Cell Proliferation最新文献_第4页

Correction to "Shiga-Like Toxin I Exerts Specific and Potent Anti-Tumour Efficacy Against Gastric Cancer Cell Proliferation When Driven by Tumour-Preferential Frizzled-7 Promoter". 更正“志贺样毒素I在肿瘤优先型卷曲-7启动子驱动下对胃癌细胞增殖具有特异性和强效的抗肿瘤作用”。

IF 5.9 1区生物学

Cell Proliferation Pub Date : 2025-04-01 DOI: 10.1111/cpr.70030

引用次数: 0

Scalable Matrigel-Free Suspension Culture for Generating High-Quality Human Liver Ductal Organoids. 可扩展无基质悬浮培养制备高质量人肝导管类器官。

IF 5.9 1区生物学

Cell Proliferation Pub Date : 2025-04-01 DOI: 10.1111/cpr.70033

Senyi Gong, Kangxin He, Yu Liu, Xingyu Luo, Kamran Ashraf, Jinzhao He, Weifeng Li, Lihua Yang, Touseef Ur Rehman, Mingwei Shen, Qinbiao Yan, Ali Mohsin, Shusen Zheng, Zhe Yang, Meijin Guo

{"title":"Scalable Matrigel-Free Suspension Culture for Generating High-Quality Human Liver Ductal Organoids.","authors":"Senyi Gong, Kangxin He, Yu Liu, Xingyu Luo, Kamran Ashraf, Jinzhao He, Weifeng Li, Lihua Yang, Touseef Ur Rehman, Mingwei Shen, Qinbiao Yan, Ali Mohsin, Shusen Zheng, Zhe Yang, Meijin Guo","doi":"10.1111/cpr.70033","DOIUrl":"https://doi.org/10.1111/cpr.70033","url":null,"abstract":"Liver transplantation is currently the sole definitive treatment option for end-stage liver failure. However, a significant shortage of donors prevails due to high clinical demands. Recently, human liver organoids have shown significant potential in regenerative medicine for liver diseases. Nevertheless, current static cultures of organoids grown in well-plates heavily rely on extracellular matrix hydrogels (Matrigel), thereby limiting both the scalability and quantity of organoid culture. In this study, we present a groundbreaking culture mode that eliminates all reliance on extracellular matrix hydrogels, enabling the successful preparation of functional human liver ductal organoids (LDOs) based on the cell suspension culture mode in a mechanically stirred bioreactor. Initially, the developed suspension culture in a 6-well plate without matrigel was proven to support robust growth of liver ductal organoids with an average size 2.6 times larger than those obtained in static culture, and with a high organoid survival rate exceeding 90%. Also, the transcriptome profile reveals that suspension culture activates the phosphatidylinositol 3-kinase (PI3K) signalling pathway through mechanical signal transduction, thereby promoting hepatobiliary characteristics. Then, a controllable and scalable bioprocess for liver ductal organoid culture was developed and successfully scaled up to a 50 mL flask bioreactor with a working volume of 15 mL. Finally, animal experiments indicated that the transplantation of liver ductal organoids harvested from suspension culture can effectively alleviate liver injury and inflammation, demonstrating the feasibility of large-scale production of liver ductal organoids cultivated in suspension culture with an improved extracellular matrix environment.","PeriodicalId":9760,"journal":{"name":"Cell Proliferation","volume":" ","pages":"e70033"},"PeriodicalIF":5.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143751341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting SHP-1-Mediated Inhibition of STAT3 and ERK Signalling Pathways Rescues the Hyporesponsiveness of MHC-I-Deficient NK-92MI. 靶向shp -1介导的STAT3和ERK信号通路抑制可拯救mhc - i缺陷NK-92MI的低反应性

IF 5.9 1区生物学

Cell Proliferation Pub Date : 2025-04-01 DOI: 10.1111/cpr.70035

Kuo Yu, Xiaolong Liu, Guangyuan Wu, Zhongyao An, Xin Wang, Yang Liu, Hailong Wang, Mingli Huang, Linlin Zhao, Ce Shi, Xin Sun, Lu Xu, Sen Qi, Xin Zhang, Yueqiu Teng, Song Guo Zheng, Zhiren Zhang, Zhenkun Wang

{"title":"Targeting SHP-1-Mediated Inhibition of STAT3 and ERK Signalling Pathways Rescues the Hyporesponsiveness of MHC-I-Deficient NK-92MI.","authors":"Kuo Yu, Xiaolong Liu, Guangyuan Wu, Zhongyao An, Xin Wang, Yang Liu, Hailong Wang, Mingli Huang, Linlin Zhao, Ce Shi, Xin Sun, Lu Xu, Sen Qi, Xin Zhang, Yueqiu Teng, Song Guo Zheng, Zhiren Zhang, Zhenkun Wang","doi":"10.1111/cpr.70035","DOIUrl":"https://doi.org/10.1111/cpr.70035","url":null,"abstract":"Natural Killer (NK) cells have shown promising prospects in 'off-the-shelf' cell therapy, particularly the NK-92 cell line, which can serve as a foundation for the next generation of universal chimeric antigen receptor (CAR)-engineered NK products. A key strategy for generating universal cellular products is the elimination of the beta-2-microglobulin (B2M) gene, which encodes a component of MHC class I molecules (MHC-I) that plays a role in the presentation of foreign antigens and in the 'licensing' or 'education' of NK cells. To functionally study the impacts of MHC-I deficiency on NK-92, we generated a B2M knockout (KO) NK-92MI (B-92) cell line and compared the multidimensional properties of B2M KO and wild-type NK-92MI cells in terms of biological phenotypes, effector functions, and transcriptomic signatures. We observed a decrease in activating receptors, cytokine production, and cytotoxicity in B-92 cells. Further analysis of signalling events revealed that the upregulated expression and phosphorylation of SHP-1 in B-92 cells inhibited the phosphorylation levels of STAT3 and ERK, thereby affecting their killing function. By knocking out SHP-1 (PTPN6), we partially restored the cytotoxic function of B-92 cells. Notably, we also found that CAR modification can overcome the hyporesponsiveness of B-92 cells. These findings will facilitate further exploration in the development of NK cell-based products.","PeriodicalId":9760,"journal":{"name":"Cell Proliferation","volume":" ","pages":"e70035"},"PeriodicalIF":5.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143751345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accelerating Bone Healing With METTL3 Overexpressed Adipose-Derived Stem Cells in Osteoporotic Rats. METTL3过表达脂肪来源干细胞加速骨质疏松大鼠骨愈合

IF 5.9 1区生物学

Cell Proliferation Pub Date : 2025-03-24 DOI: 10.1111/cpr.70029

Hui Tang, Zhenzhen Chen, Lu Zeng, Yuping Xie, Daowen Luo, Shuanglin Peng, Fangzhi Lou, Tianli Wu, Jingang Xiao

引用次数: 0

Exercise Delays Brain Ageing Through Muscle-Brain Crosstalk. 运动通过肌肉-大脑的相互作用延缓大脑衰老。

IF 5.9 1区生物学

Cell Proliferation Pub Date : 2025-03-24 DOI: 10.1111/cpr.70026

Shirin Pourteymour, Rakesh Kumar Majhi, Frode A Norheim, Christian A Drevon

引用次数: 0

Utilising Human Myometrial and Uterine Fibroid Stem Cell-Derived Three Dimentional Organoids as a Robust Model System for Understanding the Pathophysiology of Uterine Fibroids. 利用人子宫肌瘤和子宫肌瘤干细胞衍生的三维类器官作为了解子宫肌瘤病理生理的稳健模型系统。

IF 5.9 1区生物学

Cell Proliferation Pub Date : 2025-03-20 DOI: 10.1111/cpr.70025

Mervat M Omran, Somayeh Vafaei, Samar Alkhrait, Farzana Liakath Ali, Maria Victoria Bariani, Tao Bai, Winston E Thompson, Qiwei Yang, Mohamed Ali, Ayman Al-Hendy

{"title":"Utilising Human Myometrial and Uterine Fibroid Stem Cell-Derived Three Dimentional Organoids as a Robust Model System for Understanding the Pathophysiology of Uterine Fibroids.","authors":"Mervat M Omran, Somayeh Vafaei, Samar Alkhrait, Farzana Liakath Ali, Maria Victoria Bariani, Tao Bai, Winston E Thompson, Qiwei Yang, Mohamed Ali, Ayman Al-Hendy","doi":"10.1111/cpr.70025","DOIUrl":"https://doi.org/10.1111/cpr.70025","url":null,"abstract":"Uterine fibroids (UFs) are the most common benign gynecologic tumours affecting women of reproductive age. This study aims to deepen the understanding of UFs complex aetiology through harnessing the power of 3D organoid models derived from human myometrial stem cells to emulate the in vivo behaviour of these tumours. Isolated SCs were cultured over 7 days under a defined culture system. Immunohistochemistry, Immunofluorescence, organoid stiffness, RNA Sequencing was conducted, and differential gene expression was assessed using RT-PCR. The derived organoids exhibited diverse populations of cells, including stem cells, smooth muscle, and fibroblasts. Excessive ECM deposition was shown via Collagen and Fibronectin expression. We confirmed that our organoids expressed oestrogen receptor in a pattern similar to that in their corresponding tissue, as well as responded to steroid hormone. Interestingly, we revealed significant racial disparities in ECM accumulation within organoids derived from different racial groups. This augmented ECM deposition is theorised to enhance tissue stiffness, as assessed using Young's modulus. Additionally, our research demonstrated significant decreases in fibrotic markers upon treatment with Vitamin D3 and Doxercalciferol. Furthermore, the pro-fibroid effects of environmental phthalates further elucidate the potential factors contributing to UF pathology. The 3D organoid model can serve as a robust platform to study the underlying molecular mechanisms of UFs, besides offering invaluable insights for potential therapeutic interventions.","PeriodicalId":9760,"journal":{"name":"Cell Proliferation","volume":" ","pages":"e70025"},"PeriodicalIF":5.9,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GPD1L-Mediated Glycerophospholipid Metabolism Dysfunction in Women With Diminished Ovarian Reserve: Insights From Pseudotargeted Metabolomic Analysis of Follicular Fluid. gpd1l介导的甘油磷脂代谢功能障碍在卵巢储备功能减退的女性：来自卵泡液假靶向代谢组学分析的见解

IF 5.9 1区生物学

Cell Proliferation Pub Date : 2025-03-20 DOI: 10.1111/cpr.70024

Jiaqi Wu, Xuehan Zhao, Ying Fang, Cong Wang, Yichang Tian, Wan Tu, Qiqian Wu, Long Yan, Xiaokui Yang

{"title":"GPD1L-Mediated Glycerophospholipid Metabolism Dysfunction in Women With Diminished Ovarian Reserve: Insights From Pseudotargeted Metabolomic Analysis of Follicular Fluid.","authors":"Jiaqi Wu, Xuehan Zhao, Ying Fang, Cong Wang, Yichang Tian, Wan Tu, Qiqian Wu, Long Yan, Xiaokui Yang","doi":"10.1111/cpr.70024","DOIUrl":"https://doi.org/10.1111/cpr.70024","url":null,"abstract":"Diminished ovarian reserve (DOR) is a pathological condition characterised by reduced ovarian function, which refers to the decreased quality and quantity of oocytes, potentially causing female infertility and various health issues. Follicular fluid (FF) serves as the microenvironment for follicular development and oocyte maturation, gaining an in-depth understanding of the metabolic state of FF will help us uncover the key biological processes involved in ovarian aging, while the specific underlying pathogenic mechanisms are not fully understood. In this study, we utilised pseudotargeted metabolomic analysis of FF to reveal the glycerophospholipid metabolism dysfunction mediated by GPD1L in DOR patients. We also found that GPD1L was downregulated in granulosa cells (GCs) of DOR patients, resulting in increased cell apoptosis and mitochondrial dysfunction. Moreover, our results demonstrated that the downregulated expression of GPD1L could induce follicular atresia and impair oocyte quality in mouse ovaries. Altogether, our research suggested that GPD1L in GCs and the key metabolites in the glycerophospholipid metabolism pathway could potentially act as novel biomarkers of DOR diagnosis, paving the way for a new theoretical basis for understanding the pathogenesis of DOR.","PeriodicalId":9760,"journal":{"name":"Cell Proliferation","volume":" ","pages":"e70024"},"PeriodicalIF":5.9,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

YTHDC1 Modulates the Osteogenic Capacity of hPDLSCs via Wnt/β-Catenin Signalling Pathway for the Treatment of Bone Defects in Osteoporosis Rats. YTHDC1通过Wnt/β-Catenin信号通路调节hPDLSCs成骨能力治疗骨质疏松大鼠骨缺损

IF 5.9 1区生物学

Cell Proliferation Pub Date : 2025-03-17 DOI: 10.1111/cpr.70020

Dan Tan, Qilin Li, Zhenzhen Chen, Hongbing Zhang, Pengcheng Rao, Jingxiang Li, Qianke Tao, Jingang Xiao, Jinlin Song

{"title":"YTHDC1 Modulates the Osteogenic Capacity of hPDLSCs via Wnt/β-Catenin Signalling Pathway for the Treatment of Bone Defects in Osteoporosis Rats.","authors":"Dan Tan, Qilin Li, Zhenzhen Chen, Hongbing Zhang, Pengcheng Rao, Jingxiang Li, Qianke Tao, Jingang Xiao, Jinlin Song","doi":"10.1111/cpr.70020","DOIUrl":"https://doi.org/10.1111/cpr.70020","url":null,"abstract":"Human periodontal ligament stem cells (hPDLSCs) have emerged as promising candidates for the treatment of osteoporotic bone defects. Previous studies have indicated that m6A plays a crucial role in regulating the osteogenic differentiation of hPDLSCs. However, research on the relationship between YTHDC1, as a reading protein, and the osteogenic differentiation of hPDLSCs remains unexplored. This study aimed to investigate the biological roles of YTHDC1 in the osteogenic differentiation of hPDLSCs and to explore underlying mechanisms. Dot blot analysis revealed a progressive increase in m6A methylation during osteogenic differentiation, accompanied by significant upregulation of YTHDC1 expression, as evidenced by qPCR and Western blot. Functional assays utilising siRNA-mediated knockdown and lentiviral-mediated overexpression demonstrated that YTHDC1 positively regulated the osteogenic differentiation potential of hPDLSCs. Mechanistically, mRNA-seq analysis implicated the Wnt/β-catenin signalling pathway, which was further validated through rescue experiments with the Wnt inhibitor DKK1. Notably, in vivo experiments showed that hPDLSCs overexpressing YTHDC1 exhibited enhanced bone formation capacity in the osteoporotic rats. In conclusion, our findings suggested that YTHDC1 modulated the osteogenic capacity of hPDLSCs through the Wnt/β-catenin signalling pathway, highlighting its therapeutic potential for treating bone defects in osteoporotic conditions.","PeriodicalId":9760,"journal":{"name":"Cell Proliferation","volume":" ","pages":"e70020"},"PeriodicalIF":5.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cutting-Edge Advancements in the Antibiotics-Gut Microbiota-Urinary Tumour Axis. 抗生素-肠道微生物-泌尿肿瘤轴的前沿进展。

IF 5.9 1区生物学

Cell Proliferation Pub Date : 2025-03-17 DOI: 10.1111/cpr.70023

Jie Wang, Dengxiong Li, Ruicheng Wu, Dechao Feng

引用次数: 0

Exploring the Single-Cell Dynamics of FOXM1 Under Cell Cycle Perturbations. 探索细胞周期扰动下FOXM1的单细胞动力学。

IF 5.9 1区生物学

Cell Proliferation Pub Date : 2025-03-17 DOI: 10.1111/cpr.70019

Tooba Jawwad, Maliwan Kamkaew, Kriengkrai Phongkitkarun, Porncheera Chusorn, Supawan Jamnongsong, Eric W-F Lam, Somponnat Sampattavanich

{"title":"Exploring the Single-Cell Dynamics of FOXM1 Under Cell Cycle Perturbations.","authors":"Tooba Jawwad, Maliwan Kamkaew, Kriengkrai Phongkitkarun, Porncheera Chusorn, Supawan Jamnongsong, Eric W-F Lam, Somponnat Sampattavanich","doi":"10.1111/cpr.70019","DOIUrl":"https://doi.org/10.1111/cpr.70019","url":null,"abstract":"The cell cycle is crucial for maintaining normal cellular functions and preventing replication errors. FOXM1, a key transcription factor, plays a pivotal role in regulating cell cycle progression and is implicated in various physiological and pathological processes, including cancers like liver, prostate, breast, lung and colon cancer. Despite previous research, our understanding of FOXM1 dynamics under different cell cycle perturbations and its connection to heterogeneous cell fate decisions remains limited. In this study, we investigated FOXM1 behaviour in individual cells exposed to various perturbagens. We found that different drugs induce diverse responses due to heterogeneous FOXM1 dynamics at the single-cell level. Single-cell analysis identified six distinct cellular phenotypes: on-time cytokinesis, cytokinesis delay, cell cycle delay, G1 arrest, G2 arrest and cell death, observed across different drug types and doses. Specifically, treatments with PLK1, CDK1, CDK1/2 and Aurora kinase inhibitors revealed varied FOXM1 dynamics leading to heterogeneous cellular outcomes. Our findings affirm that the dynamics of FOXM1 are essential in shaping cellular outcomes, influencing the signals that dictate responses to various stimuli. Our results gave insights into how FOXM1 dynamics contribute to cell cycle fate decisions, especially under different cell cycle perturbations.","PeriodicalId":9760,"journal":{"name":"Cell Proliferation","volume":" ","pages":"e70019"},"PeriodicalIF":5.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0