gpi锚定蛋白编码基因Aog185的缺失通过破坏跨膜运输稳态来阻碍少孢子节虫的生长和捕获线虫的效率

IF 2.6 2区 生物学 Q3 CELL BIOLOGY
Hui Peng, Hengqian Lu, Xinyuan Dong, Xiao Liang, Kangliang Sheng, Jingmin Wang, X. Kong, X. Zha, Yongzhong Wang
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引用次数: 0

摘要

诱捕线虫真菌(Nematode-trapping fungus, NTF)是一种重要的线虫捕食者,它可以通过形成特定的诱捕装置捕获线虫。然而,对附着在菌丝或诱捕细胞表面的细胞表面蛋白的作用和机制了解有限。本文研究了推定的gpi锚定蛋白编码基因Aog185对寡孢单胞菌生长和捕获线虫效率的影响。与野生型(WT)菌株相比,ΔAog185突变体生长更慢,分生孢子减少20%,分生孢子萌发延迟,产生的陷阱更少,捕获线虫的效率降低,对化学胁迫更敏感。转录组学分析表明,WT和ΔAog185突变株之间存在大量跨膜运输相关基因的差异表达。Aog185缺失会破坏细胞膜和细胞骨架的固有成分。具体来说,敲除Aog185破坏了吞噬、细胞自噬和氧化磷酸化过程中的跨膜运输稳态,这些过程与细胞和细胞器膜的融合、离子和底物的运输以及能量代谢有关。因此,假定的gpi锚定蛋白编码基因Aog185可能有助于NTF和线虫捕获的生活方式转换,并且Aog185基因对细胞跨膜运输的影响被认为是这一过程的关键。本研究为研究Aog185基因在NTF诱捕线虫过程中的作用机制提供了新的思路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Deletion of a Putative GPI-Anchored Protein-Encoding Gene Aog185 Impedes the Growth and Nematode-Trapping Efficiency of Arthrobotrys oligospora by Disrupting Transmembrane Transport Homeostasis
Nematode-trapping fungus (NTF) is a crucial predator of nematodes, which can capture nematodes by developing specific trapping devices. However, there is limited understanding of the role and mechanism of cell surface proteins attached to the surface of mycelia or trapping cells. Here, the effects of a putative GPI-anchored protein-encoding gene Aog185 on the growth and nematode-trapping efficiency of A. oligospora were investigated. Compared to the wild-type (WT) strain, the ΔAog185 mutant grew more slowly, exhibited a 20% decrease in conidiation, delayed conidial germination, generated fewer traps, attenuated nematode trapping efficiency, and was more sensitive to chemical stressors. Transcriptomic analysis indicated that a large number of transmembrane transport-related genes were differentially expressed between the WT and ΔAog185 mutant strains. Aog185 deletion could damage the intrinsic components of the membrane and cytoskeleton. Specifically, knockout of Aog185 disrupted transmembrane transport homeostasis during the phagocytosis, cell autophagy, and oxidative phosphorylation processes, which were associated with the fusion of cells and organelle membranes, transport of ions and substrates, and energy metabolism. Hence, the putative GPI-anchored protein-encoding gene Aog185 may contribute to the lifestyle switch of NTF and nematode capture, and the effect of Aog185 gene on cell transmembrane transport is considered key to this process. Our findings provide new insights into the mechanism of Aog185 gene during the process of nematode trapping by NTF.
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来源期刊
Cellular Microbiology
Cellular Microbiology 生物-微生物学
CiteScore
9.70
自引率
0.00%
发文量
26
审稿时长
3 months
期刊介绍: Cellular Microbiology aims to publish outstanding contributions to the understanding of interactions between microbes, prokaryotes and eukaryotes, and their host in the context of pathogenic or mutualistic relationships, including co-infections and microbiota. We welcome studies on single cells, animals and plants, and encourage the use of model hosts and organoid cultures. Submission on cell and molecular biological aspects of microbes, such as their intracellular organization or the establishment and maintenance of their architecture in relation to virulence and pathogenicity are also encouraged. Contributions must provide mechanistic insights supported by quantitative data obtained through imaging, cellular, biochemical, structural or genetic approaches.
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