Application of Bioinformatics Tools for the Prediction of Helper MicroRNAs for Improvement of Oncolytic Virus Efficacy

IF 2.6 2区 生物学 Q3 CELL BIOLOGY
Nasrin Rastegarvand, H. Soleimanjahi, E. Arefian, M. Pourkarim
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引用次数: 0

Abstract

Purpose. Oncolytic Reoviruses, as a self-limiting virus, can be used in cancer treatment, because they have the ability to replicate in tumor cells selectively and destroy them. Studies show that some immune response proteins may interfere with the virus life cycle. So, the main aim of this bioinformatic study is to check which microRNA is able to target some reovirus inhibitory proteins. Experimental Design. By use of online bioinformatics software, the microRNAs that could target inhibitory genes were selected. Then, other features like content ++ score and cell type were checked and finally the eligible microRNAs were determined. Results. After choosing 15 inhibitory proteins, analysis was performed and finally 37 microRNAs which could target inhibitory proteins in colorectal cell lines were selected. In the end, by investigation of web-based tools, just two microRNAs were finalized. Conclusions and Clinical Relevance. This bioinformatic study shows that microRNA-140 and microRNA-92a have the potential to target some inhibitory proteins which interfere with oncolytic Reovirus replication and it may help in the optimal use of this virus as a cancer treatment. Because selective reproduction of Reovirus in tumor cells, as a nonchemical therapy, can be a good way to overcome this disease with broad advantages.
应用生物信息学工具预测辅助microrna以提高溶瘤病毒的疗效
意图溶癌呼肠孤病毒作为一种自限性病毒,可以用于癌症治疗,因为它们具有选择性地在肿瘤细胞中复制并破坏它们的能力。研究表明,一些免疫反应蛋白可能会干扰病毒的生命周期。因此,这项生物信息学研究的主要目的是检查哪种微小RNA能够靶向某些呼肠孤病毒抑制蛋白。实验设计。利用在线生物信息学软件,筛选出能靶向抑制基因的微小RNA。然后,检查其他特征,如内容++评分和细胞类型,最终确定合格的微小RNA。后果在筛选出15种抑制蛋白后,进行分析,最终筛选出37种可靶向结直肠癌细胞系中抑制蛋白的微小RNA。最后,通过对基于网络的工具的调查,只有两个微小RNA最终确定。结论和临床相关性。这项生物信息学研究表明,微小RNA-140和微小RNA-92a有可能靶向一些干扰溶瘤性呼肠弧病毒复制的抑制蛋白,它可能有助于将这种病毒作为癌症治疗的最佳用途。因为在肿瘤细胞中选择性繁殖呼肠孤病毒,作为一种非化学疗法,是克服这种疾病的好方法,具有广泛的优势。
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来源期刊
Cellular Microbiology
Cellular Microbiology 生物-微生物学
CiteScore
9.70
自引率
0.00%
发文量
26
审稿时长
3 months
期刊介绍: Cellular Microbiology aims to publish outstanding contributions to the understanding of interactions between microbes, prokaryotes and eukaryotes, and their host in the context of pathogenic or mutualistic relationships, including co-infections and microbiota. We welcome studies on single cells, animals and plants, and encourage the use of model hosts and organoid cultures. Submission on cell and molecular biological aspects of microbes, such as their intracellular organization or the establishment and maintenance of their architecture in relation to virulence and pathogenicity are also encouraged. Contributions must provide mechanistic insights supported by quantitative data obtained through imaging, cellular, biochemical, structural or genetic approaches.
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