健康大鼠肝脏微生物组:肝细胞的隐性宿主

IF 2.6 2区 生物学 Q3 CELL BIOLOGY
Xiaowei Sun, Hua Zhang, Xiao Zhang, Pengfei Xin, Xue Gao, Hong Rui Li, Cai Yun Zhou, Wen Min Gao, Xuan Xuan Kou, Jian Gang Zhang
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引用次数: 1

摘要

最近对人类的肿瘤和组织微生物群进行了研究。肠道通透性是组织细菌阳性检测的可能来源。在此,我们报告在健康大鼠的肝细胞中检测到高丰度的微生物群,并且在一定程度上与肠道微生物群共享。我们评估了雄性Sprague-Dawley(SD)大鼠的16S核糖体核糖核酸(rRNA)基因。通过门静脉抽血处死大鼠后,我们从其回肠、结肠内容物和肝组织中提取总脱氧核糖核酸(DNA)。通过聚合酶链式反应(PCR)扩增16S rRNA基因的V3–V4区域,并使用Illumina HiSeq 2500平台进行测序。序列由SILVA数据库进行分类分配。我们还使用免疫荧光(IF)和蛋白质印迹原位检测细菌脂多糖(LPS)和脂磷壁酸(LTA),并使用荧光原位杂交(FISH)检测16S rRNA基因。在6只大鼠的肝脏中,我们检测到54867.50±6450.03个16S rRNA基因的有效标签,并将其聚类为1003种操作分类单元(OTU;805.67±70.14729–893)。大鼠表现出细菌丰富度、丰度和均匀度的保守性。在肝细胞核中检测到LPS和16S rRNA基因。注释细菌基因组的主要功能组成与代谢相关(79.92±0.24%)。革兰氏阴性大约是革兰氏阳性的1.6倍。肝脏微生物组与小肠和大肠共享,但表现出比肠道微生物组更高的丰富度和均匀性,β-多样性结果显示,肝脏微生物组表现出比小肠和大肠显著更高的相似性(P<0.05)。我们的研究结果表明,肝脏微生物组中的细菌是健康大鼠肝脏中隐藏的细胞内居民。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Liver Microbiome in Healthy Rats: The Hidden Inhabitants of Hepatocytes
The tumor and tissue microbiota of human beings have recently been investigated. Gut permeability is known as a possible resource for the positive detection of tissue bacteria. Herein, we report that microbiota were detected in high abundance in the hepatocytes of healthy rats and that they were shared with the gut microbiota to an extent. We assessed male Sprague Dawley (SD) rats for the 16S ribosomal ribonucleic acid (rRNA) gene. After the rats were sacrificed by blood drainage from the portal vein, we extracted total deoxyribonucleic acid (DNA) from their ileal and colonic contents and liver tissues. The V3–V4 region of the 16S rRNA gene was amplified by polymerase chain reaction (PCR) and sequenced using an Illumina HiSeq 2500 platform. Sequences were assigned taxonomically by the SILVA database. We also detected bacterial lipopolysaccharide (LPS) and lipoteichoic acid (LTA) in situ using immunofluorescence (IF) and western blotting and the 16S rRNA gene using fluorescent in situ hybridization (FISH). In the livers of six rats, we detected 54,867.50 ± 6450.03 effective tags of the 16S rRNA gene and clustered them into 1003 kinds of operational taxonomic units (OTUs; 805.67 ± 70.14 , 729–893). Rats showed conservation of bacterial richness, abundance, and evenness. LPS and the 16S rRNA gene were detected in the nuclei of hepatocytes. The main function composition of the genomes of annotated bacteria was correlated with metabolism ( 79.92 ± 0.24 % ). Gram negativity was about 1.6 times higher than gram positivity. The liver microbiome was shared with both the small and large intestines but showed significantly higher richness and evenness than the gut microbiome, and the β-diversity results showed that the liver microbiome exhibited significantly higher similarity than the small and large intestines ( P < 0.05 ). Our results suggest that the bacteria in the liver microbiome are hidden intracellular inhabitants in healthy rat livers.
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来源期刊
Cellular Microbiology
Cellular Microbiology 生物-微生物学
CiteScore
9.70
自引率
0.00%
发文量
26
审稿时长
3 months
期刊介绍: Cellular Microbiology aims to publish outstanding contributions to the understanding of interactions between microbes, prokaryotes and eukaryotes, and their host in the context of pathogenic or mutualistic relationships, including co-infections and microbiota. We welcome studies on single cells, animals and plants, and encourage the use of model hosts and organoid cultures. Submission on cell and molecular biological aspects of microbes, such as their intracellular organization or the establishment and maintenance of their architecture in relation to virulence and pathogenicity are also encouraged. Contributions must provide mechanistic insights supported by quantitative data obtained through imaging, cellular, biochemical, structural or genetic approaches.
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