Zhifen Tu, Yan Bi, Tengyan Mao, Hong Wang, Shaorong Gao, Yixuan Wang
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Discordance between chromatin accessibility and transcriptional activity during the human primed-to-naïve pluripotency transition process.
Naïve pluripotent state can be obtained by several strategies from various types of cells, in which the cell fate roadmap as well as key biological events involved in the journey have been described in detail. Here, we carefully explored the chromatin accessibility dynamics during the primed-to-naïve transition by adopting a dual fluorescent reporter system and the assay for transposase-accessible chromatin (ATAC)-seq. Our results revealed critical chromatin remodeling events and highlight the discordance between chromatin accessibility and transcriptional activity. We further demonstrate that the differential epigenetic modifications and transcription factor (TF) activities may play a critical role in regulating gene expression, and account for the observed variations in gene expression despite similar chromatin landscapes.
Cell RegenerationBiochemistry, Genetics and Molecular Biology-Cell Biology
CiteScore
5.80
自引率
0.00%
发文量
42
审稿时长
35 days
期刊介绍:
Cell Regeneration aims to provide a worldwide platform for researches on stem cells and regenerative biology to develop basic science and to foster its clinical translation in medicine. Cell Regeneration welcomes reports on novel discoveries, theories, methods, technologies, and products in the field of stem cells and regenerative research, the journal is interested, but not limited to the following topics:
◎ Embryonic stem cells
◎ Induced pluripotent stem cells
◎ Tissue-specific stem cells
◎ Tissue or organ regeneration
◎ Methodology
◎ Biomaterials and regeneration
◎ Clinical translation or application in medicine