meningiomas. The application of CBS-MS enabled the determination of acetylcarnitine concentration (AC C2:0) and propionylcarnitine (AC C3:0) in intact tumor tissue. However, it would be necessary to enlarge the study group to confirm the conclusions on the concentration of these analytes in NF2 mutant and wild-type meningiomas. Apart from qualitative analysis, the simultaneous detection of a wide range of acylcarnitines in untargeted analysis was also possible.
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