Cellular Microbiology最新文献

{"title":"Staphylococcus aureus Cell Envelope as a Target of (-)-Globulol","authors":"Ruixiu Liu,&nbsp;Hui Yang,&nbsp;Weijin Qi,&nbsp;Hongying Wang,&nbsp;Xianfang He,&nbsp;Qiong Yi,&nbsp;Lu Wang","doi":"10.1155/cmi/8895641","DOIUrl":"https://doi.org/10.1155/cmi/8895641","url":null,"abstract":"<p>(-)-Globulol is a sesquiterpenoid with a variety of biological activities such as antifungal, antiparasitic, and anticomplexinase. However, the potential antibacterial properties of (-)-globulol remain uncertain. Here, the antibacterial effects of (-)-globulol against <i>Staphylococcus aureus</i> (<i>S. aureus</i>) were evaluated by assays of bacterial growth profiles, cell surface hydrophobicity (CSH), and growth phenomenon of bacteria in the liquid culture medium, cellular microstructure, permeability of cell envelopes, and liquid chromatography–mass spectrometer (LC-MS) nontargeted metabolomics. The result of the bacterial growth assay showed that the minimum inhibitory concentration (MIC) value of (-)-globulol against <i>S. aureus</i> was 7.81 <i>μ</i>g/mL. When exposed to (-)-globulol, dose-dependent aggregation of <i>S. aureus</i> was observed, triggered by increased CSH, disruption of the cell envelope, and cell wall integrity. Metabolomic analysis exhibited fluctuations in metabolites associated with <i>S. aureus</i> cell membranes, such as upregulation of <i>N</i>-acetyl glutamic acid and biotin and downregulation of cytidine diphosphoglycerol and <i>D</i>-glucaric acid, indicating their inhibition. Simultaneously, cell wall synthesis was also disrupted by the upregulation of <i>L</i>-tryptophan and palmitoleic acid. In summary, we confirm that cell membrane disruption by (-)-globulol plays a key role in its antimicrobial activity.</p>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2026 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/cmi/8895641","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147715097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: Haemolysin Sph2 of Leptospira Interrogans Induces Cell Apoptosis via Intracellular Reactive Oxygen Species Elevation and Mitochondrial Membrane Injury","authors":"Cellular Microbiology","doi":"10.1155/cmi/9812897","DOIUrl":"https://doi.org/10.1155/cmi/9812897","url":null,"abstract":"<p>RETRACTION: R. Che, S. Ding, Q. Zhang, W. Yang, J. Yan, X. Lin, “Haemolysin Sph2 of <i>Leptospira interrogans</i> induces cell apoptosis via intracellular reactive oxygen species elevation and mitochondrial membrane injury,” <i>Cellular Microbiology</i>. 2019; 21:e12959. https://doi.org/10.1111/cmi.12959</p><p>The authors were unresponsive when asked to provide a response to the concerns. Following an assessment, it has been concluded that the results and conclusions can no longer be considered reliable, and the article is therefore retracted.</p><p>The authors did not respond to indicate their agreement to the retraction.</p>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2026 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/cmi/9812897","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147715091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ADMET Study of Synthetic Chalcones and Their In Vitro Inhibitory Effect on the Morphological Transition of Candida albicans and Candida tropicalis","authors":"Antonia Thassya Lucas dos Santos,&nbsp;Maria Elenilda Paulino da Silva,&nbsp;Joara Nályda Pereira Carneiro,&nbsp;Francildo dos Santos Silva,&nbsp;Maria Lucilene Queiroz da Silva,&nbsp;Victor Juno Alencar Fonseca,&nbsp;José Bezerra de Araújo-Neto,&nbsp;Henrique Douglas Melo Coutinho,&nbsp;Hélcio Silva dos Santos,&nbsp;Francisco Rogenio da Silva Mendes,&nbsp;Carolina Bandeira Domiciano,&nbsp;Débora Lima Sales,&nbsp;Radosław Kowalski,&nbsp;Grażyna Kowalska,&nbsp;Rafal Rowinski,&nbsp;Maria Audilene de Freitas,&nbsp;Maria Flaviana Bezerra Morais-Braga","doi":"10.1155/cmi/6676802","DOIUrl":"https://doi.org/10.1155/cmi/6676802","url":null,"abstract":"<p>The morphological transition of <i>Candida</i> spp. is a key virulence factor that enables these fungi to cause infections in humans. This ability to shift between yeast-like, hyphal, and pseudohyphal forms allows <i>Candida</i> species to adapt to diverse environments and hostile conditions, playing a critical role in pathogenicity. In this study, we investigated the effects of synthetic chalcones on the morphological transition of <i>Candida albicans</i> and <i>Candida tropicalis</i>, alongside an in silico evaluation of the compounds’ pharmacokinetic properties, including absorption, distribution, metabolism, excretion, and toxicity (ADMET). To assess the inhibitory effects of chalcones and fluconazole on morphological transition, humid chambers were prepared and analyzed through optical microscopy. The predictions were performed using MarvinSketch (ChemAxon method), pkCSM, SMARTCyp, and Pred-Skin 3.0 platforms. The chalcones (1E,4E)-1,5-diphenylpenta-1,4-dien-3-one (DB-Acetone), (1E,3E,6E,8E)-1,9-diphenylnona-1,3,6,8-tetraen-5-one (DB-CNM), and (1E,4E)-1,5-bis (4-methoxyphenyl)penta-1,4-dien-3-one (DB-Anisal), as well as fluconazole, completely inhibited fungal morphological transition at the tested concentrations (MC, 1024 <i>μ</i>g/mL, and MC/2, 512 <i>μ</i>g/mL). In silico analyses revealed high intestinal absorption for all chalcones, with DB-Acetone and DB-Anisal showing potential for significant oral bioavailability. Moreover, all compounds demonstrated extensive tissue distribution and the ability to cross the blood-brain barrier. Despite some limitations, DB-Acetone, DB-CNM, and DB-Anisal were predicted to be well tolerated at high doses, with DB-Anisal emerging as the safest candidate based on toxicity profiles. Overall, these findings suggest that the synthetic chalcones studied are promising agents for inhibiting the morphological transition of <i>C. albicans</i> and <i>C. tropicalis</i>, with DB-Anisal showing the most favorable pharmacokinetic and safety profile.</p>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2026 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/cmi/6676802","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147564276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiology and Seasonal Trends of Mycoplasma pneumoniae Infections in Central Inner Mongolia, China (2023–2024): A Population-Based Study","authors":"Wang Tingting,&nbsp;Ren Lifang,&nbsp;Sun Liangliang,&nbsp;Li Xiaocong,&nbsp;Xiao Weili,&nbsp;Cao Jingyuan,&nbsp;Ding Haitao","doi":"10.1155/cmi/1393649","DOIUrl":"https://doi.org/10.1155/cmi/1393649","url":null,"abstract":"&lt;div&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Objective&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;The objective of the study is to investigate the epidemiological characteristics of &lt;i&gt;Mycoplasma pneumoniae&lt;/i&gt; (&lt;i&gt;MP&lt;/i&gt;) infection in the central region of Inner Mongolia from November 2023 to October 2024 and to provide evidence for the effective prevention and control of &lt;i&gt;MP&lt;/i&gt; infections.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Methods&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;A cross-sectional study design was employed, retrospectively including patients from the People′s Hospital of Inner Mongolia Autonomous Region who underwent both &lt;i&gt;MP&lt;/i&gt; nucleic acid (&lt;i&gt;MP&lt;/i&gt;-DNA) testing and &lt;i&gt;MP&lt;/i&gt;-IgM antibody testing during the same period. Chi-square tests were used to compare the positive rates among different sexes, age groups, and seasons. McNemar′s test was applied to evaluate the differences in positive rates between the two methods, and kappa values were used to assess the consistency between the two detection methods. During the study period, 19,330 &lt;i&gt;MP&lt;/i&gt;-DNA tests and 15,335 &lt;i&gt;MP&lt;/i&gt;-IgM tests were conducted. There was no statistically significant difference in the positive rate of &lt;i&gt;MP&lt;/i&gt;-DNA between males and females (28.37% vs. 29.18%, &lt;i&gt;p&lt;/i&gt; &gt; 0.05), but there was a significant difference in the positive rate of &lt;i&gt;MP&lt;/i&gt;-IgM (18.41% vs. 21.07%, &lt;i&gt;p&lt;/i&gt; &lt; 0.001). The highest positive rates were observed in the pediatric group (0–17 years old) for both &lt;i&gt;MP&lt;/i&gt;-DNA (34.77%) and &lt;i&gt;MP&lt;/i&gt;-IgM (24.23%). Overall, the positive rates differed significantly among the age groups (&lt;i&gt;MP&lt;/i&gt;-DNA &lt;i&gt;χ&lt;/i&gt;&lt;sup&gt;2&lt;/sup&gt; = 1215.31, &lt;i&gt;p&lt;/i&gt; &lt; 0.001; &lt;i&gt;MP&lt;/i&gt;-IgM &lt;i&gt;χ&lt;/i&gt;&lt;sup&gt;2&lt;/sup&gt; = 725.42, &lt;i&gt;p&lt;/i&gt; &lt; 0.001). Seasonal analysis revealed that the highest positive rates for both &lt;i&gt;MP&lt;/i&gt;-DNA and &lt;i&gt;MP&lt;/i&gt;-IgM occurred in autumn (August to October) (36.45% vs. 28.23%). The highest positive detection rates for both &lt;i&gt;MP&lt;/i&gt;-DNA and &lt;i&gt;MP&lt;/i&gt;-IgM were found in patients diagnosed with lower respiratory tract infections. Analysis of 3140 subjects who underwent both nucleic acid and antibody testing revealed a statistically significant difference in positive rates between the two methods (&lt;i&gt;p&lt;/i&gt; &lt; 0.001), but the consistency level was low (&lt;i&gt;κ&lt;/i&gt; = 0.26).&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Conclusion&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;The results indicate that &lt;i&gt;MP&lt;/i&gt; infection is prevalent among children and peaks in autumn. The low consistency between the &lt;i&gt;MP&lt;/i&gt;-DNA and &lt;i&gt;MP&lt;/i&gt;-IgM detection methods underscores the importance of combined diagnostic approaches. This study highlights the need for targeted disease surveillance and preventive measures on the basis of demographic and seasonal characteristics in the region.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 ","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2025 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/cmi/1393649","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145905166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of Macrophage Polarization Mediated by Extracellular Vesicle During Bacterial Infection","authors":"Xiaoqin Mou,&nbsp;Li Wang,&nbsp;Jun Wang,&nbsp;Chuang Cheng,&nbsp;Xi Zheng,&nbsp;Jing He,&nbsp;Yueqing Wang,&nbsp;Qingyong Zhang,&nbsp;Lili Zou,&nbsp;Xiaowen Liu","doi":"10.1155/cmi/7976988","DOIUrl":"https://doi.org/10.1155/cmi/7976988","url":null,"abstract":"<p>Extracellular vesicles (EVs) are nanovesicles secreted by cells with lipid bilayer structure, which play a key role in the communication between bacteria and host cells. This paper reviews the role of EVs, particularly bacterial-derived EVs (bEVs) and host cell (e.g., macrophage)-derived exosomes, in the regulation of macrophage polarization during bacterial infection. Macrophages can differentiate into M1 and M2 types with different functions, which is very important in the innate immune response. bEVs and exosomes influence the polarization state of macrophages by carrying a variety of bioactive molecules, such as bacterial effectors, nucleic acids, and lipids, and finally, they are involved in the regulation of host immune and inflammatory responses. In addition, EVs show great potential in early disease diagnosis, vaccine development, and targeted therapy. However, the application of EVs still faces many challenges, including the development of isolation and purification techniques, the establishment of standardized processes, and the clarity of specific mechanisms. In this review, the mechanism of bEVs and exosomes in regulating macrophage polarization is systematically summarized, their potential applications in infectious diseases are explored, and directions for future research are proposed. With a deeper understanding of the function and regulatory mechanisms, EVs are expected to become a new diagnostic marker and a therapeutic tool, providing new strategies for the prevention and treatment of infectious diseases.</p>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2025 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/cmi/7976988","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145626364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Fluorescently Tagged Fusarium oxysporum f. sp. lini Strain Using Advanced Genetic Labeling and Its Pathogenicity Assessment","authors":"Anna Domańska,&nbsp;Kamil Kostyn,&nbsp;Marta Burgberger,&nbsp;Anna Kulma,&nbsp;Wioleta Wojtasik,&nbsp;Aleksandra Boba","doi":"10.1155/cmi/9586770","DOIUrl":"https://doi.org/10.1155/cmi/9586770","url":null,"abstract":"<p>Research on <i>Fusarium oxysporum</i> f. sp. <i>lini</i> (Foln), a fungal pathogen of <i>Linum usitatissimum</i> L. (flax) responsible for significant crop losses, requires effective methods for visualizing fungus–plant interactions to improve understanding of this pathosystem. In this study, transgenic Foln strains expressing GFP or dsRed fluorescent markers were generated via <i>Agrobacterium</i>-mediated transformation. Several <i>Agrobacterium tumefaciens</i> strains were tested, and those with the highest transformation efficiency were selected. Transformants were screened based on phenotypic characteristics and further validated using molecular biology techniques. Since transgenesis can influence a fungus′s ability to infect its host, the pathogenicity of the generated Foln lines was assessed by measuring the expression levels of pathogenesis-related genes and ROS metabolism-related genes, alongside quantifying O₂⁻ and H₂O₂ levels in the infected plant tissue. The presence of dsRed and GFP fluorescent markers was confirmed using fluorescence microscopy. Ultimately, two transformants exhibiting pathogenic characteristics similar to the wild-type Foln strain were selected. These transformants represent valuable tools for further studies of Foln pathogenesis in flax. Additionally, the workflow developed here can be adapted to generate fluorescent transformants of other <i>F. oxysporum</i> pathogenic strains.</p>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2025 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/cmi/9586770","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145469768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to “miR-193b Represses Influenza A Virus Infection by Inhibiting Wnt/β-Catenin Signalling”","authors":"","doi":"10.1155/cmi/9809676","DOIUrl":"https://doi.org/10.1155/cmi/9809676","url":null,"abstract":"<p>X. Yang, C. Zhao, G. Bamunuarachchi, et al., “miR-193b Represses Influenza A Virus Infection by Inhibiting Wnt/<i>β</i>-Catenin Signalling,” <i>Cellular Microbiology</i> 21, no. 5 (2019): e13001, https://doi.org/10.1111/cmi.13001.</p><p>In the article titled “miR-193b Represses Influenza A Virus Infection by Inhibiting Wnt/<i>β</i>-Catenin Signalling,” there was an error in Figure 2a, where the images duplicated in miR-Con and miR-1-1 as well as miR-193b and miR-509-1 blots. The corrected figure is shown below and is listed as Figure 1:</p><p>We apologize for this error.</p>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2025 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/cmi/9809676","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145317188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic Changes in Oral Fibroblasts After Exposure to Bacterial Extracellular Vesicles From Three Strains of Porphyromonas gingivalis","authors":"Helene Rygvold Haugsten,&nbsp;Aleksandra Lipka,&nbsp;Anne Karin Kristoffersen,&nbsp;Morten Enersen,&nbsp;Anni Nieminen,&nbsp;Tine M. Søland,&nbsp;Trude M. Haug,&nbsp;Hilde Kanli Galtung","doi":"10.1155/cmi/7515004","DOIUrl":"https://doi.org/10.1155/cmi/7515004","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p><i>Porphyromonas gingivalis</i> is a keystone pathogen in periodontitis that releases bacterial extracellular vesicles (bEVs) with a content derived from the membrane and cytosol of the bacteria itself. The bEVs are taken up by host tissue cells, such as oral fibroblasts, yet their effects on human cells remain incompletely understood.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The aim of this study was to investigate the impact of bEVs on human oral fibroblasts. Targeted liquid chromatography–mass spectrometry was used to assess changes in the metabolome of these cells following exposure to bEVs from three strains of <i>P. gingivalis</i> (ATCC 33277, A7A1-28, and W83). Metabolite content of the bEVs from each strain was also characterized.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Result</h3>\u0000 \u0000 <p>Metabolomic analyses revealed both common and strain-specific metabolites in the bEVs from these strains. Additionally, pathways mainly associated with amino acid metabolism were enriched for all. Following exposure to bEVs, the fibroblasts exhibited a metabolic shift in energy metabolism. In particular, fibroblasts incubated with bEVs from ATCC 33277 had a unique profile of enriched pathways after 10 min. However, after 24 h, this profile changed and became more similar to the profile in fibroblasts incubated with bEVs from the two more pathogenic strains. Pathway analysis revealed enrichment of <i>glycerophospholipid</i> and <i>butanoate metabolism</i> for fibroblasts exposed to all the bEVs. Additionally, altered sphingolipid metabolism was observed in fibroblasts following exposure to bEVs from ATCC 33277.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Our findings demonstrate that bEVs derived from <i>P. gingivalis</i> strains can induce changes in cellular processes in fibroblasts. This suggests a role of bEVs in the pathogenesis of periodontitis.</p>\u0000 </section>\u0000 </div>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2025 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/cmi/7515004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145272795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the Anticancer Effect of Streptomyces sp. M12 Extract Against Glioblastoma U87_MG Cell Line","authors":"Hasti Rezaee,&nbsp;Ensieh Salehghamari,&nbsp;Atefeh Khamoushi,&nbsp;Hanieh Jalali,&nbsp;Mahsa Asadi Ardalani","doi":"10.1155/cmi/7344471","DOIUrl":"https://doi.org/10.1155/cmi/7344471","url":null,"abstract":"<p>Glioblastoma, the most aggressive and fatal form of brain tumor, is characterized by rapid growth, extensive invasion of surrounding tissues, and significant angiogenesis. These and other types of cancer remain a leading cause of mortality worldwide, with conventional treatments such as chemotherapy, radiation, and surgery often limited by significant side effects. This has led to the pursuit of novel therapeutic approaches, including bacterial therapy, which utilizes bacteria’s unique ability to target tumor microenvironments and deliver therapeutic agents. This research examines the antitumor effects of <i>Streptomyces</i> sp. M12 extract on the U87_MG glioblastoma cell line. The cytotoxicity of the extract was assessed using the MTT assay. Additionally, flow cytometry and scratch assays were conducted to evaluate cell migration. Furthermore, gene expression analysis for <i>Bax</i>, <i>Bcl-2</i>, <i>Caspase-8</i>, and <i>Caspase-9</i> was performed to determine apoptotic effects. The MTT assay indicated strong anticancer activity with an IC₅₀ value of 17.72 after 96 h of treatment. In vitro studies showed that the extract significantly promotes apoptosis, as evidenced by an 83.6% increase in apoptosis rates via flow cytometry. Moreover, the scratch assay demonstrated that the extract inhibited U87_MG cell migration, indicating antimetastatic potential. Real-time PCR analysis results revealed a 1.972-fold increase in <i>Caspase-8</i> expression and a 0.468-fold decrease in <i>Caspase-9</i> expression (<i>p</i> ≤ 0.001), suggesting that apoptosis is triggered through the extrinsic pathway. These findings emphasize the dual cytotoxic and antimigratory effects of the strain M12 extract, making it a promising candidate for glioblastoma treatment. Further investigation is necessary to clarify the molecular mechanisms involved and confirm its therapeutic potential in preclinical and clinical settings. This study highlights the significance of natural products, particularly those derived from <i>Streptomyces</i> sp. M12, an innovative cancer treatment strategy.</p>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2025 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/cmi/7344471","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Critical Roles of Klebsiella pneumoniae Enterobactin in NLRP3 Inflammasome Activation in Airway Epithelial Cells","authors":"Julien Verlaguet,&nbsp;Damien Balestrino,&nbsp;Laurence Ollivier-Nakusi,&nbsp;Christiane Forestier,&nbsp;Marjolaine Vareille-Delarbre","doi":"10.1155/cmi/6397050","DOIUrl":"https://doi.org/10.1155/cmi/6397050","url":null,"abstract":"<p><i>Klebsiella pneumoniae</i> is a ubiquitous Gram-negative bacterium and a common cause of pneumonia, which leads to intense lung injury and mortality that are correlated with deregulated inflammation. Emerging evidence indicates that the NLRP3 inflammasome plays a critical part in regulating inflammatory processes in various infectious diseases. However, its role in <i>K. pneumoniae</i> infections remains elusive. In this study, we identified a siderophore, enterobactin (Ent), from <i>K. pneumoniae</i> as a key factor that induces NLRP3 activation in both the pulmonary epithelial cell line A549 and lung tissue from <i>K. pneumoniae</i>–infected mice. A549 epithelial cells infected with an Ent-deficient mutant (<i>ΔentB</i>) had lower <i>Nlrp3</i>, <i>Asc</i>, and <i>Pro-caspase-1</i> gene expression, caspase-1 activity, and IL-18 secretion than cells infected with wild-type <i>K. pneumoniae</i>. No such effect was observed with THP-1 macrophages. Ent induced NLRP3 activation and IL-18 production in lung tissue of mice intranasally infected by <i>K. pneumoniae</i> strains. Interestingly, the recruitment of immune cells and production of inflammatory cytokines and chemokines were comparable in wild-type and <i>ΔentB</i> strain<i>–</i>infected mice. Taken together, our findings provide the first example of Ent playing a role in host inflammation control by targeting NLRP3.</p>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2025 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/cmi/6397050","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144918857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}