Cellular Microbiology最新文献

{"title":"Investigating the Anticancer Effect of Streptomyces sp. M12 Extract Against Glioblastoma U87_MG Cell Line","authors":"Hasti Rezaee,&nbsp;Ensieh Salehghamari,&nbsp;Atefeh Khamoushi,&nbsp;Hanieh Jalali,&nbsp;Mahsa Asadi Ardalani","doi":"10.1155/cmi/7344471","DOIUrl":"https://doi.org/10.1155/cmi/7344471","url":null,"abstract":"<p>Glioblastoma, the most aggressive and fatal form of brain tumor, is characterized by rapid growth, extensive invasion of surrounding tissues, and significant angiogenesis. These and other types of cancer remain a leading cause of mortality worldwide, with conventional treatments such as chemotherapy, radiation, and surgery often limited by significant side effects. This has led to the pursuit of novel therapeutic approaches, including bacterial therapy, which utilizes bacteria’s unique ability to target tumor microenvironments and deliver therapeutic agents. This research examines the antitumor effects of <i>Streptomyces</i> sp. M12 extract on the U87_MG glioblastoma cell line. The cytotoxicity of the extract was assessed using the MTT assay. Additionally, flow cytometry and scratch assays were conducted to evaluate cell migration. Furthermore, gene expression analysis for <i>Bax</i>, <i>Bcl-2</i>, <i>Caspase-8</i>, and <i>Caspase-9</i> was performed to determine apoptotic effects. The MTT assay indicated strong anticancer activity with an IC₅₀ value of 17.72 after 96 h of treatment. In vitro studies showed that the extract significantly promotes apoptosis, as evidenced by an 83.6% increase in apoptosis rates via flow cytometry. Moreover, the scratch assay demonstrated that the extract inhibited U87_MG cell migration, indicating antimetastatic potential. Real-time PCR analysis results revealed a 1.972-fold increase in <i>Caspase-8</i> expression and a 0.468-fold decrease in <i>Caspase-9</i> expression (<i>p</i> ≤ 0.001), suggesting that apoptosis is triggered through the extrinsic pathway. These findings emphasize the dual cytotoxic and antimigratory effects of the strain M12 extract, making it a promising candidate for glioblastoma treatment. Further investigation is necessary to clarify the molecular mechanisms involved and confirm its therapeutic potential in preclinical and clinical settings. This study highlights the significance of natural products, particularly those derived from <i>Streptomyces</i> sp. M12, an innovative cancer treatment strategy.</p>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2025 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/cmi/7344471","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Critical Roles of Klebsiella pneumoniae Enterobactin in NLRP3 Inflammasome Activation in Airway Epithelial Cells","authors":"Julien Verlaguet,&nbsp;Damien Balestrino,&nbsp;Laurence Ollivier-Nakusi,&nbsp;Christiane Forestier,&nbsp;Marjolaine Vareille-Delarbre","doi":"10.1155/cmi/6397050","DOIUrl":"https://doi.org/10.1155/cmi/6397050","url":null,"abstract":"<p><i>Klebsiella pneumoniae</i> is a ubiquitous Gram-negative bacterium and a common cause of pneumonia, which leads to intense lung injury and mortality that are correlated with deregulated inflammation. Emerging evidence indicates that the NLRP3 inflammasome plays a critical part in regulating inflammatory processes in various infectious diseases. However, its role in <i>K. pneumoniae</i> infections remains elusive. In this study, we identified a siderophore, enterobactin (Ent), from <i>K. pneumoniae</i> as a key factor that induces NLRP3 activation in both the pulmonary epithelial cell line A549 and lung tissue from <i>K. pneumoniae</i>–infected mice. A549 epithelial cells infected with an Ent-deficient mutant (<i>ΔentB</i>) had lower <i>Nlrp3</i>, <i>Asc</i>, and <i>Pro-caspase-1</i> gene expression, caspase-1 activity, and IL-18 secretion than cells infected with wild-type <i>K. pneumoniae</i>. No such effect was observed with THP-1 macrophages. Ent induced NLRP3 activation and IL-18 production in lung tissue of mice intranasally infected by <i>K. pneumoniae</i> strains. Interestingly, the recruitment of immune cells and production of inflammatory cytokines and chemokines were comparable in wild-type and <i>ΔentB</i> strain<i>–</i>infected mice. Taken together, our findings provide the first example of Ent playing a role in host inflammation control by targeting NLRP3.</p>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2025 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/cmi/6397050","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144918857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut Microbiota–Derived Metabolites as Potential Therapeutics in Mitochondrial Dysfunction-Driven Intestinal Inflammatory Diseases","authors":"Gayatri Reghu,&nbsp;Pardhipa Nandigam,&nbsp;Ashna Fathima,&nbsp;Trinath Jamma","doi":"10.1155/cmi/9644892","DOIUrl":"https://doi.org/10.1155/cmi/9644892","url":null,"abstract":"<p>Inflammatory bowel diseases are a group of chronic diseases with increasing global incidence and rising morbidity and mortality, contributing to a significant health burden. Mainstream treatments for IBD are focused on controlling inflammation and alleviating the symptoms, also aiming for intestinal barrier repair. Understanding the cellular and molecular mechanisms underlying intestinal inflammatory disorders is the key to developing more targeted therapies to treat IBD. The growth, differentiation, and proliferation of intestinal epithelial cells require energy. Since mitochondria provide the energy also required for intestinal epithelial cells to maintain homeostasis and barrier integrity, their dysfunction can lead to cell death and inflammation. While several studies have confirmed the link between gut microbial metabolites and mitochondrial function, conflicting results from studies in IBD models have presented challenges to a detailed understanding of this area. This review focuses on presenting data from existing literature and highlighting the therapeutic potential of gut microbiota–derived metabolites in restoring mitochondrial function, which may likely develop into therapeutic interventions in IBD.</p><p><b>Trial Registration:</b> ClinicalTrials.gov identifier: NCT01473524, NCT03724175</p>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2025 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/cmi/9644892","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Silico Study of Bis(6-Amino-1,3-Dimethylpyrimidine-2,4(1H,3H)-Diones) as EGFR Inhibitors: Synthesis, DFT, Molecular Docking, and MD Simulation Studies","authors":"Zeynab masoumi,&nbsp;Mohammad Bayat,&nbsp;Davood Gheidari","doi":"10.1155/cmi/5585552","DOIUrl":"https://doi.org/10.1155/cmi/5585552","url":null,"abstract":"<p>The epidermal growth factor receptor (EGFR), a protein located on the cell surface, belongs to the tyrosine kinase family and plays a crucial role in cell development and proliferation. Abnormal expression or mutations in the EGFR gene can lead to non–small cell lung cancer. Although established EGFR inhibitors have been effective in the treatment of cancer, they are associated with several side effects. As a result, there is an urgent need to develop novel EGFR inhibitors that can effectively target the receptor while causing no adverse side effects. In this study, a series of bis(6-amino-1,3-dimethylpyrimidine-2,4(1<i>H</i>,3<i>H</i>)-dione derivatives was synthesized in water at room temperature without the use of any catalyst, and pharmaceutical properties are investigated. Computational methods, including density functional theory (DFT), molecular docking, and molecular dynamics (MD), were utilized to investigate the chemical properties, drug-like characteristics, and anticancer potential of the molecule. Quantum chemical calculations indicated that the molecules are relatively stable and exhibit significant electrophilic properties. The analysis of HOMO-LUMO contour maps was conducted to illustrate charge density distributions that may be associated with biological activity. Docking studies with EGFR enzymes indicated that all compounds demonstrated favorable binding affinities, with docking scores ranging from −4.412 to −6.158 kcal/mol. Particularly, Compound 3f, with an energy of −6.158 kcal/mol, showed the best binding affinity, outperforming the native ligand, which had a docking score of −5.076 kcal/mol. The stability of the EGFR-3f complex is significantly enhanced by the formation of five conventional hydrogen bonds and one carbon–hydrogen bond in ligand–protein interactions. MD simulations, which included analyses such as root mean square deviation (RMSD), root mean square fluctuation (RMSF), radius of gyration (rGyr), molecular surface area (MolSA), and polar surface area (PSA), were conducted on the EGFR-3f complex. It was found that the EGFR-3f complex is stable, and the results show that Compound 3f has a strong interaction with the target enzyme.</p>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2025 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/cmi/5585552","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144688083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cunning Pathogen Tactics: Neutrophil Movement Influenced by Bacterial Attractants in the Experimental Model","authors":"Svetlana N. Pleskova,&nbsp;Nikolay А. Bezrukov,&nbsp;Ekaterina N. Gorshkova,&nbsp;Eseniya V. Otstavnova,&nbsp;Dmitry V. Novikov","doi":"10.1155/cmi/8859162","DOIUrl":"https://doi.org/10.1155/cmi/8859162","url":null,"abstract":"<p>To explore the peculiarities of neutrophil motility, two models of chemoattraction were created: a horizontal model, where a container with bacterial chemoattractant was attached laterally to the endotheliocyte monolayer, and a vertical model, simulating a pyemic focus in the lower part of the modified Boyden chamber. Low-molecular weight product secretion and/or degradation of <i>Enterococcus faecalis</i> caused “disorientations” of neutrophil migration with hyperproduction reactive oxygen species (ROS) by immune cells, while <i>Proteus mirabilis</i> inhibited both migration of most neutrophils and the production of ROS by them, while the activity of the remaining uninhibited neutrophils increased. Neutrophils generated ROS during migration, especially actively in the case of a large number of mobile cells (under stimulation with low-molecular weight product secretion and/or degradation of <i>Enterococcus faecalis</i> and <i>Escherichia coli</i>). Using high-resolution microscopy, it was shown that low-activity neutrophils cause changes in the morphology of endothelial cells during migration more than high-activity neutrophils. In the vertical migration model, the morphology of endothelial cells significantly changed during neutrophils diapedesis. It was observed that space between endothelial cells was increased (especially in the case of neutrophil swarming).</p>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2025 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/cmi/8859162","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144573583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Malaria Cytoskeletal Proteins Require Alveolin–Alveolin Interactions for Differential Localization","authors":"Ana Karla Cepeda Diaz,&nbsp;Peter S. Back,&nbsp;Sreelakshmi K. Sreenivasamurthy,&nbsp;Jeffrey D. Dvorin","doi":"10.1155/cmi/4530231","DOIUrl":"https://doi.org/10.1155/cmi/4530231","url":null,"abstract":"<p>The alveolins are a family of intermediate filament-like proteins that form cytoskeletal structures in both free-living and parasitic members of the alveolate kingdom. Despite their important functions, the alveolins’ biochemical properties and organizing principles are still poorly understood. Here, we characterize four alveolins of <i>Plasmodium falciparum</i>, the deadliest malaria parasite, to understand how alveolin domains mediate protein–protein interactions and highly specific recruitment to substructures of the cytoskeleton. Unexpectedly, we uncover variable dependence on alveolin domains for each substructure rather than an overarching mechanism. While <i>Pf</i>IMC1e requires 1f to be sequentially recruited to the basal complex, <i>Pf</i>IMC1c and <i>Pf</i>IMC1g do not require interactions with each other to localize properly to the inner membrane complex. Moreover, alveolin domains are not interchangeable—they contain unique signatures for specialized localization. Finally, we identify a region outside the alveolin domain of <i>Pf</i>IMC1e that is important for basal complex recruitment. These results provide direct evidence that alveolin domains mediate both alveolin–alveolin interactions and compartment-specific localization.</p>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2025 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/cmi/4530231","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144536973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pseudogene BNIP3P1 Regulates H. pylori–Induced Apoptosis in Gastric Mucosal by Acting on the miR-411-5p/BNIP3 Axis","authors":"Andong Zhang,&nbsp;Xin Yan,&nbsp;Ningzhe Li,&nbsp;Shiying Yan,&nbsp;Shu Li,&nbsp;Hua Yang,&nbsp;Yimeng Liu,&nbsp;Jian Zhang,&nbsp;Jia Wang,&nbsp;Jian Zhao,&nbsp;Mei Cao","doi":"10.1155/cmi/6763794","DOIUrl":"https://doi.org/10.1155/cmi/6763794","url":null,"abstract":"<p><i>Helicobacter pylori</i> (<i>H. pylori</i>) is one of the major causes of gastric mucosal damage, and infection with <i>H. pylori</i> induces an immune response with gastric mucosal cells, which reduces gastric mucosal damage. The pseudogene <i>BNIP3P1</i>, sharing a remarkable 95.92% similarity with its well-characterized counterpart <i>BNIP3</i>, has largely remained unexplored. To elucidate the role of <i>BNIP3P1</i> in gastric mucosal damage induced by <i>H. pylori</i> infection, we meticulously constructed both in vivo and in vitro models. Gene chip sequencing, dual-luciferase assays, and cellular phenotyping were detected. We uncovered a compelling positive correlation between the duration of <i>H. pylori</i> infection and <i>BNIP3</i> overexpression at both the mRNA and protein levels. Intriguingly, overexpression of BNIP3 was found to effectively impede the proliferation and migration of human gastric epithelial cells (GES-1). Furthermore, we identified <i>miR-411-5p</i> as a direct regulator of <i>BNIP3</i>, targeting its 3 ^′UTR region and suppressing its expression during <i>H. pylori</i> infection. Notably, <i>BNIP3P1-</i> 3^′UTR was observed to competitively bind <i>miR-411-5p</i>, leading to the upregulation of <i>BNIP3</i> expression. Furthermore, overexpression of <i>BNIP3P1</i> was associated with a marked decrease in GES-1 cell proliferation and a concomitant acceleration of apoptosis. Our findings suggest that <i>BNIP3P1</i> functions as a competing endogenous RNA (ceRNA) within the <i>BNIP3/miR-411-5p</i> axis during <i>H. pylori</i> infection, which ultimately hinders cell proliferation and promotes apoptosis in GES-1 cells. This study sheds light on the intricate mechanisms underlying <i>H. pylori</i> infection of GES-1 cells.</p>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2025 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/cmi/6763794","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143925824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diverse Effects of Fluoro-Phenyl-Styrene-Sulfonamide (FPSS) on Transcription Factor Sigma B Regulons in Gram-Positive Bacillales","authors":"Sirirak Supa-amornkul,&nbsp;Ramita Chalearmchutidath,&nbsp;Nichaporn Nattawut,&nbsp;Phimkanya Liu,&nbsp;Soraya Chaturongakul","doi":"10.1155/cmi/2175797","DOIUrl":"https://doi.org/10.1155/cmi/2175797","url":null,"abstract":"<p><i>Bacillales</i> is an order of Gram-positive bacteria comprising well-known genera such as <i>Staphylococcus</i>, <i>Listeria</i>, and <i>Bacillus. Staphylococcus aureus</i> has been shown to cause various types of tissue and systemic infections in humans and livestock. Some isolates can be highly resistant to antibiotics hence the need to identify better drugs and drug targets. Previous studies have shown that (E)-N-(4-fluorophenyl)-2-phenylethene sulfonamide (FPSS) could inhibit activities of a bacteria-specific transcription factor sigma B. Sigma B regulons in <i>Bacillus subtilis</i> and in <i>Listeria monocytogenes</i>, including virulence factor genes in <i>L. monocytogenes</i>, were decreased. Since sigma B is also presented in <i>S. aureus</i>, we initially postulated the use of FPSS to target <i>S. aureus</i> sigma B activity and to attenuate its virulence gene expression. Surprisingly, qRT-PCR results revealed that FPSS induced the expression of sigma B-dependent gene <i>asp23</i>. RNAseq results showed 39 <i>S. aureus</i> genes were affected by FPSS (37 genes were upregulated and two genes were downregulated). FPSS had no effect on expression of <i>sigB</i> operon in <i>S. aureus</i>. Therefore, we hypothesized that the effects of FPSS on sigma B regulons in <i>L. monocytogenes</i>, <i>B. subtilis</i>, and <i>S. aureus</i> were different. FPSS may target the expression of upstream regulators of sigma B (Rsbs), particularly the stressosome proteins which are lacking in <i>S. aureus.</i> Indeed, in <i>L. monocytogenes</i>, FPSS significantly increased the <i>rsbR</i> expression and could, thereby, dampen the sigma B activity and downregulate the expression of sigma B-dependent virulence genes in <i>L. monocytogenes</i>. This study proposes a narrower spectrum of FPSS application to listerial infections, and not staphylococcal infections.</p>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2025 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/cmi/2175797","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143909385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Chicken Gut Metagenome at Different Egg-Laying Stages and Its Correlation With Egg Production Performance","authors":"Ziyu Li,&nbsp;Bo Zeng,&nbsp;Tao Wang,&nbsp;Lizhi Lu,&nbsp;Hang Zhong,&nbsp;Yuan Su,&nbsp;Diyan Li,&nbsp;Wenwu Xu","doi":"10.1155/cmi/5586545","DOIUrl":"https://doi.org/10.1155/cmi/5586545","url":null,"abstract":"<p>To investigate the investigation of gut microbial communities to chicken egg production performance during different laying stages, we conducted a comparative study of the diversity, composition, and function of microbial communities in the fecal and cecal contents of six hens at the early stage, 25 at the peak stage, and 15 at the late stage. We obtained clean data averaging 13.40 and 13.79 Gb for each of the fecal and cecal content samples. The metagenomic analysis revealed significant differences in fecal and cecal content microbial diversity during different laying stages, especially during the peak stage, and the microbiota structure of fecal contents was more stable compared to that of cecal contents. The dominant microflora in the fecal contents during the peak stage were Firmicutes (74.84%) and <i>Lactobacillus</i> (28.13%). The dominant microflora of cecal contents during different stages were basically the same at the phylum level and genus level. During the peak stage, the dominant bacteria shared by microorganisms in the fecal and cecal contents were <i>Lactobacillus</i>. Functional analyses of the gut microbiome indicate that fecal and cecal content microbes have different functional capacities at different egg-laying stages. We therefore hypothesized that the gut microbiome would vary with laying stage and have a non-negligible effect on egg production performance. These results improved our ability to provide some theoretical basis for feeding management of laying hens during different laying stages and provided insights into the influence of the gut microbiota on the laying performance of chickens.</p>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2025 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/cmi/5586545","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variations in Lorica Morphology and Element Composition in the Euglenoid Trachelomonas hispida var. coronata Under the Influence of Fe and Mn Supply","authors":"Małgorzata Poniewozik,&nbsp;Mateusz Kudlak,&nbsp;Emil Zięba","doi":"10.1155/cmi/6716112","DOIUrl":"https://doi.org/10.1155/cmi/6716112","url":null,"abstract":"<p><i>Trachelomonas hispida</i> var. <i>coronata</i> is one of the most widely reported varieties of <i>T</i>. <i>hispida</i> from water bodies worldwide. The specimens of this variety, apart from their species-specific features, such as an ellipsoidal lorica covered with strong, sharp spines, have a crown consisting of long spines surrounding the apical pore opening. The process of lorica formation is poorly understood, and in the few studies dealing with this topic, results indicate that these taxa and the entire species can produce lorica completely devoid of spines, a diacritic feature of the species. In our study, we observed in detail the formation process of the lorica in this taxon under different chemical conditions in relation to the concentration of the basic elements, Fe and Mn, which are saturated in trachelomonad lorica. The results showed that in the Fe-enriched medium, monads formed delicate, porous, spiny envelopes, whereas in the Mn-enriched medium, the loricae were more solid and less porous and had weaker developed spines; rather, they were in the form of short papillae. Differences were also observed in the structure surrounding the apical pore, which was developed differently in the two sets of media modifications (Fe- or Mn-supplemented media). We also observed different elemental compositions and colouration of loricae of cells growing in different media. This revealed that the features considered during the process of species identification are very unstable making the entire exercise highly complicated. Our research also shows that a broad discussion of the problem should be undertaken, and modern methods must be developed to unravel the complexities not only within the species but also within the entire <i>Trachelomonas</i>.</p>","PeriodicalId":9844,"journal":{"name":"Cellular Microbiology","volume":"2025 1","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/cmi/6716112","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143840695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}