Cell Proliferation最新文献_第5页

TREM2 Impedes Recovery After Spinal Cord Injury by Regulating Microglial Lysosomal Membrane Permeabilization-Mediated Autophagy. TREM2通过调节小胶质溶酶体膜渗透介导的自噬阻碍脊髓损伤后的恢复。

IF 5.9 1区生物学

Cell Proliferation Pub Date : 2025-05-04 DOI: 10.1111/cpr.70047

Tianlun Zhao, Jiawei Di, Yu Kang, Haojie Zhang, Senyu Yao, Bin Liu, Limin Rong

{"title":"TREM2 Impedes Recovery After Spinal Cord Injury by Regulating Microglial Lysosomal Membrane Permeabilization-Mediated Autophagy.","authors":"Tianlun Zhao, Jiawei Di, Yu Kang, Haojie Zhang, Senyu Yao, Bin Liu, Limin Rong","doi":"10.1111/cpr.70047","DOIUrl":"https://doi.org/10.1111/cpr.70047","url":null,"abstract":"Microglia, considered as the main immune responder, play an important role in regulating neuroinflammation in central nervous system (CNS) disorders. Our previous work found that TREM2 is highly expressed in microglia and is related to their functional state. However, the specific role of TREM2 in spinal cord injury has not yet been explored. To further investigate the potential mechanism of TREM2, we performed single-cell sequencing on wild-type (Wt) and Trem2-/- mice before and after spinal cord injury. Compared to Wt mice, the lysosome, autophagy and membrane-related pathways are more strongly activated in Trem2-/- mice, suggesting that TREM2 may exert its effects by influencing lysosomal membranes and autophagy. Mechanistically, we demonstrated that the knockout of Trem2 can reduce the nuclear translocation of TFEB by decreasing the phosphorylation of Syk. Furthermore, we validated that in vitro and in vivo silencing Trem2 can promote autophagy by repairing lysosomal membrane permeabilization. Through immunofluorescence, 3D gait analysis, motor evoked potential experiments, H&E staining and Masson staining, we demonstrated that the increased level of autophagy can rescue more microglia in vivo and promote both functional and histological recovery of spinal cord injury. Collectively, these results not only suggest that microglial lysosomal autophagy is regulated in a TREM2-dependent LMP manner, but also, more importantly, they provide a promising clinical translation strategy based on gene therapy for lysosome-related central nervous system disorders.","PeriodicalId":9760,"journal":{"name":"Cell Proliferation","volume":" ","pages":"e70047"},"PeriodicalIF":5.9,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hypertranscription of rDNA Responsible for Nucleolar Remodelling is a Doorman for Acquiring Pluripotency. 负责核仁重塑的rDNA的超转录是获得多能性的关键。

IF 5.9 1区生物学

Cell Proliferation Pub Date : 2025-05-04 DOI: 10.1111/cpr.70052

Yuchen Sun, Xinglin Hu, Xingwei Huang, Wenyao Zhou, Shubing Lan, Hui Zhang, Guangming Wu, Lei Lei

{"title":"Hypertranscription of rDNA Responsible for Nucleolar Remodelling is a Doorman for Acquiring Pluripotency.","authors":"Yuchen Sun, Xinglin Hu, Xingwei Huang, Wenyao Zhou, Shubing Lan, Hui Zhang, Guangming Wu, Lei Lei","doi":"10.1111/cpr.70052","DOIUrl":"https://doi.org/10.1111/cpr.70052","url":null,"abstract":"Ribosome biogenesis occurs within the nucleolus, with the initial step being the transcription of ribosomal DNA (rDNA). Although rDNA transcription is limited in somatic cells, it is more active in stem cells. Nevertheless, the mechanisms involved in somatic cell reprogramming remain elusive. Both somatic and stem cell nucleoli exhibit a reticular structure. However, under the electron microscope, we identified an intermediate nucleolar state during reprogramming. This state underwent changes characterised by rDNA hypertranscription, resulting in an enlarged nucleolus, enhanced activity of nucleolus organiser regions (NORs), and a transition from the reticular nucleolar type to an intermediate state of reprogramming, whose three liquid phase boundaries are blurred. Our research revealed that Oct4 was directly targeted to the rDNA enhancer region, promoting its hypertranscription and nucleolar enlargement during reprogramming. Using rDNA transcriptional inhibitors, we proved that nucleolar remodelling and subsequent reprogramming are halted by inhibiting rDNA transcription. But why could rDNA transcriptional activity influence reprogramming? Our findings elucidate that the active nucleoli have the capability to release perinucleolar heterochromatin. By joint analysis of Assay for Transposase-Accessible Chromatin with high throughput sequencing (ATAC-seq) and RNA-seq, we have characterised the perinucleolar chromatin released by the nucleolus in a reprogramming intermediate state. The released chromatin mainly impacted mesenchymal-to-epithelial transition (MET)-related genes. MET is a stage of silencing of mesenchymal genes, accompanied by the activation of epithelial genes. Concurrently, the morphology of mouse embryonic fibroblast cells (MEFs) transitions from elongated spindle-shaped cells to short roundish forms, exhibiting a propensity to cluster together. MET was considered an early event in reprogramming; our findings suggested that nucleolar remodelling occurred before MET.","PeriodicalId":9760,"journal":{"name":"Cell Proliferation","volume":" ","pages":"e70052"},"PeriodicalIF":5.9,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143974445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

THER: Integrative Web Tool for Tumour Hypoxia Exploration and Research. THER：肿瘤缺氧探索和研究的综合网络工具。

IF 5.9 1区生物学

Cell Proliferation Pub Date : 2025-05-01 DOI: 10.1111/cpr.70053

Yasi Zhang, Huiying Liu, Pengpeng Zhang, BiCheng Ye, Haoxuan Ying, Hong Yang, Jian Zhang, Nan Zhang, Kailai Li, Ting Wei, Aimin Jiang, Anqi Lin, Peng Luo

引用次数: 0

Mechanistic Insights and Therapeutic Potentials of Ubiquitin-Proteasome System in Non-Small Cell Lung Cancer 泛素-蛋白酶体系统在非小细胞肺癌中的作用机制及治疗潜力。

IF 5.9 1区生物学

Cell Proliferation Pub Date : 2025-05-01 DOI: 10.1111/cpr.70050

Guangyao Zhou, Jiaxiong Tan, Pengpeng Zhang, Zhaokai Zhou, Lianmin Zhang, Zhenfa Zhang

{"title":"Mechanistic Insights and Therapeutic Potentials of Ubiquitin-Proteasome System in Non-Small Cell Lung Cancer","authors":"Guangyao Zhou, Jiaxiong Tan, Pengpeng Zhang, Zhaokai Zhou, Lianmin Zhang, Zhenfa Zhang","doi":"10.1111/cpr.70050","DOIUrl":"10.1111/cpr.70050","url":null,"abstract":"Non-small cell lung cancer (NSCLC) remains a leading cause of cancer mortality. Despite advancements in gene targeted therapies and immunotherapies, high heterogeneity contributes to limited efficacy and therapeutic resistance. Ubiquitination, a crucial post-translational modification that regulates protein stability and degradation, plays a significant role in cancer pathogenesis by influencing key oncogenic pathways and tumour progression. This review systematically explores the ubiquitin-proteasome system (UPS) and its potential as a therapeutic target for NSCLC. We highlight recent preclinical and clinical studies focusing on ubiquitination-related biomarkers, drug targets and emerging therapies like proteasome inhibitors and Proteolysis-targeting chimeras (PROTACs). By exploring the impact of the UPS on tumour biology, the progression of NSCLC and its response to therapy, we aim to underscore the potential of targeting the ubiquitination-deubiquitination system as a complementary or synergistic approach to existing therapeutic strategies in NSCLC, thereby enhancing patient outcomes and overcoming treatment resistance.","PeriodicalId":9760,"journal":{"name":"Cell Proliferation","volume":"58 7","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/cpr.70050","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143982192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GANT61 Modulates Autophagy and Lipid Metabolism in Ovarian Cancer GANT61调节卵巢癌自噬和脂质代谢。

IF 5.9 1区生物学

Cell Proliferation Pub Date : 2025-05-01 DOI: 10.1111/cpr.70051

Yibin Pan, Lingfeng Chen, Jinlu Shen, Shihao Hong, Xiaojing Guan, Xudong Ma, Rongrong Tang, Meifei Lu, Fangying Sun, Shanliang Shang, Yongdong Dai, Zhaokai Zhou, Songying Zhang, Jianhua Yang

引用次数: 0

Synergistical Induction of Apoptosis via Cold Atmospheric Plasma and Nanohydroxyapatite for Selective Inhibition of Oral Squamous Cell Carcinoma in Tumour Microenvironment. 低温大气等离子体和纳米羟基磷灰石协同诱导细胞凋亡选择性抑制口腔鳞状细胞癌肿瘤微环境。

IF 5.9 1区生物学

Cell Proliferation Pub Date : 2025-04-29 DOI: 10.1111/cpr.70041

Wenting Qi, Hanghang Liu, Huaze Liu, Yuxuan Guo, Li Wu, Chongyun Bao, Xian Liu

{"title":"Synergistical Induction of Apoptosis via Cold Atmospheric Plasma and Nanohydroxyapatite for Selective Inhibition of Oral Squamous Cell Carcinoma in Tumour Microenvironment.","authors":"Wenting Qi, Hanghang Liu, Huaze Liu, Yuxuan Guo, Li Wu, Chongyun Bao, Xian Liu","doi":"10.1111/cpr.70041","DOIUrl":"https://doi.org/10.1111/cpr.70041","url":null,"abstract":"Surgical resection, radiotherapy and chemotherapy are the primary strategies of treating cancers globally. However, the current treatment methods bring new disease burdens to patients due to postoperative complications and multiple side effects, especially in surface tumours such as oral squamous cell carcinoma (OSCC). In this study, we developed a microwave cold atmospheric plasma (CAP) device in conjunction with tumour microenvironment-responsive nanohydroxyapatite (nHA) for the first time. The synergistic effects of CAP and nHA combined application on OSCC were evaluated in both in vitro and in vivo experiments. The synergistic effects of CAP and pH-responsive NH2-nHA on the apoptosis, intracellular reactive oxygen species (ROS) and calcium ion concentration of OSCC cells were investigated in vitro. The synergistic induction of CAP with NH2-nHA exhibited optimal tumour-specific inhibitory effects on OSCC. The results revealed that the combined application of CAP with NH2-nHA induced apoptosis of tumour cells in vitro and killed 84.0% of tumours in vivo. Mechanistically, CAP enhances extracellular ROS production, while NH2-nHA amplifies intracellular calcium ion (Ca2+) concentrations, synergistically increasing intracellular ROS levels to provoke oxidative stress in OSCC cells, ultimately triggering the mitochondrial apoptosis pathway. In conclusion, the combined utilisation of CAP and NH2-nHA presents a promising avenue as a novel, selective, and non-invasive strategy in the management of OSCC.","PeriodicalId":9760,"journal":{"name":"Cell Proliferation","volume":" ","pages":"e70041"},"PeriodicalIF":5.9,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143981687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extrachromosomal Circular DNA in Cancer: Mechanisms and Clinical Applications 染色体外环状DNA在癌症中的作用：机制和临床应用。

IF 5.9 1区生物学

Cell Proliferation Pub Date : 2025-04-29 DOI: 10.1111/cpr.70040

Jiajia Li, Peng Luo, Zhengrui Li, Qi Wang, Xufeng Huang, Keliang Wang, Ruo Wang, Runzhi Chen

引用次数: 0

Mechanical Force Regulates the Paracrine Function of ADSCs to Promote the Adipose-Regenerating Effects of AAM by Regulating Angiogenesis and the Inflammatory Response 机械力调节ADSCs的旁分泌功能，通过调节血管生成和炎症反应促进AAM的脂肪再生作用

IF 5.9 1区生物学

Cell Proliferation Pub Date : 2025-04-27 DOI: 10.1111/cpr.70045

Yining Wang, Luyu Zhang, Jiaxuan Liu, Yuchao Yang, Zhenyu Bi, Jun Ouyang

引用次数: 0

Elucidating the Role and Mechanism of Alpha-Enolase in Senescent Amelioration via Metabolic Reprogramming. α -烯醇化酶通过代谢重编程改善衰老的作用和机制。

IF 5.9 1区生物学

Cell Proliferation Pub Date : 2025-04-27 DOI: 10.1111/cpr.70049

Yun Haeng Lee, Hyunwoong Lim, Gyungmin Kim, Geonhee Jang, Myeong Uk Kuk, Ji Ho Park, Jee Hee Yoon, Yoo Jin Lee, Duyeol Kim, Byeonghyeon So, Minseon Kim, Hyung Wook Kwon, Youngjoo Byun, Joon Tae Park

{"title":"Elucidating the Role and Mechanism of Alpha-Enolase in Senescent Amelioration via Metabolic Reprogramming.","authors":"Yun Haeng Lee, Hyunwoong Lim, Gyungmin Kim, Geonhee Jang, Myeong Uk Kuk, Ji Ho Park, Jee Hee Yoon, Yoo Jin Lee, Duyeol Kim, Byeonghyeon So, Minseon Kim, Hyung Wook Kwon, Youngjoo Byun, Joon Tae Park","doi":"10.1111/cpr.70049","DOIUrl":"https://doi.org/10.1111/cpr.70049","url":null,"abstract":"Senescent cells are characterised by increased glycolysis dependence. Normalisation of glycolysis metabolism is essential for senescence amelioration. However, the mechanism of proteins involved in cellular glycolysis metabolism has not been fully elucidated. Here, we identified a candidate compound, an oxazole analogue (KB2764), that can improve senescence. To elucidate the mechanism of the KB2764, we investigated the interacting proteins. KB2764 interacted with alpha-enolase (ENO1) and pyruvate kinase M (PKM), ultimately allowing PKM to phosphorylate ENO1. KB2764 consequently increased mitochondrial ATP production and reduced reliance on glycolysis. Knockdown of the ENO1 experiment in senescent cells demonstrates that regulation of ENO1 activity is a prerequisite for recovery of mitochondrial function. Furthermore, the action of KB2764 extends its application to extend the lifespan of Caenorhabditis elegans. Taken together, our findings reveal a novel mechanism by which senescence is ameliorated through metabolic reprogramming and mitochondrial functional recovery via KB2764-mediated regulation of ENO1 protein activity.","PeriodicalId":9760,"journal":{"name":"Cell Proliferation","volume":" ","pages":"e70049"},"PeriodicalIF":5.9,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intelligent Manufacturing for Osteoarthritis Organoids 骨关节炎类器官的智能制造。

IF 5.9 1区生物学

Cell Proliferation Pub Date : 2025-04-26 DOI: 10.1111/cpr.70043

Xukun Lyu, Jian Wang, Jiacan Su

{"title":"Intelligent Manufacturing for Osteoarthritis Organoids","authors":"Xukun Lyu, Jian Wang, Jiacan Su","doi":"10.1111/cpr.70043","DOIUrl":"10.1111/cpr.70043","url":null,"abstract":"Osteoarthritis (OA) is the most prevalent degenerative joint disease worldwide, imposing a substantial global disease burden. However, its pathogenesis remains incompletely understood, and effective treatment strategies are still lacking. Organoid technology, in which stem cells or progenitor cells self-organise into miniature tissue structures under three-dimensional (3D) culture conditions, provides a promising in vitro platform for simulating the pathological microenvironment of OA. This approach can be employed to investigate disease mechanisms, carry out high-throughput drug screening and facilitate personalised therapies. This review summarises joint structure, OA pathogenesis and pathological manifestations, thereby establishing the disease context for the application of organoid technology. It then examines the components of the arthrosis organoid system, specifically addressing cartilage, subchondral bone, synovium, skeletal muscle and ligament organoids. Furthermore, it details various strategies for constructing OA organoids, including considerations of cell selection, pathological classification and fabrication techniques. Notably, this review introduces the concept of intelligent manufacturing of OA organoids by incorporating emerging engineering technologies such as artificial intelligence (AI) into the organoid fabrication process, thereby forming an innovative software and hardware cluster. Lastly, this review discusses the challenges currently facing intelligent OA organoid manufacturing and highlights future directions for this rapidly evolving field. By offering a comprehensive overview of state-of-the-art methodologies and challenges, this review anticipates that intelligent, automated fabrication of OA organoids will expedite fundamental research, drug discovery and personalised translational applications in the orthopaedic field.","PeriodicalId":9760,"journal":{"name":"Cell Proliferation","volume":"58 7","pages":""},"PeriodicalIF":5.9,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/cpr.70043","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143974446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0