在人类启动的幼稚多能性转换过程中,染色质可及性和转录活性之间的不一致。

IF 4 Q2 CELL & TISSUE ENGINEERING
Zhifen Tu, Yan Bi, Tengyan Mao, Hong Wang, Shaorong Gao, Yixuan Wang
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引用次数: 2

摘要

Naïve多能状态可以通过多种策略从各种类型的细胞中获得,其中详细描述了细胞命运路线图以及旅程中涉及的关键生物事件。在这里,我们通过采用双荧光报告子系统和转座酶可及染色质(ATAC)-seq测定,仔细探索了从启动到幼稚转变期间的染色质可及性动力学。我们的结果揭示了关键的染色质重塑事件,并强调了染色质可及性和转录活性之间的不一致。我们进一步证明,不同的表观遗传学修饰和转录因子(TF)活性可能在调节基因表达中发挥关键作用,并解释了尽管染色质景观相似,但观察到的基因表达变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Discordance between chromatin accessibility and transcriptional activity during the human primed-to-naïve pluripotency transition process.

Naïve pluripotent state can be obtained by several strategies from various types of cells, in which the cell fate roadmap as well as key biological events involved in the journey have been described in detail. Here, we carefully explored the chromatin accessibility dynamics during the primed-to-naïve transition by adopting a dual fluorescent reporter system and the assay for transposase-accessible chromatin (ATAC)-seq. Our results revealed critical chromatin remodeling events and highlight the discordance between chromatin accessibility and transcriptional activity. We further demonstrate that the differential epigenetic modifications and transcription factor (TF) activities may play a critical role in regulating gene expression, and account for the observed variations in gene expression despite similar chromatin landscapes.

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来源期刊
Cell Regeneration
Cell Regeneration Biochemistry, Genetics and Molecular Biology-Cell Biology
CiteScore
5.80
自引率
0.00%
发文量
42
审稿时长
35 days
期刊介绍: Cell Regeneration aims to provide a worldwide platform for researches on stem cells and regenerative biology to develop basic science and to foster its clinical translation in medicine. Cell Regeneration welcomes reports on novel discoveries, theories, methods, technologies, and products in the field of stem cells and regenerative research, the journal is interested, but not limited to the following topics: ◎ Embryonic stem cells ◎ Induced pluripotent stem cells ◎ Tissue-specific stem cells ◎ Tissue or organ regeneration ◎ Methodology ◎ Biomaterials and regeneration ◎ Clinical translation or application in medicine
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