Springer最新文献

{"title":"A novel image encryption scheme with synchronous permutation–diffusion controlled by CA and a sine-cosine-optimized CML model","authors":"Jiakun Wang,&nbsp;Zhen Li,&nbsp;Weijie Tan,&nbsp;Xianming Wu","doi":"10.1140/epjp/s13360-025-06767-2","DOIUrl":"10.1140/epjp/s13360-025-06767-2","url":null,"abstract":"<div><p>A novel sine-cosine-optimized coupled map lattices (SCOCML) is proposed, which integrates the cellular automata (CA)-guided non-adjacent coupling method and is constructed upon the sine-cosine-optimized (SCO) map. Within the given parameter range, dynamics analysis shows that every lattice point in the proposed SCOCML system sustains a chaotic state, thereby exhibiting enhanced chaotic features. Based on the SCOCML system, a novel image encryption scheme is presented. It starts with generating a plaintext-related initial key, ensuring high sensitivity to plain images. Additionally, a pseudo-random number generator (PRNG) based on the dynamic gas cellular automata (DGCA) model is proposed to further improve the randomness of chaotic sequences, enabling the image cryptosystem to achieve robust security performance. Subsequently, a CA-controlled synchronous permutation–diffusion method is proposed, which utilizes the evolving states of cellular automata to dynamically perform row and column encryption, achieving an efficient and coordinated encryption effect in merely three rounds. Extensive experimental results and security analyses confirm that the proposed image cryptosystem offers excellent resistance against various attacks, achieving information entropy &gt; 7.999, NPCR &gt; 99.60, and UACI <span>(approx)</span> 33.45, making it suitable for practical secure image communication applications.\u0000</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":792,"journal":{"name":"The European Physical Journal Plus","volume":"140 9","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144998394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aerobic and anaerobic biodegradation of 1,2,3-trichloropropane and 1,2-dichloropropane: implications for bioremediation","authors":"Paul B. Hatzinger,&nbsp;Rachael Rezes,&nbsp;Simon Vainberg,&nbsp;Paul Koster van Groos","doi":"10.1007/s10532-025-10178-6","DOIUrl":"10.1007/s10532-025-10178-6","url":null,"abstract":"<div><p>1,2,3-Trichloropropane (1,2,3-TCP) is a suspected human carcinogen and a persistent emerging contaminant in groundwater and drinking water. 1,2,3-TCP was historically used as a solvent for cleaning and maintenance, paint and varnish removal, and degreasing, but its sources also include chemical manufacturing processes and application of soil fumigants. The California Department of Public Health (CDPH) has established a state maximum contaminant level (MCL) of 0.005 µg/L for 1,2,3-TCP in drinking water and a public health goal (PHG) of only 0.0007 µg/L. The primary research question addressed herein was whether aerobic or anaerobic cultures can potentially be applied for treatment of 1,2,3-TCP, and whether bacteria are capable of biodegrading 1,2,3-TCP to below the California MCL. During this study, we identified cultures capable of biodegrading 1,2,3-TCP via reductive dehalogenation as well as through aerobic cometabolic processes. Follow-on studies with organisms capable of aerobically degrading 1,2,3-TCP included kinetic modeling and assessment of concentrations of 1,2,3-TCP achievable via biodegradation. 1,2-Dichloropropane (1,2-DCP) is sometimes found co-mingled with 1,2,3-TCP, so studies also were conducted to quantify rates of 1,2-DCP biodegradation alone and when present together with 1,2,3-TCP. The dehalogenating consortium CPD-2, which was isolated from sewage sludge and includes <i>Dehalococcoides, Dehalobacter</i> and <i>Dehalobium</i> spp., biodegraded both 1,2,3-TCP and 1,2-DCP. Anaerobic 1,2,3-TCP degradation resulted in a transient production of 1,2-DCP followed by 1-chloropropane (1-CP), which accumulated nearly stoichiometrically and then slowly degraded, indicating complete dechlorination of 1,2,3-TCP by this mixed culture. Two different cometabolic pure cultures, <i>Rhodococcus ruber</i> ENV425 and <i>Rhodococcus aetherivorans</i> ENV493 degraded 1,2,3-TCP after growth on propane or isobutane. Importantly, both bacteria were capable of degrading 20 µg/L of 1,2,3-TCP to &lt; 0.005 µg/L after growth on isobutane. Experiments conducted with ENV425 and ENV493 to quantify relevant kinetic parameters after growth on isobutane suggested that ENV425 facilitated more rapid 1,2,3-TCP degradation than ENV493. Both strains were observed to degrade 1,2-DCP much faster than 1,2,3-TCP when present individually or in mixtures. The data from this study suggest that cometabolic treatment of 1,2,3-TCP, or mixtures of 1,2-DCP and 1,2,3-TCP, is feasible and that relevant regulatory concentrations are achievable using this process. Similarly, anaerobic treatment may be possible at locations with higher concentrations or where 1,2,3-TCP occurs with other chlorinated solvents.</p></div>","PeriodicalId":486,"journal":{"name":"Biodegradation","volume":"36 5","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144998414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-energy factorization via eigenfunctions of the next-to-leading-order BFKL kernel","authors":"Ada Polizzi,&nbsp;Michael Fucilla,&nbsp;Alessandro Papa","doi":"10.1140/epjc/s10052-025-14668-y","DOIUrl":"10.1140/epjc/s10052-025-14668-y","url":null,"abstract":"<div><p>We present a general formula for the amplitude of forward exclusive hadronic processes in the semihard regime of perturbative Quantum Chromodynamics (QCD), by means of the <i>next-to-leading order</i> eigenfunctions of the Balitsky–Fadin–Kuraev–Lipatov (BFKL) kernel, as constructed by Chirilli and Kovchegov. We discuss some formal subtleties in the check of compatibility with the similar formula based on the use of the <i>leading-order</i> BFKL eigenfunctions. Finally, in the specific case of the electroproduction of two light vector mesons, we consider the numerical stability of the amplitude when one or the other set of eigenfunctions is adopted.\u0000</p></div>","PeriodicalId":788,"journal":{"name":"The European Physical Journal C","volume":"85 9","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1140/epjc/s10052-025-14668-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144998480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Berberine contributes to protecting against the cadmium-induced pancreatic damage: role of intestinal microbiome modulation and barrier function","authors":"Abdulaziz Q. Ali,&nbsp;Ezat A. Mersal,&nbsp;Rastam Samer,&nbsp;Samaa A. Alhjmohammad,&nbsp;Zainab H. Alabdrabalridha,&nbsp;Fatimah Y. Alseeni,&nbsp;Amal F. Dawood,&nbsp;Marwa Omar Abdel All,&nbsp;Ahmed M. Abdelmoneim,&nbsp;Tamer M. Shawky","doi":"10.1007/s10735-025-10591-4","DOIUrl":"10.1007/s10735-025-10591-4","url":null,"abstract":"<div><p>Cadmium (Cad) is a worldwide heavy metal pollutant associated with global health challenges. Alteration of the intestinal microbiome, due to chemicals’ exposure, plays a vital role in the pathogenesis of gastrointestinal diseases such as pancreatic disorders. Hence, modulation of the gut microbiota might be a targeted approach to manage pancreatic diseases. Using murine modeling, this study consisted of two dependent experiments to investigate the curative potential of berberine (BBR) in a Wistar rat model of Cad-provoked pancreatic toxicity and the possible contribution of gut microbiota to BBR protection. In experiment 1, Cad-induced pancreatic injury was established in rats via 8-week oral gavage of Cad at 4 mg/kg. The treatment group was exposed to BBR at 200 mg/kg body weight, oral gavage for 8 weeks. In experiment 2, transplantation of the fecal microbiome was done, in which the fecal microbiota in each group of experiment 1 was orally gavaged to the healthy rats of each corresponding group in experiment 2, once weekly for 8 weeks. The serum amylase and lipase levels, pancreatic inflammatory and oxidative markers, histological, and immunohistochemical analyses were evaluated. The markers of gut mucosal barrier, and mRNA expression of cell junction proteins were investigated for possible intestinal injury. 16S rRNA sequencing was applied to identify the gut bacterial changes and possible pancreatic bacterial translocation. Cad induced intestinal barrier disruption and elicited a state of pancreatic inflammation and apoptosis as indicated by TGF-β and BAX immunohistochemistry, which were relieved by BBR. A decreased firmicutes/bacteroidetes ratio and microbial migration due to interrupted intestinal mucosal barrier were reported. Furthermore, BBR restored the bacterial richness and proportions in the gut, thereby maintaining the intestinal microbial community, fixing the intestinal mucosal barrier structure, and inhibiting the pathway of bacterial migration. BBR protected against Cad-induced pancreatic damage, mostly through safeguarding the intestinal barrier function. Modulation of the intestinal bacterial community, repairing the gut barrier structure, and interference with the pancreatic bacterial migration and colonization were suggested BBR effects, potentially alleviating Cad-related pancreatic injury.</p></div>","PeriodicalId":650,"journal":{"name":"Journal of Molecular Histology","volume":"56 5","pages":""},"PeriodicalIF":2.2,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144998561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dissipative particle dynamics simulations of wormlike chain adsorption onto planar walls in a pressure-driven flow","authors":"Shaofeng Xu,&nbsp;Zichen Wang,&nbsp;Yifan Yu,&nbsp;Chuzhen Zhu,&nbsp;Wei Zhang","doi":"10.1007/s10965-025-04468-z","DOIUrl":"10.1007/s10965-025-04468-z","url":null,"abstract":"<div><p>Adsorption of wormlike chain onto planar walls in a pressure-driven flow was investigated using dissipative particle dynamics (DPD) method. The wormlike chain was modeled by the well-known bead-spring chain connected by the Marko-Siggia spring. Under static condition, the wormlike chain is adsorbed onto the wall in an extended conformation and forms trains for a strongly adsorbed wall, while sections of the chain are adsorbed and the chains form loops and tails for a weakly adsorbed wall. In the presence of pressure-driven flow, our simulations show that the chain is stretched near the wall, and the chain-wall hydrodynamic interaction would lead to the desorption of the wormlike chain. The desorption is determined by an interplay between wall-chain attractive interaction and chain-wall hydrodynamic interaction. Increasing the flow strength would increase the chain-wall hydrodynamic interaction, thereby leading to a stronger desorption. The desorption rate <i>η</i> of worm-like chain is found to scale as <span>(nproptodotgamma^{0.6865})</span> with shear rate <span>(dotgamma)</span>. For a strongly adsorbed wall, all sections of wormlike chain are adsorbed with trains conformation, which causes the chain-wall hydrodynamic interaction to be screened.</p></div>","PeriodicalId":658,"journal":{"name":"Journal of Polymer Research","volume":"32 9","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144998567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On estimating the threshold voltage of vertical junctionless GaN power fin-MOSFETs","authors":"Smriti Singh,&nbsp;Ankita Mukherjee,&nbsp;Aasim Ashai,&nbsp;Tanmoy Pramanik,&nbsp;Biplab Sarkar","doi":"10.1007/s10825-025-02406-2","DOIUrl":"10.1007/s10825-025-02406-2","url":null,"abstract":"<div><p>Traditional analytical models derived for Si logic devices fail to estimate the threshold voltage (<i>V</i>_<i>TH</i>) of vertical junctionless GaN power Fin-channel metal oxide semiconductor field effect transistors (VJ GaN Fin-MOSFETs). Solving two-dimensional Poisson’s equation inside the drift region of VJ GaN Fin- MOSFETs is not a viable option as of now. Thus, we report an alternate methodology to derive the analytical model for estimating the <i>V</i>_<i>TH</i> of VJ GaN Fin-MOSFETs. The proposed model uses an available baseline model followed by adding tuning parameters to the baseline model via a standard procedure. The proposed model faithfully predicts the effect of crucial geometrical and bias parameters on <i>V</i>_<i>TH</i>; along with estimating the drain induced barrier lowering effect. The proposed methodology is generic in nature; it can be applied to other (ultra)wide bandgap semiconductor based VJ power MOSFETs that are currently under extensive investigation.</p></div>","PeriodicalId":620,"journal":{"name":"Journal of Computational Electronics","volume":"24 6","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145005500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flood risk assessment under land use dynamics during 2000–2020 in the yellow river basin, China","authors":"Tongqing Liu,&nbsp;Shuxia Sun,&nbsp;Naixian Wang,&nbsp;Renqing Wang,&nbsp;Peiming Zheng,&nbsp;Hui Wang","doi":"10.1007/s12665-025-12526-4","DOIUrl":"10.1007/s12665-025-12526-4","url":null,"abstract":"<div><p>Floods are one of the most devastating natural disaster events globally and are strongly influenced by land use changes. This study crafted a holistic “hazard-sensitivity-vulnerability-restorability” flood risk assessment framework, integrating data from land use, natural environment, and socioeconomic factors. Using the Yellow River Basin (YRB) in China as an example from 2000 to 2020, it analyzed the spatiotemporal evolution of land use structure and flood risk, revealing their cluster pattern based on quantitative land use structure indices and flood risk index. Key findings include: (1) A gradient pattern of increasing flood risk from northwest to southeast in the YRB, with an overall trend of significant decrease followed by rebound from 2000 to 2020. (2) By 2020, land use homogenization occurred in middle-basin deserts due to grassland restoration, contrasting with down-basin fragmentation driven by built-up land expansion. (3) Global spatial correlation showed flood risk positively linked to cropland, built-up land and forest significantly but negatively associated with grassland significantly, highlighting land use trade-offs in flood mitigation. (4) Local spatial correlation revealed a “cold west (low risk - low structure index) vs. hot east (high risk – high structure index) \" differentiation, emphasizing zoning-based management needs. The study provides actionable insights for balancing flood resilience and land resource sustainability in the YRB. The proposed framework offers a transferable methodology for large river basins globally, particularly in regions facing coupled pressures of climate change and rapid urbanization.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":542,"journal":{"name":"Environmental Earth Sciences","volume":"84 18","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145005555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Label-free immunoassay based on chemiluminescence-functionalized magnetic mesoporous nanoparticles for rapid detection of neuron-specific enolase","authors":"Wenxia Chen,&nbsp;Shuangyi Hu,&nbsp;Bingqian Zhou,&nbsp;Yueran Ren,&nbsp;Yangyang Xue,&nbsp;Rui Yang","doi":"10.1007/s00604-025-07475-5","DOIUrl":"10.1007/s00604-025-07475-5","url":null,"abstract":"<div><p>Lung cancer, as one of the cancers with the highest morbidity and mortality rates in the world, requires accurate detection of its vital serum marker, neuron-specific enolase (NSE), which is a key challenge for early detection of lung cancer. However, traditional chemiluminescence immunoassay (CLIA) methods rely on labeled antibodies (Abs) and suffer from complex operations and high costs. In this work, a label-free CLIA based on CL-functionalized mesoporous magnetic nanoparticles (CuFe₂O₄@mSiO₂-Cys-Luminol-Au NPs) is developed for the rapid and sensitive detection of NSE. The material combines the advantages of magnetic separation, mesoporous loading, catalytic enhancement, and biorecognition. CuFe₂O₄ NPs offer efficient magnetic separation, while the mesoporous silica (mSiO₂) layer is effectively loaded with luminol to amplify CL signals. L-cysteine (L-Cys) ensures the uniform distribution of luminol and gold nanoparticles (Luminol-Au NPs), with these Au NPs immobilizing Abs through Au–S and Au–N bonds, achieving high-density biocoupling. The constructed label-free CLIA enables highly sensitive detection of NSE without Abs labeling. The results show that the NSE concentration is linearly correlated with the CL intensity in the range 1.0 × 10⁻¹³ ~ 1.0 × 10⁻⁸ g/mL, and the detection limit is as low as 2.3 × 10⁻¹⁴ g/mL. The proposed method demonstrates excellent selectivity, stability, and anti-interference capability for the detection of NSE in human serum samples.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145005558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly sensitive detection and mechanism study of tetracycline in water by CuFeO2/NiCo-LDH heterojunction electrochemical sensor","authors":"Aijin Xiao,&nbsp;Meng Li,&nbsp;Yiluo Hu,&nbsp;Wei Zhang,&nbsp;Huiping Bai,&nbsp;Xuejun Pan,&nbsp;Fengzhi Jiang","doi":"10.1007/s00604-025-07508-z","DOIUrl":"10.1007/s00604-025-07508-z","url":null,"abstract":"<div><p>A CuFeO₂/NiCo-LDH heterojunction electrochemical sensor (LDH: layered double hydroxide) was developed for the sensitive detection of tetracycline (TC). The sensor was constructed by integrating ZIF-67-derived nanocage NiCo-LDH on nickel foam with CuFeO₂, forming a p-n heterojunction that enhanced electron transfer and TC adsorption. The sensor exhibited bilinear detection ranges (0.00594 ~ 1 and 1 ~ 25 μM) and a low detection limit of 1.98 nM. Recoveries in real water samples ranged from 92.0 to 109.0%, with relative standard deviations not exceeding 4.5%. In situ Raman spectroscopy and density functional theory calculations confirmed the involvement of multiple redox pairs (Ni³⁺/Ni²⁺, Co³⁺/Co²⁺, Fe³⁺/Fe²⁺ and Cu²⁺/Cu⁺) and strong interfacial adsorption energy. This sensor presents a practical and efficient approach for onsite TC monitoring.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145005559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterologous Expression, Purification, and Characterization of a High-Temperature Requirement A (HtrA) Protease Homologue from Leptospira.","authors":"Preeti Kumari, Garima Singh, Samarendra K Singh, Padikara K Satheeshkumar","doi":"10.1007/s12010-025-05368-0","DOIUrl":"https://doi.org/10.1007/s12010-025-05368-0","url":null,"abstract":"<p><p>Leptospirosis is a zoonotic disease affecting humans in the tropical and temperate regions. Considerably high mortality rate (60 per 1000 adult) and associated morbidity necessitate the need for efficient diagnostic and therapeutic approaches for this disease. Proteins that play crucial roles in the invasion/pathogenesis are potential candidates for the diagnosis/therapeutics. High temperature requirement A (HtrA) is a protein expressed by many pathogenic bacteria, important for their virulence and survival. In this study, we have amplified, cloned, and expressed one of the HtrA homologues (HtrA1) from Leptospira. The expressed recombinant HtrA was purified using Ni-NTA chromatography. Physicochemical characterization of the enzyme using azo-casein substrate showed the maximum activity at a temperature 42 °C and pH 7. While Mn²⁺ showed significant positive effect, all the other tested metals inhibited the enzymatic activity, sometimes up to 97% as in the case of Cu²⁺. All the protease inhibitors inhibited the enzymatic activity with PMSF having maximum efficiency. The host cells expressing HtrA showed growth inhibition in a time-bound manner. Docking analysis identified the crucial amino acids involved in the interaction with cell junction proteins like E-cadherin, occludin, claudin-8, and desmoglein-2. Treating adherent mammalian cells with the recombinant protein showed the disruption of cell adherence, and the western analysis of the protein samples collected from the same experiment indicated the cell junction protein cleavage when probed with anti-E-cadherin antibody.</p>","PeriodicalId":465,"journal":{"name":"Applied Biochemistry and Biotechnology","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145005716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}