Cellular Microbiology最新文献

{"title":"Inhibition of Homeobox D10 Alleviates Acute Kidney Injury by Upregulating PI3K/AKT Signaling Proteins","authors":"Siqi Liu,&nbsp;Huixin Sun,&nbsp;Jingjie Guo,&nbsp;Linlin Ma","doi":"10.1155/2022/2955546","DOIUrl":"10.1155/2022/2955546","url":null,"abstract":"<div>\u0000 <p><i>Purpose</i>. To observe the protective effect of homeobox D10 (HOXD10) on acute kidney injury (AKI) by regulating PI3K/AKT signaling pathway is the purpose of this study.<i>Methods</i>. 30 rats were randomly divided into three groups: blank control group, model group, and HOXD10 interference group. The kidney function indexes, HOXD10 protein expression, histopathological features, tubulointerstitial injury, and PI3K and AKT protein expression levels of the three groups were analyzed. <i>Results</i>. Compared with the blank control group, the kidney weight, BUN and SCr in model group increased significantly, and TIL score was higher (<i>P</i> &gt; 0.05). The expression of HOXD10 in model group and HOXD10 interference group were higher than blank control group, and the expression of HOXD10 in HOXD10 interference group was lower than model group (<i>P</i> &lt; 0.05). After we administered HOXD10 blocker to AKI rats, pathological sections by HE staining showed that the kidney tissue damage was significantly reduced compared with the model group, and the expression levels of BUN and SCr in kidney tissue decreased, and the TIL score decreased. The expression of p-PI3K and p-AKT decreased after kidney injury. Compared with the model group, the phosphorylation levels of PI3K and AKT in HOXD10 interference group were significantly increased (<i>P</i> &lt; 0.05). <i>Conclusion</i>. Downregulation of HOXD10 can play a protective role on AKI by activating PI3K/AKT signaling pathway, which can reduce tubulointerstitial injury and improve kidney function.</p>\u0000 </div>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2022 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2022/2955546","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48631848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Guanylate-Binding Protein 1 Regulates Infection-Induced Autophagy through TBK1 Phosphorylation","authors":"Miyako Hikichi,&nbsp;Hirotaka Toh,&nbsp;Atsuko Minowa-Nozawa,&nbsp;Takashi Nozawa,&nbsp;Ichiro Nakagawa","doi":"10.1155/2022/8612113","DOIUrl":"10.1155/2022/8612113","url":null,"abstract":"<div>\u0000 <p>Invading bacteria can be degraded by selective autophagy, known as xenophagy. Recent studies have shown that the recruitment of autophagy adaptor proteins such as p62 to bacteria and its regulation by activated TANK-binding kinase 1 (TBK1) are required to overcome bacterial infection. However, the detailed molecular mechanisms behind this are not yet fully understood. Here, we show that the human guanylate-binding protein (GBP) family, especially GBP1, directs xenophagy against invading Group A <i>Streptococcus</i> (GAS) by promoting TBK1 phosphorylation. GBP1 exhibits a GAS-surrounding localization response to bacterially caused membrane damage mediated by the membrane damage sensor galectin-3. We found that GBP1 knockout attenuated TBK1 activation, followed by reduced p62 recruitment and lower bactericidal activity by xenophagy. Furthermore, GBP1-TBK1 interaction was detected by immunoprecipitation. Our findings collectively indicate that GBP1 contributes to GAS-targeted autophagy initiated by membrane damage detection by galectin-3 via TBK1 phosphorylation.</p>\u0000 </div>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2022 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2022-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2022/8612113","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42225872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mathematical Analysis of the Healthcare Treatment of 215 Patients with Coronary Heart Disease","authors":"Meiyi Tao,&nbsp;Shengli Sun,&nbsp;Yuelan Qin,&nbsp;Juan Wu,&nbsp;Yimin Cai,&nbsp;Dandan Li,&nbsp;Ke Tang,&nbsp;Ling Li,&nbsp;Shuang Wu","doi":"10.1155/2022/2134472","DOIUrl":"10.1155/2022/2134472","url":null,"abstract":"<div>\u0000 <p>The main risk factors for CHD and the comorbidity include hyperlipidemia (HL), hypertension, smoking, dietary factors, and genetic factors. In this work, 215 patients with coronary heart disease, including 128 males and 87 females, were analyzed for a better understanding of the related clinical pharmacology. Nonparametric test, analysis of variance, chi-square test, correlation analysis, and other methods were used to sort out the data. From the analysis, there are significant differences in age among different gender samples. The incidence of coronary heart disease in men is five years younger than that in women. The sample pairs from different regions showed differences in the presence of family history of diabetes, indicating that a series of patients in some regions concentrated on the disease status of family history of diabetes. Age has a significant positive effect on cardiac functional classification. The older you are, the larger the cardiac functional classification is and the worse the cardiac function is. Age was negatively correlated with VTE score, diastolic blood pressure, CAR, TG, neutrophil, and TC. The older you are, the lower these six values are. Samples of different types of CHD showed significant differences in the presence of comorbidity and family history of CHD. The most significant are unstable angina pectoris and ischemic cardiomyopathy. Samples of different CHD types showed significant effects on VTE score, creatine kinase, low-density lipoprotein cholesterol (LDL⁃C), and lactate dehydrogenase. The highest lactate dehydrogenase is ischemic cardiomyopathy. The highest LDL cholesterol is ST-segment elevation angina. The highest creatine kinase is ischemic cardiomyopathy. The VTE score was the highest for ischemic cardiomyopathy, followed by non-ST-segment elevation angina. Samples taken with or without lipid-lowering drugs showed significant differences in lactate dehydrogenase, creatinine, and TC. There was a significant positive correlation between VTE scores and lactate dehydrogenase, myoglobin, and creatine kinase. High VTE score indicates high lactate dehydrogenase, myoglobin, and creatine kinase. TC has a significant positive correlation with HDL⁃C and TG, respectively. Higher TC values indicate higher HDL⁃C and TG values.</p>\u0000 </div>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2022 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2022-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2022/2134472","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138518992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Symbiosis with Dinoflagellates Alters Cnidarian Cell-Cycle Gene Expression","authors":"Lucy M. Gorman,&nbsp;Migle K. Konciute,&nbsp;Guoxin Cui,&nbsp;Clinton A. Oakley,&nbsp;Arthur R. Grossman,&nbsp;Virginia M. Weis,&nbsp;Manuel Aranda,&nbsp;Simon K. Davy","doi":"10.1155/2022/3330160","DOIUrl":"10.1155/2022/3330160","url":null,"abstract":"<div>\u0000 <p>In the cnidarian-dinoflagellate symbiosis, hosts show altered expression of genes involved in growth and proliferation when in the symbiotic state, but little is known about the molecular mechanisms that underlie the host’s altered growth rate. Using tissue-specific transcriptomics, we determined how symbiosis affects expression of cell cycle-associated genes, in the model symbiotic cnidarian <i>Exaiptasia diaphana</i> (Aiptasia). The presence of symbionts within the gastrodermis elicited cell-cycle arrest in the G₁ phase in a larger proportion of host cells compared with the aposymbiotic gastrodermis. The symbiotic gastrodermis also showed a reduction in the amount of cells synthesizing their DNA and progressing through mitosis when compared with the aposymbiotic gastrodermis. Host apoptotic inhibitors (Mdm2) were elevated, while host apoptotic sensitizers (c-Myc) were depressed, in the symbiotic gastrodermis when compared with the aposymbiotic gastrodermis and epidermis of symbiotic anemones, respectively. This indicates that the presence of symbionts negatively regulates host apoptosis, possibly contributing to their persistence within the host. Transcripts (ATM/ATR) associated with DNA damage were also downregulated in symbiotic gastrodermal tissues. In epidermal cells, a single gene (Mob1) required for mitotic completion was upregulated in symbiotic compared with aposymbiotic anemones, suggesting that the presence of symbionts in the gastrodermis stimulates host cell division in the epidermis. To further corroborate this hypothesis, we performed microscopic analysis using an S-phase indicator (EdU), allowing us to evaluate cell cycling in host cells. Our results confirmed that there were significantly more proliferating host cells in both the gastrodermis and epidermis in the symbiotic state compared with the aposymbiotic state. Furthermore, when comparing between tissue layers in the presence of symbionts, the epidermis had significantly more proliferating host cells than the symbiont-containing gastrodermis. These results contribute to our understanding of the influence of symbionts on the mechanisms of cnidarian cell proliferation and mechanisms associated with symbiont maintenance.</p>\u0000 </div>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2022 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2022-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2022/3330160","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44894837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LncRNA LINC01116 Regulates the Proliferation, Migration, and Invasion of Cervical Cancer Cells by Targeting miR-744-5p","authors":"Jing Wang,&nbsp;Lei Yue,&nbsp;Ye Shen","doi":"10.1155/2022/2615523","DOIUrl":"10.1155/2022/2615523","url":null,"abstract":"<div>\u0000 <p><i>Objective</i>. To investigate the effects and potential molecular mechanisms of LncRNA LINC01116 on proliferation, migration, and invasion of cervical cancer cells. <i>Method</i>(<i>s</i>). The content of miR-744-5p and LINC01116 in cervical cancer cells HeLa, SiHa, and C33a was detected by RT-PCR, the proliferative activity and clone number of SiHa cells were determined by MTT and clone formation assay, the number of invaded and migrated cells was determined by Transwell assay, the expressions of Cyclin D1 and MMP-2 in cells were detected by Western blot, and the activity of luciferase detected by dual-luciferase reporting system verified the regulatory relationship between LINC01116 and miR-744-5p. <i>Result</i>(<i>s</i>). Compared with human normal cervical epithelial cells Ect1/E6E7, the content of LINC01116 in cervical cancer cells HeLa, SiHa, and C33a was increased significantly [(1.04 ± 0.12) vs. (3.34 ± 0.38)/(4.48 ± 0.45)/(3.59 ± 0.45)] (<i>P</i> &lt; 0.05), the content of miR-744-5p was decreased significantly [(0.98 ± 0.09) vs. (0.39 ± 0.04)/(0.34 ± 0.05)/(0.41 ± 0.05)] (<i>P</i> &lt; 0.05). Silencing LINC01116 could inhibit the protein expression of Cyclin D1 [(0.68 ± 0.06) vs. (0.31 ± 0.04)] and MMP-2 [(0.74 ± 0.08) vs. (0.34 ± 0.05)] in SiHa cells, and inhibition of cell proliferation [(100.98 ± 7.89)% vs. (67.26 ± 6.14)%], clone formation ability [(180.31 ± 11.34) vs. (53.56 ± 6.18)], migration [(234.67 ± 15.31) vs. (98.46 ± 7.46)], and invasion [(134.83 ± 9.12) vs. (62.44 ± 5.34)]. LINC01116 targeted and negatively regulates the expression of miR-744-5p. Overexpression of miR-744-5p could inhibit the proliferation of cervical cancer SiHa cells [(101.64 ± 4.89)% vs. (62.34 ± 5.73)%], clone formation ability [(179.24 ± 12.34) vs. (47.67 ± 5.18)], migration [(241.06 ± 16.64) vs. (84.52 ± 7.57)], and invasion [(127.92 ± 8.16) vs. (65.24 ± 7.36)]. Inhibition of miR-744-5p reversed the effects of silencing LINC01116 on SiHa cell proliferation [(102.46 ± 7.64)% vs. (146.72 ± 12.23)%], clone formation ability [(47.64 ± 6.20) vs. (104.26 ± 8.64)], migration [(81.52 ± 7.42) vs. (153.64 ± 12.57)], and invasion [(69.12 ± 5.56) vs (94.31 ± 7.44)]. <i>Conclusion</i>(<i>s</i>). LncRNA LINC01116 inhibits proliferation, migration, and invasion of SiHa cells by targeting miR-744-5p, and LINC01116 is a potential molecular target for cervical cancer.</p>\u0000 </div>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2022 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2022-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2022/2615523","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42995469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Gastric Diseases and Their Symptoms Based on Indexes of Pepsinogen I (PGI) and Pepsinogen II (PGII): Take 1106 Patients as Samples","authors":"Kuanpeng Guo,&nbsp;Zhengqiu Li,&nbsp;Bibo Qiu,&nbsp;Bin Hu,&nbsp;Suwu Yi","doi":"10.1155/2022/8393351","DOIUrl":"10.1155/2022/8393351","url":null,"abstract":"<div>\u0000 <p>In this study, preoperative analysis of 1106 gastropathy patients with abdominal pain, vomiting, dyspepsia, and other symptoms was conducted. Independent sample <i>t</i>-test and correlation analysis and other ways were used for data cleansing and analysis. Findings were as follows: (1) Samples of different genders showed significance in PGI and PGII. The PGI and PGII values of women were significantly lower than those of men. (2) Age showed a significant positive correlation with PGI and PGII, which indicates that as the age increases, the PGI and PGII values become higher. (3) There was a significant negative correlation between age and abdominal pain. This signified that the younger the patient is, the more likely they will suffer abdominal pain. (4) PGI displayed a positive correlation with abdominal pain in the digestive tract (dyspepsia, gastrointestinal ulcers, gastrointestinal bleeding, etc.). It indicated that the higher the PGI value is, the more likely the patients will suffer abdominal pain and gastrointestinal diseases (dyspepsia, gastrointestinal ulcer, gastrointestinal hemorrhage, etc.). (5) PGII displayed a significant positive correlation with gastrointestinal diseases (dyspepsia, gastrointestinal ulcer, gastrointestinal hemorrhage, etc.) and a negative correlation with gastropathy (acute gastritis, chronic superficial gastritis, gastric ulcer, etc.). It indicated that the higher the value of PGII is, the more likely the patients will suffer symptoms of gastrointestinal diseases (dyspepsia, gastrointestinal ulcer, gastrointestinal hemorrhage, etc.), while less likely the patients will suffer gastropathy (acute gastritis, chronic superficial gastritis, gastric ulcer, etc.).</p>\u0000 </div>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2022 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2022-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2022/8393351","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47490379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Effects of PTEN-Mediated TGF-β/Smad2 Pathway on Osteogenic Differentiation in Osteoporotic Tibial Fracture Rats and Bone Marrow Mesenchymal Stem Cell under Tension","authors":"Shiyong Ling,&nbsp;Chen Yan,&nbsp;Kai Huang,&nbsp;Bo Lv,&nbsp;Hua Wang,&nbsp;Xiaoyan Wang,&nbsp;Jun Chen,&nbsp;Jingchuan Sun","doi":"10.1155/2022/1004203","DOIUrl":"10.1155/2022/1004203","url":null,"abstract":"<div>\u0000 <p><i>Purpose</i>. To discuss effects of phosphatase and tensin homolog protein (PTEN)-mediated transforming growth factor-<i>β</i> (TGF-<i>β</i>)/Smad homologue 2 (Smad2) pathway on osteogenic differentiation in osteoporotic (OP) tibial fracture rats and bone marrow mesenchymal stem cell (BMSC) under tension. <i>Methods</i>. A tibial fracture model was established. The rats were divided into sham-operated group and model group, and tibia tissue was collected. Purchase well-grown cultured rat BMSC, and use the Flexercell in vitro cell mechanics loading device to apply tension. The expression of PTEN was detected by qRT-PCR. After the BMSCs were transfected with si-PTEN and oe-PTEN, the force was applied to detect cell differentiation. The expression of TGF-<i>β</i>/Smad2 protein was detected by Western blot. The formation of calcium nodules in BMSC was detected by alkaline phosphatase (ALP) staining and alizarin red (AR) staining. <i>Results</i>. The expression of PTEN was higher in the model group and tension MSC group, and the expression of TGF-<i>β</i> and Smad2 protein was lower. The expression of TGF-<i>β</i> and Smad2 protein in oe-PTEN group was lower than the oe-NC group and control group. The expression of TGF-<i>β</i> and Smad2 protein in si-PTEN group was higher than the si-NC group and control group. The results of ALP staining and AR staining also confirmed the above results. <i>Conclusion</i>. PTEN-mediated TGF-<i>β</i>/Smad2 pathway may play a key role in the osteogenic differentiation of OP tibial fracture rats. Downregulation of PTEN and upregulation of TGF-<i>β</i>/Smad2 signal can promote the osteogenic differentiation of BMSC under tension.</p>\u0000 </div>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2022 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2022-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2022/1004203","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45490596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the Antifatigue Effect of Compound Amino Acid Capsules","authors":"Wen Huang,&nbsp;Huaqiang Hui,&nbsp;Jiping Xu,&nbsp;Honglei Guo,&nbsp;Yunfeng Wang,&nbsp;Wei Zhu","doi":"10.1155/2022/6593811","DOIUrl":"10.1155/2022/6593811","url":null,"abstract":"<div>\u0000 <p>Supplementing amino acids was proven to relieve fatigue caused by exercise. This study explored the antifatigue effects of compound amino acid capsules (CAAC) on rats undergoing the forced swimming test (FST). CAAC augmented the endurance of FST in rats and alleviated the damage of skeletal muscle tissue and reduced the content of biochemical indicators in the serum. Furthermore, CAAC prevented skeletal muscle dysfunction in FST rats by modulating inflammation and oxidation reactions. After the treatment with CAAC, apoptosis and apoptosis-related protein and p-p65 were weakened, while the levels of SIRT1 and SIRT1/PGC-1<i>α</i>/Nrf2 pathway-related proteins were enhanced. The antifatigue properties of CAAC were associated with its antioxidant and anti-inflammatory capabilities, which were realized by activating the SIRT1/PGC-1<i>α</i>/Nrf2 pathway.</p>\u0000 </div>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2022 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2022-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2022/6593811","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47700500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial Atlas of Mouse Gut Microbiota","authors":"Mengqi Chu,&nbsp;Xiaobo Zhang","doi":"10.1155/2022/5968814","DOIUrl":"10.1155/2022/5968814","url":null,"abstract":"<div>\u0000 <p><i>Background</i>. Mouse model is one of the most widely used animal models for exploring the roles of human gut microbiota, a complex system involving in human immunity and metabolism. However, the structure of mouse gut bacterial community has not been explored at a large scale. To address this concern, the diversity and composition of the gut bacteria of 600 mice were characterized in this study. <i>Results</i>. The results showed that the bacteria belonging to 8 genera were found in the gut microbiota of all mouse individuals, indicating that the 8 bacteria were the core bacteria of mouse gut microbiota. The dominant genera of the mouse gut bacteria contained 15 bacterial genera. It was found that the bacteria in the gut microbiota were mainly involved in host’s metabolisms via the collaborations between the gut bacteria. The further analysis demonstrated that the composition of mouse gut microbiota was similar to that of human gut microbiota. <i>Conclusion</i>. Our study presented a bacterial atlas of mouse gut microbiota, providing a solid basis for investing the bacterial communities of mouse gut microbiota.</p>\u0000 </div>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2022 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2022-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2022/5968814","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138518991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chlamydia pneumoniae Interferes with Macrophage Differentiation and Cell Cycle Regulation to Promote Its Replication","authors":"Eveliina Taavitsainen-Wahlroos,&nbsp;Ilkka Miettinen,&nbsp;Maarit Ylätalo,&nbsp;Inés Reigada,&nbsp;Kirsi Savijoki,&nbsp;Tuula Anneli Nyman,&nbsp;Leena Hanski","doi":"10.1155/2022/9854449","DOIUrl":"10.1155/2022/9854449","url":null,"abstract":"<div>\u0000 <p><i>Chlamydia pneumoniae</i> is a ubiquitous intracellular bacterium which infects humans via the respiratory route. The tendency of <i>C. pneumoniae</i> to persist in monocytes and macrophages is well known, but the underlying host-chlamydial interactions remain elusive. In this work, we have described changes in macrophage intracellular signaling pathways induced by <i>C. pneumoniae</i> infection. Label-free quantitative proteome analysis and pathway analysis tools were used to identify changes in human THP-1-derived macrophages upon <i>C. pneumoniae</i> CV6 infection. At 48-h postinfection, pathways associated to nuclear factor <i>κ</i>B (NF-<i>κ</i>B) regulation were stressed, while negative regulation on cell cycle control was prominent at both 48 h and 72 h. Upregulation of S100A8 and S100A9 calcium binding proteins, osteopontin, and purine nucleoside hydrolase, laccase domain containing protein 1 (LACC1) underlined the proinflammatory consequences of the infection, while elevated NF-<i>κ</i>B2 levels in infected macrophages indicates interaction with the noncanonical NF-<i>κ</i>B pathway. Infection-induced alteration of cell cycle control was obvious by the downregulation of mini chromosome maintenance (MCM) proteins MCM2-7, and the significance of host cell cycle regulation for <i>C. pneumoniae</i> replication was demonstrated by the ability of a cyclin-dependent kinase (CDK) 4/6 inhibitor Palbociclib to promote <i>C. pneumoniae</i> replication and infectious progeny production. The infection was found to suppress retinoblastoma expression in the macrophages in both protein and mRNA levels, and this change was reverted by treatment with a histone deacetylase inhibitor. The epigenetic suppression of retinoblastoma, along with upregulation of S100A8 and S100A9, indicate host cell changes associated with myeloid-derived suppressor cell (MDSC) phenotype.</p>\u0000 </div>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2022 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2022-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2022/9854449","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"93577916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}