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{"title":"Knockdown of CPSF4 Inhibits Bladder Cancer Cell Growth by Upregulating NRF1.","authors":"Yixiang Sun, Guanglei Li, Hanlin Zhang, Mao Xie","doi":"10.1007/s10528-024-10891-6","DOIUrl":"10.1007/s10528-024-10891-6","url":null,"abstract":"<p><p>Increasing studies have shown that nuclear respiratory factor 1 (NRF1) deficiency frequently occurs in many human diseases, and its activation can protect neurons and other cells from degenerative diseases and malignant tumors. However, how NRF1 is regulated in bladder cancer remains unknown. Our research aims to reveal the role of leavage and polyadenylation-specific factor 4 (CPSF4) on the growth inhibition effect of bladder cancer and clarify its relationship with NRF1. Here, cell proliferation assay, transwell migration assay and multicellular tumor spheroids (MCTS) formation assay in the bladder cancer cell lines were carried out to measure tumor cell growth. Western bolt assay was carried out to identify the relationship between NRF1 and CPSF4. Also, subcutaneous xenograft tumors in nude mice were established to further validate the inhibition effect of CPSF4 on bladder tumor and the regulation on NRF1. The results in vitro showed that knockdown of CPSF4 strongly reduced the proliferation and migration, and inhibited MCTS formation in 5637 and HT1376 cell lines, while an additional knockdown of increased NRF1 induced by CPSF4 knockdown partially abolished these effects. The results in vivo showed that knockdown of CPSF4 strongly reduced the volume and weight of subcutaneous tumor, and decreased the expression of Ki-67 in tumor tissue, while NRF1 knockdown partially reversed these effects induced by CPSF4 knockdown. Western bolt assay demonstrated that CPSF4 could negatively regulate NRF1. Our results indicated that knock-down of CPSF4 inhibited bladder cancer cell growth by upregulating NRF1, which might provide evidence of CPSF4 as a therapeutic target for bladder cancer.</p>","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":"3517-3532"},"PeriodicalIF":2.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141747072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing Pandemic Impacts to Collaborative Management in Parks and Protected Areas.","authors":"Allie McCreary, Erin Seekamp, Michael B Edwards","doi":"10.1007/s00267-025-02207-0","DOIUrl":"10.1007/s00267-025-02207-0","url":null,"abstract":"<p><p>Partnerships are key in helping public land management agencies complete mission-critical conservation work and maintain agency relevancy through community engagement. While there had been a growing trend toward collaboration for many public agencies, the COVID-19 pandemic challenged the ability of volunteers and other groups to work with land management personnel. This study examined public agency personnel's reported work accomplishments with partners and perceptions of partnerships before (2019), during (2020), and the year after (2021) the pandemic. Results indicate that partnership engagement on conservation-related tasks declined during the pandemic. While the volume of work somewhat recovered in 2021, there remained impacts to the types of partners personnel worked with and to personnel's perceptions of institutional emphasis on partnerships. Implications for public agencies and their partners include increased emphasis on finding partnership 'champions' within the agency and umbrella organizations outside of the agency to facilitate partnership arrangements.</p>","PeriodicalId":543,"journal":{"name":"Environmental Management","volume":" ","pages":"2136-2149"},"PeriodicalIF":2.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12259788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144332256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Linking Forest Planning and Recreational Trail Design: A GIS Approach for Enhancing the Social Use of Forests.","authors":"Aitor Àvila Callau, Maitane Erdozain, Estela Inés Farías-Torbidoni, Sergio de-Miguel","doi":"10.1007/s00267-025-02199-x","DOIUrl":"10.1007/s00267-025-02199-x","url":null,"abstract":"<p><p>Forests planned for social use are classified as such due to the cultural ecosystem services they offer. To fully benefit from these services, forest stands for social use must be easily accessible and interconnected, not just through forest roads but also through recreational trails, a key outdoor leisure infrastructure. However, forest planning and trail design are seldom integrated. This study addresses this issue by proposing a method to create connector routes between official trails and forest stands managed for social objectives (FSMSO), enhancing the socio-recreational use of forests. Using Geographic Information Systems (GIS), our approach analyses overlap between official trails and FSMSO, identifies direct routes with origin-destination matrices and assesses FSMSO accessibility. Route viability was then calculated, supporting decision-makers in assessing route homologation potential. In our study area (Catalonia, Spain), findings show that only 14% of the FSMSO overlap with official trails. Among those not overlapping, 75% are connected with official trails via the road network, while 25% are inaccessible. Of the accessible stands from official trails, 54% are more than 20 min away on foot, while 22% are within 20 min. Most created connectors (62%) have moderate viability, with 13% showing high viability for official homologation. Regarding forest types, riparian forests are the most common in FSMSO (15%) and the most connected to official trails (17%). Our methodology supports integrated forest planning and trail design, enhancing socio-recreational opportunities, while emphasising the need for regulations addressing risks and challenges linked to promoting the public use of forests.</p>","PeriodicalId":543,"journal":{"name":"Environmental Management","volume":" ","pages":"2150-2168"},"PeriodicalIF":2.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12259729/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144482743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emulation or Degradation? Evaluating Forest Management Outcomes in Boreal Northeastern Ontario.","authors":"Jay R Malcolm, Julee J Boan, Justina C Ray","doi":"10.1007/s00267-025-02191-5","DOIUrl":"10.1007/s00267-025-02191-5","url":null,"abstract":"<p><p>Forest degradation has gained global attention for its role in exacerbating biodiversity loss and climate change, yet indicators, baselines, and thresholds of degradation remain under debate. Maintaining key forest characteristics within bounds of natural variability offers a strategy to sustain ecological integrity and to provide potential measures of degradation. We used forest inventories, satellite-derived information, and government planning guidelines to evaluate five potential indicators of forest degradation during 2012-2021 for public forests in boreal northeastern Ontario, Canada. We tested two contrasting hypotheses (natural disturbance emulation vs. timber maximization) by comparing observed values against those from two reference landscapes: one shaped by empirical estimates of natural fire disturbance regimes and one by forest management aimed at maximizing timber volumes. All indicators fell outside bounds of natural variability from natural landscapes and were more consistent with timber maximization. Specifically, compared to natural landscapes, some forest types were disturbed at substantially higher rates; the proportion of forest >100 years old was significantly lower (22.4% on average vs. 53.5% in a natural landscape); and modelled boreal caribou and American marten habitats were highly fragmented and substantially reduced (12% for boreal caribou and 36% for American marten vs. corresponding percentages of 73% vs. 76% in a natural landscape). Government planning targets for natural variability targets also were lower than, and did not overlap with, empirical estimates. Continued degradation of biodiversity and ecological services is likely unless management approaches are altered.</p>","PeriodicalId":543,"journal":{"name":"Environmental Management","volume":"75 8","pages":"1901-1922"},"PeriodicalIF":2.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12267323/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144641438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated trade-offs/synergies and interactions approach to quantifying the direct and indirect effects of environmental factors on ecosystem services.","authors":"Ying Huang, Ao Kang, Dewu Gao, Jin Li, Hao Zhang, Mengyang Yan, Xiaoyu Gan, Bo Zhou","doi":"10.1007/s00267-025-02171-9","DOIUrl":"10.1007/s00267-025-02171-9","url":null,"abstract":"<p><p>The driving mechanisms of ecosystem services (ESs) involve two aspects: the effects of environmental factors (e.g., precipitation and slope) on ESs and the effects of trade-offs/synergies on ESs. Clarifying the complex causal relationships between environmental factors and ESs is essential for decision-makers to formulate ES management. However, existing studies have focused more on identifying the main drivers of ESs without adequately exploring the direct and indirect effects of environmental factors on ESs, especially those based on the interactions between environmental factors and trade-offs/synergies on ESs. In this study, we proposed an integrated approach of trade-offs/synergies and interactions to quantify the direct and indirect effects of environmental factors on ESs by differentiating between the effects of trade-offs/synergies on ESs and the effects of environmental factors on ESs. Three typical ESs, net primary productivity (NPP), soil conservation (SC), and water yield (WY), were estimated in Sichuan Province from 2000-2020. The trade-offs/synergies between ES pairs were subsequently explored using correlation analysis and the geographically weighted regression (GWR) model. The interactions between environmental factors and ESs were verified and separated utilizing the Geodetector model and partial correlation analysis. Finally, the direct and indirect effects of environmental factors on ESs were measured through the bootstrap method. The results revealed that (1) from 2000-2020, three ESs exhibited significant spatial heterogeneity in Sichuan Province. (2) Complex trade-offs and synergies among these ESs were apparent at the provincial scale, characterized by distinct spatial heterogeneity. (3) DEM, temperature, precipitation, and relative humidity were the dominant factors affecting the spatial heterogeneity of ESs. Notably, the interactions involving environmental factors and ESs demonstrated more robust explanatory power for ESs and their trade-offs/synergies than individual drivers did. (4) DEM and temperature had significant direct and indirect effects on ESs when NPP and WY served as the mediating variables, and these mediating variables contributed significantly to the total effect. The integrated trade-offs/synergies and interactions approach deepens our understanding of ES mechanisms and provides a theoretical basis and reference for decision-making, rather than blindly pursuing the maximization of a particular service at the expense of others.</p>","PeriodicalId":543,"journal":{"name":"Environmental Management","volume":" ","pages":"1994-2009"},"PeriodicalIF":2.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using Wildlife-Vehicle Collision Records to Inform Wildlife Corridor Planning.","authors":"Amy Villamagna, Eric Laflamme, Hyun Joong Kim, Rebecca Martin","doi":"10.1007/s00267-025-02214-1","DOIUrl":"10.1007/s00267-025-02214-1","url":null,"abstract":"<p><p>Wildlife-vehicle collisions (WVCs) impose serious and increasing environmental, economic, and societal costs worldwide. Examining temporal and spatial patterns in WVCs is a critical piece of the wildlife connectivity puzzle that can help reduce the frequency and severity of collisions for humans and wildlife. We analyzed WVC records in New Hampshire (USA) between 2002 and 2019 to visualize spatiotemporal patterns, evaluate statistical predictors of WVCs, and to identify priority areas for mitigation efforts. More than 27,000 WVCs were reported between 2002 and 2019 throughout the entire state, averaging approximately 1500 WVCs per year. WVCs occurred on roads of all functional classes; notably, 33% occurred on \"local\" roads and at least 62% on roads with posted speeds greater than 40 mph. We calculated road-level metrics, including total WVCs and WVC density (i.e., WVCs per mile) over predefined, multiyear period and compared these metrics over space and time. Positive relationships were observed between WVCs and local road density, which corresponds to population centers within the state; however, only temporal factors were of practical significance in explaining WVC frequency. To inform statewide mitigation measures, we ranked roads based on WVCs per mile and total WVCs and identified the top 5% road for each during the most recent time periods.</p>","PeriodicalId":543,"journal":{"name":"Environmental Management","volume":" ","pages":"2103-2115"},"PeriodicalIF":2.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinformatic Analysis Reveals the Association of Human N-Terminal Acetyltransferase Complexes with Distinct Transcriptional and Post-Transcriptional Processes.","authors":"C Koufaris, C Demetriadou, V Nicolaidou, A Kirmizis","doi":"10.1007/s10528-024-10860-z","DOIUrl":"10.1007/s10528-024-10860-z","url":null,"abstract":"<p><p>N-terminal acetyltransferases (NAT) are the protein complexes that deposit the abundant N-terminal acetylation (Nt-Ac) on eukaryotic proteins, with seven human complexes currently identified. Despite the increasing recognition of their biological and clinical importance, NAT regulation remains elusive. In this study, we performed a bioinformatic investigation to identify transcriptional and post-transcriptional processes that could be involved in the regulation of human NAT complexes. First, co-expression analysis of independent transcriptomic datasets revealed divergent pathway associations for human NAT, which are potentially connected to their distinct cellular functions. One interesting connection uncovered was the coordinated regulation of the NatA and proteasomal genes in cancer and immune cells, confirmed by analysis of multiple datasets and in isolated primary T cells. Another distinctive association was of NAA40 (NatD) with DNA replication, in cancer and non-cancer settings. The link between NAA40 transcription and DNA replication is potentially mediated through E2F1, which we have experimentally shown to bind the promoter of this NAT. Second, the coupled examination of transcriptomic and proteomic datasets revealed a much greater intra-complex concordance of NAT subunits at the protein compared to the transcript level, indicating the predominance of post-transcriptional processes for achieving their coordination. In agreement with this concept, we also found that the effects of somatic copy number alterations affecting NAT genes are attenuated post-transcriptionally. In conclusion, this study provides novel insights into the regulation of human NAT complexes.</p>","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":"2987-3008"},"PeriodicalIF":2.1,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12271290/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141305130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of climate, water and peloid of the Pink Lagoon of Torrevieja and its possible use in thalassotherapy.","authors":"Francisco Maraver, Jose M Carbajo, Francisco Armijo, Lourdes Aguilera, Ana Isabel Martin-Megias, Miguel Angel Fernandez-Toran, María Virginia Fernandez-Gonzalez, María Lorena Vela","doi":"10.1007/s00484-025-02940-5","DOIUrl":"10.1007/s00484-025-02940-5","url":null,"abstract":"<p><p>The Pink Lagoon in Torrevieja is found in the Bajo Segura region of the province of Alicante (Spain). This exorheic lake of marine sedimentary origin was connected to the Mediterranean Sea up until Quaternary times. Today it acts as a salt flat, receiving water from its neighbouring Lagoon La Mata and brine from washing of the El Pinoso quarry. The purpose of the work is to study its environmental resources: the factors of the climate, the physicochemical properties and the composition of the waters, the thermal and textural characteristics of the natural peloid, and assess their possible use as thalassotherapeutic agents. The results obtained: marine climate; hypothermal water of strong mineralization (390 g/l), hypersaline, rich in magnesium sodium chloride and extremely hard; and lime muds of mineral sediment mainly organic with a low water content and scarce hardness and adhesiveness. They allow their application in thalassotherapy, obtaining the best results in musculoskeletal and skin disorders.</p>","PeriodicalId":588,"journal":{"name":"International Journal of Biometeorology","volume":" ","pages":"1943-1956"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the efficacy of peloid therapy and paraffin treatment in carpal tunnel syndrome.","authors":"Büşra Şirin Ahısha, Selma Akkaya Arı, Nurdan Paker, Nur Kesiktaş, Yasemin Barut","doi":"10.1007/s00484-025-02941-4","DOIUrl":"10.1007/s00484-025-02941-4","url":null,"abstract":"<p><p>Carpal tunnel syndrome (CTS) is a common neuropathy caused by median nerve compression at the wrist, managed with treatments like splinting, physical therapy, injections, and surgery. This study aimed to compare the effectiveness of paraffin and peloid therapies combined with night splint use in reducing symptom severity, improving functional status, and enhancing electrophysiological findings in patients with mild to moderate CTS.Sixty patients with mild to moderate CTS scheduled for paraffin or peloid therapy with night splint use were included. Symptom severity was assessed using the Visual Analog Scale (VAS) and the Boston Carpal Tunnel Syndrome Questionnaire's Symptom Severity Scale (BSSS). Functional status was evaluated with the Functional Status Scale (BFSS). Electrophysiological parameters, including distal sensory latency (DSL), distal motor latency (DML), sensory nerve action potential (SNAP), compound muscle action potential (CMAP), sensory conduction velocity (SCV), and motor conduction velocity (MCV), were measured via electromyography (EMG). Assessments were performed at baseline, and at the 4th and 12th weeks post-treatment. Both therapies significantly improved VAS and BSSS scores at the 4th and 12th weeks (p < 0.05). Functional status improved only in the paraffin group (p < 0.05). Electrophysiological improvements in DSL and DML were significant in the paraffin group (p < 0.05), while no significant changes were noted in the peloid group. Between-group comparisons showed significant differences in DSL, DML, and SCV in favor of paraffin (p < 0.05). Paraffin and peloid therapies with night splint use reduce CTS symptom severity, but paraffin is superior in improving function and nerve conduction.</p>","PeriodicalId":588,"journal":{"name":"International Journal of Biometeorology","volume":" ","pages":"1957-1967"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144053848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determining the most effective shade system based on physiological and productive performance of Bos taurus bulls in a subtropical climate.","authors":"Leonel Avendaño-Reyes, Ulises Macías-Cruz, M Ángeles López-Baca, Vielka J Castañeda-Bustos, Arnulfo Vicente-Pérez, Miguel Á Gastélum-Delgado, José A Aguilar-Quiñonez, Peter Robinson, José A Roque-Jiménez, Miguel Mellado, Jorge H Siller","doi":"10.1007/s00484-025-02945-0","DOIUrl":"10.1007/s00484-025-02945-0","url":null,"abstract":"<p><p>The aim was to identify the best of four shade systems based on the physiological and productive responses of Bos taurus bulls in a subtropical climate. A total of 804 bulls from Bos taurus, Bos indicus, and their crosses were randomly allotted to 12 pens (n = 67 bulls/pen), which were randomly assigned to one of four treatments (3 pens/treatment): (1) Conventional shade (CSS), (2) double shade (DSH), (3) dome without fans (DNF), and (4) dome with fans (DWF). Five Bos taurus bulls per pen were randomly selected for physiological evaluation and collection of blood samples. Productive responses included feedlot performance, carcass traits and meat quality. The climate was judged as \"moderate heat stress\". Morning respiration frequency was lower in CSS and DWF bulls than in DNF and DSH bulls, but was the lowest in DWF bulls by the afternoon. Bulls under DWF had the lowest surface temperatures of head, loin, shoulder, rump and belly, as well as the highest triiodothyronine concentrations. Glucose concentration tended to be the lowest in DNF bulls, while chloride concentration tended to be higher in CSS bulls than in DSH and DNF bulls. The DNF bulls had the highest feed intake, while DWF and DSH bulls had better feed efficiency than CSS bulls. Marbling was higher in CSS and DSH than in DNF bulls, and meat from CSS bulls had less lightness than that from DNF and DWF bulls. Therefore, the most effective shade for Bos taurus bulls under moderate heat stress is the dome with fans as it improved thermoregulation, metabolic activity and feed efficiency.</p>","PeriodicalId":588,"journal":{"name":"International Journal of Biometeorology","volume":" ","pages":"1987-1998"},"PeriodicalIF":3.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}