Circ_0000758 Facilitates Bladder Cancer Cell Growth, Migration and Angiogenesis Via Severing as miR-1236-3p Sponge.

IF 2.1 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Genetics Pub Date : 2024-06-13 DOI:10.1007/s10528-024-10855-w

Qiang Chi, Zhiyong Wang, Hui Xu, Hongyang Li, Dianbin Song

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引用次数: 0

Abstract

Circular RNA (circRNA) has been reported to regulate the development of bladder cancer (BCa). However, the role of circ_0000758 in BCa progression is unknown. Circ_0000758 and miR-1236-3p expression, as well as ZEB2 mRNA expression were determined by qRT-PCR. BCa cell biological functions were determined by MTT assay, EdU assay, flow cytometry, wound healing assay and tube formation assay. Protein expression was detected by western blot analysis. RNA pull-down assay and dual-luciferase reporter assay were used to confirm RNA interaction. Xenograft mice models were constructed to assess the effect of circ_0000758 on BCa tumor growth. Circ_0000758 had increased expression in BCa tissues and cells. Circ_0000758 silencing could inhibit BCa cell growth, migration, angiogenesis in vitro, and tumor growth in vivo. Circ_0000758 served as a molecular sponge for miR-1236-3p, and miR-1236-3p inhibitor reversed circ_0000758 knockdown-mediated the inhibition effect on BCa cell progression. ZEB2 was targeted by miR-1236-3p, and its overexpression also revoked the suppressive effect of miR-1236-3p on BCa cell growth, migration, and angiogenesis. Besides, circ_0000758 knockdown also restrained BCa tumor growth. Circ_0000758 might promote BCa cell growth, migration, and angiogenesis by regulating the miR-1236-3p/ZEB2 axis.

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Circ_0000758 作为 miR-1236-3p 海绵可促进膀胱癌细胞的生长、迁移和血管生成。

据报道，环状 RNA（circRNA）可调控膀胱癌（BCa）的发展。然而，circ_0000758在膀胱癌进展中的作用尚不清楚。通过 qRT-PCR 方法测定了 circ_0000758 和 miR-1236-3p 的表达以及 ZEB2 mRNA 的表达。通过 MTT 试验、EdU 试验、流式细胞术、伤口愈合试验和管形成试验测定 BCa 细胞的生物学功能。蛋白表达通过 Western 印迹分析检测。采用 RNA 拉取试验和双荧光素酶报告试验确认 RNA 相互作用。构建了异种移植小鼠模型，以评估 circ_0000758 对 BCa 肿瘤生长的影响。Circ_0000758在BCa组织和细胞中的表达增加。沉默Circ_0000758可抑制体外BCa细胞生长、迁移、血管生成和体内肿瘤生长。Circ_0000758是miR-1236-3p的分子海绵，miR-1236-3p抑制剂逆转了circ_0000758敲除介导的对BCa细胞进展的抑制作用。ZEB2是miR-1236-3p的靶标，过表达ZEB2也会逆转miR-1236-3p对BCa细胞生长、迁移和血管生成的抑制作用。此外，circ_0000758的敲除也抑制了BCa肿瘤的生长。Circ_0000758可能通过调节miR-1236-3p/ZEB2轴促进BCa细胞的生长、迁移和血管生成。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochemical Genetics 生物-生化与分子生物学

CiteScore

3.90

自引率

0.00%

发文量

133

审稿时长

4.8 months

期刊介绍： Biochemical Genetics welcomes original manuscripts that address and test clear scientific hypotheses, are directed to a broad scientific audience, and clearly contribute to the advancement of the field through the use of sound sampling or experimental design, reliable analytical methodologies and robust statistical analyses. Although studies focusing on particular regions and target organisms are welcome, it is not the journal’s goal to publish essentially descriptive studies that provide results with narrow applicability, or are based on very small samples or pseudoreplication. Rather, Biochemical Genetics welcomes review articles that go beyond summarizing previous publications and create added value through the systematic analysis and critique of the current state of knowledge or by conducting meta-analyses. Methodological articles are also within the scope of Biological Genetics, particularly when new laboratory techniques or computational approaches are fully described and thoroughly compared with the existing benchmark methods. Biochemical Genetics welcomes articles on the following topics: Genomics; Proteomics; Population genetics; Phylogenetics; Metagenomics; Microbial genetics; Genetics and evolution of wild and cultivated plants; Animal genetics and evolution; Human genetics and evolution; Genetic disorders; Genetic markers of diseases; Gene technology and therapy; Experimental and analytical methods; Statistical and computational methods.