Cell Regeneration最新文献_第8页

Correction: Cilia regeneration requires an RNA splicing factor from the ciliary base. 更正:纤毛再生需要来自纤毛基部的RNA剪接因子。

Cell Regeneration Pub Date : 2022-11-09 DOI: 10.1186/s13619-022-00147-2

Kaiming Xu, Guangshuo Ou

引用次数: 0

Mechanical stretching boosts expansion and regeneration of intestinal organoids through fueling stem cell self-renewal. 机械拉伸通过促进干细胞自我更新来促进肠道类器官的扩张和再生。

Cell Regeneration Pub Date : 2022-11-02 DOI: 10.1186/s13619-022-00137-4

Fanlu Meng, Congcong Shen, Li Yang, Chao Ni, Jianyong Huang, Kaijun Lin, Zanxia Cao, Shicai Xu, Wanling Cui, Xiaoxin Wang, Bailing Zhou, Chunyang Xiong, Jihua Wang, Bing Zhao

{"title":"Mechanical stretching boosts expansion and regeneration of intestinal organoids through fueling stem cell self-renewal.","authors":"Fanlu Meng, Congcong Shen, Li Yang, Chao Ni, Jianyong Huang, Kaijun Lin, Zanxia Cao, Shicai Xu, Wanling Cui, Xiaoxin Wang, Bailing Zhou, Chunyang Xiong, Jihua Wang, Bing Zhao","doi":"10.1186/s13619-022-00137-4","DOIUrl":"https://doi.org/10.1186/s13619-022-00137-4","url":null,"abstract":"Intestinal organoids, derived from intestinal stem cell self-organization, recapitulate the tissue structures and behaviors of the intestinal epithelium, which hold great potential for the study of developmental biology, disease modeling, and regenerative medicine. The intestinal epithelium is exposed to dynamic mechanical forces which exert profound effects on gut development. However, the conventional intestinal organoid culture system neglects the key role of mechanical microenvironments but relies solely on biological factors. Here, we show that adding cyclic stretch to intestinal organoid cultures remarkably up-regulates the signature gene expression and proliferation of intestinal stem cells. Furthermore, mechanical stretching stimulates the expansion of SOX9+ progenitors by activating the Wnt/β-Catenin signaling. These data demonstrate that the incorporation of mechanical stretch boosts the stemness of intestinal stem cells, thus benefiting organoid growth. Our findings have provided a way to optimize an organoid generation system through understanding cross-talk between biological and mechanical factors, paving the way for the application of mechanical forces in organoid-based models.","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":" ","pages":"39"},"PeriodicalIF":0.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9626719/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40659610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Glioma stem cells and neural stem cells respond differently to BMP4 signaling. 胶质瘤干细胞和神经干细胞对BMP4信号的反应不同。

Cell Regeneration Pub Date : 2022-11-01 DOI: 10.1186/s13619-022-00136-5

Xin-Xin Han, Chunhui Cai, Li-Ming Yu, Min Wang, Wenhan Yang, Dai-Yu Hu, Jie Ren, Lu-Ying Zhu, Jia-Jia Deng, Qing-Qing Chen, Hua He, Zhengliang Gao

{"title":"Glioma stem cells and neural stem cells respond differently to BMP4 signaling.","authors":"Xin-Xin Han, Chunhui Cai, Li-Ming Yu, Min Wang, Wenhan Yang, Dai-Yu Hu, Jie Ren, Lu-Ying Zhu, Jia-Jia Deng, Qing-Qing Chen, Hua He, Zhengliang Gao","doi":"10.1186/s13619-022-00136-5","DOIUrl":"https://doi.org/10.1186/s13619-022-00136-5","url":null,"abstract":"Malignant glioma is a highly heterogeneous and invasive primary brain tumor characterized by high recurrence rates, resistance to combined therapy, and dismal prognosis. Glioma stem cells (GSCs) are likely responsible for tumor progression, resistance to therapy, recurrence, and poor prognosis owing to their high self-renewal and tumorigenic potential. As a family member of BMP signaling, bone morphogenetic protein4 (BMP4) has been reported to induce the differentiation of GSCs and neural stem cells (NSCs). However, the molecular mechanisms underlying the BMP4-mediated effects in these two cell types are unclear. In this study, we treated hGSCs and hNSCs with BMP4 and compared the phenotypic and transcriptional changes between these two cell types. Phenotypically, we found that the growth of hGSCs was greatly inhibited by BMP4, but the same treatment only increased the cell size of hNSCs. While the RNA sequencing results showed that BMP4 treatment evoked significantly transcriptional changes in both hGSCs and hNSCs, the profiles of differentially expressed genes were distinct between the two groups. A gene set that specifically targeted the proliferation and differentiation of hGSCs but not hNSCs was enriched and then validated in hGSC culture. Our results suggested that hGSCs and hNSCs responded differently to BMP4 stimulation. Understanding and investigating different responses between hGSCs and hNSCs will benefit finding partner factors working together with BMP4 to further suppress GSCs proliferation and stemness without disturbing NSCs.","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":" ","pages":"36"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9622962/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40658938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-resolution single-cell analysis paves the cellular path for brain regeneration in salamanders. 高分辨率单细胞分析为蝾螈的大脑再生铺平了细胞路径。

Cell Regeneration Pub Date : 2022-10-19 DOI: 10.1186/s13619-022-00144-5

Binxu Yin, Xinyun Li, Gufa Lin, Heng Wang

引用次数: 0

Microfluidic devices as model platforms of CNS injury-ischemia to study axonal regeneration by regulating mitochondrial transport and bioenergetic metabolism. 微流控装置作为中枢神经系统损伤缺血模型平台，通过调节线粒体运输和生物能量代谢来研究轴突再生。

Cell Regeneration Pub Date : 2022-10-03 DOI: 10.1186/s13619-022-00138-3

Ning Huang, Zu-Hang Sheng

{"title":"Microfluidic devices as model platforms of CNS injury-ischemia to study axonal regeneration by regulating mitochondrial transport and bioenergetic metabolism.","authors":"Ning Huang, Zu-Hang Sheng","doi":"10.1186/s13619-022-00138-3","DOIUrl":"https://doi.org/10.1186/s13619-022-00138-3","url":null,"abstract":"Central nervous system (CNS) neurons typically fail to regenerate their axons after injury leading to neurological impairment. Axonal regeneration is a highly energy-demanding cellular program that requires local mitochondria to supply most energy within injured axons. Recent emerging lines of evidence have started to reveal that injury-triggered acute mitochondrial damage and local energy crisis contribute to the intrinsic energetic restriction that accounts for axon regeneration failure in the CNS. Characterizing and reprogramming bioenergetic signaling and mitochondrial maintenance after axon injury-ischemia is fundamental for developing therapeutic strategies that can restore local energy metabolism and thus facilitate axon regeneration. Therefore, establishing reliable and reproducible neuronal model platforms is critical for assessing axonal energetic metabolism and regeneration capacity after injury-ischemia. In this focused methodology article, we discuss recent advances in applying cutting-edge microfluidic chamber devices in combination with state-of-the-art live-neuron imaging tools to monitor axonal regeneration, mitochondrial transport, bioenergetic metabolism, and local protein synthesis in response to injury-ischemic stress in mature CNS neurons.","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":" ","pages":"33"},"PeriodicalIF":0.0,"publicationDate":"2022-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9527262/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40386465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A mouse model of vitiligo based on endogenous auto-reactive CD8 + T cell targeting skin melanocyte. 基于内源性自身反应性CD8 + T细胞靶向皮肤黑素细胞的小鼠白癜风模型。

Cell Regeneration Pub Date : 2022-10-02 DOI: 10.1186/s13619-022-00132-9

Daoming Chen, Zijian Xu, Jun Cui, Ting Chen

{"title":"A mouse model of vitiligo based on endogenous auto-reactive CD8 + T cell targeting skin melanocyte.","authors":"Daoming Chen, Zijian Xu, Jun Cui, Ting Chen","doi":"10.1186/s13619-022-00132-9","DOIUrl":"https://doi.org/10.1186/s13619-022-00132-9","url":null,"abstract":"Vitiligo is the most common human skin depigmenting disorder. It is mediated by endogenous autoreactive CD8 + T cells that destruct skin melanocytes. This disease has an estimated prevalence of 1% of the global population and currently has no cure. Animal models are indispensable tools for understanding vitiligo pathogenesis and for developing new therapies. Here, we describe a vitiligo mouse model which recapitulates key clinical features of vitiligo, including epidermis depigmentation, CD8 + T cell infiltration in skin, and melanocyte loss. To activate endogenous autoreactive cytotoxic CD8 + T cells targeting melanocytes, this model relies on transient inoculation of B16F10 melanoma cells and depletion of CD4 + regulatory T cells. At cellular level, epidermal CD8 + T cell infiltration and melanocyte loss start as early as Day 19 after treatment. Visually apparent epidermis depigmentation occurs 2 months later. This protocol can efficiently induce vitiligo in any C57BL/6 background mouse strain, using only commercially available reagents. This enables researchers to carry out in-depth in vivo vitiligo studies utilizing mouse genetics tools, and provides a powerful platform for drug discovery.","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":" ","pages":"31"},"PeriodicalIF":0.0,"publicationDate":"2022-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9526765/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40388653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Cilia regeneration requires an RNA splicing factor from the ciliary base. 纤毛再生需要来自纤毛基部的RNA剪接因子。

Cell Regeneration Pub Date : 2022-10-01 DOI: 10.1186/s13619-022-00130-x

Kaiming Xu, Guangshuo Ou

引用次数: 1

CTCF acetylation at lysine 20 is required for the early cardiac mesoderm differentiation of embryonic stem cells. CTCF赖氨酸20乙酰化是胚胎干细胞早期心脏中胚层分化所必需的。

Cell Regeneration Pub Date : 2022-09-19 DOI: 10.1186/s13619-022-00131-w

Shixin Gong, Gongcheng Hu, Rong Guo, Jie Zhang, Yiqi Yang, Binrui Ji, Gang Li, Hongjie Yao

引用次数: 1

Rise of the human-mouse chimeric brain models. 人鼠嵌合脑模型的兴起。

Cell Regeneration Pub Date : 2022-09-03 DOI: 10.1186/s13619-022-00135-6

Peng Jiang, Mahabub Maraj Alam

引用次数: 3

The therapeutic prospects and challenges of human neural stem cells for the treatment of Alzheimer's Disease. 人类神经干细胞治疗阿尔茨海默病的前景和挑战。

Cell Regeneration Pub Date : 2022-09-02 DOI: 10.1186/s13619-022-00128-5

Chunmei Yue, Su Feng, Yingying Chen, Naihe Jing

{"title":"The therapeutic prospects and challenges of human neural stem cells for the treatment of Alzheimer's Disease.","authors":"Chunmei Yue, Su Feng, Yingying Chen, Naihe Jing","doi":"10.1186/s13619-022-00128-5","DOIUrl":"https://doi.org/10.1186/s13619-022-00128-5","url":null,"abstract":"Alzheimer's disease (AD) is a multifactorial neurodegenerative disorder associated with aging. Due to its insidious onset, protracted progression, and unclear pathogenesis, it is considered one of the most obscure and intractable brain disorders, and currently, there are no effective therapies for it. Convincing evidence indicates that the irreversible decline of cognitive abilities in patients coincides with the deterioration and degeneration of neurons and synapses in the AD brain. Human neural stem cells (NSCs) hold the potential to functionally replace lost neurons, reinforce impaired synaptic networks, and repair the damaged AD brain. They have therefore received extensive attention as a possible source of donor cells for cellular replacement therapies for AD. Here, we review the progress in NSC-based transplantation studies in animal models of AD and assess the therapeutic advantages and challenges of human NSCs as donor cells. We then formulate a promising transplantation approach for the treatment of human AD, which would help to explore the disease-modifying cellular therapeutic strategy for the treatment of human AD.","PeriodicalId":9811,"journal":{"name":"Cell Regeneration","volume":" ","pages":"28"},"PeriodicalIF":0.0,"publicationDate":"2022-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40340536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4